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The map label for this gene is purA [H]

Identifier: 21241912

GI number: 21241912

Start: 1318709

End: 1320001

Strand: Direct

Name: purA [H]

Synonym: XAC1158

Alternate gene names: 21241912

Gene position: 1318709-1320001 (Clockwise)

Preceding gene: 21241911

Following gene: 21241913

Centisome position: 25.48

GC content: 65.82

Gene sequence:

>1293_bases
ATGGGTCAGTCAGTTGTCGTGCTCGGTGCCCAGTGGGGCGATGAAGGCAAAGGCAAGATCGTCGATCTGCTCACCGAGGA
AATCGGTGCGGTGGTCCGCTTCCAGGGCGGCCACAACGCCGGCCACACGCTTGTCATCAACGGCAAGAAGACCGTCTTGC
ATCTGATTCCGTCCGGCATCCTGCGCGACGACGCGCTGTGCCTGATCGGCAATGGCGTGGTGATCTCCCCGGCCGCGTTG
ATCAAGGAAATCAGCGAGCTGGAAGACGCCGGCGTGGAAGTGCGTTCGCGCCTGAAGATCTCCCCGGCTGCGCCGTTGAT
CATGCCCTACCACATCGCGCTGGATCAGGCGCGCGAGAAGGCGGCCGGCGGCAAGGCCATCGGCACCACCGGCCGCGGCA
TCGGCCCGGCCTACGAAGACAAGGTGGCGCGTCGCGGCATCCGCATCGCCGACCTGCATTACCCGCCGCAGCTGGAAGAG
TTGCTGCGCACTGCGCTGGACTACCACAACTTCGTGCTGACCAAGTACCTGGGCGTGGAAGCGGTCGATTTCCAGAAGAC
CTACGACGAGGCGCTGGCCTTCGGCGAGTACGTGCAGCCGATGAAGTCGGACGTGGCCGGGATCCTGCACGACCTGCGCA
AGCAGGGTAAGCGCGTGTTGTTCGAAGGCGCGCAGGGTGCGTTGCTGGACATCGACCACGGCACCTATCCGTACGTCACC
AGTTCCAACACCACCGTGGGCGGCGCATTGGCCGGTACCGGCGTTGGCGCCGATGCGATCGACTACGTACTCGGCATTGC
CAAAGCATATGCCACGCGCGTCGGCGGCGGCCCGTTCCCGACCGAACTGGACGACGAAGTGGGCCAGGGTATCCGCGACC
GCGGTGCCGAGTACGGCGCGTCCACCGGTCGCCCGCGTCGCTGCGGCTGGATGGATATCGTGGCGCTCAAGCGTGCGGTG
GCCATCAACGGCATTTCCGGCCTGTGCATCACCAAGCTCGACGTGCTCGACGGCATGGAGAAGCTGAAGATCTGCATCGC
CTACGAATATCACGGCAAGCGCACCGAGTACGCGCCGCTGGACGCGCAGGGCTGGGAAGAGTGCACCCCCGTGTACCTGG
AGTTCCCGGGCTGGAGCGAAAACACCCACGGCATCACCGTGTGGGACGACCTGCCGCCGGCCGCCCGCGCCTACCTGCGC
GCGCTGGAGGAACTGGCCGGCTGCCCGATCTCGATCGTTTCCACCGGCCCGGACCGCGACCACACCATGGTGCTGCAGGA
CCCGTTCGCCTGA

Upstream 100 bases:

>100_bases
GCTCCCCGATGGAGTCGCGCGCAGGGTGTCGCGGCCAGCCCTGCAGCGGTCCCATCCCGCGGCCCCGATGGCCGCAGCAA
AACGTAGGAGTTCACCCGTC

Downstream 100 bases:

>100_bases
TCGGCTGATGCGTGATTGAACCGACGAAAGCCCTGTGAAAGCGGGGCTTTTGTTTGCAGAACGCCATTGCGTTGCCTCAT
CCACGCGCCCCGCACCTTCC

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MGQSVVVLGAQWGDEGKGKIVDLLTEEIGAVVRFQGGHNAGHTLVINGKKTVLHLIPSGILRDDALCLIGNGVVISPAAL
IKEISELEDAGVEVRSRLKISPAAPLIMPYHIALDQAREKAAGGKAIGTTGRGIGPAYEDKVARRGIRIADLHYPPQLEE
LLRTALDYHNFVLTKYLGVEAVDFQKTYDEALAFGEYVQPMKSDVAGILHDLRKQGKRVLFEGAQGALLDIDHGTYPYVT
SSNTTVGGALAGTGVGADAIDYVLGIAKAYATRVGGGPFPTELDDEVGQGIRDRGAEYGASTGRPRRCGWMDIVALKRAV
AINGISGLCITKLDVLDGMEKLKICIAYEYHGKRTEYAPLDAQGWEECTPVYLEFPGWSENTHGITVWDDLPPAARAYLR
ALEELAGCPISIVSTGPDRDHTMVLQDPFA

Sequences:

>Translated_430_residues
MGQSVVVLGAQWGDEGKGKIVDLLTEEIGAVVRFQGGHNAGHTLVINGKKTVLHLIPSGILRDDALCLIGNGVVISPAAL
IKEISELEDAGVEVRSRLKISPAAPLIMPYHIALDQAREKAAGGKAIGTTGRGIGPAYEDKVARRGIRIADLHYPPQLEE
LLRTALDYHNFVLTKYLGVEAVDFQKTYDEALAFGEYVQPMKSDVAGILHDLRKQGKRVLFEGAQGALLDIDHGTYPYVT
SSNTTVGGALAGTGVGADAIDYVLGIAKAYATRVGGGPFPTELDDEVGQGIRDRGAEYGASTGRPRRCGWMDIVALKRAV
AINGISGLCITKLDVLDGMEKLKICIAYEYHGKRTEYAPLDAQGWEECTPVYLEFPGWSENTHGITVWDDLPPAARAYLR
ALEELAGCPISIVSTGPDRDHTMVLQDPFA
>Mature_429_residues
GQSVVVLGAQWGDEGKGKIVDLLTEEIGAVVRFQGGHNAGHTLVINGKKTVLHLIPSGILRDDALCLIGNGVVISPAALI
KEISELEDAGVEVRSRLKISPAAPLIMPYHIALDQAREKAAGGKAIGTTGRGIGPAYEDKVARRGIRIADLHYPPQLEEL
LRTALDYHNFVLTKYLGVEAVDFQKTYDEALAFGEYVQPMKSDVAGILHDLRKQGKRVLFEGAQGALLDIDHGTYPYVTS
SNTTVGGALAGTGVGADAIDYVLGIAKAYATRVGGGPFPTELDDEVGQGIRDRGAEYGASTGRPRRCGWMDIVALKRAVA
INGISGLCITKLDVLDGMEKLKICIAYEYHGKRTEYAPLDAQGWEECTPVYLEFPGWSENTHGITVWDDLPPAARAYLRA
LEELAGCPISIVSTGPDRDHTMVLQDPFA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=428, Percent_Identity=42.0560747663551, Blast_Score=330, Evalue=1e-90,
Organism=Homo sapiens, GI34577063, Length=430, Percent_Identity=40.6976744186046, Blast_Score=305, Evalue=7e-83,
Organism=Homo sapiens, GI40316944, Length=398, Percent_Identity=40.2010050251256, Blast_Score=291, Evalue=9e-79,
Organism=Escherichia coli, GI1790620, Length=430, Percent_Identity=60.9302325581395, Blast_Score=543, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI25146553, Length=434, Percent_Identity=43.0875576036866, Blast_Score=340, Evalue=8e-94,
Organism=Caenorhabditis elegans, GI25146555, Length=434, Percent_Identity=43.0875576036866, Blast_Score=340, Evalue=9e-94,
Organism=Saccharomyces cerevisiae, GI6324109, Length=428, Percent_Identity=42.7570093457944, Blast_Score=340, Evalue=3e-94,
Organism=Drosophila melanogaster, GI21356033, Length=418, Percent_Identity=40.9090909090909, Blast_Score=321, Evalue=5e-88,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46200; Mature: 46069

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQSVVVLGAQWGDEGKGKIVDLLTEEIGAVVRFQGGHNAGHTLVINGKKTVLHLIPSGI
CCCCEEEEEECCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCEEEECCCCEEEEECCCCC
LRDDALCLIGNGVVISPAALIKEISELEDAGVEVRSRLKISPAAPLIMPYHIALDQAREK
CCCCCEEEECCCEEECHHHHHHHHHHHHHCCHHHHHCEEECCCCCCEEEHHHHHHHHHHH
AAGGKAIGTTGRGIGPAYEDKVARRGIRIADLHYPPQLEELLRTALDYHNFVLTKYLGVE
HCCCCEECCCCCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCE
AVDFQKTYDEALAFGEYVQPMKSDVAGILHDLRKQGKRVLFEGAQGALLDIDHGTYPYVT
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEE
SSNTTVGGALAGTGVGADAIDYVLGIAKAYATRVGGGPFPTELDDEVGQGIRDRGAEYGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHCCCCCCC
STGRPRRCGWMDIVALKRAVAINGISGLCITKLDVLDGMEKLKICIAYEYHGKRTEYAPL
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHEEEEEEEEECCCCCCCCCC
DAQGWEECTPVYLEFPGWSENTHGITVWDDLPPAARAYLRALEELAGCPISIVSTGPDRD
CCCCCCCCCCEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCC
HTMVLQDPFA
CEEEEECCCC
>Mature Secondary Structure 
GQSVVVLGAQWGDEGKGKIVDLLTEEIGAVVRFQGGHNAGHTLVINGKKTVLHLIPSGI
CCCEEEEEECCCCCCCCHHHHHHHHHHCEEEEECCCCCCCCEEEECCCCEEEEECCCCC
LRDDALCLIGNGVVISPAALIKEISELEDAGVEVRSRLKISPAAPLIMPYHIALDQAREK
CCCCCEEEECCCEEECHHHHHHHHHHHHHCCHHHHHCEEECCCCCCEEEHHHHHHHHHHH
AAGGKAIGTTGRGIGPAYEDKVARRGIRIADLHYPPQLEELLRTALDYHNFVLTKYLGVE
HCCCCEECCCCCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCE
AVDFQKTYDEALAFGEYVQPMKSDVAGILHDLRKQGKRVLFEGAQGALLDIDHGTYPYVT
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEE
SSNTTVGGALAGTGVGADAIDYVLGIAKAYATRVGGGPFPTELDDEVGQGIRDRGAEYGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHCCCCCCC
STGRPRRCGWMDIVALKRAVAINGISGLCITKLDVLDGMEKLKICIAYEYHGKRTEYAPL
CCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHEEEEEEEEECCCCCCCCCC
DAQGWEECTPVYLEFPGWSENTHGITVWDDLPPAARAYLRALEELAGCPISIVSTGPDRD
CCCCCCCCCCEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCEEEEECCCCCC
HTMVLQDPFA
CEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA