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The map label for this gene is xthA2 [H]

Identifier: 21242774

GI number: 21242774

Start: 2375121

End: 2376113

Strand: Direct

Name: xthA2 [H]

Synonym: XAC2030

Alternate gene names: 21242774

Gene position: 2375121-2376113 (Clockwise)

Preceding gene: 21242772

Following gene: 21242776

Centisome position: 45.89

GC content: 62.54

Gene sequence:

>993_bases
ATGTCAGGCACCTCACGTAAGATCGCGACGTTCAACGTCAATGGTATCGCCAGCCGTCTGCCGCACCTGCTGGAATGGTT
GCAACGCGATCAGCCGGACATCGTGGGCCTGCAGGAATTAAAGTCGACACAGGTTGCGTTTCCTGAGCAGGCGATTCGCG
ATGCCGGGTACGGTGTGATCTGGCAGGGCGAAAAGTCCTGGAATGGCGTGGCACTGCTTGCGCGGGGTATCGAGCCAGTG
GAGATTCGCCGCGGGTTGCCCTGGGATCCGCGCGACACGCAAAGCCGCTATCTGGAGGCGGCGATTCATGGCGTGGTGGT
GGCCTGCCTGTATTTGCCCAACGGCAACCCGCAGCCCGGGCCGAAGTTCGACTACAAGCTTGCATGGTTCGAGCGCCTGA
TCCGGCACGCGAAAACGCTGGTGGACCTGCCGCATCCCGTGGCGCTGATCGGCGACTTCAATGTGGTGCCGACCGATGCG
GATATCTACGACCCCAAGGGCTGGCGCAAGGATGCCCTGCTGCAGCCGGAAAGCCGTGCGGCCTACCAGACCCTGCTAGC
GCAGGGGTGGACCGATAGCTTGCTGGCAATCCACGGCCAGACGCCGATCTATACATTCTGGGATTACTTCCGTCAGCACT
TTGCGCGCGACCGTGGGTTGCGCATCGATCATCTGCTGCTCAATCGCACGCTGGCGCCCGGCTTGCAAGATGCCGGCGTC
GACAAGTGGGTACGTGCGCTGGAGAAGGCCAGCGATCATGCACCGACCTGGATCAGCGTGCGCGTGCCGGATGCGCCCGC
TGAAGCGGCGCCGGATACCACGCCTATGGCGAAGGTGCGCAAGCCGGGCGCAGCGAAAAAGGCGGCCGTGCCCAAGGCGG
CGGCCAAGAAGACGTCCAGTGTCACTGCAAAAAAAGCGGTGAAGAAGATCGTCGCAAAGAGGGCCACAGCCGGATCGGCG
ACACCACGCAAGTCGGCGAAAAAGAACAGCTGA

Upstream 100 bases:

>100_bases
GCGGGCCGTGTCACGCAGTGCAGCTTCACCTCGCTTGAGCATCGGCTTCATCAGGCGGCGGTCATGCTGTTGCACCACGG
AAGCGGCCCCTTGCATGCCC

Downstream 100 bases:

>100_bases
CTAGCTCACCGGCCGGCCGGAGTCGACGCAGCCTATCTACGCTGCGCGTTCGAGCTTGCGGCGCTTGACCCGCTGCAATG
CGTAGCCGGTCAGGGCCGTC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSGTSRKIATFNVNGIASRLPHLLEWLQRDQPDIVGLQELKSTQVAFPEQAIRDAGYGVIWQGEKSWNGVALLARGIEPV
EIRRGLPWDPRDTQSRYLEAAIHGVVVACLYLPNGNPQPGPKFDYKLAWFERLIRHAKTLVDLPHPVALIGDFNVVPTDA
DIYDPKGWRKDALLQPESRAAYQTLLAQGWTDSLLAIHGQTPIYTFWDYFRQHFARDRGLRIDHLLLNRTLAPGLQDAGV
DKWVRALEKASDHAPTWISVRVPDAPAEAAPDTTPMAKVRKPGAAKKAAVPKAAAKKTSSVTAKKAVKKIVAKRATAGSA
TPRKSAKKNS

Sequences:

>Translated_330_residues
MSGTSRKIATFNVNGIASRLPHLLEWLQRDQPDIVGLQELKSTQVAFPEQAIRDAGYGVIWQGEKSWNGVALLARGIEPV
EIRRGLPWDPRDTQSRYLEAAIHGVVVACLYLPNGNPQPGPKFDYKLAWFERLIRHAKTLVDLPHPVALIGDFNVVPTDA
DIYDPKGWRKDALLQPESRAAYQTLLAQGWTDSLLAIHGQTPIYTFWDYFRQHFARDRGLRIDHLLLNRTLAPGLQDAGV
DKWVRALEKASDHAPTWISVRVPDAPAEAAPDTTPMAKVRKPGAAKKAAVPKAAAKKTSSVTAKKAVKKIVAKRATAGSA
TPRKSAKKNS
>Mature_329_residues
SGTSRKIATFNVNGIASRLPHLLEWLQRDQPDIVGLQELKSTQVAFPEQAIRDAGYGVIWQGEKSWNGVALLARGIEPVE
IRRGLPWDPRDTQSRYLEAAIHGVVVACLYLPNGNPQPGPKFDYKLAWFERLIRHAKTLVDLPHPVALIGDFNVVPTDAD
IYDPKGWRKDALLQPESRAAYQTLLAQGWTDSLLAIHGQTPIYTFWDYFRQHFARDRGLRIDHLLLNRTLAPGLQDAGVD
KWVRALEKASDHAPTWISVRVPDAPAEAAPDTTPMAKVRKPGAAKKAAVPKAAAKKTSSVTAKKAVKKIVAKRATAGSAT
PRKSAKKNS

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=256, Percent_Identity=29.6875, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI18375503, Length=256, Percent_Identity=29.6875, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI18375501, Length=256, Percent_Identity=29.6875, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1788046, Length=268, Percent_Identity=36.1940298507463, Blast_Score=153, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI71989536, Length=237, Percent_Identity=24.8945147679325, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI221330655, Length=258, Percent_Identity=28.6821705426357, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI17136678, Length=258, Percent_Identity=28.6821705426357, Blast_Score=98, Evalue=8e-21,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 36432; Mature: 36301

Theoretical pI: Translated: 10.48; Mature: 10.48

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTSRKIATFNVNGIASRLPHLLEWLQRDQPDIVGLQELKSTQVAFPEQAIRDAGYGVI
CCCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHCCCHHHHHHCCCCEE
WQGEKSWNGVALLARGIEPVEIRRGLPWDPRDTQSRYLEAAIHGVVVACLYLPNGNPQPG
ECCCCCCCCHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCC
PKFDYKLAWFERLIRHAKTLVDLPHPVALIGDFNVVPTDADIYDPKGWRKDALLQPESRA
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCEECCCCCCCCCCCCCCCCCCCCCHHHH
AYQTLLAQGWTDSLLAIHGQTPIYTFWDYFRQHFARDRGLRIDHLLLNRTLAPGLQDAGV
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCH
DKWVRALEKASDHAPTWISVRVPDAPAEAAPDTTPMAKVRKPGAAKKAAVPKAAAKKTSS
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCCHHHHCCHHHHHHHHH
VTAKKAVKKIVAKRATAGSATPRKSAKKNS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SGTSRKIATFNVNGIASRLPHLLEWLQRDQPDIVGLQELKSTQVAFPEQAIRDAGYGVI
CCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCEECHHHHHHHHHCCCHHHHHHCCCCEE
WQGEKSWNGVALLARGIEPVEIRRGLPWDPRDTQSRYLEAAIHGVVVACLYLPNGNPQPG
ECCCCCCCCHHHHHCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCC
PKFDYKLAWFERLIRHAKTLVDLPHPVALIGDFNVVPTDADIYDPKGWRKDALLQPESRA
CCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCEECCCCCCCCCCCCCCCCCCCCCHHHH
AYQTLLAQGWTDSLLAIHGQTPIYTFWDYFRQHFARDRGLRIDHLLLNRTLAPGLQDAGV
HHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCH
DKWVRALEKASDHAPTWISVRVPDAPAEAAPDTTPMAKVRKPGAAKKAAVPKAAAKKTSS
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCCHHHHCCHHHHHHHHH
VTAKKAVKKIVAKRATAGSATPRKSAKKNS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]