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The map label for this gene is afr [H]

Identifier: 21242872

GI number: 21242872

Start: 2491710

End: 2492396

Strand: Reverse

Name: afr [H]

Synonym: XAC2137

Alternate gene names: 21242872

Gene position: 2492396-2491710 (Counterclockwise)

Preceding gene: 21242873

Following gene: 21242871

Centisome position: 48.16

GC content: 63.32

Gene sequence:

>687_bases
TTGCGCTGGGGTGTGCTGGGGCCTGGCAAGATCGCATCCGCATTCGTCGATGCCTTGCGTCGCAATACCCGCCAATGTCC
ATTCGCGGTAGCCTCGCGCAGCCGCGAACGTGCGCAGACGTTCGCCGACACCTGGGCGATGGAACACGCTTACGACAGCT
ACGAGGCGCTCGTCCGCCACCCGGACGTCGACATCGTCTACATTGCGACCCCACACAGCGAACATCTGGAACATGGCCTG
CTCGCACTTCGCGCGGGCAAGCATGTCCTGATCGAAAAACCCATGACCACCTGCGCGCAGGATGCCCGCATCCTGGTGCA
GGAAGCGCGCGCGCGGACTGTTCCTGATGGAGGCCATGTGGAGCCGTTATCTTCCACACATCTCGGTAGTGCGCAAGCTG
CTGGCCGATGGAGCGTTGGGCGAAGTCCGGCACGTGTTCGCAGACCTGAGTCAATCGGGGCCTCGCGATCCATTGCATCG
GCAACGCAACGCAGCACTGGGTGGCGGTGCGTTACTCGACCTGGGCGTCTACACCGTGCAGTTTTCCTCAATGGTGTTGG
GCGCGCCCAGCGCAATCACGGCCGTGGGCGCGCTCACCGAAACCGGTGTCGATGCCTATTCGACCGTGGTGCTGTCGCAT
GGCGGGCACGCGCAATCCACCCTGATCAGTTCCATCGTGGCCCGTAG

Upstream 100 bases:

>100_bases
TTGCTAGCATCGCAACATCACCGATGCAGAGCACATCATCATGCTGCCGACGACATTCCCGACGCCGGATTTGTTCTTGC
CAGGCCACGGCGAACCTGTA

Downstream 100 bases:

>100_bases
CAGCTCCACCGCCTATGTCACCGGTAGCGCTGGCCGTATCGATCTGGCAGGCGATTTCCATAACCCGACCACGCTACGTC
TGGTCGGCAACGACTACGCC

Product: oxidoreductase

Products: NA

Alternate protein names: Anhydrofructose reductase; 1,5-anhydro-D-fructose reductase (1,5-anhydro-D-mannitol-forming) [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MRWGVLGPGKIASAFVDALRRNTRQCPFAVASRSRERAQTFADTWAMEHAYDSYEALVRHPDVDIVYIATPHSEHLEHGL
LALRAGKHVLIEKPMTTCAQDARILVQEARARTVPDGGHVEPLSSTHLGSAQAAGRWSVGRSPARVRRPESIGASRSIAS
ATQRSTGWRCVTRPGRLHRAVFLNGVGRAQRNHGRGRAHRNRCRCLFDRGAVAWRARAIHPDQFHRGP

Sequences:

>Translated_228_residues
MRWGVLGPGKIASAFVDALRRNTRQCPFAVASRSRERAQTFADTWAMEHAYDSYEALVRHPDVDIVYIATPHSEHLEHGL
LALRAGKHVLIEKPMTTCAQDARILVQEARARTVPDGGHVEPLSSTHLGSAQAAGRWSVGRSPARVRRPESIGASRSIAS
ATQRSTGWRCVTRPGRLHRAVFLNGVGRAQRNHGRGRAHRNRCRCLFDRGAVAWRARAIHPDQFHRGP
>Mature_228_residues
MRWGVLGPGKIASAFVDALRRNTRQCPFAVASRSRERAQTFADTWAMEHAYDSYEALVRHPDVDIVYIATPHSEHLEHGL
LALRAGKHVLIEKPMTTCAQDARILVQEARARTVPDGGHVEPLSSTHLGSAQAAGRWSVGRSPARVRRPESIGASRSIAS
ATQRSTGWRCVTRPGRLHRAVFLNGVGRAQRNHGRGRAHRNRCRCLFDRGAVAWRARAIHPDQFHRGP

Specific function: Catalyzes the NADPH-specific reduction of 1,5-anhydro-D- fructose to 1,5-anhydro-D-mannitol. Also shows some activity against structurally related compounds such as 3-keto-1,5-anhydro- D-fructose, D-glucosone and D-xylosone. The enzyme cannot use NADH as

COG id: COG0673

COG function: function code R; Predicted dehydrogenases and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfo/idh/mocA family [H]

Homologues:

Organism=Homo sapiens, GI7657212, Length=117, Percent_Identity=41.025641025641, Blast_Score=81, Evalue=7e-16,
Organism=Escherichia coli, GI145693182, Length=119, Percent_Identity=33.6134453781513, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581117, Length=114, Percent_Identity=40.3508771929825, Blast_Score=81, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000683
- InterPro:   IPR004104 [H]

Pfam domain/function: PF01408 GFO_IDH_MocA; PF02894 GFO_IDH_MocA_C [H]

EC number: =1.1.1.292 [H]

Molecular weight: Translated: 25335; Mature: 25335

Theoretical pI: Translated: 11.93; Mature: 11.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRWGVLGPGKIASAFVDALRRNTRQCPFAVASRSRERAQTFADTWAMEHAYDSYEALVRH
CCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PDVDIVYIATPHSEHLEHGLLALRAGKHVLIEKPMTTCAQDARILVQEARARTVPDGGHV
CCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCC
EPLSSTHLGSAQAAGRWSVGRSPARVRRPESIGASRSIASATQRSTGWRCVTRPGRLHRA
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHCCCHHHHHHHHHHCCCCEEECCCCHHHHH
VFLNGVGRAQRNHGRGRAHRNRCRCLFDRGAVAWRARAIHPDQFHRGP
HHHHCCCHHHHCCCCCCCHHHHHHHHHCCCCCEEHEECCCHHHCCCCC
>Mature Secondary Structure
MRWGVLGPGKIASAFVDALRRNTRQCPFAVASRSRERAQTFADTWAMEHAYDSYEALVRH
CCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PDVDIVYIATPHSEHLEHGLLALRAGKHVLIEKPMTTCAQDARILVQEARARTVPDGGHV
CCCCEEEEECCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCC
EPLSSTHLGSAQAAGRWSVGRSPARVRRPESIGASRSIASATQRSTGWRCVTRPGRLHRA
CCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHCCCHHHHHHHHHHCCCCEEECCCCHHHHH
VFLNGVGRAQRNHGRGRAHRNRCRCLFDRGAVAWRARAIHPDQFHRGP
HHHHCCCHHHHCCCCCCCHHHHHHHHHCCCCCEEHEECCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA