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The map label for this gene is gacA [H]

Identifier: 21242845

GI number: 21242845

Start: 2463557

End: 2464204

Strand: Direct

Name: gacA [H]

Synonym: XAC2110

Alternate gene names: 21242845

Gene position: 2463557-2464204 (Clockwise)

Preceding gene: 21242844

Following gene: 21242847

Centisome position: 47.6

GC content: 60.19

Gene sequence:

>648_bases
ATGACCATCAGAGTTTTTCTGATCGACGATCATGCACTCGTGCGTACAGGCATGAAGATGATTCTGTCCAAGGAAGTGGA
TGTCGATGTGGTGGGTGAAGCAGAGAGCGGCGAAGCTGCATTGCCGCAGATTCGTCAACTCAAACCGGACATCGTGCTGT
GCGACCTGCATTTACCCGGCGTCAGCGGCCTGGAAATTACCGAGCGCATCGTCAAGGGCGACTACGGCACGCGGGTCATC
ATCGTCTCCGTGCTCGAAGACGGCCCATTGCCCAAGCGTTTGCTCGAGGCAGGCGCCTCCGGTTATGTGGGCAAGGGCGG
TGACGCGCACGAGTTGCTGCGCGCGGTGCGTGAAGTCGCATTGGGACGGCGTTACCTCGGCAACACGATCGCGCAGAACC
TGGCTCTGTCCAATCTGGAAGGTGGCGGTTCACCGTTCGATGCATTGTCGCCCCGCGAGCTGGAAGTGGCGTTGCTGCTG
ACCCAAGGGCTGCGCCAGGAAGACATCGCCAAGCGCCTGAACCTCAGCGCCAAGACCATCAACACGCACAAGGCACGCCT
GTTTGAGAAGGTCGGCATCCAGGACAACATCGCGCTGGCGCGGCTGGCCAATCAATACGGTCTGACCGATCCCTCCCGGG
TGCTGTGA

Upstream 100 bases:

>100_bases
CATAACGCTCTAACTTGGGCGTAATACGTCGCATCGAGCTTGCCCTGAATTATTCACGGAGATAGGCTGCACCAGCCAGC
CGCTAGCGAGGAAAACAAGG

Downstream 100 bases:

>100_bases
ACCGTTACCTTGGGTGCGCCGCACTGGCTGCGCTGCATCGCAAGGTCGGGGCGAACAACATGGCAGAGAGAGGGCTCATA
GCGTGCTCAGAAGCGCGCTT

Product: LuxR/UhpA family transcriptional regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MTIRVFLIDDHALVRTGMKMILSKEVDVDVVGEAESGEAALPQIRQLKPDIVLCDLHLPGVSGLEITERIVKGDYGTRVI
IVSVLEDGPLPKRLLEAGASGYVGKGGDAHELLRAVREVALGRRYLGNTIAQNLALSNLEGGGSPFDALSPRELEVALLL
TQGLRQEDIAKRLNLSAKTINTHKARLFEKVGIQDNIALARLANQYGLTDPSRVL

Sequences:

>Translated_215_residues
MTIRVFLIDDHALVRTGMKMILSKEVDVDVVGEAESGEAALPQIRQLKPDIVLCDLHLPGVSGLEITERIVKGDYGTRVI
IVSVLEDGPLPKRLLEAGASGYVGKGGDAHELLRAVREVALGRRYLGNTIAQNLALSNLEGGGSPFDALSPRELEVALLL
TQGLRQEDIAKRLNLSAKTINTHKARLFEKVGIQDNIALARLANQYGLTDPSRVL
>Mature_214_residues
TIRVFLIDDHALVRTGMKMILSKEVDVDVVGEAESGEAALPQIRQLKPDIVLCDLHLPGVSGLEITERIVKGDYGTRVII
VSVLEDGPLPKRLLEAGASGYVGKGGDAHELLRAVREVALGRRYLGNTIAQNLALSNLEGGGSPFDALSPRELEVALLLT
QGLRQEDIAKRLNLSAKTINTHKARLFEKVGIQDNIALARLANQYGLTDPSRVL

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=210, Percent_Identity=40.9523809523809, Blast_Score=177, Evalue=4e-46,
Organism=Escherichia coli, GI1790102, Length=199, Percent_Identity=38.6934673366834, Blast_Score=118, Evalue=4e-28,
Organism=Escherichia coli, GI1788712, Length=189, Percent_Identity=31.7460317460317, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788521, Length=197, Percent_Identity=32.48730964467, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1786747, Length=204, Percent_Identity=25.4901960784314, Blast_Score=97, Evalue=6e-22,
Organism=Escherichia coli, GI1787473, Length=182, Percent_Identity=34.0659340659341, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788546, Length=202, Percent_Identity=29.2079207920792, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23276; Mature: 23145

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRVFLIDDHALVRTGMKMILSKEVDVDVVGEAESGEAALPQIRQLKPDIVLCDLHLPG
CEEEEEEECCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHCCCCEEEEEEECCC
VSGLEITERIVKGDYGTRVIIVSVLEDGPLPKRLLEAGASGYVGKGGDAHELLRAVREVA
CCCHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LGRRYLGNTIAQNLALSNLEGGGSPFDALSPRELEVALLLTQGLRQEDIAKRLNLSAKTI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHH
NTHKARLFEKVGIQDNIALARLANQYGLTDPSRVL
HHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure 
TIRVFLIDDHALVRTGMKMILSKEVDVDVVGEAESGEAALPQIRQLKPDIVLCDLHLPG
EEEEEEECCHHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHCCCCEEEEEEECCC
VSGLEITERIVKGDYGTRVIIVSVLEDGPLPKRLLEAGASGYVGKGGDAHELLRAVREVA
CCCHHHHHHHHCCCCCCEEEEEEEECCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LGRRYLGNTIAQNLALSNLEGGGSPFDALSPRELEVALLLTQGLRQEDIAKRLNLSAKTI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHH
NTHKARLFEKVGIQDNIALARLANQYGLTDPSRVL
HHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]