Click here to switch to the map view.

The map label for this gene is lepA [H]

Identifier: 21242075

GI number: 21242075

Start: 1527435

End: 1529240

Strand: Direct

Name: lepA [H]

Synonym: XAC1322

Alternate gene names: 21242075

Gene position: 1527435-1529240 (Clockwise)

Preceding gene: 21242074

Following gene: 21242076

Centisome position: 29.51

GC content: 63.23

Gene sequence:

>1806_bases
ATGTCCTCTGATTCAATGCGGAACATCCGCAATTTCTCCATCATTGCCCACGTCGACCACGGCAAATCCACCCTGGCCGA
CCGCATCATCCAGCTGTGCGGCGGCCTGCAGGCGCGCGAGATGGAGGCCCAGGTGCTCGACTCCAACCCGATCGAGCGCG
AACGCGGCATCACCATCAAGGCGCAGTCGGTGTCCCTGCCGTACACGGCAAAGGACGGGCAGACCTACCACCTGAATTTC
ATCGACACCCCCGGGCACGTCGACTTCTCCTATGAAGTCAGCCGCTCGCTGGCTGCGTGCGAAGGTGCGCTGCTGGTGGT
GGATGCGGCGCAGGGCGTGGAAGCGCAGTCGGTGGCCAACTGCTACACCGCGGTGGAGCAGGGGCTGGAAGTGGTGCCGG
TGCTCAACAAGATCGACCTGCCCACGGCCGATGTCGACCGCGCCAAGGCCGAGATCGAAGCGGTGATCGGCATCGATGCC
GAAGACGCGGTGGCGGTGAGCGCCAAGACCGGTCTGAACATCGATCTGGTGCTGGAAGCGATCGTGCATCGCATCCCGCC
GCCCAAGCCGCGCGACACCGACAAGCTGCAGGCGCTGATCATCGATTCCTGGTTCGACAACTACCTGGGCGTGGTCTCGC
TGGTGCGCGTGATGCAGGGCGAGATCAAGCCCGGCAGCAAGATCCTGGTGATGTCCACCGGGCGCACCCATCTGGTCGAC
AAGGTCGGCGTGTTCACCCCCAAGCGTAAGGAACTGCCGGCGCTCGGCGCCGGCGAGGTGGGCTGGATCAACGCGTCCAT
CAAGGACGTGCACGGCGCACCGGTCGGCGACACCCTGACCCTGGCCGGCGATCCGGCGCCGCATGCCTTGCCCGGCTTCC
AGGAAATGCAGCCGCGCGTGTTCGCCGGTCTGTTCCCGGTCGATGCCGAGGATTACCCGGATCTGCGCGAAGCGTTGGAC
AAGTTGCGCCTGAACGATGCCGCGCTGCGCTTCGAGCCGGAAAGCTCCGAAGCGATGGGCTTCGGCTTCCGCTGCGGCTT
CTTGGGCATGCTGCACATGGAAATCGTGCAGGAGCGCCTGGAGCGCGAGTACAACCTGGACCTGATCAGCACCGCACCGA
CCGTGGTGTATGAAGTGCTCAAGACCGATGGCACGGTCATCAACATGGACAACCCGGCCAAGTTGCCGCAGTTGAACCTG
GTGCAGGAAATCCGCGAGCCCATCATTCGCGCCAACGTCCTCACGCCCGAGGAGTACATCGGCAACATCATCAAGCTGTG
CGAGGAAAAGCGCGGCACCCAGATCGGCATCAACTACCTGGGCAGCCAGGTGCAGATCAGCTACGAGCTGCCGATGGCCG
AGGTGGTGCTGGATTTCTTCGACAAGCTCAAGTCGGTCAGCCGTGGCTACGCCTCGCTGGATTACCACTTCGTGCGTTTC
GACGCCGGCCCGTTCGTGCGCGTGGACGTGCTGATCAACGGCGACAAGGTCGATGCGTTGTCGTTGATCGTGCACCGCGG
CCACGCCGATCGTCGCGGCCGCGAGCTGTGCGAAAAGATGAAAGACCTGATCCCCCGGCAGATGTTCGACGTGGCGATCC
AGGCCGCGATCGGCTCGCAGATCATCTCGCGCTCCACGGTCAAGGCGATGCGCAAGAACGTGCTGGCCAAGTGCTATGGT
GGCGACGTTTCGCGCAAGAAGAAGCTGCTGGAAAAGCAGAAAGAAGGCAAGAAACGCATGAAGCAGGTCGGCCGCGTGGA
GATTCCGCAGGAGGCCTTCCTGGCTGTCCTGCAGATGGACAAGTAG

Upstream 100 bases:

>100_bases
CCGATTCCCCACTCCCGATTCCCAATTCCCGGCCCCAGAGGGGTCGGCCGTGCGATAATGCTGCGTTACCCTGACGACGG
CTGCGCCGGCGCCCACCTCA

Downstream 100 bases:

>100_bases
CAGGGAATGGTGAATCGGGAATCGGGAATCGTAAGAGCCTGCGTGCTCCTGCTGTTCCTATTCCCCATTCGCGATTCTCG
ATTCCCTTTCCGAAGGAACA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MSSDSMRNIRNFSIIAHVDHGKSTLADRIIQLCGGLQAREMEAQVLDSNPIERERGITIKAQSVSLPYTAKDGQTYHLNF
IDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVANCYTAVEQGLEVVPVLNKIDLPTADVDRAKAEIEAVIGIDA
EDAVAVSAKTGLNIDLVLEAIVHRIPPPKPRDTDKLQALIIDSWFDNYLGVVSLVRVMQGEIKPGSKILVMSTGRTHLVD
KVGVFTPKRKELPALGAGEVGWINASIKDVHGAPVGDTLTLAGDPAPHALPGFQEMQPRVFAGLFPVDAEDYPDLREALD
KLRLNDAALRFEPESSEAMGFGFRCGFLGMLHMEIVQERLEREYNLDLISTAPTVVYEVLKTDGTVINMDNPAKLPQLNL
VQEIREPIIRANVLTPEEYIGNIIKLCEEKRGTQIGINYLGSQVQISYELPMAEVVLDFFDKLKSVSRGYASLDYHFVRF
DAGPFVRVDVLINGDKVDALSLIVHRGHADRRGRELCEKMKDLIPRQMFDVAIQAAIGSQIISRSTVKAMRKNVLAKCYG
GDVSRKKKLLEKQKEGKKRMKQVGRVEIPQEAFLAVLQMDK

Sequences:

>Translated_601_residues
MSSDSMRNIRNFSIIAHVDHGKSTLADRIIQLCGGLQAREMEAQVLDSNPIERERGITIKAQSVSLPYTAKDGQTYHLNF
IDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVANCYTAVEQGLEVVPVLNKIDLPTADVDRAKAEIEAVIGIDA
EDAVAVSAKTGLNIDLVLEAIVHRIPPPKPRDTDKLQALIIDSWFDNYLGVVSLVRVMQGEIKPGSKILVMSTGRTHLVD
KVGVFTPKRKELPALGAGEVGWINASIKDVHGAPVGDTLTLAGDPAPHALPGFQEMQPRVFAGLFPVDAEDYPDLREALD
KLRLNDAALRFEPESSEAMGFGFRCGFLGMLHMEIVQERLEREYNLDLISTAPTVVYEVLKTDGTVINMDNPAKLPQLNL
VQEIREPIIRANVLTPEEYIGNIIKLCEEKRGTQIGINYLGSQVQISYELPMAEVVLDFFDKLKSVSRGYASLDYHFVRF
DAGPFVRVDVLINGDKVDALSLIVHRGHADRRGRELCEKMKDLIPRQMFDVAIQAAIGSQIISRSTVKAMRKNVLAKCYG
GDVSRKKKLLEKQKEGKKRMKQVGRVEIPQEAFLAVLQMDK
>Mature_600_residues
SSDSMRNIRNFSIIAHVDHGKSTLADRIIQLCGGLQAREMEAQVLDSNPIERERGITIKAQSVSLPYTAKDGQTYHLNFI
DTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVANCYTAVEQGLEVVPVLNKIDLPTADVDRAKAEIEAVIGIDAE
DAVAVSAKTGLNIDLVLEAIVHRIPPPKPRDTDKLQALIIDSWFDNYLGVVSLVRVMQGEIKPGSKILVMSTGRTHLVDK
VGVFTPKRKELPALGAGEVGWINASIKDVHGAPVGDTLTLAGDPAPHALPGFQEMQPRVFAGLFPVDAEDYPDLREALDK
LRLNDAALRFEPESSEAMGFGFRCGFLGMLHMEIVQERLEREYNLDLISTAPTVVYEVLKTDGTVINMDNPAKLPQLNLV
QEIREPIIRANVLTPEEYIGNIIKLCEEKRGTQIGINYLGSQVQISYELPMAEVVLDFFDKLKSVSRGYASLDYHFVRFD
AGPFVRVDVLINGDKVDALSLIVHRGHADRRGRELCEKMKDLIPRQMFDVAIQAAIGSQIISRSTVKAMRKNVLAKCYGG
DVSRKKKLLEKQKEGKKRMKQVGRVEIPQEAFLAVLQMDK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=607, Percent_Identity=48.5996705107084, Blast_Score=609, Evalue=1e-174,
Organism=Homo sapiens, GI4503483, Length=148, Percent_Identity=42.5675675675676, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=45.8646616541353, Blast_Score=111, Evalue=3e-24,
Organism=Homo sapiens, GI25306283, Length=134, Percent_Identity=39.5522388059701, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI25306287, Length=134, Percent_Identity=41.7910447761194, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI19923640, Length=134, Percent_Identity=39.5522388059701, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=44.5454545454545, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=44.5454545454545, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=44.5454545454545, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI18390331, Length=158, Percent_Identity=34.8101265822785, Blast_Score=90, Evalue=8e-18,
Organism=Homo sapiens, GI217272894, Length=139, Percent_Identity=35.9712230215827, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI217272892, Length=139, Percent_Identity=35.9712230215827, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI53729339, Length=267, Percent_Identity=28.0898876404494, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI53729337, Length=267, Percent_Identity=28.0898876404494, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI34147630, Length=131, Percent_Identity=36.6412213740458, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=69.7986577181208, Blast_Score=847, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=513, Percent_Identity=27.8752436647173, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=34.1935483870968, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1790835, Length=137, Percent_Identity=32.1167883211679, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1789559, Length=231, Percent_Identity=31.1688311688312, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789737, Length=134, Percent_Identity=34.3283582089552, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1790412, Length=134, Percent_Identity=34.3283582089552, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=611, Percent_Identity=40.5891980360065, Blast_Score=458, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17556745, Length=463, Percent_Identity=25.0539956803456, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=160, Percent_Identity=37.5, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=36.8421052631579, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=36.8421052631579, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=35.1724137931034, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=137, Percent_Identity=34.3065693430657, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI32566303, Length=307, Percent_Identity=28.9902280130293, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556456, Length=143, Percent_Identity=37.0629370629371, Blast_Score=68, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=603, Percent_Identity=46.4344941956882, Blast_Score=540, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6324707, Length=188, Percent_Identity=38.2978723404255, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6320593, Length=188, Percent_Identity=38.2978723404255, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6323098, Length=158, Percent_Identity=36.0759493670886, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=37.3913043478261, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324166, Length=144, Percent_Identity=40.2777777777778, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=271, Percent_Identity=29.8892988929889, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324550, Length=273, Percent_Identity=29.3040293040293, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI78706572, Length=600, Percent_Identity=44.6666666666667, Blast_Score=525, Evalue=1e-149,
Organism=Drosophila melanogaster, GI24585709, Length=191, Percent_Identity=34.0314136125654, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585711, Length=191, Percent_Identity=34.0314136125654, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585713, Length=191, Percent_Identity=34.0314136125654, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=43.8848920863309, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24582462, Length=184, Percent_Identity=32.0652173913043, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI221458488, Length=148, Percent_Identity=36.4864864864865, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21357743, Length=134, Percent_Identity=36.5671641791045, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI28572034, Length=225, Percent_Identity=28.4444444444444, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281363316, Length=149, Percent_Identity=32.2147651006711, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17864358, Length=149, Percent_Identity=32.2147651006711, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66330; Mature: 66199

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDSMRNIRNFSIIAHVDHGKSTLADRIIQLCGGLQAREMEAQVLDSNPIERERGITIK
CCCCHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHCCCEEE
AQSVSLPYTAKDGQTYHLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVAN
EEEEECCEEECCCCEEEEEEECCCCCCEEEHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
CYTAVEQGLEVVPVLNKIDLPTADVDRAKAEIEAVIGIDAEDAVAVSAKTGLNIDLVLEA
HHHHHHCCCEEEEHHHHCCCCCCCHHHHHHHEEHEEECCCCCCEEEECCCCCCHHHHHHH
IVHRIPPPKPRDTDKLQALIIDSWFDNYLGVVSLVRVMQGEIKPGSKILVMSTGRTHLVD
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHH
KVGVFTPKRKELPALGAGEVGWINASIKDVHGAPVGDTLTLAGDPAPHALPGFQEMQPRV
HHCCCCCCHHCCCCCCCCCCEEEECCHHHCCCCCCCCEEEECCCCCCCCCCCHHHHCCHH
FAGLFPVDAEDYPDLREALDKLRLNDAALRFEPESSEAMGFGFRCGFLGMLHMEIVQERL
HCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EREYNLDLISTAPTVVYEVLKTDGTVINMDNPAKLPQLNLVQEIREPIIRANVLTPEEYI
HHHCCCEEECCCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHH
GNIIKLCEEKRGTQIGINYLGSQVQISYELPMAEVVLDFFDKLKSVSRGYASLDYHFVRF
HHHHHHHHHHCCCEEEHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHCCEEEEEE
DAGPFVRVDVLINGDKVDALSLIVHRGHADRRGRELCEKMKDLIPRQMFDVAIQAAIGSQ
CCCCEEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IISRSTVKAMRKNVLAKCYGGDVSRKKKLLEKQKEGKKRMKQVGRVEIPQEAFLAVLQMD
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
K
C
>Mature Secondary Structure 
SSDSMRNIRNFSIIAHVDHGKSTLADRIIQLCGGLQAREMEAQVLDSNPIERERGITIK
CCCHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHCCCEEE
AQSVSLPYTAKDGQTYHLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVAN
EEEEECCEEECCCCEEEEEEECCCCCCEEEHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
CYTAVEQGLEVVPVLNKIDLPTADVDRAKAEIEAVIGIDAEDAVAVSAKTGLNIDLVLEA
HHHHHHCCCEEEEHHHHCCCCCCCHHHHHHHEEHEEECCCCCCEEEECCCCCCHHHHHHH
IVHRIPPPKPRDTDKLQALIIDSWFDNYLGVVSLVRVMQGEIKPGSKILVMSTGRTHLVD
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHH
KVGVFTPKRKELPALGAGEVGWINASIKDVHGAPVGDTLTLAGDPAPHALPGFQEMQPRV
HHCCCCCCHHCCCCCCCCCCEEEECCHHHCCCCCCCCEEEECCCCCCCCCCCHHHHCCHH
FAGLFPVDAEDYPDLREALDKLRLNDAALRFEPESSEAMGFGFRCGFLGMLHMEIVQERL
HCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EREYNLDLISTAPTVVYEVLKTDGTVINMDNPAKLPQLNLVQEIREPIIRANVLTPEEYI
HHHCCCEEECCCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHH
GNIIKLCEEKRGTQIGINYLGSQVQISYELPMAEVVLDFFDKLKSVSRGYASLDYHFVRF
HHHHHHHHHHCCCEEEHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHCCEEEEEE
DAGPFVRVDVLINGDKVDALSLIVHRGHADRRGRELCEKMKDLIPRQMFDVAIQAAIGSQ
CCCCEEEEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IISRSTVKAMRKNVLAKCYGGDVSRKKKLLEKQKEGKKRMKQVGRVEIPQEAFLAVLQMD
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA