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The map label for this gene is purT

Identifier: 21241991

GI number: 21241991

Start: 1413589

End: 1414791

Strand: Direct

Name: purT

Synonym: XAC1237

Alternate gene names: 21241991

Gene position: 1413589-1414791 (Clockwise)

Preceding gene: 21241986

Following gene: 21241994

Centisome position: 27.31

GC content: 67.33

Gene sequence:

>1203_bases
ATGACCACTCTCGGCACGCCGTTATCGCCTTCCGCCACCCGCGTGCTGTTGTTGGGCTCGGGCGAACTGGGCAAGGAAGT
GGCGATCGAGCTGCAGCGTTTCGGTGTGGAAGTGATCGCGGCCGACCGCTATGCCAATGCACCGGCGATGCAGGTGGCGC
ACCGCTCGCATGTGCTGGACATGCTCGACCCCATTGCGTTGCGCACGCTGATCGCCAATGAGCGGCCGCATCTGATCGTG
CCGGAGATCGAAGCGATCCATACCGAAGCCCTGGTGGCGCTGGAACGCGAGCAAGGCCAGAAGGTCATCCCGACGGCGCG
TGCCGCGCGCCTGACCATGGACCGCGAAGGCATTCGCCGCCTGGCAGCCGAAACGCTGGGCCTGCCGACCTCGCCGTATC
GCTTTGTCGATACCGTCGAGGAGTATCGCGATGCAATTGCCGCGGTGGGCTTGCCATGCGTGGTCAAGCCGGTGATGTCG
TCCTCGGGCAAGGGCCAGAGCACCCTGCGCAGCGAGGCCGATATCGATGCGGCATGGGAGTATGCGCAGACCGGCGGACG
TGCCGGTGCCGGTCGCTGCATCGTCGAAGGCTTCATCGATTTCGATTACGAAATTACCTTGCTCACCGTGCGCCATGCCG
GCGGCACCTCGTACTGCGATCCCATCGGGCATTGGCAACAGGACGGCGACTACCGCGAAAGCTGGCAGCCGCAGCCGATG
TCGGCCGCTGCGTTGCGCCGCTCGCAGCAGATCGCCAAGGCCATCACCGACGATCTCGGCGGCCGGGGCCTGTTCGGTGT
GGAGTTGTTCGTCAAGGGCGACGAGGTGTGGTTCAGCGAAGTCTCGCCGCGCCCGCACGACACCGGCCTGGTCACGCTGG
TCTCGCAGGAACTGAGCGAATTCGCGCTGCATGCCCGCGCCATTCTCGGCCTGCCGGTGGGCGCGCAGGACGGCGGCGTG
ATCCGTCAGGCCGGCCCTTCGGCATCGTGCGCGTTGCTGGCGCACGGCAATGGCGTGCCGGCGTTCGACAACGTGGCCGA
AGCACTGCGGGACCCTGACACCGCACTGCGCCTGTTCGGCAAGCCCCGCGTGGACGGCCATCGCCGCGTCGGCGTCACCC
TGGCGCGCGCGGACAGCGTCGATGCTGCGCGTGAGAAGGCGCGCGTCGCCGCCGCGGCGTTGACGATCCAGCTGCAGGCC
TGA

Upstream 100 bases:

>100_bases
AAAAAGATATCAATGTGATATCGGTTAAACAATGGCCGTTCGTCGGAGTGGGAGCGGGCAACCGTTAGAATGGCGGCCCC
TCTCGCTCTGGACCGCGCTC

Downstream 100 bases:

>100_bases
GCGGCGTACGGCAGACGCAGTGCAGCGCATCGCTGCATTGCGTCGACGCGCCAGGGGCGCTCGGGATTTATGCGCACGTC
TTCTCGACGCTGCCCGACGC

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTTLGTPLSPSATRVLLLGSGELGKEVAIELQRFGVEVIAADRYANAPAMQVAHRSHVLDMLDPIALRTLIANERPHLIV
PEIEAIHTEALVALEREQGQKVIPTARAARLTMDREGIRRLAAETLGLPTSPYRFVDTVEEYRDAIAAVGLPCVVKPVMS
SSGKGQSTLRSEADIDAAWEYAQTGGRAGAGRCIVEGFIDFDYEITLLTVRHAGGTSYCDPIGHWQQDGDYRESWQPQPM
SAAALRRSQQIAKAITDDLGGRGLFGVELFVKGDEVWFSEVSPRPHDTGLVTLVSQELSEFALHARAILGLPVGAQDGGV
IRQAGPSASCALLAHGNGVPAFDNVAEALRDPDTALRLFGKPRVDGHRRVGVTLARADSVDAAREKARVAAAALTIQLQA

Sequences:

>Translated_400_residues
MTTLGTPLSPSATRVLLLGSGELGKEVAIELQRFGVEVIAADRYANAPAMQVAHRSHVLDMLDPIALRTLIANERPHLIV
PEIEAIHTEALVALEREQGQKVIPTARAARLTMDREGIRRLAAETLGLPTSPYRFVDTVEEYRDAIAAVGLPCVVKPVMS
SSGKGQSTLRSEADIDAAWEYAQTGGRAGAGRCIVEGFIDFDYEITLLTVRHAGGTSYCDPIGHWQQDGDYRESWQPQPM
SAAALRRSQQIAKAITDDLGGRGLFGVELFVKGDEVWFSEVSPRPHDTGLVTLVSQELSEFALHARAILGLPVGAQDGGV
IRQAGPSASCALLAHGNGVPAFDNVAEALRDPDTALRLFGKPRVDGHRRVGVTLARADSVDAAREKARVAAAALTIQLQA
>Mature_399_residues
TTLGTPLSPSATRVLLLGSGELGKEVAIELQRFGVEVIAADRYANAPAMQVAHRSHVLDMLDPIALRTLIANERPHLIVP
EIEAIHTEALVALEREQGQKVIPTARAARLTMDREGIRRLAAETLGLPTSPYRFVDTVEEYRDAIAAVGLPCVVKPVMSS
SGKGQSTLRSEADIDAAWEYAQTGGRAGAGRCIVEGFIDFDYEITLLTVRHAGGTSYCDPIGHWQQDGDYRESWQPQPMS
AAALRRSQQIAKAITDDLGGRGLFGVELFVKGDEVWFSEVSPRPHDTGLVTLVSQELSEFALHARAILGLPVGAQDGGVI
RQAGPSASCALLAHGNGVPAFDNVAEALRDPDTALRLFGKPRVDGHRRVGVTLARADSVDAAREKARVAAAALTIQLQA

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=398, Percent_Identity=63.8190954773869, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI1786733, Length=192, Percent_Identity=30.2083333333333, Blast_Score=75, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6324702, Length=308, Percent_Identity=23.7012987012987, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_XANAC (Q8PN36)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641573.1
- HSSP:   P33221
- ProteinModelPortal:   Q8PN36
- SMR:   Q8PN36
- GeneID:   1155308
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC1237
- NMPDR:   fig|190486.1.peg.1217
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- ProtClustDB:   PRK09288
- BioCyc:   XAXO190486:XAC1237-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42899; Mature: 42768

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 82-82 BINDING 115-115 BINDING 156-156 BINDING 204-204 BINDING 268-268 BINDING 280-280 BINDING 287-287 BINDING 361-361

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLGTPLSPSATRVLLLGSGELGKEVAIELQRFGVEVIAADRYANAPAMQVAHRSHVLD
CCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHH
MLDPIALRTLIANERPHLIVPEIEAIHTEALVALEREQGQKVIPTARAARLTMDREGIRR
HHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHCHHHHHH
LAAETLGLPTSPYRFVDTVEEYRDAIAAVGLPCVVKPVMSSSGKGQSTLRSEADIDAAWE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHCCCHHHHHH
YAQTGGRAGAGRCIVEGFIDFDYEITLLTVRHAGGTSYCDPIGHWQQDGDYRESWQPQPM
HHHCCCCCCCCCEEEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCC
SAAALRRSQQIAKAITDDLGGRGLFGVELFVKGDEVWFSEVSPRPHDTGLVTLVSQELSE
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHH
FALHARAILGLPVGAQDGGVIRQAGPSASCALLAHGNGVPAFDNVAEALRDPDTALRLFG
HHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHCC
KPRVDGHRRVGVTLARADSVDAAREKARVAAAALTIQLQA
CCCCCCCCEEEEEEEECCCHHHHHHHHHHEEEEEEEEEEC
>Mature Secondary Structure 
TTLGTPLSPSATRVLLLGSGELGKEVAIELQRFGVEVIAADRYANAPAMQVAHRSHVLD
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHH
MLDPIALRTLIANERPHLIVPEIEAIHTEALVALEREQGQKVIPTARAARLTMDREGIRR
HHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHCHHHHHH
LAAETLGLPTSPYRFVDTVEEYRDAIAAVGLPCVVKPVMSSSGKGQSTLRSEADIDAAWE
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHCCCHHHHHH
YAQTGGRAGAGRCIVEGFIDFDYEITLLTVRHAGGTSYCDPIGHWQQDGDYRESWQPQPM
HHHCCCCCCCCCEEEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCC
SAAALRRSQQIAKAITDDLGGRGLFGVELFVKGDEVWFSEVSPRPHDTGLVTLVSQELSE
HHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHH
FALHARAILGLPVGAQDGGVIRQAGPSASCALLAHGNGVPAFDNVAEALRDPDTALRLFG
HHHHHHHHHCCCCCCCCCCEEEECCCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHCC
KPRVDGHRRVGVTLARADSVDAAREKARVAAAALTIQLQA
CCCCCCCCEEEEEEEECCCHHHHHHHHHHEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217