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The map label for this gene is dniR [H]

Identifier: 21243456

GI number: 21243456

Start: 3190523

End: 3191935

Strand: Reverse

Name: dniR [H]

Synonym: XAC2729

Alternate gene names: 21243456

Gene position: 3191935-3190523 (Counterclockwise)

Preceding gene: 21243458

Following gene: 21243453

Centisome position: 61.67

GC content: 65.96

Gene sequence:

>1413_bases
GTGAACAACTCCGACCCCAAGGGCACTGCCGAAGGCGATGCCGCGCTGGAGGACATGGAAGACATCATCGACGCCTGCAT
CAAGCAGCGCGGTTGCCTGGTGTCCAATCAGTTGGCCACCTACAAGCGACTGCTCAAGGCGCGCGCCGATGCCGAGGCGC
CGGCCGCCGAAGATCCGGAAGACGACGACACCCTGCTGCAAGCCGACCCGGACCATCTCGGCCCGGCGTCGAACGGCGTG
CCCGAGGCCGCGCGTGCCGCCACGCTGCTCAACGACCAGCGCCACGACTTCGACAAGATGGTGCAGTACAACCCGGCCGT
GCAGGCCGGTATCCGGCGTTGGCTCACCGATATGCGCCCGGCGCTGCTGACCAGCTACGAGAACTACAGCAACCTGCGCG
GCGTGATGTGGCCGGAATGGCAGAAGCGCGGCCTTCCCGAAGCGTTGCTGTTCGGCATCATGGCCAAGGAATCCAACGGC
AAGGTGCACGCCAGCTCGCGCGCTGGTGCAGCCGGGTTGATGCAGTTCATGCCCGCCACCGGACGTCGCTTCGGGCTGGG
CCCGGACGGCACCGGCTTCGATACGCGCTTCGATGCACGCAGCGCCGCCGAAGCCAGCGCCGCCTACCTCAACGAGCGCA
TGGCCGGGCTTAACCGAAGCATCGAACTGGCGCTGGCCGCCTACAACGGTGGCGAAGGCCGTGCCGCGCGCGTGTTTTCG
CAGAGCGGCGGGCGCGGCTTCTGGGATCAGGACGTCTACAACCAGTTCCCGGCCGAAACCAAGGACTACGTGCCGATGGT
GATCGCGGCGGCGTGGATCTTCCTGCACCCGCGCCAGTATGGGGTGGACTTCCCCAAGCTCGATGCGCAGCCGGCCACGC
TCAAGCTGGCCAAGTCGACCACCATCTACGAACTCACCATCTGCCTGGGCAGCATGGGTACACGCGACGGCTACATGCGT
GCGCTGCGCAATCTCAACCCGCGTTACGAATCCGATGGCTGGATTCCGGCCGGCACGGTCATCAATGCCACCAACCGCAT
CGTCAACCTCTACACCCGTTACTGCGTCAATGGCCCGCGTGCGGATCTGGCGCGCACCCTGATCACTGCCGATCTCAACA
GCGCGATCGTGCGCAGCAGCAGCGCGCCCGAGTACGTGCCAAGCGTGGCGGTGGGCGATGTCAGCCAGATGGCGGGCGTC
CCGACCACCGTCGCCACCGGTCAGCCGGCAGTTGCCAAGCCCAAGGCCAAGCAGGTGCGCAGCTACACCATCGCCCGGGG
CGACACGCTCGGTCGCGTTGCGCAGAAATACCAATGCGAGATCAAGGAGCTGGCCAAGGCCAACGGCTTGAAGGCGCCGT
CGTATGCGCTCAAGCCGGGTCAGAGCATCAAACTGGCGGGTTGCGATCGCTGA

Upstream 100 bases:

>100_bases
CGCCGCCCACGCCATCTCCAAACGCGACACGGAGAAGGCCGCCGCGCTCGATGCGCGCATGGCCGCCGCCGAAAAGCGCT
ATCGCGATGCGCTGGTGCTG

Downstream 100 bases:

>100_bases
ACCGTGCGCTGGCGGTGCCGTGCTGATCGCCGCACCCTCTAGACGGCGGTGCGCATCGGCCTGAACCCTGGCGTGGGGCA
GGCCAGCCGCGCGAACGCCG

Product: membrane-bound lytic murein transglycosylase D

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MNNSDPKGTAEGDAALEDMEDIIDACIKQRGCLVSNQLATYKRLLKARADAEAPAAEDPEDDDTLLQADPDHLGPASNGV
PEAARAATLLNDQRHDFDKMVQYNPAVQAGIRRWLTDMRPALLTSYENYSNLRGVMWPEWQKRGLPEALLFGIMAKESNG
KVHASSRAGAAGLMQFMPATGRRFGLGPDGTGFDTRFDARSAAEASAAYLNERMAGLNRSIELALAAYNGGEGRAARVFS
QSGGRGFWDQDVYNQFPAETKDYVPMVIAAAWIFLHPRQYGVDFPKLDAQPATLKLAKSTTIYELTICLGSMGTRDGYMR
ALRNLNPRYESDGWIPAGTVINATNRIVNLYTRYCVNGPRADLARTLITADLNSAIVRSSSAPEYVPSVAVGDVSQMAGV
PTTVATGQPAVAKPKAKQVRSYTIARGDTLGRVAQKYQCEIKELAKANGLKAPSYALKPGQSIKLAGCDR

Sequences:

>Translated_470_residues
MNNSDPKGTAEGDAALEDMEDIIDACIKQRGCLVSNQLATYKRLLKARADAEAPAAEDPEDDDTLLQADPDHLGPASNGV
PEAARAATLLNDQRHDFDKMVQYNPAVQAGIRRWLTDMRPALLTSYENYSNLRGVMWPEWQKRGLPEALLFGIMAKESNG
KVHASSRAGAAGLMQFMPATGRRFGLGPDGTGFDTRFDARSAAEASAAYLNERMAGLNRSIELALAAYNGGEGRAARVFS
QSGGRGFWDQDVYNQFPAETKDYVPMVIAAAWIFLHPRQYGVDFPKLDAQPATLKLAKSTTIYELTICLGSMGTRDGYMR
ALRNLNPRYESDGWIPAGTVINATNRIVNLYTRYCVNGPRADLARTLITADLNSAIVRSSSAPEYVPSVAVGDVSQMAGV
PTTVATGQPAVAKPKAKQVRSYTIARGDTLGRVAQKYQCEIKELAKANGLKAPSYALKPGQSIKLAGCDR
>Mature_470_residues
MNNSDPKGTAEGDAALEDMEDIIDACIKQRGCLVSNQLATYKRLLKARADAEAPAAEDPEDDDTLLQADPDHLGPASNGV
PEAARAATLLNDQRHDFDKMVQYNPAVQAGIRRWLTDMRPALLTSYENYSNLRGVMWPEWQKRGLPEALLFGIMAKESNG
KVHASSRAGAAGLMQFMPATGRRFGLGPDGTGFDTRFDARSAAEASAAYLNERMAGLNRSIELALAAYNGGEGRAARVFS
QSGGRGFWDQDVYNQFPAETKDYVPMVIAAAWIFLHPRQYGVDFPKLDAQPATLKLAKSTTIYELTICLGSMGTRDGYMR
ALRNLNPRYESDGWIPAGTVINATNRIVNLYTRYCVNGPRADLARTLITADLNSAIVRSSSAPEYVPSVAVGDVSQMAGV
PTTVATGQPAVAKPKAKQVRSYTIARGDTLGRVAQKYQCEIKELAKANGLKAPSYALKPGQSIKLAGCDR

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=356, Percent_Identity=28.6516853932584, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 50933; Mature: 50933

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSDPKGTAEGDAALEDMEDIIDACIKQRGCLVSNQLATYKRLLKARADAEAPAAEDPE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
DDDTLLQADPDHLGPASNGVPEAARAATLLNDQRHDFDKMVQYNPAVQAGIRRWLTDMRP
CCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALLTSYENYSNLRGVMWPEWQKRGLPEALLFGIMAKESNGKVHASSRAGAAGLMQFMPAT
HHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHEECCCCCEEECCCCCHHHHHHHHCCC
GRRFGLGPDGTGFDTRFDARSAAEASAAYLNERMAGLNRSIELALAAYNGGEGRAARVFS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEHHHH
QSGGRGFWDQDVYNQFPAETKDYVPMVIAAAWIFLHPRQYGVDFPKLDAQPATLKLAKST
CCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEECCC
TIYELTICLGSMGTRDGYMRALRNLNPRYESDGWIPAGTVINATNRIVNLYTRYCVNGPR
EEEEEEEHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCH
ADLARTLITADLNSAIVRSSSAPEYVPSVAVGDVSQMAGVPTTVATGQPAVAKPKAKQVR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCHHHHCCCCCEECCCCCCCCCCHHHHHH
SYTIARGDTLGRVAQKYQCEIKELAKANGLKAPSYALKPGQSIKLAGCDR
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
>Mature Secondary Structure
MNNSDPKGTAEGDAALEDMEDIIDACIKQRGCLVSNQLATYKRLLKARADAEAPAAEDPE
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
DDDTLLQADPDHLGPASNGVPEAARAATLLNDQRHDFDKMVQYNPAVQAGIRRWLTDMRP
CCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALLTSYENYSNLRGVMWPEWQKRGLPEALLFGIMAKESNGKVHASSRAGAAGLMQFMPAT
HHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHEECCCCCEEECCCCCHHHHHHHHCCC
GRRFGLGPDGTGFDTRFDARSAAEASAAYLNERMAGLNRSIELALAAYNGGEGRAARVFS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEHHHH
QSGGRGFWDQDVYNQFPAETKDYVPMVIAAAWIFLHPRQYGVDFPKLDAQPATLKLAKST
CCCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEECCC
TIYELTICLGSMGTRDGYMRALRNLNPRYESDGWIPAGTVINATNRIVNLYTRYCVNGPR
EEEEEEEHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCH
ADLARTLITADLNSAIVRSSSAPEYVPSVAVGDVSQMAGVPTTVATGQPAVAKPKAKQVR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCHHHHCCCCCEECCCCCCCCCCHHHHHH
SYTIARGDTLGRVAQKYQCEIKELAKANGLKAPSYALKPGQSIKLAGCDR
HHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]