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The map label for this gene is nusA [H]

Identifier: 21243417

GI number: 21243417

Start: 3148007

End: 3149518

Strand: Reverse

Name: nusA [H]

Synonym: XAC2688

Alternate gene names: 21243417

Gene position: 3149518-3148007 (Counterclockwise)

Preceding gene: 21243418

Following gene: 21243416

Centisome position: 60.85

GC content: 64.48

Gene sequence:

>1512_bases
ATGAGCAAGGAACTTTTGCTGGTAGTGGATGCGGTCGCCAACGAGAAGGGCGTGCCGCGCGAGGTGATCTTCGAAGCGAT
CGAGGCCGCACTGGCTTCCGCAGCGAAGAAGCGCTATCCCGACCAGGACGTGCTGGCCCGCGTCACCATCGATCACAAGG
ACGGTACTTACGAAACCTACCGTCGCTGGGAAGTGGTTGCCGATGATGTGGTGATGGAATCCCCTGACCGCCAGGTGCGT
CTGATGGATGCCATCGACGAAGCCGATGGCGTGGAAGTGGGTGATTACATCGAAGAACAGATCGAGAATCCCGACTTCGG
CCGTATCGCCGCGCAGGCCGCCAAGCAGGTGATCGTGCAGCGTGTGCGCGAGGCCGAGCGTCAGCAGGTGGTGGATGCGT
GGAAGGATCGTGTGGGTGAGTTGATCACCGGCGTGGTCAAGCGCGCAGAGCGCGGCAATATTTTTGTCGATCTTGGCGGC
AACGCCGAAGCCTTCATTCCGAAGGACAAGGGCATTCCGCGCGACGTGCTGCGTCCCGGCGACCGCGTGCGCGGTTATCT
GGCCGAAGTGCGCTCCGAGCCACGTGGCCCGCAGCTGTTCGTCAGCCGTGCCGCACCGGAATTCATGATCGAACTGTTCA
AGCTCGAAGTGCCGGAGGTCGGCCAGGGCCTGGTCGAGATCAAGGCTTGCGCACGCGATCCGGGCGACCGCGCCAAGATT
GCCGTGATCGCGCACGACGCCCGCACCGACCCCATCGGCGCATGCATCGGCATGCGCGGTTCGCGCGTGCAAGCGGTGTC
CAACGAACTCAATGGCGAGCGCGTGGACATCGTGTTGTGGAACGAGAACCCGGCCAACTTCGTCATCAACGCAATGGCGC
CTGCCGAAGTGCAGTCGATCATCGTCGATGAAGACAAGCACTCGATGGACCTGGCCGTGGCGGAAGACCGCCTTGCGCAG
GCGATCGGCAAGGGCGGCCAGAACGTGCGTCTGGCCAGCCGCCTGACCGGTTGGCAGCTCAACGTGATGACCGCCGACCA
GGTGGCCGCCAAGTCCGAGGCCGAGCAGGCCGCGGCGCGCCAGCTGTTCATGGACCGCCTGGAAGTGGACGAGGAAATCT
CCGCGATCCTGGTCTCCGAAGGTTTCAACACGGTCGAAGAAATCGCCTACGTGCCGGTCGGCGAGTTGCTGGCGGTGGAG
GGCTTCGACGAAGACATCGTTGAAGAACTGCGCGCCCGTGCCCGCGATGCGCTGCTGAACGCCGCGCTGGCCGAGGAGGA
GGGCCTGGAAGGAACTCAGCCCACCGAGGACCTGCTGGCGCTGGAGGGGATGGACGAGGAAACGGCCTTTGCGCTGGCCG
AGCACGGCGTGCGCACCAGCGAGGACTTGTCCGACCTGGCAGCGGACGAGATCGTCGACTTCGGCATTGAGGGCATGACC
CAGGAGCGCGCTGCGGCGCTGATCCTGGCGGCCCGCGCCGAGGAGATCGCCCGTTTGGAGCGCGGCGAATGA

Upstream 100 bases:

