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The map label for this gene is ostA [H]

Identifier: 21241636

GI number: 21241636

Start: 1027583

End: 1030036

Strand: Reverse

Name: ostA [H]

Synonym: XAC0866

Alternate gene names: 21241636

Gene position: 1030036-1027583 (Counterclockwise)

Preceding gene: 21241637

Following gene: 21241635

Centisome position: 19.9

GC content: 62.75

Gene sequence:

>2454_bases
TTGAACCCACGCGTGCGCCGAGCTCTCCGCCTGCTGCCCCTGCCTTTGAGCATCGCCATCTGCCTCCCGGCCATGGCTGC
CGACAAGCCGTTGAACTGGGGACTGTGCCCGGCCGTAGACCCGCTACCCGGCTTCGACGGCGCCCCTGCCGCCGACCCCA
AGGCGGCCGAAATGCGCCAGCAGTTGCCGACCGACATCGAAGGCGACCAGCTCTCCGGCACCAGCACCACCCCGCAATAC
CAAGGCAATGTTGCGTTGAAGCGCGGCGACCAGTTCCTGGGTGCCGACAACCTGCGCATGGACACCGAAACGGGCAACTA
CATCGCCGAAGGCAATGTCCGCTACCAGGACACCTCTTTCCGCATGGTCGCCGACCGCGCCGAAGGCAATCAGGATACCG
ACACCCACAAGGTCACCAACATCCAGTACCAGTTGGTGGAGCGCCGCGGCAATGGCGACGCCGAATCGGTGGACCTGCAG
GGCCAGGTCGGGCAAATGCATCGATCCACGTACACCACCTGCGACCCCTCGCAGCCGATCTGGCGCGTTCGCGCGCCGGA
GATCGACGTCGACAACGAGGAAGGTTTCGGCACTGCACGCAACGCGGTGCTGCAGATCGGCAAGGTGCCGGTGCTGTACT
TCCCGTGGTTCAAGTTCCCCATCGACGACCGCCGCCAGACCGGCCTGCTGTTTCCGCAATTCGGTTTGTCCGGTCGTAAC
GGCTTCGATTACCTGCAGCCGATCTATCTGAACCTGGCGCCGAACTACGACGCCACGTTGCTGCCGCGCTACATGAGCAA
GCGCGGTTTCATGTTCGGCACCGAATTCCGTTACCTGTACGAAGGCGGTCGCGGCGAAGTCACCGGCAATTACCTGCCCA
ACGACAAGTTGCGCGACAAGGACCGCGGCAGCGTGTTCTACAGCGGCTACCACAACGTCAACACCCACTGGCAGGCGCGC
AGCAGCATCTCCTGGGTCAGCGACACGCGCTACGTCGAAGATTTCACCAGCCGTCTGAACGGCATGGGCTCGGCTTCCAG
TCTGCAGAGCACCGTGGGCATCTACGGCACCGGCGAAACCTGGACCGCAGGCCTGATGGCCGACCGTTGGCAGCTCACCG
ACTACACGCTGGACGAGCAGGCCCTGCCCTATAACCGGCAGCCGCGCGCGTATTTCACCTGGGAAAAGCCGTTTGGCATT
TTCGAGGCGGGCGTCTACGCCGAAGCCGTGCGTTTCACCCATGACGACTCCTACTTCGTCCAGCCTCCGAGCCCCAGCGC
TCCAGGCGAAACAAACAACCGCGACGATAACGACGAGTACGTCCGTACCAATATCCGCAACAAGGAATATGGCAGCGGCG
CGCGCCTGGACGTCAAGCCGTATATCTCGATGCCGCTGTCGGGCGCAGCGTGGTTCTTGACGCCCACCGTGGCATGGCGC
TACACCGCGTACCAACTGGATTCCACGCTTGCCAACACCGCACCGCTGACCGGCAATCGCACCCCGTCGCGCAGCCTGCC
GATCGCCTCGCTGGACGCCGGCCTGTATTTCGATCGCGAAACCTCGCTGTTCGGCACCAACTATCTCAATACGTTGGAGC
CGCGCATGTACTACCTGTACGTGCCATACCGCGACCAGGACGATCTGCCGGTGTTCGACACCCGTCCGTTCACCTTCAGC
TACGGCCAGCTGTTCCGCGACACCCGCTACACCGGTGCCGACCGCCAGAACGATGCCAACCAGCTGACCCTGGCGGTGAC
CTCGCGTTGGCTGCGCCAGGACGATGGCCGCGAAAAGCTCTCGCTGAGCGCGGGCCAAATCCTGTACTTCAGCGACTCGC
TGGTGACCATCAACAACAGCAACAACTCGGCGGCCGGCAGCGAGCAGACCGTCGAGCAGGGCAAGTCGGCCTGGGTGGCC
GACGCCAACTACATGATCAACGACCGCTGGTCGATGGGCGCCACCTACCAGTGGAACCCGAACTCGCGCAAGGAAGATCT
GGCCAGCCTGCGCACGCGCTATCTGCTCAACAACGACGGCATCATCAACCTGGCGTATCGCTACCGTCGCAACCTCACCG
ACAACAGCGACCAGCTCAAACAGGCCGATTTCTCCTTCCTCTACCCGATCAACCCCAGCTGGAGCGCGGTCGGTCGGTAT
TACTACTCGCTTCTGGACCGCAAGCCGCTGGAAATCATCGGTGGCGTGCAATGGGACAGCTGCTGCCTGGCGGTACGCGG
ACTGGTGCGCCGATTCGTGCGCAACCGCGATGGCGAGATGGACAACTCGATCCAGATAGAGTTCGTGCTGAAGGGCTTGA
GCTCGTTCGGGCAGAACACGGACCGCACTTTGCGCCGTGCTATTCTCGGCTATTACCGCGACGACCTCTACCTGGTCCCG
CCCAGCAACACCACGACCAATCCGGACGATTACGATCCGAACCTGATTCCATGA

Upstream 100 bases:

>100_bases
CGGTCGCGCCACCGGTGGCGATGAACCTCGCCCACCCGGCGCCGGCGCTCTTCATTGCCCCTATGCGGGGTATGCGGCAA
GCTAGTCGCCATTTTATTGG

Downstream 100 bases:

>100_bases
CCAAGCCTTTCTCTGTTGTTCTCGCCTCGCTGCTGGCCATCACCAGCACGGTTTCGCCGTTGGCCTCGGCACAGCAATCC
CAGCCGCTGGATCGCATCGC

Product: organic solvent tolerance protein

Products: NA

Alternate protein names: Organic solvent tolerance protein [H]

Number of amino acids: Translated: 817; Mature: 817

Protein sequence:

>817_residues
MNPRVRRALRLLPLPLSIAICLPAMAADKPLNWGLCPAVDPLPGFDGAPAADPKAAEMRQQLPTDIEGDQLSGTSTTPQY
QGNVALKRGDQFLGADNLRMDTETGNYIAEGNVRYQDTSFRMVADRAEGNQDTDTHKVTNIQYQLVERRGNGDAESVDLQ
GQVGQMHRSTYTTCDPSQPIWRVRAPEIDVDNEEGFGTARNAVLQIGKVPVLYFPWFKFPIDDRRQTGLLFPQFGLSGRN
GFDYLQPIYLNLAPNYDATLLPRYMSKRGFMFGTEFRYLYEGGRGEVTGNYLPNDKLRDKDRGSVFYSGYHNVNTHWQAR
SSISWVSDTRYVEDFTSRLNGMGSASSLQSTVGIYGTGETWTAGLMADRWQLTDYTLDEQALPYNRQPRAYFTWEKPFGI
FEAGVYAEAVRFTHDDSYFVQPPSPSAPGETNNRDDNDEYVRTNIRNKEYGSGARLDVKPYISMPLSGAAWFLTPTVAWR
YTAYQLDSTLANTAPLTGNRTPSRSLPIASLDAGLYFDRETSLFGTNYLNTLEPRMYYLYVPYRDQDDLPVFDTRPFTFS
YGQLFRDTRYTGADRQNDANQLTLAVTSRWLRQDDGREKLSLSAGQILYFSDSLVTINNSNNSAAGSEQTVEQGKSAWVA
DANYMINDRWSMGATYQWNPNSRKEDLASLRTRYLLNNDGIINLAYRYRRNLTDNSDQLKQADFSFLYPINPSWSAVGRY
YYSLLDRKPLEIIGGVQWDSCCLAVRGLVRRFVRNRDGEMDNSIQIEFVLKGLSSFGQNTDRTLRRAILGYYRDDLYLVP
PSNTTTNPDDYDPNLIP

Sequences:

>Translated_817_residues
MNPRVRRALRLLPLPLSIAICLPAMAADKPLNWGLCPAVDPLPGFDGAPAADPKAAEMRQQLPTDIEGDQLSGTSTTPQY
QGNVALKRGDQFLGADNLRMDTETGNYIAEGNVRYQDTSFRMVADRAEGNQDTDTHKVTNIQYQLVERRGNGDAESVDLQ
GQVGQMHRSTYTTCDPSQPIWRVRAPEIDVDNEEGFGTARNAVLQIGKVPVLYFPWFKFPIDDRRQTGLLFPQFGLSGRN
GFDYLQPIYLNLAPNYDATLLPRYMSKRGFMFGTEFRYLYEGGRGEVTGNYLPNDKLRDKDRGSVFYSGYHNVNTHWQAR
SSISWVSDTRYVEDFTSRLNGMGSASSLQSTVGIYGTGETWTAGLMADRWQLTDYTLDEQALPYNRQPRAYFTWEKPFGI
FEAGVYAEAVRFTHDDSYFVQPPSPSAPGETNNRDDNDEYVRTNIRNKEYGSGARLDVKPYISMPLSGAAWFLTPTVAWR
YTAYQLDSTLANTAPLTGNRTPSRSLPIASLDAGLYFDRETSLFGTNYLNTLEPRMYYLYVPYRDQDDLPVFDTRPFTFS
YGQLFRDTRYTGADRQNDANQLTLAVTSRWLRQDDGREKLSLSAGQILYFSDSLVTINNSNNSAAGSEQTVEQGKSAWVA
DANYMINDRWSMGATYQWNPNSRKEDLASLRTRYLLNNDGIINLAYRYRRNLTDNSDQLKQADFSFLYPINPSWSAVGRY
YYSLLDRKPLEIIGGVQWDSCCLAVRGLVRRFVRNRDGEMDNSIQIEFVLKGLSSFGQNTDRTLRRAILGYYRDDLYLVP
PSNTTTNPDDYDPNLIP
>Mature_817_residues
MNPRVRRALRLLPLPLSIAICLPAMAADKPLNWGLCPAVDPLPGFDGAPAADPKAAEMRQQLPTDIEGDQLSGTSTTPQY
QGNVALKRGDQFLGADNLRMDTETGNYIAEGNVRYQDTSFRMVADRAEGNQDTDTHKVTNIQYQLVERRGNGDAESVDLQ
GQVGQMHRSTYTTCDPSQPIWRVRAPEIDVDNEEGFGTARNAVLQIGKVPVLYFPWFKFPIDDRRQTGLLFPQFGLSGRN
GFDYLQPIYLNLAPNYDATLLPRYMSKRGFMFGTEFRYLYEGGRGEVTGNYLPNDKLRDKDRGSVFYSGYHNVNTHWQAR
SSISWVSDTRYVEDFTSRLNGMGSASSLQSTVGIYGTGETWTAGLMADRWQLTDYTLDEQALPYNRQPRAYFTWEKPFGI
FEAGVYAEAVRFTHDDSYFVQPPSPSAPGETNNRDDNDEYVRTNIRNKEYGSGARLDVKPYISMPLSGAAWFLTPTVAWR
YTAYQLDSTLANTAPLTGNRTPSRSLPIASLDAGLYFDRETSLFGTNYLNTLEPRMYYLYVPYRDQDDLPVFDTRPFTFS
YGQLFRDTRYTGADRQNDANQLTLAVTSRWLRQDDGREKLSLSAGQILYFSDSLVTINNSNNSAAGSEQTVEQGKSAWVA
DANYMINDRWSMGATYQWNPNSRKEDLASLRTRYLLNNDGIINLAYRYRRNLTDNSDQLKQADFSFLYPINPSWSAVGRY
YYSLLDRKPLEIIGGVQWDSCCLAVRGLVRRFVRNRDGEMDNSIQIEFVLKGLSSFGQNTDRTLRRAILGYYRDDLYLVP
PSNTTTNPDDYDPNLIP

