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The map label for this gene is insF1 [C]

Identifier: 21241833

GI number: 21241833

Start: 1227453

End: 1228271

Strand: Direct

Name: insF1 [C]

Synonym: XAC1071

Alternate gene names: 21241833

Gene position: 1227453-1228271 (Clockwise)

Preceding gene: 21241832

Following gene: 21241837

Centisome position: 23.72

GC content: 61.9

Gene sequence:

>819_bases
ATGTGCCGGGTTTTGCGGGTGAACCGGGCTGGATACTACGCGTGGCTAAAGTCGCCCGACAGTGAACGCGCCAAGGAAGA
CGAACGCCTGCTGGGGCTGATCAAGCACCACTGGTTGGCCAGCGGCAGTGTCTATGGGCATCGCAAGATTGCCAAGGATC
TACGCGATCTGGGTGAGCGTTGCAGTCGCCATCGGGTGCATCGATTGATGCGCACCGAGGGACTGCGTGCCCAGGTGGGC
TATGGCCGCAAGCCGCGCTTCCATGGCGGAACGCCGTGCAAGGCGGCAGCCAACCTGCTGGATCGACAGTTCGACGTGAC
CGAGCCGGACACGGCCTGGGCGAGCGACTTCACCTTCATCCGTACGCATGAAGGCTGGATGTACCTGGCTGTGGTGATCG
ATCTGTTTTCCAGGCAGGTCGTCGGCTGGGCGATGCGCGATCGAGCCGATACCGAGTTGGTCGTGCAGGCCTTGCTGTCG
GCGGTGTGGCGGCGCAAACCCAGCGCTGGTTGCCTGGTTCATTCGGACCAGGGGTCTGTCTACACCAGCGATGACTGGCG
CAGTTTCCTGGCGTCCCATGGCTTGGTGTGCAGCATGAGTCGGCGTGGCAACTGCCATGACAACGCACCGGTGGAGAGCT
TCTTTGGTCTGCTCAAACGCGAGCGGATCAGGCGGCGGATCTACCCCACCAAGGACGCCGCACGCGCCGAGGTGTTCGAC
TACATCGAGATGTTCTACAACCCACAACGCCGTCATGGTTCAACTGGCGACCTGTCCCCTGTAGAGTTTGAACGGCGCTA
CGCGCAACGAGGGTCTTGA

Upstream 100 bases:

>100_bases
AGCGCGACATCCTAAAAAAGGCCGCCGCGTACTTTGCCAAGGGGTAAAGGCAAAGTACCTCTTCATGCAAGTCCACCGCG
AAGAATTCAGGGTGTGCGCG

Downstream 100 bases:

>100_bases
GTGTCTACGGAACCCTGGGCGTATCACGCAGCTTCTTGTAGTTCACGTCCTGTGCGATGGCAGAGCCGACAGAGCGCGCA
ACGGCGGTCAACTTAGCCTG

Product: ISxac3 transposase

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MCRVLRVNRAGYYAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHRKIAKDLRDLGERCSRHRVHRLMRTEGLRAQVG
YGRKPRFHGGTPCKAAANLLDRQFDVTEPDTAWASDFTFIRTHEGWMYLAVVIDLFSRQVVGWAMRDRADTELVVQALLS
AVWRRKPSAGCLVHSDQGSVYTSDDWRSFLASHGLVCSMSRRGNCHDNAPVESFFGLLKRERIRRRIYPTKDAARAEVFD
YIEMFYNPQRRHGSTGDLSPVEFERRYAQRGS

Sequences:

>Translated_272_residues
MCRVLRVNRAGYYAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHRKIAKDLRDLGERCSRHRVHRLMRTEGLRAQVG
YGRKPRFHGGTPCKAAANLLDRQFDVTEPDTAWASDFTFIRTHEGWMYLAVVIDLFSRQVVGWAMRDRADTELVVQALLS
AVWRRKPSAGCLVHSDQGSVYTSDDWRSFLASHGLVCSMSRRGNCHDNAPVESFFGLLKRERIRRRIYPTKDAARAEVFD
YIEMFYNPQRRHGSTGDLSPVEFERRYAQRGS
>Mature_272_residues
MCRVLRVNRAGYYAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHRKIAKDLRDLGERCSRHRVHRLMRTEGLRAQVG
YGRKPRFHGGTPCKAAANLLDRQFDVTEPDTAWASDFTFIRTHEGWMYLAVVIDLFSRQVVGWAMRDRADTELVVQALLS
AVWRRKPSAGCLVHSDQGSVYTSDDWRSFLASHGLVCSMSRRGNCHDNAPVESFFGLLKRERIRRRIYPTKDAARAEVFD
YIEMFYNPQRRHGSTGDLSPVEFERRYAQRGS

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=228, Percent_Identity=35.5263157894737, Blast_Score=125, Evalue=3e-29,
Organism=Escherichia coli, GI1788405, Length=268, Percent_Identity=40.2985074626866, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1787263, Length=268, Percent_Identity=40.2985074626866, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1786753, Length=268, Percent_Identity=40.2985074626866, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1786570, Length=268, Percent_Identity=40.2985074626866, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1786490, Length=268, Percent_Identity=40.2985074626866, Blast_Score=191, Evalue=6e-50,
Organism=Escherichia coli, GI1789981, Length=219, Percent_Identity=34.2465753424658, Blast_Score=129, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 31419; Mature: 31419

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRVLRVNRAGYYAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHRKIAKDLRDLGER
CCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
CSRHRVHRLMRTEGLRAQVGYGRKPRFHGGTPCKAAANLLDRQFDVTEPDTAWASDFTFI
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHCCCEEE
RTHEGWMYLAVVIDLFSRQVVGWAMRDRADTELVVQALLSAVWRRKPSAGCLVHSDQGSV
EECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCE
YTSDDWRSFLASHGLVCSMSRRGNCHDNAPVESFFGLLKRERIRRRIYPTKDAARAEVFD
EECHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
YIEMFYNPQRRHGSTGDLSPVEFERRYAQRGS
HHHHHHCHHHCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MCRVLRVNRAGYYAWLKSPDSERAKEDERLLGLIKHHWLASGSVYGHRKIAKDLRDLGER
CCEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
CSRHRVHRLMRTEGLRAQVGYGRKPRFHGGTPCKAAANLLDRQFDVTEPDTAWASDFTFI
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHCCCEEE
RTHEGWMYLAVVIDLFSRQVVGWAMRDRADTELVVQALLSAVWRRKPSAGCLVHSDQGSV
EECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCE
YTSDDWRSFLASHGLVCSMSRRGNCHDNAPVESFFGLLKRERIRRRIYPTKDAARAEVFD
EECHHHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
YIEMFYNPQRRHGSTGDLSPVEFERRYAQRGS
HHHHHHCHHHCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]