Click here to switch to the map view.

The map label for this gene is rlmN [H]

Identifier: 21242760

GI number: 21242760

Start: 2358898

End: 2360115

Strand: Direct

Name: rlmN [H]

Synonym: XAC2016

Alternate gene names: 21242760

Gene position: 2358898-2360115 (Clockwise)

Preceding gene: 21242759

Following gene: 21242761

Centisome position: 45.58

GC content: 62.81

Gene sequence:

>1218_bases
GTGAGTCGAATCGTGAATGAGGTCGTGATCCCTTCCGTGCTGCAGGACGGCTCCGTCCGTACCCCGGAGCCGCGCAAGCA
GAACCTGCTCGACCTCGATCGCGAGGGGCTGGAGCGCTTCTTCGCCGATACGCTTGGCGAGGCGCGTTACCGTGCCCATC
AGGTGATGAAGTGGATCCATCATCGCTACGTCACCGATTTCGACCAGATGACGGACCTCGGCAAGGCGCTGCGTGCGAAG
TTGCACCAACACGCCGAGGTCCTCGTCCCGAACGTGGTGTTCGACAAGCCTTCGGCCGACGGCACCCACAAATGGCTGTT
GGCCATGGGCACCGACGGCAAGAACGCCATCGAAACCGTGTACATCCCCGACAAGGGCCGTGGCACGCTGTGCGTGTCCT
CCCAGGTCGGCTGCGGCTTGAACTGCAGCTTTTGTTCGACCGCTACCCAGGGCTTCAACCGCAACCTCACCACGGCCGAA
ATCATCGGCCAGGTGTGGGTGGCGGCGCGCCATCTGGGCAATGTGCCGCACCAGCAGCGCCGTCTCACCAATGTGGTGAT
GATGGGCATGGGCGAGCCGCTGATGAATTTCGACAACGTCGTGCGCGCGATGAGCGTAATGCGCGACGATCTTGGCTACG
GGCTGGCCAGCAAGCGGGTCACGCTGTCGACCTCCGGCCTGGTGCCGATGATCGACCGGCTCTCCACCGAAAGCGACGTC
TCGCTGGCGGTCTCGCTGCATGCAGCCAACGATGCCCTGCGGGAAAGCCTGGTGCCGTTGAACAAGAAATACCCGATCGC
CGAGTTGATGGAGTCGTGCGCACGCTACTTGCGCGGCAGCAAGAAACGCGATTCGGTGACATTCGAATACACGCTGATGA
AAGGCATCAACGACCAGCCGGAGCATGCGCGCCAGTTGGCCAGGTTGATGCGGCAGTTCGACAACGCCGTGCAGTCCAAG
GACGCCGGCAAGGTCAATCTGATTCCGTTCAATCCGTTTCCTGGGACGCGCTATGAGCGCTCGGGCGAGACCGAGATTCG
CGCGTTCCAGAAGATCCTGCTCGATGCGCAGGTGTTGACGATGGTTCGCCGCACCCGTGGCGACGACATCGACGCCGCAT
GCGGGCAGCTCAAGGGGCAGGTCATGGACCGTACCCGTCGCCAGGCGGAGTTCCGCCGCACATTGGAAGGGCAGGCCGAT
CGAGATGCGGCAGCGTGA

Upstream 100 bases:

>100_bases
CGATGCCAATGCTGCCCACGGCTCGGATTCGGTCGAGAACGCTGCCAACGAAGTCGCGTATTTCTTTGCCGCCACCGAAG
TGGTCTCGCGCTGAGGCCGA

Downstream 100 bases:

>100_bases
ACTCGCGTTAATGGCGGCCGTGGCGATCGCGCTGAGCGCGGTCGGTTGCGGTGGTCGCAATGCCAAGGCCGGCTCGGCCA
AGAGTGTGGAGGAAGTCGCG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MSRIVNEVVIPSVLQDGSVRTPEPRKQNLLDLDREGLERFFADTLGEARYRAHQVMKWIHHRYVTDFDQMTDLGKALRAK
LHQHAEVLVPNVVFDKPSADGTHKWLLAMGTDGKNAIETVYIPDKGRGTLCVSSQVGCGLNCSFCSTATQGFNRNLTTAE
IIGQVWVAARHLGNVPHQQRRLTNVVMMGMGEPLMNFDNVVRAMSVMRDDLGYGLASKRVTLSTSGLVPMIDRLSTESDV
SLAVSLHAANDALRESLVPLNKKYPIAELMESCARYLRGSKKRDSVTFEYTLMKGINDQPEHARQLARLMRQFDNAVQSK
DAGKVNLIPFNPFPGTRYERSGETEIRAFQKILLDAQVLTMVRRTRGDDIDAACGQLKGQVMDRTRRQAEFRRTLEGQAD
RDAAA