Specific function: Involved in the assembly of LPS in the outer leaflet of the outer membrane. Determines N-hexane tolerance and is involved in outer membrane permeability. Essential for envelope biogenesis [H]

COG id: COG1452

COG function: function code M; Organic solvent tolerance protein OstA

Gene ontology:

Cell location: Cell outer membrane [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lptD family [H]

Homologues:

Organism=Escherichia coli, GI1786239, Length=793, Percent_Identity=29.1298865069357, Blast_Score=281, Evalue=1e-76,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020889
- InterPro:   IPR005653
- InterPro:   IPR007543 [H]

Pfam domain/function: PF03968 OstA; PF04453 OstA_C [H]

EC number: NA

Molecular weight: Translated: 92710; Mature: 92710

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPRVRRALRLLPLPLSIAICLPAMAADKPLNWGLCPAVDPLPGFDGAPAADPKAAEMRQ
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
QLPTDIEGDQLSGTSTTPQYQGNVALKRGDQFLGADNLRMDTETGNYIAEGNVRYQDTSF
HCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEECCCCCEEECCCCCEEECCCEEEECCCE
RMVADRAEGNQDTDTHKVTNIQYQLVERRGNGDAESVDLQGQVGQMHRSTYTTCDPSQPI
EEEEECCCCCCCCCCEEEEEEEEEEEECCCCCCCCEEECCCHHHHHHHCCCCCCCCCCCE
WRVRAPEIDVDNEEGFGTARNAVLQIGKVPVLYFPWFKFPIDDRRQTGLLFPQFGLSGRN
EEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCEECCCCCCCCCCEEEECCCCCCCC
GFDYLQPIYLNLAPNYDATLLPRYMSKRGFMFGTEFRYLYEGGRGEVTGNYLPNDKLRDK
CCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEECCEEEEEECCCCCEECCCCCCCCCCCC
DRGSVFYSGYHNVNTHWQARSSISWVSDTRYVEDFTSRLNGMGSASSLQSTVGIYGTGET
CCCCEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCEEEEECCCC
WTAGLMADRWQLTDYTLDEQALPYNRQPRAYFTWEKPFGIFEAGVYAEAVRFTHDDSYFV
EEECCEECCEEECCEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHEEEECCCCEEE
QPPSPSAPGETNNRDDNDEYVRTNIRNKEYGSGARLDVKPYISMPLSGAAWFLTPTVAWR
CCCCCCCCCCCCCCCCCCHHHEECCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCEEEE
YTAYQLDSTLANTAPLTGNRTPSRSLPIASLDAGLYFDRETSLFGTNYLNTLEPRMYYLY
EEEEEHHHHHHCCCCCCCCCCCCCCCCEEEECCCEEECCCCCCCCCCCCCCCCCEEEEEE
VPYRDQDDLPVFDTRPFTFSYGQLFRDTRYTGADRQNDANQLTLAVTSRWLRQDDGREKL
EEECCCCCCCEECCCCCEEHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCEE
SLSAGQILYFSDSLVTINNSNNSAAGSEQTVEQGKSAWVADANYMINDRWSMGATYQWNP
EECCCEEEEEECCEEEEECCCCCCCCCHHHHHHCCCEEEECCCEEEECCCCCCCEEEECC
NSRKEDLASLRTRYLLNNDGIINLAYRYRRNLTDNSDQLKQADFSFLYPINPSWSAVGRY
CCCHHHHHHHHHHHEECCCCEEEEEEHHHCCCCCCHHHHHHCCCEEEEECCCCHHHHHHH
YYSLLDRKPLEIIGGVQWDSCCLAVRGLVRRFVRNRDGEMDNSIQIEFVLKGLSSFGQNT
HHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHCCCH
DRTLRRAILGYYRDDLYLVPPSNTTTNPDDYDPNLIP
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNPRVRRALRLLPLPLSIAICLPAMAADKPLNWGLCPAVDPLPGFDGAPAADPKAAEMRQ
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
QLPTDIEGDQLSGTSTTPQYQGNVALKRGDQFLGADNLRMDTETGNYIAEGNVRYQDTSF
HCCCCCCCCCCCCCCCCCCCCCCEEEECCCCEECCCCCEEECCCCCEEECCCEEEECCCE
RMVADRAEGNQDTDTHKVTNIQYQLVERRGNGDAESVDLQGQVGQMHRSTYTTCDPSQPI
EEEEECCCCCCCCCCEEEEEEEEEEEECCCCCCCCEEECCCHHHHHHHCCCCCCCCCCCE
WRVRAPEIDVDNEEGFGTARNAVLQIGKVPVLYFPWFKFPIDDRRQTGLLFPQFGLSGRN
EEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEECCEECCCCCCCCCCEEEECCCCCCCC
GFDYLQPIYLNLAPNYDATLLPRYMSKRGFMFGTEFRYLYEGGRGEVTGNYLPNDKLRDK
CCCCCCEEEEEECCCCCCCHHHHHHHCCCCEEECCEEEEEECCCCCEECCCCCCCCCCCC
DRGSVFYSGYHNVNTHWQARSSISWVSDTRYVEDFTSRLNGMGSASSLQSTVGIYGTGET
CCCCEEEECCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCEEEEECCCC
WTAGLMADRWQLTDYTLDEQALPYNRQPRAYFTWEKPFGIFEAGVYAEAVRFTHDDSYFV
EEECCEECCEEECCEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHEEEECCCCEEE
QPPSPSAPGETNNRDDNDEYVRTNIRNKEYGSGARLDVKPYISMPLSGAAWFLTPTVAWR
CCCCCCCCCCCCCCCCCCHHHEECCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCEEEE
YTAYQLDSTLANTAPLTGNRTPSRSLPIASLDAGLYFDRETSLFGTNYLNTLEPRMYYLY
EEEEEHHHHHHCCCCCCCCCCCCCCCCEEEECCCEEECCCCCCCCCCCCCCCCCEEEEEE
VPYRDQDDLPVFDTRPFTFSYGQLFRDTRYTGADRQNDANQLTLAVTSRWLRQDDGREKL
EEECCCCCCCEECCCCCEEHHHHHHHHCCCCCCCCCCCCCEEEEEEHHHHHHCCCCCCEE
SLSAGQILYFSDSLVTINNSNNSAAGSEQTVEQGKSAWVADANYMINDRWSMGATYQWNP
EECCCEEEEEECCEEEEECCCCCCCCCHHHHHHCCCEEEECCCEEEECCCCCCCEEEECC
NSRKEDLASLRTRYLLNNDGIINLAYRYRRNLTDNSDQLKQADFSFLYPINPSWSAVGRY
CCCHHHHHHHHHHHEECCCCEEEEEEHHHCCCCCCHHHHHHCCCEEEEECCCCHHHHHHH
YYSLLDRKPLEIIGGVQWDSCCLAVRGLVRRFVRNRDGEMDNSIQIEFVLKGLSSFGQNT
HHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHCCCH
DRTLRRAILGYYRDDLYLVPPSNTTTNPDDYDPNLIP
HHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]