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The map label for this gene is rlmB

Identifier: 21242319

GI number: 21242319

Start: 1812938

End: 1813687

Strand: Direct

Name: rlmB

Synonym: XAC1569

Alternate gene names: 21242319

Gene position: 1812938-1813687 (Clockwise)

Preceding gene: 21242318

Following gene: 77748595

Centisome position: 35.03

GC content: 68.4

Gene sequence:

>750_bases
ATGAGCAAGCAGAACCAGTGGATCGTCGGCGTCAACGCTGTCGCCTCGTCCGTCGAGAACGACGCCGACAACGTGCGCGA
GGTCTTGATCGAAGCCGGCAGCAAGAATCCGCGCCTGACCGAGATCGAGGAACAGGCGCGCCGCAAGGGTATCGACGTGC
GCCGGGTCAACACCCAGGCGCTGGACGGTGTGGGCGGGCAGGTGCGCCATCAGGGCGTGGCCGCGCGCTATGCCGCCGCG
CGGCTGTGGGCAGAGAACGAGCTGGAAGGGCTGGTCGAGGCCGCCGCAGGGCGCGCGCTGGTGCTGATCCTCGATGGCGT
GCAGGACCCGCACAACCTTGGCGCTTGCCTGCGCAGTGCGGCAGCGGCCGGGGTCACCGCGGTGGTGATTCCCAAGGACA
AGTCGGCCACGGTGAACGCCACCGTGCGCAAGACCTCCGCCGGTGCCGCCGACCGTATTCCGGTGGTGGCGGTGACCAAC
CTGGCGCGCTGCCTGCGCGACCTGCAGAAGCAGGGGGTGTGGCTGTATGGCTTGGCCGGCGAAGCAGAGGCTTCGTTGTA
CAGCGTGGATCTGCGCGGCAATGTCGGGCTGGTTCTGGGTGGCGAGGCCGATGGCCTGCGCCGTCTCACCCGCGAGCACT
GCGACGGCCTGGTCAAGATCCCGATGCCCGGCGAAATCGAAAGCCTCAATGTGTCGGTCGCGACCGGTGTGACCTTGTTC
GAGGCCGTGCGCCAGCGCCTGGGCGCCTAA

Upstream 100 bases:

>100_bases
CAACGCTTCGCTGGCATCGCCCGGGCTCCCATGCACCGGCCGTGACGTGACAGGCCGCAGGCCCTACCATTCCCTCTTTA
CCTCTTCGTATGCCCACGCA

Downstream 100 bases:

>100_bases
CACCTGCGTGCATCAGGCGCGAGCGCAGGCGTGCCGGCCTGGGTATCGCACGCGCGTTACGCGATGCACATCAGCTCCAA
TTCTTGATCGTGGCAGTGCC

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSKQNQWIVGVNAVASSVENDADNVREVLIEAGSKNPRLTEIEEQARRKGIDVRRVNTQALDGVGGQVRHQGVAARYAAA
RLWAENELEGLVEAAAGRALVLILDGVQDPHNLGACLRSAAAAGVTAVVIPKDKSATVNATVRKTSAGAADRIPVVAVTN
LARCLRDLQKQGVWLYGLAGEAEASLYSVDLRGNVGLVLGGEADGLRRLTREHCDGLVKIPMPGEIESLNVSVATGVTLF
EAVRQRLGA

Sequences:

>Translated_249_residues
MSKQNQWIVGVNAVASSVENDADNVREVLIEAGSKNPRLTEIEEQARRKGIDVRRVNTQALDGVGGQVRHQGVAARYAAA
RLWAENELEGLVEAAAGRALVLILDGVQDPHNLGACLRSAAAAGVTAVVIPKDKSATVNATVRKTSAGAADRIPVVAVTN
LARCLRDLQKQGVWLYGLAGEAEASLYSVDLRGNVGLVLGGEADGLRRLTREHCDGLVKIPMPGEIESLNVSVATGVTLF
EAVRQRLGA
>Mature_248_residues
SKQNQWIVGVNAVASSVENDADNVREVLIEAGSKNPRLTEIEEQARRKGIDVRRVNTQALDGVGGQVRHQGVAARYAAAR
LWAENELEGLVEAAAGRALVLILDGVQDPHNLGACLRSAAAAGVTAVVIPKDKSATVNATVRKTSAGAADRIPVVAVTNL
ARCLRDLQKQGVWLYGLAGEAEASLYSVDLRGNVGLVLGGEADGLRRLTREHCDGLVKIPMPGEIESLNVSVATGVTLFE
AVRQRLGA

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily

Homologues:

Organism=Homo sapiens, GI40068479, Length=263, Percent_Identity=30.7984790874525, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1790623, Length=242, Percent_Identity=48.7603305785124, Blast_Score=240, Evalue=5e-65,
Organism=Escherichia coli, GI1788935, Length=158, Percent_Identity=33.5443037974684, Blast_Score=79, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324775, Length=282, Percent_Identity=26.9503546099291, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMB_XANAC (Q8PM64)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641901.1
- ProteinModelPortal:   Q8PM64
- SMR:   Q8PM64
- GeneID:   1155640
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1569
- NMPDR:   fig|190486.1.peg.1545
- HOGENOM:   HBG714057
- OMA:   VEGVHQG
- ProtClustDB:   CLSK446414
- BioCyc:   XAXO190486:XAC1569-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01887
- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123
- SMART:   SM00967
- TIGRFAMs:   TIGR00186

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26299; Mature: 26168

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: BINDING 200-200 BINDING 220-220 BINDING 229-229

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQNQWIVGVNAVASSVENDADNVREVLIEAGSKNPRLTEIEEQARRKGIDVRRVNTQA
CCCCCCEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEECCHHH
LDGVGGQVRHQGVAARYAAARLWAENELEGLVEAAAGRALVLILDGVQDPHNLGACLRSA
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHH
AAAGVTAVVIPKDKSATVNATVRKTSAGAADRIPVVAVTNLARCLRDLQKQGVWLYGLAG
HHCCEEEEEEECCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECC
EAEASLYSVDLRGNVGLVLGGEADGLRRLTREHCDGLVKIPMPGEIESLNVSVATGVTLF
CCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHCCCEEECCCCCCCCEEEEEHHHHHHHH
EAVRQRLGA
HHHHHHHCC
>Mature Secondary Structure 
SKQNQWIVGVNAVASSVENDADNVREVLIEAGSKNPRLTEIEEQARRKGIDVRRVNTQA
CCCCCEEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEECCHHH
LDGVGGQVRHQGVAARYAAARLWAENELEGLVEAAAGRALVLILDGVQDPHNLGACLRSA
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHH
AAAGVTAVVIPKDKSATVNATVRKTSAGAADRIPVVAVTNLARCLRDLQKQGVWLYGLAG
HHCCEEEEEEECCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECC
EAEASLYSVDLRGNVGLVLGGEADGLRRLTREHCDGLVKIPMPGEIESLNVSVATGVTLF
CCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHCCCEEECCCCCCCCEEEEEHHHHHHHH
EAVRQRLGA
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217