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The map label for this gene is amiC [H]

Identifier: 21243140

GI number: 21243140

Start: 2802985

End: 2804712

Strand: Reverse

Name: amiC [H]

Synonym: XAC2406

Alternate gene names: 21243140

Gene position: 2804712-2802985 (Counterclockwise)

Preceding gene: 21243141

Following gene: 21243139

Centisome position: 54.19

GC content: 67.77

Gene sequence:

>1728_bases
ATGCTTGAATCCAGCGCCATGACACCGGGGATCCGCAACTTCTGTCTTTCCGCCCTGACCGCCAGCTTGAGCCTGGCGGT
TTTTGCTGCCTGGGGCGGCGAAATCAAGGGGGTCGGCGTCAGTACCGGAGCCACCGGGACCCGTGCGGAGATTCAACTGG
CCGGCAGCGGCGGCTTCAAAACGCTGTCGCTGGCGAACCCGACGCGCCTGGTCGTGGATTTTCCAGAATCCTCGGGCGTG
CGCGGTCTCAAGCTGCCGGCTGCTGCCGGATTGGTGACCTCGGTGCGCACCGGTCAGCCGGTGCCTGGCACGTTCCGTGT
GGTGTTCGAACTGGCAACGCCGGTGACCCCGCTGAAACCGCAGATGCAGACGCTGGGCAGCGCGTCGACATTGGTGATCG
AATGGCCCGGCGATCCGACGCCGGCGGCGGCCTCCGCAGTGGCTGCGGCTGCACCGACGGCAGCCCCGGCGCCGCGCCCG
CTCAATGCACAGGCCGAAGCGGCACGCGCGACTGCAGCCTTGGCCGCATCTGCGCAACGTGCCTCGTCGGTGCCGCCGCC
TCAGCCGTCGACGCCGCCGCCTGCCCCGTCTGTGCCGGCATCGGCGATGCCCACTGTCACTCAGGCCCCGGTACCGACCA
CCATCGCCACTGGTGTGCCGACACCTCGGCCTGCCACCTCCGCGACCACCGGTGCGCCAGCGCCGACTGGCGTTGCCGGC
AACACGCCGAACCGTGCCGCCGGGGCCGCCGCTGCCGTACCGTCTGGCGCAGTGGTGGCGGGCAGTAGCGCAGCTGCAGC
TGCGATCTTGAATGGCGGCAGCGCACCGATGGGTGCAACGTCCGGTAATGCCGGAGCAATCGCCCCCAATAGTGCTTCGG
GCGTGGTGGCTGCCGCAGGCGACGACGACCTGCCGCCGCGTCCGGTACTGCCCAGCGAAGCCTCGCGGATCAAGATGGCA
CCGGGCATGCGGCCATTGGTCGTGGCGATCGACCCAGGCCACGGCGGCCAGGACCCGGGCGCCATGGGGCCCACCGGTAA
GCGCGAGAAGGACGTCACCCTGGCGGTGGGTCGTGAACTGGCACGCCAGGTCAACGCCACGCCAGGCATGAAGGCCTACC
TGACGCGCGATACCGACGTGTTCATTCCATTGCCGATGCGTGCGCAGAAAGCCCGCGCTGCCAAGGCCGACATCTTCATT
TCCATCCATGCCGACGCCGCCGAAAACCGCAGCGCCACTGGCTCCTCCGTGTACGTACTGTCGACCAAGGGAGCCTCGTC
GCAACGGGCGCGCTGGCTGGCGGACAAGGAAAACGCCGCCGATCTGGTTGGCGGCGTGCGCCTGCAGCAAACCGAAAGCA
CCCTGGCCAACGTGTTGTTGGACCTGGCGCAGAGCGGCCACATGAAGGCCTCCGAAGATGCCGCTGGCCATGTATTGGGT
GGGCTCAAGCGCATCGGCAACAATCACAAGCCGCAATTGGAACGCGCCAACTTCGCGGTGCTGCGGACCTCCGACATGCC
GGCGATGCTGGTCGAAACTGCCTTCATCTCCAATCCGGATGAAGAACGTCGCCTGATCGATCCGGCTTACCAGCGCAGGA
TTGCCAGCGCCGTGCTGGACGGCATCGATACCTTCTTTACCCGCCAACCGCCGCCAGGCACCTTGTTCGCCGCGCGTGCG
CAGGCCGAAGCCGATGCAGTCGGCACCGTGGCCGGCGGCAGCCGCTGA

