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The map label for this gene is eda [H]

Identifier: 21242806

GI number: 21242806

Start: 2414905

End: 2415564

Strand: Reverse

Name: eda [H]

Synonym: XAC2067

Alternate gene names: 21242806

Gene position: 2415564-2414905 (Counterclockwise)

Preceding gene: 21242807

Following gene: 21242802

Centisome position: 46.67

GC content: 68.94

Gene sequence:

>660_bases
ATGACGATTGCCCAGACCCAGAACACCGCCGAACAGTTGCTGCGCGATGCCGGCATCCTGCCGGTGGTCACCGTGGACAC
GCTGGACCAGGCGCGCCGCGTCGCCGATGCGCTGCTCGAAGGCGGCCTGCCCGCGATCGAGCTGACCCTGCGCACGCCGG
TTGCGATCGACGCGCTGGCGATGCTCAAGCGCGAGTTGCCCAACATCGTGATCGGCGCGGGCACCGTGCTGAGCGAATTG
CAGCTGCGTCAGTCGGTGGATGCCGGTGCAGACTTCCTGGTGACCCCGGGCACGCCCGCGCCGCTGGCGCGCCTGCTGGC
CGACGCACCGATTCCCGCCGTACCTGGCGCAGCAACGCCGACCGAGCTGCTGACCCTGATGGGCCTGGGATTCCGCGTCT
GCAAGCTGTTCCCGGCCACCGCCGTGGGCGGTCTGCAGATGCTCAAGGGCCTGGCTGGCCCGTTGTCCGAGCTCAAACTG
TGCCCCACCGGCGGCATCAGCGAGTCCAACGCGGCCGAGTTCCTGTCGCAGCCGAATGTGCTGTGCATCGGCGGTTCGTG
GATGGTCCCCAAGGACTGGCTGGCGCAGGGCCAATGGGACAAGGTCAAGGAAAGTTCGGCCAAGGCGGCGGCGATCGTGC
GGCAGGTGCGGGCGGGCTGA

Upstream 100 bases:

>100_bases
GCCGATGCCGCAGCCGCCGCGGCACCGCACGAGTCCAAGGACGCCTGATCCCCTTCCCTCTCTGCGACCGCTCCGTTGGT
CGCCGTCACCCGGAGTTGCA

Downstream 100 bases:

>100_bases
TTGGCTGGGCGCCATATCCGGCAGGTCGATCGTTGGATGGCTTCGAATCTGCATGTCGATACAGATGTGCTGATTCGCCG
ATGACGGCATGAAGATCGAG

Product: keto-hydroxyglutarate-aldolase/keto-deoxy- phosphogluconate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MTIAQTQNTAEQLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIDALAMLKRELPNIVIGAGTVLSEL
QLRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATPTELLTLMGLGFRVCKLFPATAVGGLQMLKGLAGPLSELKL
CPTGGISESNAAEFLSQPNVLCIGGSWMVPKDWLAQGQWDKVKESSAKAAAIVRQVRAG

Sequences:

>Translated_219_residues
MTIAQTQNTAEQLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIDALAMLKRELPNIVIGAGTVLSEL
QLRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATPTELLTLMGLGFRVCKLFPATAVGGLQMLKGLAGPLSELKL
CPTGGISESNAAEFLSQPNVLCIGGSWMVPKDWLAQGQWDKVKESSAKAAAIVRQVRAG
>Mature_218_residues
TIAQTQNTAEQLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIDALAMLKRELPNIVIGAGTVLSELQ
LRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATPTELLTLMGLGFRVCKLFPATAVGGLQMLKGLAGPLSELKLC
PTGGISESNAAEFLSQPNVLCIGGSWMVPKDWLAQGQWDKVKESSAKAAAIVRQVRAG

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1788156, Length=203, Percent_Identity=41.3793103448276, Blast_Score=165, Evalue=3e-42,
Organism=Escherichia coli, GI48994954, Length=200, Percent_Identity=29, Blast_Score=64, Evalue=6e-12,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 22776; Mature: 22645

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00159 ALDOLASE_KDPG_KHG_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAQTQNTAEQLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIDALA
CCCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
MLKRELPNIVIGAGTVLSELQLRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATP
HHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCCH
TELLTLMGLGFRVCKLFPATAVGGLQMLKGLAGPLSELKLCPTGGISESNAAEFLSQPNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCEECCCCCCCCCHHHHHHCCCCE
LCIGGSWMVPKDWLAQGQWDKVKESSAKAAAIVRQVRAG
EEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIAQTQNTAEQLLRDAGILPVVTVDTLDQARRVADALLEGGLPAIELTLRTPVAIDALA
CCCCCHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHH
MLKRELPNIVIGAGTVLSELQLRQSVDAGADFLVTPGTPAPLARLLADAPIPAVPGAATP
HHHHHCCCEEEEHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCCH
TELLTLMGLGFRVCKLFPATAVGGLQMLKGLAGPLSELKLCPTGGISESNAAEFLSQPNV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCEECCCCCCCCCHHHHHHCCCCE
LCIGGSWMVPKDWLAQGQWDKVKESSAKAAAIVRQVRAG
EEECCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8145647 [H]