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The map label for this gene is pyrB [H]

Identifier: 21243643

GI number: 21243643

Start: 3424234

End: 3425181

Strand: Reverse

Name: pyrB [H]

Synonym: XAC2916

Alternate gene names: 21243643

Gene position: 3425181-3424234 (Counterclockwise)

Preceding gene: 21243644

Following gene: 21243642

Centisome position: 66.18

GC content: 67.51

Gene sequence:

>948_bases
ATGACCACCATGCAACTCGATTCGGACGGACGCTTGCGCCACCTGCTCACCCTGGAAGGACTTCCGCGCACCACGCTGCT
GCAATTGCTCGACCGCGCCGGCCAGATCCGCGATGCAGCGGTCGGGCGCGTGGGCAAGCGCAGCGTGCTGGCGGGCACTG
CAGTGTGCACGCTGTTCTTCGAGCCCTCCACGCGCACGCGCAGCTCCTTCCATCTGGCGGCGCAGCGGCTGGGCGCGGAC
GTGCTCAATTTCGATGCATCCACCTCATCCACCCGCAAGGGCGAAACCGCGCGCGATACTTTGAAGAATCTCGAAGCGAT
GGGCGTGCGCGGCTTCGTGGTGCGTCACCCCGACGACGGCGCGGTGGAAGCGCTGGCGGCTGCCGCGGGCGAGGGCACCG
CGTTGATCAATGCCGGCGACGGACGTAGCGCGCATCCCACCCAGGGCTTGCTCGACATGCTCACCCTGCGCCAGGCCAAG
GGCACCGATTTTTCCAAGCTCAAGGTCGTTATCGTCGGCGACGTGAAGCACTCGCGCGTGGCGCGCTCGGACCTGCATGC
CTTGCGCACGTTGGGCGCTGGCGAAATCCGCGTCTGCGGCCCGGTTAGCCTGCTGCCTGACGACGGCATGCTGGAGGGCT
GCGTGGTCGGTCAGGATTTCGATGCCATGCTCGAAGGCGCCGACGCGCTGATGATGCTGCGCCTGCAGCGCGAGCGCATG
GAAGAAGGCCTGGTGCCCTCGCTGGAGCAGTACCACACCGAATATGGTCTGACCTGCGAGCGCCTGGCGCGTGCCGGTCG
TGACGCCGCGGTGCTGCACCCGGGCCCGATCAACCGCGGCGTGGAAATCACCGATGAAGTGGCCGACGGCGCGCAATCCT
GCGTGCTACGGCAGGTCGCCAACGGCGTCGCCGTGCGCATGGCCGTGCTGGAAACCCTGCTCGGCTGA

Upstream 100 bases:

>100_bases
GATGCCGAAGCGCTCGATGCGATGGCCGCCGCAGTGATCGTCGAACGCTGGTTGGCCGCACCCGACCAAGCCACCCTTCT
TCCTTGACTCCCGACCTGCG

Downstream 100 bases:

>100_bases
GCGTGGCTGCGCCTCGTTTCGTTAGCGGCCCAGGAGCGGCAACGCGACCGCGCAACCAACGCGCGCGGTCGTGTCTGATG
TGTGATTGTCGATTGCGAAG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTTMQLDSDGRLRHLLTLEGLPRTTLLQLLDRAGQIRDAAVGRVGKRSVLAGTAVCTLFFEPSTRTRSSFHLAAQRLGAD
VLNFDASTSSTRKGETARDTLKNLEAMGVRGFVVRHPDDGAVEALAAAAGEGTALINAGDGRSAHPTQGLLDMLTLRQAK
GTDFSKLKVVIVGDVKHSRVARSDLHALRTLGAGEIRVCGPVSLLPDDGMLEGCVVGQDFDAMLEGADALMMLRLQRERM
EEGLVPSLEQYHTEYGLTCERLARAGRDAAVLHPGPINRGVEITDEVADGAQSCVLRQVANGVAVRMAVLETLLG

Sequences:

>Translated_315_residues
MTTMQLDSDGRLRHLLTLEGLPRTTLLQLLDRAGQIRDAAVGRVGKRSVLAGTAVCTLFFEPSTRTRSSFHLAAQRLGAD
VLNFDASTSSTRKGETARDTLKNLEAMGVRGFVVRHPDDGAVEALAAAAGEGTALINAGDGRSAHPTQGLLDMLTLRQAK
GTDFSKLKVVIVGDVKHSRVARSDLHALRTLGAGEIRVCGPVSLLPDDGMLEGCVVGQDFDAMLEGADALMMLRLQRERM
EEGLVPSLEQYHTEYGLTCERLARAGRDAAVLHPGPINRGVEITDEVADGAQSCVLRQVANGVAVRMAVLETLLG
>Mature_314_residues
TTMQLDSDGRLRHLLTLEGLPRTTLLQLLDRAGQIRDAAVGRVGKRSVLAGTAVCTLFFEPSTRTRSSFHLAAQRLGADV
LNFDASTSSTRKGETARDTLKNLEAMGVRGFVVRHPDDGAVEALAAAAGEGTALINAGDGRSAHPTQGLLDMLTLRQAKG
TDFSKLKVVIVGDVKHSRVARSDLHALRTLGAGEIRVCGPVSLLPDDGMLEGCVVGQDFDAMLEGADALMMLRLQRERME
EGLVPSLEQYHTEYGLTCERLARAGRDAAVLHPGPINRGVEITDEVADGAQSCVLRQVANGVAVRMAVLETLLG

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=312, Percent_Identity=34.9358974358974, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=31.0897435897436, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1786469, Length=345, Percent_Identity=25.7971014492754, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790703, Length=335, Percent_Identity=25.6716417910448, Blast_Score=68, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=293, Percent_Identity=33.1058020477816, Blast_Score=111, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6322331, Length=319, Percent_Identity=33.8557993730408, Blast_Score=142, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24642586, Length=313, Percent_Identity=33.5463258785943, Blast_Score=142, Evalue=2e-34,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 33630; Mature: 33499

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTMQLDSDGRLRHLLTLEGLPRTTLLQLLDRAGQIRDAAVGRVGKRSVLAGTAVCTLFF
CCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHEEEEEEEE
EPSTRTRSSFHLAAQRLGADVLNFDASTSSTRKGETARDTLKNLEAMGVRGFVVRHPDDG
CCCCCCHHHHHHHHHHHCCHHEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCH
AVEALAAAAGEGTALINAGDGRSAHPTQGLLDMLTLRQAKGTDFSKLKVVIVGDVKHSRV
HHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEEECCHHHHH
ARSDLHALRTLGAGEIRVCGPVSLLPDDGMLEGCVVGQDFDAMLEGADALMMLRLQRERM
HHHHHHHHHHCCCCCEEEECCCEECCCCCCCCCEEECCCHHHHHHCHHHHHHHHHHHHHH
EEGLVPSLEQYHTEYGLTCERLARAGRDAAVLHPGPINRGVEITDEVADGAQSCVLRQVA
HCCCCCCHHHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCCEEHHHHHHHHHHHHHHHHH
NGVAVRMAVLETLLG
HHHHHHHHHHHHHHC
>Mature Secondary Structure 
TTMQLDSDGRLRHLLTLEGLPRTTLLQLLDRAGQIRDAAVGRVGKRSVLAGTAVCTLFF
CEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHEEEEEEEE
EPSTRTRSSFHLAAQRLGADVLNFDASTSSTRKGETARDTLKNLEAMGVRGFVVRHPDDG
CCCCCCHHHHHHHHHHHCCHHEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCH
AVEALAAAAGEGTALINAGDGRSAHPTQGLLDMLTLRQAKGTDFSKLKVVIVGDVKHSRV
HHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEEECCHHHHH
ARSDLHALRTLGAGEIRVCGPVSLLPDDGMLEGCVVGQDFDAMLEGADALMMLRLQRERM
HHHHHHHHHHCCCCCEEEECCCEECCCCCCCCCEEECCCHHHHHHCHHHHHHHHHHHHHH
EEGLVPSLEQYHTEYGLTCERLARAGRDAAVLHPGPINRGVEITDEVADGAQSCVLRQVA
HCCCCCCHHHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCCEEHHHHHHHHHHHHHHHHH
NGVAVRMAVLETLLG
HHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA