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The map label for this gene is pitA [H]

Identifier: 21242460

GI number: 21242460

Start: 1970822

End: 1971949

Strand: Direct

Name: pitA [H]

Synonym: XAC1711

Alternate gene names: 21242460

Gene position: 1970822-1971949 (Clockwise)

Preceding gene: 21242459

Following gene: 21242461

Centisome position: 38.08

GC content: 65.07

Gene sequence:

>1128_bases
GTGCTTACCTTAGTTCTGGTGGTGATCCTGGCCGCGTTGGTGTTCGAGTTCATCAACGGCTTCCACGACACCGCCAACTC
CATCGCCACCGTGGTTGCCACCAAGGTGCTCTCGCCCGGTTGGGCGGTGATGCTGGCAGCCGGCATGAACCTGATCGGCG
CGCTGACCGGCACCGCCGTGGCGCTGACGATTGCTTCTGGCCTGCTCAACACCAACGTGGTCGAGGTCACCCCGCAGGTG
ATTTTGTGCGCGCTGCTGGGCGGCATCATCTGGAACCTGATCACCTGGTGGAAGGGCCTGCCGTCGTCGTCCTCGCATGC
CTTGATCGGCGGCCTGTGCGGCGCCGGTCTGGCCGCGGCGCACAACGACTGGGACGCGCTGATCTGGTCGGAAAACATCG
GCAACTGGGCCAAGAACAAGGGCCTGTTGTGGAAGGTGTTCGTCCCGATGATCACCTCGCCGATCGCCGGTTTCGTGCTC
GGTATCGTGGTGATGGTGCTGCTGTGGGCGATCATTGCCGGCATGTCCAAGATAGGCGGCCCGATCGGGCGGCTGGCGCG
GCCGCGCTGGGTCAATGCGTTCTTCGGCAAGGCGCAGATCGCCTCGGCCGCATACATGGGCTACGCGCACGGCCACAACG
ACGCGCAGAAGACCATGGGCATCATCGCCATGACCCTGATCGGCGCGCAGTCGGCCGGCGCGCTGGACGACCTGCCGAGC
TGGTTGTCGTTCCTGCATCCGGGCACCGGCCTTGCGGCCGGCGCCGGTATCCCGATGTGGATCGTGCTCACCTGCGCGGT
GGTGATGGCTGCAGGTACCGCGTCGGGCGGTTGGAAGATCATCAAGACGCTCGGCCACAAGATGGTCAAGCTGCATCCCA
TCCACGGCTTTGCCGCAGAAACCAGCTCGGCGACGGTGCTCACCGTGGCGGCGCATTTCGGCATGCCGGTGTCCACCACG
CACAGCATTTCCACCGCCATCATGGGGGTGGGTTTCGCCAAGAATCCGCGTTCGCTGCGGCTGGGCGTGATCGAGCGCAT
CGTCTGGGCCTGGATCCTGACCATCCCGGCTGCTGGCGGCGTGGCGTACCTGATCCTGACGCTGTTCGAGCTGTTGGGCT
GGACCTGA

Upstream 100 bases:

>100_bases
CAAGGAGTTCTTCGAGATTCTGGAAAAGGCCATCGACCGCTGCCGCGAGGCCGGTGTGGTGGTCTACCAGATCGTGCTGA
AGAACAGCTGAGGTCGCCGC

Downstream 100 bases:

>100_bases
GCGGTGCCGCGGTGCGGTCACGCACCGTTGGCAGACTGCGATGCCCTTGCCGTATGGACCTGTTTCCGGCAGGTTGCCCG
CATGCCATGAGTGAGACCCT

Product: transport protein

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MLTLVLVVILAALVFEFINGFHDTANSIATVVATKVLSPGWAVMLAAGMNLIGALTGTAVALTIASGLLNTNVVEVTPQV
ILCALLGGIIWNLITWWKGLPSSSSHALIGGLCGAGLAAAHNDWDALIWSENIGNWAKNKGLLWKVFVPMITSPIAGFVL
GIVVMVLLWAIIAGMSKIGGPIGRLARPRWVNAFFGKAQIASAAYMGYAHGHNDAQKTMGIIAMTLIGAQSAGALDDLPS
WLSFLHPGTGLAAGAGIPMWIVLTCAVVMAAGTASGGWKIIKTLGHKMVKLHPIHGFAAETSSATVLTVAAHFGMPVSTT
HSISTAIMGVGFAKNPRSLRLGVIERIVWAWILTIPAAGGVAYLILTLFELLGWT

Sequences:

>Translated_375_residues
MLTLVLVVILAALVFEFINGFHDTANSIATVVATKVLSPGWAVMLAAGMNLIGALTGTAVALTIASGLLNTNVVEVTPQV
ILCALLGGIIWNLITWWKGLPSSSSHALIGGLCGAGLAAAHNDWDALIWSENIGNWAKNKGLLWKVFVPMITSPIAGFVL
GIVVMVLLWAIIAGMSKIGGPIGRLARPRWVNAFFGKAQIASAAYMGYAHGHNDAQKTMGIIAMTLIGAQSAGALDDLPS
WLSFLHPGTGLAAGAGIPMWIVLTCAVVMAAGTASGGWKIIKTLGHKMVKLHPIHGFAAETSSATVLTVAAHFGMPVSTT
HSISTAIMGVGFAKNPRSLRLGVIERIVWAWILTIPAAGGVAYLILTLFELLGWT
>Mature_375_residues
MLTLVLVVILAALVFEFINGFHDTANSIATVVATKVLSPGWAVMLAAGMNLIGALTGTAVALTIASGLLNTNVVEVTPQV
ILCALLGGIIWNLITWWKGLPSSSSHALIGGLCGAGLAAAHNDWDALIWSENIGNWAKNKGLLWKVFVPMITSPIAGFVL
GIVVMVLLWAIIAGMSKIGGPIGRLARPRWVNAFFGKAQIASAAYMGYAHGHNDAQKTMGIIAMTLIGAQSAGALDDLPS
WLSFLHPGTGLAAGAGIPMWIVLTCAVVMAAGTASGGWKIIKTLGHKMVKLHPIHGFAAETSSATVLTVAAHFGMPVSTT
HSISTAIMGVGFAKNPRSLRLGVIERIVWAWILTIPAAGGVAYLILTLFELLGWT

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=181, Percent_Identity=24.8618784530387, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI5803173, Length=170, Percent_Identity=27.6470588235294, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1789360, Length=235, Percent_Identity=35.7446808510638, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1789907, Length=234, Percent_Identity=34.1880341880342, Blast_Score=104, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI25146401, Length=179, Percent_Identity=23.463687150838, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17539280, Length=194, Percent_Identity=26.8041237113402, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71981576, Length=171, Percent_Identity=23.9766081871345, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17557328, Length=172, Percent_Identity=26.1627906976744, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17536725, Length=176, Percent_Identity=26.7045454545455, Blast_Score=66, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6319773, Length=176, Percent_Identity=27.2727272727273, Blast_Score=66, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 39136; Mature: 39136

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLVLVVILAALVFEFINGFHDTANSIATVVATKVLSPGWAVMLAAGMNLIGALTGTAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ALTIASGLLNTNVVEVTPQVILCALLGGIIWNLITWWKGLPSSSSHALIGGLCGAGLAAA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHC
HNDWDALIWSENIGNWAKNKGLLWKVFVPMITSPIAGFVLGIVVMVLLWAIIAGMSKIGG
CCCCCCEEEECCCCCHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PIGRLARPRWVNAFFGKAQIASAAYMGYAHGHNDAQKTMGIIAMTLIGAQSAGALDDLPS
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
WLSFLHPGTGLAAGAGIPMWIVLTCAVVMAAGTASGGWKIIKTLGHKMVKLHPIHGFAAE
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEEECCCCCCCC
TSSATVLTVAAHFGMPVSTTHSISTAIMGVGFAKNPRSLRLGVIERIVWAWILTIPAAGG
CCCCEEEEEHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCH
VAYLILTLFELLGWT
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MLTLVLVVILAALVFEFINGFHDTANSIATVVATKVLSPGWAVMLAAGMNLIGALTGTAV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
ALTIASGLLNTNVVEVTPQVILCALLGGIIWNLITWWKGLPSSSSHALIGGLCGAGLAAA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHC
HNDWDALIWSENIGNWAKNKGLLWKVFVPMITSPIAGFVLGIVVMVLLWAIIAGMSKIGG
CCCCCCEEEECCCCCHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PIGRLARPRWVNAFFGKAQIASAAYMGYAHGHNDAQKTMGIIAMTLIGAQSAGALDDLPS
CHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHH
WLSFLHPGTGLAAGAGIPMWIVLTCAVVMAAGTASGGWKIIKTLGHKMVKLHPIHGFAAE
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEEECCCCCCCC
TSSATVLTVAAHFGMPVSTTHSISTAIMGVGFAKNPRSLRLGVIERIVWAWILTIPAAGG
CCCCEEEEEHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCH
VAYLILTLFELLGWT
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]