Upstream 100 bases:

>100_bases
CGGCAACCCCAGTTGCAGGTCCTTTTTGCCGCCTCACGAGAAGAATGAACGCCAGGTTATGGATTATTAAGGGAAGTTGC
CTTGCAATTCACGCAGTTGG

Downstream 100 bases:

>100_bases
CCGGCTCGGCTATCATTCCGCACTGATTCCTTAGATGACGGCGTCTGCGCGCCGCCGGATTGCCGTGCCGATGTCCGTCT
TGTCTGTCACACCGCCGATC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MLESSAMTPGIRNFCLSALTASLSLAVFAAWGGEIKGVGVSTGATGTRAEIQLAGSGGFKTLSLANPTRLVVDFPESSGV
RGLKLPAAAGLVTSVRTGQPVPGTFRVVFELATPVTPLKPQMQTLGSASTLVIEWPGDPTPAAASAVAAAAPTAAPAPRP
LNAQAEAARATAALAASAQRASSVPPPQPSTPPPAPSVPASAMPTVTQAPVPTTIATGVPTPRPATSATTGAPAPTGVAG
NTPNRAAGAAAAVPSGAVVAGSSAAAAAILNGGSAPMGATSGNAGAIAPNSASGVVAAAGDDDLPPRPVLPSEASRIKMA
PGMRPLVVAIDPGHGGQDPGAMGPTGKREKDVTLAVGRELARQVNATPGMKAYLTRDTDVFIPLPMRAQKARAAKADIFI
SIHADAAENRSATGSSVYVLSTKGASSQRARWLADKENAADLVGGVRLQQTESTLANVLLDLAQSGHMKASEDAAGHVLG
GLKRIGNNHKPQLERANFAVLRTSDMPAMLVETAFISNPDEERRLIDPAYQRRIASAVLDGIDTFFTRQPPPGTLFAARA
QAEADAVGTVAGGSR

Sequences:

>Translated_575_residues
MLESSAMTPGIRNFCLSALTASLSLAVFAAWGGEIKGVGVSTGATGTRAEIQLAGSGGFKTLSLANPTRLVVDFPESSGV
RGLKLPAAAGLVTSVRTGQPVPGTFRVVFELATPVTPLKPQMQTLGSASTLVIEWPGDPTPAAASAVAAAAPTAAPAPRP
LNAQAEAARATAALAASAQRASSVPPPQPSTPPPAPSVPASAMPTVTQAPVPTTIATGVPTPRPATSATTGAPAPTGVAG
NTPNRAAGAAAAVPSGAVVAGSSAAAAAILNGGSAPMGATSGNAGAIAPNSASGVVAAAGDDDLPPRPVLPSEASRIKMA
PGMRPLVVAIDPGHGGQDPGAMGPTGKREKDVTLAVGRELARQVNATPGMKAYLTRDTDVFIPLPMRAQKARAAKADIFI
SIHADAAENRSATGSSVYVLSTKGASSQRARWLADKENAADLVGGVRLQQTESTLANVLLDLAQSGHMKASEDAAGHVLG
GLKRIGNNHKPQLERANFAVLRTSDMPAMLVETAFISNPDEERRLIDPAYQRRIASAVLDGIDTFFTRQPPPGTLFAARA
QAEADAVGTVAGGSR
>Mature_575_residues
MLESSAMTPGIRNFCLSALTASLSLAVFAAWGGEIKGVGVSTGATGTRAEIQLAGSGGFKTLSLANPTRLVVDFPESSGV
RGLKLPAAAGLVTSVRTGQPVPGTFRVVFELATPVTPLKPQMQTLGSASTLVIEWPGDPTPAAASAVAAAAPTAAPAPRP
LNAQAEAARATAALAASAQRASSVPPPQPSTPPPAPSVPASAMPTVTQAPVPTTIATGVPTPRPATSATTGAPAPTGVAG
NTPNRAAGAAAAVPSGAVVAGSSAAAAAILNGGSAPMGATSGNAGAIAPNSASGVVAAAGDDDLPPRPVLPSEASRIKMA
PGMRPLVVAIDPGHGGQDPGAMGPTGKREKDVTLAVGRELARQVNATPGMKAYLTRDTDVFIPLPMRAQKARAAKADIFI
SIHADAAENRSATGSSVYVLSTKGASSQRARWLADKENAADLVGGVRLQQTESTLANVLLDLAQSGHMKASEDAAGHVLG
GLKRIGNNHKPQLERANFAVLRTSDMPAMLVETAFISNPDEERRLIDPAYQRRIASAVLDGIDTFFTRQPPPGTLFAARA
QAEADAVGTVAGGSR

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=223, Percent_Identity=46.1883408071749, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI1790611, Length=230, Percent_Identity=46.0869565217391, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1788776, Length=220, Percent_Identity=42.7272727272727, Blast_Score=181, Evalue=9e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 57724; Mature: 57724

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLESSAMTPGIRNFCLSALTASLSLAVFAAWGGEIKGVGVSTGATGTRAEIQLAGSGGFK
CCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCEEEEEEEECCCCCCEEEEEEECCCCEE
TLSLANPTRLVVDFPESSGVRGLKLPAAAGLVTSVRTGQPVPGTFRVVFELATPVTPLKP
EEEECCCEEEEEECCCCCCCCEEECCHHHHHHHHHCCCCCCCCHHEEEEEHHCCCCCCCH
QMQTLGSASTLVIEWPGDPTPAAASAVAAAAPTAAPAPRPLNAQAEAARATAALAASAQR
HHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ASSVPPPQPSTPPPAPSVPASAMPTVTQAPVPTTIATGVPTPRPATSATTGAPAPTGVAG
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCC
NTPNRAAGAAAAVPSGAVVAGSSAAAAAILNGGSAPMGATSGNAGAIAPNSASGVVAAAG
CCCCCCCCCHHCCCCCCEEECCCHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECC
DDDLPPRPVLPSEASRIKMAPGMRPLVVAIDPGHGGQDPGAMGPTGKREKDVTLAVGREL
CCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
ARQVNATPGMKAYLTRDTDVFIPLPMRAQKARAAKADIFISIHADAAENRSATGSSVYVL
HHHHCCCCCCEEEEECCCEEEEECCCCHHHHHCCCEEEEEEEECCCCCCCCCCCCEEEEE
STKGASSQRARWLADKENAADLVGGVRLQQTESTLANVLLDLAQSGHMKASEDAAGHVLG
EECCCCCHHHHHCCCCCCHHHHHCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
GLKRIGNNHKPQLERANFAVLRTSDMPAMLVETAFISNPDEERRLIDPAYQRRIASAVLD
HHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHH
GIDTFFTRQPPPGTLFAARAQAEADAVGTVAGGSR
HHHHHHCCCCCCCCEEEEECCCCCCCEEECCCCCC
>Mature Secondary Structure
MLESSAMTPGIRNFCLSALTASLSLAVFAAWGGEIKGVGVSTGATGTRAEIQLAGSGGFK
CCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCEEEEEEEECCCCCCEEEEEEECCCCEE
TLSLANPTRLVVDFPESSGVRGLKLPAAAGLVTSVRTGQPVPGTFRVVFELATPVTPLKP
EEEECCCEEEEEECCCCCCCCEEECCHHHHHHHHHCCCCCCCCHHEEEEEHHCCCCCCCH
QMQTLGSASTLVIEWPGDPTPAAASAVAAAAPTAAPAPRPLNAQAEAARATAALAASAQR
HHHHCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ASSVPPPQPSTPPPAPSVPASAMPTVTQAPVPTTIATGVPTPRPATSATTGAPAPTGVAG
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCC
NTPNRAAGAAAAVPSGAVVAGSSAAAAAILNGGSAPMGATSGNAGAIAPNSASGVVAAAG
CCCCCCCCCHHCCCCCCEEECCCHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEECC
DDDLPPRPVLPSEASRIKMAPGMRPLVVAIDPGHGGQDPGAMGPTGKREKDVTLAVGREL
CCCCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
ARQVNATPGMKAYLTRDTDVFIPLPMRAQKARAAKADIFISIHADAAENRSATGSSVYVL
HHHHCCCCCCEEEEECCCEEEEECCCCHHHHHCCCEEEEEEEECCCCCCCCCCCCEEEEE
STKGASSQRARWLADKENAADLVGGVRLQQTESTLANVLLDLAQSGHMKASEDAAGHVLG
EECCCCCHHHHHCCCCCCHHHHHCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
GLKRIGNNHKPQLERANFAVLRTSDMPAMLVETAFISNPDEERRLIDPAYQRRIASAVLD
HHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHH
GIDTFFTRQPPPGTLFAARAQAEADAVGTVAGGSR
HHHHHHCCCCCCCCEEEEECCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]