Sequences:

>Translated_405_residues
MSRIVNEVVIPSVLQDGSVRTPEPRKQNLLDLDREGLERFFADTLGEARYRAHQVMKWIHHRYVTDFDQMTDLGKALRAK
LHQHAEVLVPNVVFDKPSADGTHKWLLAMGTDGKNAIETVYIPDKGRGTLCVSSQVGCGLNCSFCSTATQGFNRNLTTAE
IIGQVWVAARHLGNVPHQQRRLTNVVMMGMGEPLMNFDNVVRAMSVMRDDLGYGLASKRVTLSTSGLVPMIDRLSTESDV
SLAVSLHAANDALRESLVPLNKKYPIAELMESCARYLRGSKKRDSVTFEYTLMKGINDQPEHARQLARLMRQFDNAVQSK
DAGKVNLIPFNPFPGTRYERSGETEIRAFQKILLDAQVLTMVRRTRGDDIDAACGQLKGQVMDRTRRQAEFRRTLEGQAD
RDAAA
>Mature_404_residues
SRIVNEVVIPSVLQDGSVRTPEPRKQNLLDLDREGLERFFADTLGEARYRAHQVMKWIHHRYVTDFDQMTDLGKALRAKL
HQHAEVLVPNVVFDKPSADGTHKWLLAMGTDGKNAIETVYIPDKGRGTLCVSSQVGCGLNCSFCSTATQGFNRNLTTAEI
IGQVWVAARHLGNVPHQQRRLTNVVMMGMGEPLMNFDNVVRAMSVMRDDLGYGLASKRVTLSTSGLVPMIDRLSTESDVS
LAVSLHAANDALRESLVPLNKKYPIAELMESCARYLRGSKKRDSVTFEYTLMKGINDQPEHARQLARLMRQFDNAVQSKD
AGKVNLIPFNPFPGTRYERSGETEIRAFQKILLDAQVLTMVRRTRGDDIDAACGQLKGQVMDRTRRQAEFRRTLEGQADR
DAAA

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=376, Percent_Identity=53.1914893617021, Blast_Score=397, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 45361; Mature: 45229

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIVNEVVIPSVLQDGSVRTPEPRKQNLLDLDREGLERFFADTLGEARYRAHQVMKWIH
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HRYVTDFDQMTDLGKALRAKLHQHAEVLVPNVVFDKPSADGTHKWLLAMGTDGKNAIETV
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEEECCCCCCCEEEE
YIPDKGRGTLCVSSQVGCGLNCSFCSTATQGFNRNLTTAEIIGQVWVAARHLGNVPHQQR
EECCCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
RLTNVVMMGMGEPLMNFDNVVRAMSVMRDDLGYGLASKRVTLSTSGLVPMIDRLSTESDV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCCCCC
SLAVSLHAANDALRESLVPLNKKYPIAELMESCARYLRGSKKRDSVTFEYTLMKGINDQP
EEEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCH
EHARQLARLMRQFDNAVQSKDAGKVNLIPFNPFPGTRYERSGETEIRAFQKILLDAQVLT
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
MVRRTRGDDIDAACGQLKGQVMDRTRRQAEFRRTLEGQADRDAAA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SRIVNEVVIPSVLQDGSVRTPEPRKQNLLDLDREGLERFFADTLGEARYRAHQVMKWIH
CHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HRYVTDFDQMTDLGKALRAKLHQHAEVLVPNVVFDKPSADGTHKWLLAMGTDGKNAIETV
HHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEEEEEECCCCCCCEEEE
YIPDKGRGTLCVSSQVGCGLNCSFCSTATQGFNRNLTTAEIIGQVWVAARHLGNVPHQQR
EECCCCCCEEEECCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHH
RLTNVVMMGMGEPLMNFDNVVRAMSVMRDDLGYGLASKRVTLSTSGLVPMIDRLSTESDV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHCCCCCCC
SLAVSLHAANDALRESLVPLNKKYPIAELMESCARYLRGSKKRDSVTFEYTLMKGINDQP
EEEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCH
EHARQLARLMRQFDNAVQSKDAGKVNLIPFNPFPGTRYERSGETEIRAFQKILLDAQVLT
HHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
MVRRTRGDDIDAACGQLKGQVMDRTRRQAEFRRTLEGQADRDAAA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]