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The map label for this gene is miaA

Identifier: 21242483

GI number: 21242483

Start: 1993285

End: 1994268

Strand: Direct

Name: miaA

Synonym: XAC1734

Alternate gene names: 21242483

Gene position: 1993285-1994268 (Clockwise)

Preceding gene: 21242482

Following gene: 21242484

Centisome position: 38.51

GC content: 69.31

Gene sequence:

>984_bases
ATGGCTGTCGACCAACGCCCGCTGGCGATCGCCGTGATGGGCCCCACTGCCAGCGGCAAGACGGCGCTGGCGCTTGAGGC
TGCAGAGCGCTGGAATGGAGAAATCGTCAGCGTCGATTCGGCGCTGGTGTACCGCGGCCTGGAAATCGGCGCAGCCAAGC
CGGATGCGGCGATGCGTGCGGCGGTTCCGCATCATCTGCTCGACCTGCGCGATCCCTGGCAGGTGTATTCGGCAGCGGAG
TTCGCCGCCGATGCACGCCAGGCGATCGCGCAGATCGTGGCGCGCGGCAGGTTGCCGATTCTGGCCGGCGGCACGGGGCT
GTACTTTCGTGCGCTGCTGGAAGGTTTGTCGCAGCTGCCCGAGGCCGACCAGGCCGTGCGTGCATCCATCGCCGCCGAAG
CTCAGCAGATCGGTTGGGCCGGCTTGCACGCACAACTGGCACGCGTGGACCCGGTGGCAGCAGCGCGCATCCATGCGACC
GACCCGCAGCGCATCCAGCGGGCGCTGGAGGTCTACCGCATCAGCGGGCGGCCGATCAGCGCGTGGCAGGCGCTACCGCC
AGGGCCGCGCCTGCCGGTGCGCGTGTTGAAGGTGGTATTGGCGCCACGCGAGCGCGCGGTGCTGCATGCACGTATCGCAC
ACCGGCTGGATGCGATGCTGGCGCAGGATTTTCTGACCGAAGTGCGGCGCCTGCGGGCGCTGCCGCAGCTCCAGGCGGTG
GCAGCCCCGCTGGATCTGCCGGCCGTGCGCGCGGTCGGCTACCGCCAGGCCTGGCAGTATCTGGACGGGGCTGGCAGCCT
GGCCGAGTTCCGCGACAAGGCGATCCAGGCGACCCGGCAACTGGCCAAACGCCAGCTCACCTGGCTGCGCGGCGAGCTCG
ACGCGCGCTGGTTCGACCCGGAGCGTGACCGCCATCAATTGGAGGACGCAATTGTCGGCTTCCTCGCTGATCGTCCCGCT
GTACGGCAGGCTTCAGGCGTGTAA

Upstream 100 bases:

>100_bases
CCGTGGATGCGTTGAAGGTGTGGAAGGCGATGGCGGCCATTCCCGTCCCGCGTGTCAGCGCGCCCGCCACGCCCGCGATG
CGTTGGCCGGACGAGGACTG

Downstream 100 bases:

>100_bases
CATCCCGTTTCCGGGACCGGCTGGGGATAGCAGGTGTCGACCGGAACAATAAGAACAATAATGAAGAGGATTTTTCGATG
GCTAAGGGGCAATCTTTACA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MAVDQRPLAIAVMGPTASGKTALALEAAERWNGEIVSVDSALVYRGLEIGAAKPDAAMRAAVPHHLLDLRDPWQVYSAAE
FAADARQAIAQIVARGRLPILAGGTGLYFRALLEGLSQLPEADQAVRASIAAEAQQIGWAGLHAQLARVDPVAAARIHAT
DPQRIQRALEVYRISGRPISAWQALPPGPRLPVRVLKVVLAPRERAVLHARIAHRLDAMLAQDFLTEVRRLRALPQLQAV
AAPLDLPAVRAVGYRQAWQYLDGAGSLAEFRDKAIQATRQLAKRQLTWLRGELDARWFDPERDRHQLEDAIVGFLADRPA
VRQASGV

Sequences:

