Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 21243518

GI number: 21243518

Start: 3271850

End: 3272842

Strand: Direct

Name: gcvA [H]

Synonym: XAC2791

Alternate gene names: 21243518

Gene position: 3271850-3272842 (Clockwise)

Preceding gene: 21243516

Following gene: 21243519

Centisome position: 63.22

GC content: 66.67

Gene sequence:

>993_bases
ATGAATCTGCGCCCTACCCTGTTGCCCGCGTTGGGCGTGTTCGCCGCTGCCGCCCGCCACCAGAATTTCGCCCACGCCGC
CGAAGAACTGCACCTTACCGCCAGCGCAGTCAGCCACCATGTGCGCAAGCTCGAAGCGCTGCTGGGTGCGACCTTGTTCC
AGCGGCTACCGCGCGGCGTCAAACTCACCGCCGAAGGCCGCCAGCTGGCCGATGCGGCCACCGCCGCGCTGGCAGAGATC
GCTGCGGTGGCAGGCAACCTGCATCCACGCGAAGACGCCATTCCACTGCGGGTCACCACGTTGCGGTCGTTGTCGTACTG
CTGGTTATTGCCGCGGTTGCCGCGCTTTTGCCGCGCCTATCCGCATATCCGCATGGACCTGCAATCGGATACGGCGTTTT
CGCGTTTCGAAGAAGGCGGCCCGGACCTGGGTATTCGTTATGGCCAAGGCGCTTGGCCCGGGCTAACCTCGCACCATCTG
ATGGACGACGAGCTGTTCCCCGTGGCCTCGCCGTCGTTGCCGGAAGTGGCTGCACTGCGCACGCCGGCGCAGATCGCGCA
ATTGCCGTTACTGACCGATATGTCGCCGCAGGGTTGGCGCGACTGGTTCCGCGCAGCCCAGGTCCGCGGCACCACCGTGC
CGCCGATGCATACCTTCAACGACAGCACCGACGCGATGCGCGCCGCCGTGTATGGCCTGGGCGCGGTGCTGGCGCGCAAG
CACATCGCCCAGCCCTACCTGCAACGTTACGAACTGGTACGCCTGCCCGGCCCCACGCTCAAGGCGCGTTACGCGTACTA
CATCGTGCACCCCAGCCACCGCGAACTCAGCCCGGCGGCGGCATTGTTCATCGACTGGCTCAAGCGCGAAGCACAGGACG
AACGCACCCCGATGCCGGCACTGCCTGCCGAATTGGTGGCGTTGCCGCCGGGCGAAGGCGTGCAGGAGCCGCGTACGCGG
CGGCGTTTGCGGCAAGCACCGGCAACGGAATGA

Upstream 100 bases:

>100_bases
TGGCACGATCGTCGCGAGGGGATGACCCAGCATCGGTTGCCACCGTGGGCGCGACAAGCGCGTAGTATCGGGGCGAGCCA
CAAATCTCACTCATGCCTCG

Downstream 100 bases:

>100_bases
AGGAAGCGGCAATCTGGCCTTCGCTAAGAACGGGGCGATCCCGGCCGCTTCCCGGTGCTGGCACGACGCAGCGACGCCGA
TGGACAGCAAGTGCAGATCG

Product: transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MNLRPTLLPALGVFAAAARHQNFAHAAEELHLTASAVSHHVRKLEALLGATLFQRLPRGVKLTAEGRQLADAATAALAEI
AAVAGNLHPREDAIPLRVTTLRSLSYCWLLPRLPRFCRAYPHIRMDLQSDTAFSRFEEGGPDLGIRYGQGAWPGLTSHHL
MDDELFPVASPSLPEVAALRTPAQIAQLPLLTDMSPQGWRDWFRAAQVRGTTVPPMHTFNDSTDAMRAAVYGLGAVLARK
HIAQPYLQRYELVRLPGPTLKARYAYYIVHPSHRELSPAAALFIDWLKREAQDERTPMPALPAELVALPPGEGVQEPRTR
RRLRQAPATE

Sequences:

>Translated_330_residues
MNLRPTLLPALGVFAAAARHQNFAHAAEELHLTASAVSHHVRKLEALLGATLFQRLPRGVKLTAEGRQLADAATAALAEI
AAVAGNLHPREDAIPLRVTTLRSLSYCWLLPRLPRFCRAYPHIRMDLQSDTAFSRFEEGGPDLGIRYGQGAWPGLTSHHL
MDDELFPVASPSLPEVAALRTPAQIAQLPLLTDMSPQGWRDWFRAAQVRGTTVPPMHTFNDSTDAMRAAVYGLGAVLARK
HIAQPYLQRYELVRLPGPTLKARYAYYIVHPSHRELSPAAALFIDWLKREAQDERTPMPALPAELVALPPGEGVQEPRTR
RRLRQAPATE
>Mature_330_residues
MNLRPTLLPALGVFAAAARHQNFAHAAEELHLTASAVSHHVRKLEALLGATLFQRLPRGVKLTAEGRQLADAATAALAEI
AAVAGNLHPREDAIPLRVTTLRSLSYCWLLPRLPRFCRAYPHIRMDLQSDTAFSRFEEGGPDLGIRYGQGAWPGLTSHHL
MDDELFPVASPSLPEVAALRTPAQIAQLPLLTDMSPQGWRDWFRAAQVRGTTVPPMHTFNDSTDAMRAAVYGLGAVLARK
HIAQPYLQRYELVRLPGPTLKARYAYYIVHPSHRELSPAAALFIDWLKREAQDERTPMPALPAELVALPPGEGVQEPRTR
RRLRQAPATE

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=36.1774744027304, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=36.6782006920415, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=33.7883959044369, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=26.8707482993197, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787128, Length=141, Percent_Identity=31.2056737588652, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787589, Length=291, Percent_Identity=25.085910652921, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786401, Length=283, Percent_Identity=25.0883392226148, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36524; Mature: 36524

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLRPTLLPALGVFAAAARHQNFAHAAEELHLTASAVSHHVRKLEALLGATLFQRLPRGV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
KLTAEGRQLADAATAALAEIAAVAGNLHPREDAIPLRVTTLRSLSYCWLLPRLPRFCRAY
EEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHC
PHIRMDLQSDTAFSRFEEGGPDLGIRYGQGAWPGLTSHHLMDDELFPVASPSLPEVAALR
CCEEEECCCCHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHC
TPAQIAQLPLLTDMSPQGWRDWFRAAQVRGTTVPPMHTFNDSTDAMRAAVYGLGAVLARK
CCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HIAQPYLQRYELVRLPGPTLKARYAYYIVHPSHRELSPAAALFIDWLKREAQDERTPMPA
HHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
LPAELVALPPGEGVQEPRTRRRLRQAPATE
CCHHHEECCCCCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MNLRPTLLPALGVFAAAARHQNFAHAAEELHLTASAVSHHVRKLEALLGATLFQRLPRGV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
KLTAEGRQLADAATAALAEIAAVAGNLHPREDAIPLRVTTLRSLSYCWLLPRLPRFCRAY
EEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHC
PHIRMDLQSDTAFSRFEEGGPDLGIRYGQGAWPGLTSHHLMDDELFPVASPSLPEVAALR
CCEEEECCCCHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHC
TPAQIAQLPLLTDMSPQGWRDWFRAAQVRGTTVPPMHTFNDSTDAMRAAVYGLGAVLARK
CCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HIAQPYLQRYELVRLPGPTLKARYAYYIVHPSHRELSPAAALFIDWLKREAQDERTPMPA
HHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
LPAELVALPPGEGVQEPRTRRRLRQAPATE
CCHHHEECCCCCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]