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The map label for this gene is tpiA

Identifier: 21243434

GI number: 21243434

Start: 3166135

End: 3166890

Strand: Reverse

Name: tpiA

Synonym: XAC2707

Alternate gene names: 21243434

Gene position: 3166890-3166135 (Counterclockwise)

Preceding gene: 21243436

Following gene: 161485710

Centisome position: 61.19

GC content: 66.01

Gene sequence:

>756_bases
ATGCGTCGAAAGATCGTTGCCGGAAATTGGAAGCTGCATGGCAGCCGCGCCTTCGCCACCGAACTGGTGGCCAAGGTGGC
CGCGCACATGCCCCTGGAGGGTGTCGAAGTCGTCATCCTGCCGCCACTGCCTTACCTCGGCGACCTGATCGAGGATTTCG
AGGCCCATCACCTGTCGTTCGGAGCCCAGGACGTCAGCAGTAACGAGAAGGGCGCCTACACCGGCGAGGTATCGGCCTCG
ATGCTGGTCGATGTCGGTGCTGGCTACGGGCTGGTCGGCCACTCCGAGCGCCGCCAATACCATCAGGAGAGTAGCGAACT
GGTAGCGCGCAAGTTCGCCGCCGCCCTCCATGCCGGGCTGATTCCGGTGCTGTGCGTGGGCGAATCGCTGGAACAGCGTG
AAGCCGGTCAGACTGAGGCAATCCTACGTGCCCAGCTCGACCCGGTCCTGGCGCTGGTGGGCAGTGCCGGTTTTGCGGGC
GCGGTGCTGGCCTACGAACCGATCTGGGCCATTGGCACCGGCCGCACCGCCACCCCGGAGCAGGCCCAGGCGGTGCACGC
TTTCCTGCGTGGCGAAGTCGCGAAGGCGGATGCTAGAATCGCCGATTCGTTGCCCATCCTGTACGGGGGCAGTGTCAAGC
CCGACAACGCCGGCGAGCTGTTCGCACAGCCTGACGTCGATGGCGGGCTGGTCGGAGGCGCATCACTGGTCGCCGAAGAT
TTCCTGGCCATCGCACGCGCGGCGGCCGCGTGCTAA

Upstream 100 bases:

>100_bases
TAAGGGGAGCGGGAGGACATCTGGCGGACAGAAGTGTGCCGGTTGCCGACAGGCCTTGGGCTATTCTGTCGCCTGTCTTC
AACTCAGGAACCTGCGCCTG

Downstream 100 bases:

>100_bases
CCCATTAAGTTTTGTCCGGGGACGGATTTCGAAATGCTGATGTTGATCCTCAATGTGGTCTACGTGCTGGTCGCCCTGGC
GATGATTGCGCTGATCCTGA

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MRRKIVAGNWKLHGSRAFATELVAKVAAHMPLEGVEVVILPPLPYLGDLIEDFEAHHLSFGAQDVSSNEKGAYTGEVSAS
MLVDVGAGYGLVGHSERRQYHQESSELVARKFAAALHAGLIPVLCVGESLEQREAGQTEAILRAQLDPVLALVGSAGFAG
AVLAYEPIWAIGTGRTATPEQAQAVHAFLRGEVAKADARIADSLPILYGGSVKPDNAGELFAQPDVDGGLVGGASLVAED
FLAIARAAAAC

Sequences:

>Translated_251_residues
MRRKIVAGNWKLHGSRAFATELVAKVAAHMPLEGVEVVILPPLPYLGDLIEDFEAHHLSFGAQDVSSNEKGAYTGEVSAS
MLVDVGAGYGLVGHSERRQYHQESSELVARKFAAALHAGLIPVLCVGESLEQREAGQTEAILRAQLDPVLALVGSAGFAG
AVLAYEPIWAIGTGRTATPEQAQAVHAFLRGEVAKADARIADSLPILYGGSVKPDNAGELFAQPDVDGGLVGGASLVAED
FLAIARAAAAC
>Mature_251_residues
MRRKIVAGNWKLHGSRAFATELVAKVAAHMPLEGVEVVILPPLPYLGDLIEDFEAHHLSFGAQDVSSNEKGAYTGEVSAS
MLVDVGAGYGLVGHSERRQYHQESSELVARKFAAALHAGLIPVLCVGESLEQREAGQTEAILRAQLDPVLALVGSAGFAG
AVLAYEPIWAIGTGRTATPEQAQAVHAFLRGEVAKADARIADSLPILYGGSVKPDNAGELFAQPDVDGGLVGGASLVAED
FLAIARAAAAC

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=249, Percent_Identity=44.9799196787149, Blast_Score=183, Evalue=1e-46,
Organism=Homo sapiens, GI226529917, Length=249, Percent_Identity=44.9799196787149, Blast_Score=182, Evalue=2e-46,
Organism=Escherichia coli, GI1790353, Length=251, Percent_Identity=54.1832669322709, Blast_Score=271, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17536593, Length=247, Percent_Identity=44.1295546558704, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6320255, Length=239, Percent_Identity=43.5146443514644, Blast_Score=179, Evalue=2e-46,
Organism=Drosophila melanogaster, GI28572008, Length=247, Percent_Identity=42.914979757085, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28572006, Length=247, Percent_Identity=42.914979757085, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI28572004, Length=247, Percent_Identity=42.914979757085, Blast_Score=178, Evalue=2e-45,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_XANAC (Q8PJ38)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_643016.1
- ProteinModelPortal:   Q8PJ38
- SMR:   Q8PJ38
- GeneID:   1156778
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC2707
- NMPDR:   fig|190486.1.peg.2660
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   XAXO190486:XAC2707-MONOMER
- BRENDA:   5.3.1.1
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26188; Mature: 26188

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 94-94 ACT_SITE 166-166 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKIVAGNWKLHGSRAFATELVAKVAAHMPLEGVEVVILPPLPYLGDLIEDFEAHHLSF
CCCCEEECCEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHC
GAQDVSSNEKGAYTGEVSASMLVDVGAGYGLVGHSERRQYHQESSELVARKFAAALHAGL
CCHHCCCCCCCCEECCCHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
IPVLCVGESLEQREAGQTEAILRAQLDPVLALVGSAGFAGAVLAYEPIWAIGTGRTATPE
HHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHEECCEEEECCCCCCCHH
QAQAVHAFLRGEVAKADARIADSLPILYGGSVKPDNAGELFAQPDVDGGLVGGASLVAED
HHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHH
FLAIARAAAAC
HHHHHHHHHCC
>Mature Secondary Structure
MRRKIVAGNWKLHGSRAFATELVAKVAAHMPLEGVEVVILPPLPYLGDLIEDFEAHHLSF
CCCCEEECCEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHC
GAQDVSSNEKGAYTGEVSASMLVDVGAGYGLVGHSERRQYHQESSELVARKFAAALHAGL
CCHHCCCCCCCCEECCCHHHHEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
IPVLCVGESLEQREAGQTEAILRAQLDPVLALVGSAGFAGAVLAYEPIWAIGTGRTATPE
HHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCHHHEECCEEEECCCCCCCHH
QAQAVHAFLRGEVAKADARIADSLPILYGGSVKPDNAGELFAQPDVDGGLVGGASLVAED
HHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEECCCCCCCCCCHHHHHHHH
FLAIARAAAAC
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217