Click here to switch to the map view.

The map label for this gene is dniR [H]

Identifier: 21241843

GI number: 21241843

Start: 1241249

End: 1242427

Strand: Reverse

Name: dniR [H]

Synonym: XAC1086

Alternate gene names: 21241843

Gene position: 1242427-1241249 (Counterclockwise)

Preceding gene: 21241844

Following gene: 21241836

Centisome position: 24.01

GC content: 66.84

Gene sequence:

>1179_bases
ATGAGGCGCCTGGTTTTGGCAGTGCTGGCCGCGTGTTCGGCCATTGCACCGGCAGCCGCATCGCCCAACGACGCAAGCTC
CGCCTCCGCAGGTGGCCAGGCCGCGCCGGCCAGCGCGCCAGGCCTCCGCAACGGGCGCGAGATTTTTTCGGCCTTCCTCG
ACGGACGCGCCGACCCGGGCTGCGACAGCGCCCATAGCGATGCCCGCTGGGAACAGCATTTTTCGCGCGCCCCGGCACGT
TTGGCAGACGATGCGCAGGATGTCCTGCCGCTATTCGGCTATGTCGTCGATGAATTGCGCAAGGCCGACATGCCGACCGA
ATTTGCGCTGATTCCGTTCGTCGAAAGCGGCTACCGCCCCGGGGCTCGCAACGGCAGCGGCCCTGCAGGCTTGTGGCAGT
TCATCGCAACCACCGCGCGCAACCACCAGGTGCCGGTCGGCGCGCAGTACGACGGACGCCTGTCGGCAGTGGATTCCACC
ACTGCGGCGGTACGCTATCTCAAAACCCTTTACGGCATGTTCGGCGGAGACTGGCGCCTGGCCCTGATGGCCTACAACGC
CGGCGAGTACCGCGTGCTGCAGGCGATGCGCAGCGCCGGGATGAACGCGCAAAACGTGCGTCCGTCCGAATTGCCGGGCA
TGTCCAAGGTCACCTACGACTATGTGGAAAAACTGCACGCGCTTGCCTGCGTGCTCGATCACGCGCAGCAGCAGGGCAAC
CTGCTGACCTCGCTGGACCGTCCGGTGCCTGTCCTCACCGAACATGCGTTACCGGCAGGCACCAGCCTCAACACCTGGGC
TGGACAGCGCGCGATCGAGCCGCAATTGCTCGCGCGCCTGAATCCCGCCCTCGCTGGCGCACGCGCCGCACCGCGCGGTG
TGTACGTGCTCGCACCGCTTGCGCCGCCGCAGCCCGGTGCAAATAGCGCCATGGTCGCATCTGCGGAGATGGAAACTGGC
GCCGCCAGCAGCACTGCCGCGCCCACAGCCGTCGCCGCTGCAGCCAGCAAAGCGTCGACCTCGCCAGCACGCACCCACAC
CGTGCGCAATGGCGAATCGGCCTGGGCGATCGCACGCCGCTATGGCATCACCGTCACGACGCTGCTCGCCACCAACGGCC
TGGACAAAAGCGCCATTCTGAAGCCTGGCATGGTGTTGTCGTTCGACGACTCGCCCTGA

Upstream 100 bases:

>100_bases
CCGAGATCCGCGCTGCAGTGGCACCACGCGCAGCCAGCGCACTTTCCTCTGAAGTGGATATTTTTGCCGAACTTCGGCGC
TGGAAAGACGAATTTCGGGC

Downstream 100 bases:

>100_bases
GCGATGCGCCGAGGTGCGGCGCGACCTTGGACGCTGTCGTACGCGCAGGCAGGCGGCCGCCAGCATGATCGCGTCGATCC
TGAACTTCGCTCACCAACCC

Product: murein hydrolase D

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MRRLVLAVLAACSAIAPAAASPNDASSASAGGQAAPASAPGLRNGREIFSAFLDGRADPGCDSAHSDARWEQHFSRAPAR
LADDAQDVLPLFGYVVDELRKADMPTEFALIPFVESGYRPGARNGSGPAGLWQFIATTARNHQVPVGAQYDGRLSAVDST
TAAVRYLKTLYGMFGGDWRLALMAYNAGEYRVLQAMRSAGMNAQNVRPSELPGMSKVTYDYVEKLHALACVLDHAQQQGN
LLTSLDRPVPVLTEHALPAGTSLNTWAGQRAIEPQLLARLNPALAGARAAPRGVYVLAPLAPPQPGANSAMVASAEMETG
AASSTAAPTAVAAAASKASTSPARTHTVRNGESAWAIARRYGITVTTLLATNGLDKSAILKPGMVLSFDDSP

Sequences:

>Translated_392_residues
MRRLVLAVLAACSAIAPAAASPNDASSASAGGQAAPASAPGLRNGREIFSAFLDGRADPGCDSAHSDARWEQHFSRAPAR
LADDAQDVLPLFGYVVDELRKADMPTEFALIPFVESGYRPGARNGSGPAGLWQFIATTARNHQVPVGAQYDGRLSAVDST
TAAVRYLKTLYGMFGGDWRLALMAYNAGEYRVLQAMRSAGMNAQNVRPSELPGMSKVTYDYVEKLHALACVLDHAQQQGN
LLTSLDRPVPVLTEHALPAGTSLNTWAGQRAIEPQLLARLNPALAGARAAPRGVYVLAPLAPPQPGANSAMVASAEMETG
AASSTAAPTAVAAAASKASTSPARTHTVRNGESAWAIARRYGITVTTLLATNGLDKSAILKPGMVLSFDDSP
>Mature_392_residues
MRRLVLAVLAACSAIAPAAASPNDASSASAGGQAAPASAPGLRNGREIFSAFLDGRADPGCDSAHSDARWEQHFSRAPAR
LADDAQDVLPLFGYVVDELRKADMPTEFALIPFVESGYRPGARNGSGPAGLWQFIATTARNHQVPVGAQYDGRLSAVDST
TAAVRYLKTLYGMFGGDWRLALMAYNAGEYRVLQAMRSAGMNAQNVRPSELPGMSKVTYDYVEKLHALACVLDHAQQQGN
LLTSLDRPVPVLTEHALPAGTSLNTWAGQRAIEPQLLARLNPALAGARAAPRGVYVLAPLAPPQPGANSAMVASAEMETG
AASSTAAPTAVAAAASKASTSPARTHTVRNGESAWAIARRYGITVTTLLATNGLDKSAILKPGMVLSFDDSP

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=321, Percent_Identity=30.2180685358255, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 40975; Mature: 40975

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLVLAVLAACSAIAPAAASPNDASSASAGGQAAPASAPGLRNGREIFSAFLDGRADPG
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
CDSAHSDARWEQHFSRAPARLADDAQDVLPLFGYVVDELRKADMPTEFALIPFVESGYRP
CCCCCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHCCCCC
GARNGSGPAGLWQFIATTARNHQVPVGAQYDGRLSAVDSTTAAVRYLKTLYGMFGGDWRL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEE
ALMAYNAGEYRVLQAMRSAGMNAQNVRPSELPGMSKVTYDYVEKLHALACVLDHAQQQGN
EEEEECCCHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
LLTSLDRPVPVLTEHALPAGTSLNTWAGQRAIEPQLLARLNPALAGARAAPRGVYVLAPL
CHHHCCCCCCHHHHCCCCCCCCCCHHCCCCCCCHHHHHHHCHHHHCCCCCCCCEEEEEEC
APPQPGANSAMVASAEMETGAASSTAAPTAVAAAASKASTSPARTHTVRNGESAWAIARR
CCCCCCCCCEEEEECHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHEECCCCHHHHHHHH
YGITVTTLLATNGLDKSAILKPGMVLSFDDSP
HCCEEEEEEHHCCCCHHHHCCCCEEEEECCCC
>Mature Secondary Structure
MRRLVLAVLAACSAIAPAAASPNDASSASAGGQAAPASAPGLRNGREIFSAFLDGRADPG
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
CDSAHSDARWEQHFSRAPARLADDAQDVLPLFGYVVDELRKADMPTEFALIPFVESGYRP
CCCCCCHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHCCCCC
GARNGSGPAGLWQFIATTARNHQVPVGAQYDGRLSAVDSTTAAVRYLKTLYGMFGGDWRL
CCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEE
ALMAYNAGEYRVLQAMRSAGMNAQNVRPSELPGMSKVTYDYVEKLHALACVLDHAQQQGN
EEEEECCCHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
LLTSLDRPVPVLTEHALPAGTSLNTWAGQRAIEPQLLARLNPALAGARAAPRGVYVLAPL
CHHHCCCCCCHHHHCCCCCCCCCCHHCCCCCCCHHHHHHHCHHHHCCCCCCCCEEEEEEC
APPQPGANSAMVASAEMETGAASSTAAPTAVAAAASKASTSPARTHTVRNGESAWAIARR
CCCCCCCCCEEEEECHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHEECCCCHHHHHHHH
YGITVTTLLATNGLDKSAILKPGMVLSFDDSP
HCCEEEEEEHHCCCCHHHHCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]