>100_bases
CCGCAGAAACCGAACAAGCCCGGCCCGAAAAAACCCGGGCACGAAAAGAAGAAACCATCCAACGAGTCGGCGGCTGGCAA
GCCGCGCGCGGAGTGACGAA

Downstream 100 bases:

>100_bases
GCCGGCAATGAACCGCGCCCTGACCCCATCAGGGCTTAGAATCAGCGTATCCCTTGCTCTGGGCGAGGGGGCGCCAACCA
GATCATAGGATCCGAATGTC

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: L factor; N utilization substance protein A [H]

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MSKELLLVVDAVANEKGVPREVIFEAIEAALASAAKKRYPDQDVLARVTIDHKDGTYETYRRWEVVADDVVMESPDRQVR
LMDAIDEADGVEVGDYIEEQIENPDFGRIAAQAAKQVIVQRVREAERQQVVDAWKDRVGELITGVVKRAERGNIFVDLGG
NAEAFIPKDKGIPRDVLRPGDRVRGYLAEVRSEPRGPQLFVSRAAPEFMIELFKLEVPEVGQGLVEIKACARDPGDRAKI
AVIAHDARTDPIGACIGMRGSRVQAVSNELNGERVDIVLWNENPANFVINAMAPAEVQSIIVDEDKHSMDLAVAEDRLAQ
AIGKGGQNVRLASRLTGWQLNVMTADQVAAKSEAEQAAARQLFMDRLEVDEEISAILVSEGFNTVEEIAYVPVGELLAVE
GFDEDIVEELRARARDALLNAALAEEEGLEGTQPTEDLLALEGMDEETAFALAEHGVRTSEDLSDLAADEIVDFGIEGMT
QERAAALILAARAEEIARLERGE

Sequences:

>Translated_503_residues
MSKELLLVVDAVANEKGVPREVIFEAIEAALASAAKKRYPDQDVLARVTIDHKDGTYETYRRWEVVADDVVMESPDRQVR
LMDAIDEADGVEVGDYIEEQIENPDFGRIAAQAAKQVIVQRVREAERQQVVDAWKDRVGELITGVVKRAERGNIFVDLGG
NAEAFIPKDKGIPRDVLRPGDRVRGYLAEVRSEPRGPQLFVSRAAPEFMIELFKLEVPEVGQGLVEIKACARDPGDRAKI
AVIAHDARTDPIGACIGMRGSRVQAVSNELNGERVDIVLWNENPANFVINAMAPAEVQSIIVDEDKHSMDLAVAEDRLAQ
AIGKGGQNVRLASRLTGWQLNVMTADQVAAKSEAEQAAARQLFMDRLEVDEEISAILVSEGFNTVEEIAYVPVGELLAVE
GFDEDIVEELRARARDALLNAALAEEEGLEGTQPTEDLLALEGMDEETAFALAEHGVRTSEDLSDLAADEIVDFGIEGMT
QERAAALILAARAEEIARLERGE
>Mature_502_residues
SKELLLVVDAVANEKGVPREVIFEAIEAALASAAKKRYPDQDVLARVTIDHKDGTYETYRRWEVVADDVVMESPDRQVRL
MDAIDEADGVEVGDYIEEQIENPDFGRIAAQAAKQVIVQRVREAERQQVVDAWKDRVGELITGVVKRAERGNIFVDLGGN
AEAFIPKDKGIPRDVLRPGDRVRGYLAEVRSEPRGPQLFVSRAAPEFMIELFKLEVPEVGQGLVEIKACARDPGDRAKIA
VIAHDARTDPIGACIGMRGSRVQAVSNELNGERVDIVLWNENPANFVINAMAPAEVQSIIVDEDKHSMDLAVAEDRLAQA
IGKGGQNVRLASRLTGWQLNVMTADQVAAKSEAEQAAARQLFMDRLEVDEEISAILVSEGFNTVEEIAYVPVGELLAVEG
FDEDIVEELRARARDALLNAALAEEEGLEGTQPTEDLLALEGMDEETAFALAEHGVRTSEDLSDLAADEIVDFGIEGMTQ
ERAAALILAARAEEIARLERGE

Specific function: Participates in both the termination and antitermination of transcription. NusA binds directly to the core enzyme of the DNA-dependent RNA polymerase. NusA also interacts with lambda N protein, RNA, rho factor, and perhaps nusB. Binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=492, Percent_Identity=58.130081300813, Blast_Score=559, Evalue=1e-160,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 55126; Mature: 54994

Theoretical pI: Translated: 4.19; Mature: 4.19

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKELLLVVDAVANEKGVPREVIFEAIEAALASAAKKRYPDQDVLARVTIDHKDGTYETY
CCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCHHHH
RRWEVVADDVVMESPDRQVRLMDAIDEADGVEVGDYIEEQIENPDFGRIAAQAAKQVIVQ
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RVREAERQQVVDAWKDRVGELITGVVKRAERGNIFVDLGGNAEAFIPKDKGIPRDVLRPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCCCHHHHCCC
DRVRGYLAEVRSEPRGPQLFVSRAAPEFMIELFKLEVPEVGQGLVEIKACARDPGDRAKI
HHHHHHHHHHHCCCCCCEEEEHHCCHHHHHHHHHCCCCHHCCHHHHHHHHHCCCCCCCEE
AVIAHDARTDPIGACIGMRGSRVQAVSNELNGERVDIVLWNENPANFVINAMAPAEVQSI
EEEEECCCCCCHHHHHCCCCCHHHHHHHCCCCCEEEEEEECCCCCCEEEECCCHHHHHHH
IVDEDKHSMDLAVAEDRLAQAIGKGGQNVRLASRLTGWQLNVMTADQVAAKSEAEQAAAR
HCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHCCCCEEEEEEHHHHHHHHHHHHHHHH
QLFMDRLEVDEEISAILVSEGFNTVEEIAYVPVGELLAVEGFDEDIVEELRARARDALLN
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHEEECCCCHHHHHHHHHHHHHHHHH
AALAEEEGLEGTQPTEDLLALEGMDEETAFALAEHGVRTSEDLSDLAADEIVDFGIEGMT
HHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
QERAAALILAARAEEIARLERGE
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKELLLVVDAVANEKGVPREVIFEAIEAALASAAKKRYPDQDVLARVTIDHKDGTYETY
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEECCCCCHHHH
RRWEVVADDVVMESPDRQVRLMDAIDEADGVEVGDYIEEQIENPDFGRIAAQAAKQVIVQ
HHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RVREAERQQVVDAWKDRVGELITGVVKRAERGNIFVDLGGNAEAFIPKDKGIPRDVLRPG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEECCCCCCCCHHHHCCC
DRVRGYLAEVRSEPRGPQLFVSRAAPEFMIELFKLEVPEVGQGLVEIKACARDPGDRAKI
HHHHHHHHHHHCCCCCCEEEEHHCCHHHHHHHHHCCCCHHCCHHHHHHHHHCCCCCCCEE
AVIAHDARTDPIGACIGMRGSRVQAVSNELNGERVDIVLWNENPANFVINAMAPAEVQSI
EEEEECCCCCCHHHHHCCCCCHHHHHHHCCCCCEEEEEEECCCCCCEEEECCCHHHHHHH
IVDEDKHSMDLAVAEDRLAQAIGKGGQNVRLASRLTGWQLNVMTADQVAAKSEAEQAAAR
HCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEHHHCCCCEEEEEEHHHHHHHHHHHHHHHH
QLFMDRLEVDEEISAILVSEGFNTVEEIAYVPVGELLAVEGFDEDIVEELRARARDALLN
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHEEECCCCHHHHHHHHHHHHHHHHH
AALAEEEGLEGTQPTEDLLALEGMDEETAFALAEHGVRTSEDLSDLAADEIVDFGIEGMT
HHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
QERAAALILAARAEEIARLERGE
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113180; 11677609 [H]