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The map label for this gene is lexA

Identifier: 21242488

GI number: 21242488

Start: 1999076

End: 1999717

Strand: Direct

Name: lexA

Synonym: XAC1739

Alternate gene names: 21242488

Gene position: 1999076-1999717 (Clockwise)

Preceding gene: 21242487

Following gene: 21242489

Centisome position: 38.63

GC content: 64.64

Gene sequence:

>642_bases
ATGGACCTGACCGATACCCAGCAAGCAATCCTGGCCCTGATCGCCGAGCGCATCGACGCCGATGGTGTTCCGCCTTCGCA
GACCGAAATCGCGCGCGCGTTCGGCTTCAAGGGCATACGCGCGGCGCAATATCACCTGGAAGCGCTGGAGCATGCGGGTG
CGATCCGGCGCGTCCCAGGCCAGGCGCGCGGGATACGGCTGGCCGGGCAGGGTGCGCAGACACGCACGGCTCCTGTGAGC
GAGGTGGCCCGCGACGATGTGCTGCGTCTGCCGGTGCTGGGCCGCGTGGCCGCGGGTCTGCCGATTGGCGCCGATATCGG
CTCGGACGACTTCGTCGTGCTGGATCGTGTGTTTTTCTCGCCGTCGCCGGATTATCTGCTCAAGGTGCAAGGCGATTCGA
TGCGCGACGAAGGCATCTTCAACGGCGATCTGATCGGCGTGCACCGTACCCGCGACGCGCGCTCGGGGCAGATCGTGGTG
GCGCGGATCGATGAGGAAATCACGGTAAAGCTGCTGAAGATCGGCAAGGACCGCATCCGGCTGTTGCCGCGCAATCCAGA
CTACGCGCCCATTGAGGTGCTACCGGATCAGGATTTCGCGATTGAAGGCCTGTATTGCGGGCTGTTGAGGCCTAACCGCT
GA

Upstream 100 bases:

>100_bases
CGAAGCCGGCGCTAGCGCGCGACGCGTCGTCCTGATGGCGTGCGGGCAAGACGCGGCGAAATACTTGCGCAGGCGATCGT
TAGTAGTAATACTACTAAAC

Downstream 100 bases:

>100_bases
GCTCTGTGCCGATGGCCGTGCTGGCGTTTCTCCGAGCCCGCACTGCAGGATTTCCCGCTGCCACGGGACTTGCCGCCTTC
TTAATCTGAGTGTGTCGCCT

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MDLTDTQQAILALIAERIDADGVPPSQTEIARAFGFKGIRAAQYHLEALEHAGAIRRVPGQARGIRLAGQGAQTRTAPVS
EVARDDVLRLPVLGRVAAGLPIGADIGSDDFVVLDRVFFSPSPDYLLKVQGDSMRDEGIFNGDLIGVHRTRDARSGQIVV
ARIDEEITVKLLKIGKDRIRLLPRNPDYAPIEVLPDQDFAIEGLYCGLLRPNR

Sequences:

>Translated_213_residues
MDLTDTQQAILALIAERIDADGVPPSQTEIARAFGFKGIRAAQYHLEALEHAGAIRRVPGQARGIRLAGQGAQTRTAPVS
EVARDDVLRLPVLGRVAAGLPIGADIGSDDFVVLDRVFFSPSPDYLLKVQGDSMRDEGIFNGDLIGVHRTRDARSGQIVV
ARIDEEITVKLLKIGKDRIRLLPRNPDYAPIEVLPDQDFAIEGLYCGLLRPNR
>Mature_213_residues
MDLTDTQQAILALIAERIDADGVPPSQTEIARAFGFKGIRAAQYHLEALEHAGAIRRVPGQARGIRLAGQGAQTRTAPVS
EVARDDVLRLPVLGRVAAGLPIGADIGSDDFVVLDRVFFSPSPDYLLKVQGDSMRDEGIFNGDLIGVHRTRDARSGQIVV
ARIDEEITVKLLKIGKDRIRLLPRNPDYAPIEVLPDQDFAIEGLYCGLLRPNR

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. Has been shown to bind to the palindromic sequence 5'-CTG-N(8-12)-C-[TC]-G. In the presence of single-stranded DNA, recA interacts with lexA causin

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=209, Percent_Identity=43.5406698564593, Blast_Score=161, Evalue=3e-41,
Organism=Escherichia coli, GI1787431, Length=93, Percent_Identity=36.5591397849462, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA2_XANAC (P60511)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642070.1
- ProteinModelPortal:   P60511
- SMR:   P60511
- GeneID:   1155810
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1739
- NMPDR:   fig|190486.1.peg.1714
- HOGENOM:   HBG679610
- OMA:   ITNHINA
- ProtClustDB:   PRK00215
- BioCyc:   XAXO190486:XAC1739-MONOMER
- BRENDA:   3.4.21.88
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 23240; Mature: 23240

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: ACT_SITE 133-133 ACT_SITE 170-170

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTDTQQAILALIAERIDADGVPPSQTEIARAFGFKGIRAAQYHLEALEHAGAIRRVPG
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHCCCC
QARGIRLAGQGAQTRTAPVSEVARDDVLRLPVLGRVAAGLPIGADIGSDDFVVLDRVFFS
CCCCEEEECCCCCCCCCCHHHHHHHCEEECCHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PSPDYLLKVQGDSMRDEGIFNGDLIGVHRTRDARSGQIVVARIDEEITVKLLKIGKDRIR
CCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHHCCCCEE
LLPRNPDYAPIEVLPDQDFAIEGLYCGLLRPNR
EECCCCCCCCEEEECCCCCEECEEEEEECCCCC
>Mature Secondary Structure
MDLTDTQQAILALIAERIDADGVPPSQTEIARAFGFKGIRAAQYHLEALEHAGAIRRVPG
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHCCCC
QARGIRLAGQGAQTRTAPVSEVARDDVLRLPVLGRVAAGLPIGADIGSDDFVVLDRVFFS
CCCCEEEECCCCCCCCCCHHHHHHHCEEECCHHHHHHCCCCCCCCCCCCCEEEEEEECCC
PSPDYLLKVQGDSMRDEGIFNGDLIGVHRTRDARSGQIVVARIDEEITVKLLKIGKDRIR
CCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCHHHHHHHHHCCCCEE
LLPRNPDYAPIEVLPDQDFAIEGLYCGLLRPNR
EECCCCCCCCEEEECCCCCEECEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217