>Translated_327_residues
MAVDQRPLAIAVMGPTASGKTALALEAAERWNGEIVSVDSALVYRGLEIGAAKPDAAMRAAVPHHLLDLRDPWQVYSAAE
FAADARQAIAQIVARGRLPILAGGTGLYFRALLEGLSQLPEADQAVRASIAAEAQQIGWAGLHAQLARVDPVAAARIHAT
DPQRIQRALEVYRISGRPISAWQALPPGPRLPVRVLKVVLAPRERAVLHARIAHRLDAMLAQDFLTEVRRLRALPQLQAV
AAPLDLPAVRAVGYRQAWQYLDGAGSLAEFRDKAIQATRQLAKRQLTWLRGELDARWFDPERDRHQLEDAIVGFLADRPA
VRQASGV
>Mature_326_residues
AVDQRPLAIAVMGPTASGKTALALEAAERWNGEIVSVDSALVYRGLEIGAAKPDAAMRAAVPHHLLDLRDPWQVYSAAEF
AADARQAIAQIVARGRLPILAGGTGLYFRALLEGLSQLPEADQAVRASIAAEAQQIGWAGLHAQLARVDPVAAARIHATD
PQRIQRALEVYRISGRPISAWQALPPGPRLPVRVLKVVLAPRERAVLHARIAHRLDAMLAQDFLTEVRRLRALPQLQAVA
APLDLPAVRAVGYRQAWQYLDGAGSLAEFRDKAIQATRQLAKRQLTWLRGELDARWFDPERDRHQLEDAIVGFLADRPAV
RQASGV

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=317, Percent_Identity=29.6529968454259, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1790613, Length=296, Percent_Identity=53.7162162162162, Blast_Score=290, Evalue=9e-80,
Organism=Caenorhabditis elegans, GI25144712, Length=232, Percent_Identity=26.7241379310345, Blast_Score=91, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6324848, Length=292, Percent_Identity=25.3424657534247, Blast_Score=119, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24649830, Length=265, Percent_Identity=33.2075471698113, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_XANAC (Q8PLQ5)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_642065.1
- ProteinModelPortal:   Q8PLQ5
- SMR:   Q8PLQ5
- GeneID:   1155805
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1734
- NMPDR:   fig|190486.1.peg.1709
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   XAXO190486:XAC1734-MONOMER
- BRENDA:   2.5.1.8
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35690; Mature: 35558

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVDQRPLAIAVMGPTASGKTALALEAAERWNGEIVSVDSALVYRGLEIGAAKPDAAMRA
CCCCCCCEEEEEECCCCCCCEEEEEEHHHHCCCCEEEECHHHHHHCCCCCCCCCCHHHHH
AVPHHLLDLRDPWQVYSAAEFAADARQAIAQIVARGRLPILAGGTGLYFRALLEGLSQLP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHCC
EADQAVRASIAAEAQQIGWAGLHAQLARVDPVAAARIHATDPQRIQRALEVYRISGRPIS
CHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHEEECCCHHHHHHHHHHHHCCCCCCC
AWQALPPGPRLPVRVLKVVLAPRERAVLHARIAHRLDAMLAQDFLTEVRRLRALPQLQAV
CCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
AAPLDLPAVRAVGYRQAWQYLDGAGSLAEFRDKAIQATRQLAKRQLTWLRGELDARWFDP
HCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ERDRHQLEDAIVGFLADRPAVRQASGV
CHHHHHHHHHHHHHHHCCCHHHHCCCC
>Mature Secondary Structure 
AVDQRPLAIAVMGPTASGKTALALEAAERWNGEIVSVDSALVYRGLEIGAAKPDAAMRA
CCCCCCEEEEEECCCCCCCEEEEEEHHHHCCCCEEEECHHHHHHCCCCCCCCCCHHHHH
AVPHHLLDLRDPWQVYSAAEFAADARQAIAQIVARGRLPILAGGTGLYFRALLEGLSQLP
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHHHCC
EADQAVRASIAAEAQQIGWAGLHAQLARVDPVAAARIHATDPQRIQRALEVYRISGRPIS
CHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCHHHHEEECCCHHHHHHHHHHHHCCCCCCC
AWQALPPGPRLPVRVLKVVLAPRERAVLHARIAHRLDAMLAQDFLTEVRRLRALPQLQAV
CCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
AAPLDLPAVRAVGYRQAWQYLDGAGSLAEFRDKAIQATRQLAKRQLTWLRGELDARWFDP
HCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
ERDRHQLEDAIVGFLADRPAVRQASGV
CHHHHHHHHHHHHHHHCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217