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The map label for this gene is liaR [H]

Identifier: 21242903

GI number: 21242903

Start: 2539741

End: 2540382

Strand: Direct

Name: liaR [H]

Synonym: XAC2168

Alternate gene names: 21242903

Gene position: 2539741-2540382 (Clockwise)

Preceding gene: 21242902

Following gene: 77748634

Centisome position: 49.07

GC content: 67.13

Gene sequence:

>642_bases
ATGGCTGACATGGTCCGACCAACCGACGTGCTGGTGGTAGACGACCATCCGCTGCTGCGCGATGGGCTCAGTGCCATGCT
CGCCGCCGAGCACGACATGCGCGTGGTCGGCGAAGCGGAAGACGGCGAACAGGCGGTGGCCTGCTACACGCGCCTGCGCC
CGGACGTGGTGCTGATGGATCTGCAGATGCCGCGCGTGGACGGGGTCGAAGCCATCCAGCGCATCCGCCAGGTCGACTCC
GCCGCCAAGGTCATCGTGCTGACCACCTACACCGGCGACGTGCGCGCGGTACGCGCCCTCCAGGCAGGCGCCTGCGGTTA
CCTGCTCAAGAGCGCGTTGCGCCGTGAGCTGGTCGACACCATCCGCGACGTGCGCCGCGGCCAGCGCCGGCATGTGCCGG
CCTCGGTGGCCGAAAATATCGCCGCCCATGTGCTCGATGATGCCCTGTCCGCCCGCGAGACCGAAGTGCTGAACCTGGTC
GCTACCGGCTGCTCCAACAAGCAGATCGGCAGTGCCTTGAGCATCTCCGAAGAAACCGTCAAGGCACATATGAAGAACAT
CCTGGGCAAGCTCGGCGTGCGCGATCGCACCCATGCGGTCACCGTCGCGCTGCGGCGCGGGATCCTGTCGCTGGAGACCT
GA

Upstream 100 bases:

>100_bases
GGCACCGAAATCCAGATCACCCTGCCCGCGCGTGCCGCCTACCTGCGCGCCAAGCGATGGTGGTGGCCATTTCACACCGC
ACGATCCACGGAGACTTCCA

Downstream 100 bases:

>100_bases
GCGGCGGCTTGCCACTCCTGCGCGCCACCAAGGGGGTGGCCTTTGCTATACGGTCGACCATTCTTGAACGCGAGGCGACA
CGCGCGATAGCCGCGCGCGC

Product: two-component system regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MADMVRPTDVLVVDDHPLLRDGLSAMLAAEHDMRVVGEAEDGEQAVACYTRLRPDVVLMDLQMPRVDGVEAIQRIRQVDS
AAKVIVLTTYTGDVRAVRALQAGACGYLLKSALRRELVDTIRDVRRGQRRHVPASVAENIAAHVLDDALSARETEVLNLV
ATGCSNKQIGSALSISEETVKAHMKNILGKLGVRDRTHAVTVALRRGILSLET

Sequences:

>Translated_213_residues
MADMVRPTDVLVVDDHPLLRDGLSAMLAAEHDMRVVGEAEDGEQAVACYTRLRPDVVLMDLQMPRVDGVEAIQRIRQVDS
AAKVIVLTTYTGDVRAVRALQAGACGYLLKSALRRELVDTIRDVRRGQRRHVPASVAENIAAHVLDDALSARETEVLNLV
ATGCSNKQIGSALSISEETVKAHMKNILGKLGVRDRTHAVTVALRRGILSLET
>Mature_212_residues
ADMVRPTDVLVVDDHPLLRDGLSAMLAAEHDMRVVGEAEDGEQAVACYTRLRPDVVLMDLQMPRVDGVEAIQRIRQVDSA
AKVIVLTTYTGDVRAVRALQAGACGYLLKSALRRELVDTIRDVRRGQRRHVPASVAENIAAHVLDDALSARETEVLNLVA
TGCSNKQIGSALSISEETVKAHMKNILGKLGVRDRTHAVTVALRRGILSLET

Specific function: Member of the two-component regulatory system liaS/liaR probably involved in response to a subset of cell wall-active antibiotics that interfere with the lipid II cycle in the cytoplasmic membrane (bacitracin, nisin, ramoplanin and vancomycin). Seems also

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=206, Percent_Identity=37.378640776699, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1788222, Length=213, Percent_Identity=33.8028169014084, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1786747, Length=196, Percent_Identity=31.1224489795918, Blast_Score=112, Evalue=1e-26,
Organism=Escherichia coli, GI1787473, Length=186, Percent_Identity=34.9462365591398, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790102, Length=186, Percent_Identity=31.1827956989247, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788712, Length=204, Percent_Identity=24.0196078431373, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87082052, Length=133, Percent_Identity=31.5789473684211, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788546, Length=194, Percent_Identity=29.8969072164948, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788550, Length=101, Percent_Identity=35.6435643564356, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23193; Mature: 23062

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADMVRPTDVLVVDDHPLLRDGLSAMLAAEHDMRVVGEAEDGEQAVACYTRLRPDVVLMD
CCCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCEEEEE
LQMPRVDGVEAIQRIRQVDSAAKVIVLTTYTGDVRAVRALQAGACGYLLKSALRRELVDT
CCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
IRDVRRGQRRHVPASVAENIAAHVLDDALSARETEVLNLVATGCSNKQIGSALSISEETV
HHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
KAHMKNILGKLGVRDRTHAVTVALRRGILSLET
HHHHHHHHHHCCCCCHHHHHHHHHHHCHHHCCC
>Mature Secondary Structure 
ADMVRPTDVLVVDDHPLLRDGLSAMLAAEHDMRVVGEAEDGEQAVACYTRLRPDVVLMD
CCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHCCCCEEEEE
LQMPRVDGVEAIQRIRQVDSAAKVIVLTTYTGDVRAVRALQAGACGYLLKSALRRELVDT
CCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHH
IRDVRRGQRRHVPASVAENIAAHVLDDALSARETEVLNLVATGCSNKQIGSALSISEETV
HHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
KAHMKNILGKLGVRDRTHAVTVALRRGILSLET
HHHHHHHHHHCCCCCHHHHHHHHHHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9639930; 9384377 [H]