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The map label for this gene is fumB [H]

Identifier: 21242212

GI number: 21242212

Start: 1686896

End: 1688413

Strand: Direct

Name: fumB [H]

Synonym: XAC1460

Alternate gene names: 21242212

Gene position: 1686896-1688413 (Clockwise)

Preceding gene: 21242209

Following gene: 21242213

Centisome position: 32.59

GC content: 65.09

Gene sequence:

>1518_bases
GTGACCTCGATCAAGCAGGAAGACCTCATCCAGTCCGTCGCCGATGCGCTGCAGTACATCAGCTACTACCACCCGGTCGA
TTACATCAAGAACCTGTCCGCCGCCTACGAGCGCGAAGAATCGCCGGCCGCCAAGGACGCCATTGCGCAGATCCTGATCA
ATTCGCGTATGTGCGCCGAAGGCCACCGCCCGATCTGCCAGGACACCGGCATCGTCACCGTGTTCCTGGAAATCGGCATG
AACGTGCGATGGGACGACGCCACGCTGGGCGTGGAAGACATGGTCAACGAGGGCGTGCGCCGCGCCTATAACCACCCCGA
CAACAAGCTGCGCGCCAGCGTGCTGGCCGATCCGGCCGGCAAGCGCGCCAACACCCGCGACAACACGCCGGCGGTGGTCA
ACACCAAGATCGTGCCCGGCGACCATGTCGAGGTGATCGTCGCGGCCAAGGGCGGCGGCTCGGAGGCCAAGAGCAAGTTC
GCCATGCTCAATCCGTCCGATTCCATCGTCGACTGGGTGCTCAAGACCGTGCCGACCATGGGCGCCGGCTGGTGCCCGCC
GGGCATGCTCGGCATCGGCATTGGCGGCACCGCAGAGAAGGCGATGCTGCTGGCCAAGGAAGCCTTGATGGAGCCGATCG
ACATCGTCGACCTGCAGGCGCGCGGCCCCTCCAACCGTGCCGAAGAGCTGCGTCTTGAGCTGTACGAAAAGGTCAACGCG
CTGGGCATCGGCGCGCAGGGCCTGGGCGGCCTGACCACGGTGCTGGACATCAAGGTCAAGGATTATCCGACCCATGCCGC
CAACCTGCCGGTGGCGTTGATCCCCAACTGCGCCGCCACCCGCCATGCGCACTTCACGCTGGACGGCAGCGGCCCGGTGA
TGCTGGATCCGCCGTCGCTGGAAGATTGGCCCAAGCTCACCTACAACCCGACCAATGCGCGCCGGGTCAATCTGGACACC
ATCAGCAAGGAAGAAGTGGCCAGCTTCAAACCGGGCGAGACGATCCTGCTCAATGGCAAGCTGCTGACCGGCCGCGATGC
CGCGCACAAGCGCATGATCGACATGCTCAACCGCGGCGAAACCCTGCCGGTGGACTTCACCAACCGCTTCATCTACTACG
TCGGCCCGGTCGATCCGGTGCGCGACGAAGTGGTCGGCCCGGCTGGCCCGACCACCGCCACGCGCATGGACAAGTTCACC
CGGCAGATGCTGGAACAGACCGGCCTGCTCGGCATGGTCGGCAAGTCCGAGCGCGGCGATGCGGCGATCGCCGCGATCCG
CGACAACAAGGCCGTCTACCTGATGGCGGTTGGCGGCTCGGCCTATCTGGTGTCCAAGGCGATCAAGGCCGCGCGCGTGC
TGGCGTTCGAGGATCTCGGCATGGAGGCGATCTACGAATTCGAGGTCAAGGACATGCCGGTGACGGTCGCGGTGGATTCC
AGCGGCGAATCGGTGCACAAGACCGGCCCGCGCCAATGGCAGTCGCGTATCGGCAAGATTCCGGTGGTGGTGGAGTAA

Upstream 100 bases:

>100_bases
CTCCGGCAACGCTCAATCGCATCGATTGACATTGCGCCCGGACGCGCCTGCGGTTTGCTCTAAAATGGCCGGATTGCCCC
CGCCAGCCGTAGAGCCAGCC

Downstream 100 bases:

>100_bases
CTGCGACGATCCAACCGGCTGCACCTGCGTTGCGCAGGCGTGGCCGGTGCCGGTGAGTTGCAGGGACGATGCGCGCATGC
GGCACCGCGTTGAGGGCGTT

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MTSIKQEDLIQSVADALQYISYYHPVDYIKNLSAAYEREESPAAKDAIAQILINSRMCAEGHRPICQDTGIVTVFLEIGM
NVRWDDATLGVEDMVNEGVRRAYNHPDNKLRASVLADPAGKRANTRDNTPAVVNTKIVPGDHVEVIVAAKGGGSEAKSKF
AMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIVDLQARGPSNRAEELRLELYEKVNA
LGIGAQGLGGLTTVLDIKVKDYPTHAANLPVALIPNCAATRHAHFTLDGSGPVMLDPPSLEDWPKLTYNPTNARRVNLDT
ISKEEVASFKPGETILLNGKLLTGRDAAHKRMIDMLNRGETLPVDFTNRFIYYVGPVDPVRDEVVGPAGPTTATRMDKFT
RQMLEQTGLLGMVGKSERGDAAIAAIRDNKAVYLMAVGGSAYLVSKAIKAARVLAFEDLGMEAIYEFEVKDMPVTVAVDS
SGESVHKTGPRQWQSRIGKIPVVVE

Sequences:

>Translated_505_residues
MTSIKQEDLIQSVADALQYISYYHPVDYIKNLSAAYEREESPAAKDAIAQILINSRMCAEGHRPICQDTGIVTVFLEIGM
NVRWDDATLGVEDMVNEGVRRAYNHPDNKLRASVLADPAGKRANTRDNTPAVVNTKIVPGDHVEVIVAAKGGGSEAKSKF
AMLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIVDLQARGPSNRAEELRLELYEKVNA
LGIGAQGLGGLTTVLDIKVKDYPTHAANLPVALIPNCAATRHAHFTLDGSGPVMLDPPSLEDWPKLTYNPTNARRVNLDT
ISKEEVASFKPGETILLNGKLLTGRDAAHKRMIDMLNRGETLPVDFTNRFIYYVGPVDPVRDEVVGPAGPTTATRMDKFT
RQMLEQTGLLGMVGKSERGDAAIAAIRDNKAVYLMAVGGSAYLVSKAIKAARVLAFEDLGMEAIYEFEVKDMPVTVAVDS
SGESVHKTGPRQWQSRIGKIPVVVE
>Mature_504_residues
TSIKQEDLIQSVADALQYISYYHPVDYIKNLSAAYEREESPAAKDAIAQILINSRMCAEGHRPICQDTGIVTVFLEIGMN
VRWDDATLGVEDMVNEGVRRAYNHPDNKLRASVLADPAGKRANTRDNTPAVVNTKIVPGDHVEVIVAAKGGGSEAKSKFA
MLNPSDSIVDWVLKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIVDLQARGPSNRAEELRLELYEKVNAL
GIGAQGLGGLTTVLDIKVKDYPTHAANLPVALIPNCAATRHAHFTLDGSGPVMLDPPSLEDWPKLTYNPTNARRVNLDTI
SKEEVASFKPGETILLNGKLLTGRDAAHKRMIDMLNRGETLPVDFTNRFIYYVGPVDPVRDEVVGPAGPTTATRMDKFTR
QMLEQTGLLGMVGKSERGDAAIAAIRDNKAVYLMAVGGSAYLVSKAIKAARVLAFEDLGMEAIYEFEVKDMPVTVAVDSS
GESVHKTGPRQWQSRIGKIPVVVE

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=470, Percent_Identity=31.9148936170213, Blast_Score=188, Evalue=6e-49,
Organism=Escherichia coli, GI1790564, Length=467, Percent_Identity=31.0492505353319, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI1789442, Length=290, Percent_Identity=28.6206896551724, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1789443, Length=172, Percent_Identity=30.8139534883721, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54670; Mature: 54539

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSIKQEDLIQSVADALQYISYYHPVDYIKNLSAAYEREESPAAKDAIAQILINSRMCAE
CCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHCC
GHRPICQDTGIVTVFLEIGMNVRWDDATLGVEDMVNEGVRRAYNHPDNKLRASVLADPAG
CCCCCCCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
KRANTRDNTPAVVNTKIVPGDHVEVIVAAKGGGSEAKSKFAMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCHHHCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIVDLQARGPSNRAEELRLELYEKVNA
CCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
LGIGAQGLGGLTTVLDIKVKDYPTHAANLPVALIPNCAATRHAHFTLDGSGPVMLDPPSL
EECCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCCCCEEEEECCCCCEEECCCCC
EDWPKLTYNPTNARRVNLDTISKEEVASFKPGETILLNGKLLTGRDAAHKRMIDMLNRGE
CCCCCCEECCCCCEEEECCCCCHHHHHCCCCCCEEEEECEEEECCHHHHHHHHHHHHCCC
TLPVDFTNRFIYYVGPVDPVRDEVVGPAGPTTATRMDKFTRQMLEQTGLLGMVGKSERGD
CCEEEECCCEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCC
AAIAAIRDNKAVYLMAVGGSAYLVSKAIKAARVLAFEDLGMEAIYEFEVKDMPVTVAVDS
EEEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEECC
SGESVHKTGPRQWQSRIGKIPVVVE
CCCCHHCCCHHHHHHHHCCCCEEEC
>Mature Secondary Structure 
TSIKQEDLIQSVADALQYISYYHPVDYIKNLSAAYEREESPAAKDAIAQILINSRMCAE
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHCC
GHRPICQDTGIVTVFLEIGMNVRWDDATLGVEDMVNEGVRRAYNHPDNKLRASVLADPAG
CCCCCCCCCCEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
KRANTRDNTPAVVNTKIVPGDHVEVIVAAKGGGSEAKSKFAMLNPSDSIVDWVLKTVPTM
CCCCCCCCCCCEEEEEECCCCCEEEEEEECCCCCHHHCEEEEECCCHHHHHHHHHHHCCC
GAGWCPPGMLGIGIGGTAEKAMLLAKEALMEPIDIVDLQARGPSNRAEELRLELYEKVNA
CCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHH
LGIGAQGLGGLTTVLDIKVKDYPTHAANLPVALIPNCAATRHAHFTLDGSGPVMLDPPSL
EECCCCCCCCEEEEEEEEEECCCCCCCCCCEEECCCCCCCCCEEEEECCCCCEEECCCCC
EDWPKLTYNPTNARRVNLDTISKEEVASFKPGETILLNGKLLTGRDAAHKRMIDMLNRGE
CCCCCCEECCCCCEEEECCCCCHHHHHCCCCCCEEEEECEEEECCHHHHHHHHHHHHCCC
TLPVDFTNRFIYYVGPVDPVRDEVVGPAGPTTATRMDKFTRQMLEQTGLLGMVGKSERGD
CCEEEECCCEEEEECCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCC
AAIAAIRDNKAVYLMAVGGSAYLVSKAIKAARVLAFEDLGMEAIYEFEVKDMPVTVAVDS
EEEEEEECCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEECC
SGESVHKTGPRQWQSRIGKIPVVVE
CCCCHHCCCHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]