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The map label for this gene is dgoA

Identifier: 21242511

GI number: 21242511

Start: 2021322

End: 2022470

Strand: Reverse

Name: dgoA

Synonym: XAC1765

Alternate gene names: 21242511

Gene position: 2022470-2021322 (Counterclockwise)

Preceding gene: 21242512

Following gene: 21242510

Centisome position: 39.08

GC content: 64.4

Gene sequence:

>1149_bases
ATGAAGATCACCCGCCTCACCACCTACCACGCCGCACCGCGCTGGCTGTTCCTCAAGGTCGAGACCGACGAGGGCATCAC
CGGCTGGGGAGAGCCGGTCATCGAAGGCCGCGCGCGTTCGGTGGAGGCCGCCGTGCACGAACTGGCCGGCTATGTCGTGG
GCAAGGATCCGGCGCGCATCAACGACCTGTGGCAAACCCTGTACCGCGCCGGCTTTTATCGCGGCGGCGCCATCCTGATG
AGCGCCATCGCCGGTATCGACCAGGCGCTGTGGGACATCAAAGGCAAGGCGCTGGGCGTGCCGGTGTACGAATTGCTGGG
CGGGCTGGTGCGCGACCGCATGAAGACCTATCGCTGGGTCGGCGGCGACCGCCCGGGCGCGATCATCCAGCAGATCACCG
ACTACCGCGCGCTGGGTTTCGACACCTTCAAGTTCAACGGTACCGAAGAAATGAAGCTGATCGACAGCGCGCGCGCAGTC
GATGCGGCGGTGGTCAAGGTGGCTGAAATTCGCGGAGCCTTCGGCAACACCATCGACTTCGGCATCGATTTCCATGGCCG
CGTCGGCGCGCCGATGGCCAAGGCGTTGTTGCGCGAGCTCGAACCGTTCAAGCCCCTGTTCGTCGAGGAGCCGGTGCTGG
CCGAACAGGCCGAGTATTACCCGCGTCTGGCCGCAAGCACTTCGATTCCGCTCGCCGCCGGCGAACGCATGTTCTCGCGC
TTCGAATTCAAGAACGTGCTGTGCGCCGGCGGCATCGGCATGGTGCAACCGGACCTGTCGCATGCCGGCGGCATCACCGA
ATGCGTCAAGATCGCCGCGATGGCCGAAGCCTACGACGTCGGCTTCGCCCCGCACTGCCCGCTCGGCCCGATCGCACTCG
CCGCGTGCCTGCACGTGGACTTCGTTTCGCACAACGCCGTGCTGCAGGAACAGAGCATCGGCATCCACTACAACGAAGGC
GCGGATCTGCTCGATTACGTCGTCAACAAAGACGATTTTCACTGCGTGGATGGCAGCATTGCCGCACTGCCCAAACCCGG
GCTCGGTGTGGAGATCGACGAAGAGATGCTCAAGCGCGCAAACGAAAACCCGCCCGACTGGCGCAACCCGGTATGGCGCC
ACAGCGATGGCAGCATCGCCGAATGGTAA

Upstream 100 bases:

>100_bases
GCGCCGGCATCGGTGGCGAACTGTACAAACCCGGCCAATCGCTCGAAACAACCCAGACGCATGCACGCGCCTTTGTGCAG
GCCTACCAGGAACTGCAAGG

Downstream 100 bases:

>100_bases
GCACGCCCGAACACACCGCCGTGCTGGCGGTCGACAGCCGCTGCACACATGGCGAAGGCGTGCTGTGGTGCGAGCAACGC
CAGGCGCTGTACTGGGTCGA

Product: galactonate dehydratase

Products: NA

Alternate protein names: GalD

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MKITRLTTYHAAPRWLFLKVETDEGITGWGEPVIEGRARSVEAAVHELAGYVVGKDPARINDLWQTLYRAGFYRGGAILM
SAIAGIDQALWDIKGKALGVPVYELLGGLVRDRMKTYRWVGGDRPGAIIQQITDYRALGFDTFKFNGTEEMKLIDSARAV
DAAVVKVAEIRGAFGNTIDFGIDFHGRVGAPMAKALLRELEPFKPLFVEEPVLAEQAEYYPRLAASTSIPLAAGERMFSR
FEFKNVLCAGGIGMVQPDLSHAGGITECVKIAAMAEAYDVGFAPHCPLGPIALAACLHVDFVSHNAVLQEQSIGIHYNEG
ADLLDYVVNKDDFHCVDGSIAALPKPGLGVEIDEEMLKRANENPPDWRNPVWRHSDGSIAEW

Sequences:

>Translated_382_residues
MKITRLTTYHAAPRWLFLKVETDEGITGWGEPVIEGRARSVEAAVHELAGYVVGKDPARINDLWQTLYRAGFYRGGAILM
SAIAGIDQALWDIKGKALGVPVYELLGGLVRDRMKTYRWVGGDRPGAIIQQITDYRALGFDTFKFNGTEEMKLIDSARAV
DAAVVKVAEIRGAFGNTIDFGIDFHGRVGAPMAKALLRELEPFKPLFVEEPVLAEQAEYYPRLAASTSIPLAAGERMFSR
FEFKNVLCAGGIGMVQPDLSHAGGITECVKIAAMAEAYDVGFAPHCPLGPIALAACLHVDFVSHNAVLQEQSIGIHYNEG
ADLLDYVVNKDDFHCVDGSIAALPKPGLGVEIDEEMLKRANENPPDWRNPVWRHSDGSIAEW
>Mature_382_residues
MKITRLTTYHAAPRWLFLKVETDEGITGWGEPVIEGRARSVEAAVHELAGYVVGKDPARINDLWQTLYRAGFYRGGAILM
SAIAGIDQALWDIKGKALGVPVYELLGGLVRDRMKTYRWVGGDRPGAIIQQITDYRALGFDTFKFNGTEEMKLIDSARAV
DAAVVKVAEIRGAFGNTIDFGIDFHGRVGAPMAKALLRELEPFKPLFVEEPVLAEQAEYYPRLAASTSIPLAAGERMFSR
FEFKNVLCAGGIGMVQPDLSHAGGITECVKIAAMAEAYDVGFAPHCPLGPIALAACLHVDFVSHNAVLQEQSIGIHYNEG
ADLLDYVVNKDDFHCVDGSIAALPKPGLGVEIDEEMLKRANENPPDWRNPVWRHSDGSIAEW

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily

Homologues:

Organism=Escherichia coli, GI48994953, Length=382, Percent_Identity=73.5602094240838, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI1787864, Length=413, Percent_Identity=29.5399515738499, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI226510960, Length=275, Percent_Identity=30.1818181818182, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DGOD_XANAC (Q8PLN0)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642093.1
- HSSP:   Q8ZNH1
- ProteinModelPortal:   Q8PLN0
- GeneID:   1155836
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1765
- NMPDR:   fig|190486.1.peg.1737
- HOGENOM:   HBG705576
- OMA:   KADFALE
- ProtClustDB:   PRK14017
- BioCyc:   XAXO190486:XAC1765-MONOMER
- HAMAP:   MF_01289
- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354
- PANTHER:   PTHR13794
- SMART:   SM00922

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N

EC number: =4.2.1.6

Molecular weight: Translated: 41854; Mature: 41854

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00908 MR_MLE_1; PS00909 MR_MLE_2

Important sites: ACT_SITE 185-185 ACT_SITE 285-285

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITRLTTYHAAPRWLFLKVETDEGITGWGEPVIEGRARSVEAAVHELAGYVVGKDPARI
CCEEEEEEECCCCEEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCHHH
NDLWQTLYRAGFYRGGAILMSAIAGIDQALWDIKGKALGVPVYELLGGLVRDRMKTYRWV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHC
GGDRPGAIIQQITDYRALGFDTFKFNGTEEMKLIDSARAVDAAVVKVAEIRGAFGNTIDF
CCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
GIDFHGRVGAPMAKALLRELEPFKPLFVEEPVLAEQAEYYPRLAASTSIPLAAGERMFSR
CEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
FEFKNVLCAGGIGMVQPDLSHAGGITECVKIAAMAEAYDVGFAPHCPLGPIALAACLHVD
HHHHHHHEECCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
FVSHNAVLQEQSIGIHYNEGADLLDYVVNKDDFHCVDGSIAALPKPGLGVEIDEEMLKRA
HHHCCCEEECCCCCEEECCCCHHHHHHHCCCCCEEECCCEEECCCCCCCCEECHHHHHHC
NENPPDWRNPVWRHSDGSIAEW
CCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKITRLTTYHAAPRWLFLKVETDEGITGWGEPVIEGRARSVEAAVHELAGYVVGKDPARI
CCEEEEEEECCCCEEEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCHHH
NDLWQTLYRAGFYRGGAILMSAIAGIDQALWDIKGKALGVPVYELLGGLVRDRMKTYRWV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHC
GGDRPGAIIQQITDYRALGFDTFKFNGTEEMKLIDSARAVDAAVVKVAEIRGAFGNTIDF
CCCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
GIDFHGRVGAPMAKALLRELEPFKPLFVEEPVLAEQAEYYPRLAASTSIPLAAGERMFSR
CEECCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
FEFKNVLCAGGIGMVQPDLSHAGGITECVKIAAMAEAYDVGFAPHCPLGPIALAACLHVD
HHHHHHHEECCCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
FVSHNAVLQEQSIGIHYNEGADLLDYVVNKDDFHCVDGSIAALPKPGLGVEIDEEMLKRA
HHHCCCEEECCCCCEEECCCCHHHHHHHCCCCCEEECCCEEECCCCCCCCEECHHHHHHC
NENPPDWRNPVWRHSDGSIAEW
CCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217