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The map label for this gene is ntaA [H]

Identifier: 21241625

GI number: 21241625

Start: 1016136

End: 1017596

Strand: Direct

Name: ntaA [H]

Synonym: XAC0855

Alternate gene names: 21241625

Gene position: 1016136-1017596 (Clockwise)

Preceding gene: 21241624

Following gene: 21241626

Centisome position: 19.63

GC content: 66.6

Gene sequence:

>1461_bases
ATGAGCGGCAATGCCGACCCCAAACAATTGCACGTCAACCTGTTCGAAATGAACTGCGTCAGTCACATCGTGCACGGCAT
GTGGGCGCATCCGGACAACAACCGGCACCGGTTCAACGATCTGGAGTACTGGACCGAGCTGGCACAGCTGCTGGAATACG
GCACCTTCGACGGCATCTTTCTGGCCGATGTGATCGGCGCCTACGACAGCTTCGGGCACGGTGCGAAGGCCGCGCTGGAA
GAGGCGGTGCAGATTCCCAGCAACGATCCGCTGCTGTTGATCCCGGCGATGGCGCAGGTGACCAGGCATCTGGGCTTCGG
CGCGACCTTCTCCACCACCTACGAGCCGCCGTTCGCGTTCGCACGGCGCATGAGCACCCTCGATCACCTGACCCGCGGCC
GGGTCGGCTGGAACATCGTGACCTCCTACCTGCCCAATGCGGCACGCAACTTCGGCCTGGAAGGCGAGGTGCCGCACGAT
CAACGCTACGAGATCGCCGATGAATATCTGGACGTGCTGTACAAGCTGTGGGAAGGCTCGTGGGACGACGATGCGGTGAT
CCAGGACCGTGAGCGGCGCATCTACACCGATCCGTCCAAGGTGCGCCAGATCGATCACCACGGCGCCTACTTCAAGGTCG
CCGGCCCGCATCTGAGCCAGCCCTCGCCACAGCGCACGCCGCTGCTGTTCCAGGCTTCCGGCTCGGCGGCCGGGTTGGAA
TTCGCCGCGCGGCATGCCGAAGTGGTGTTCACCGGCGGGCTGACCGCCGCCGACGTGCGCGCCAATATCGCCAAGACCCG
CGCGCGCGTGGAAGCGCAGGGGCGCGATCCGCGCGCGATCCGGTTCGTGGTGCAGGCCGGGGTGATCGTCGGCCGGACCG
ATGCAGAGGTCGCGGCCAAACTCGACAGCTACCGCGCGCTGATGAGCGTGCGCGGCGGCCTGGTGCACGCGCAGTCGGAC
ATCGATCTGCTTGCCTATCCTCGCGATCTGGTAATCGGCGAGATCGTCGGCGGGCGCGGCAAGGGCAGCGCCAGCGGGAT
CGGCCGTGGCGTATTGGCCGGCTTCGACCCGCAGTGGACGGTCGGACAGGTGCTGACGCGGCTGGAGGCGTTCGACCGCG
AGCGGTATTTCGTTGCCGGCACGCCGACCGGCGTGGCCGACACCATCGAGCGCTGGCTCGACGAAGACGACATCGACGGC
ATCAACCTGCGCCAGTATCTGTCGTTCGAGACCGCACGCGATTTCATCGAACTGGTGGTGCCGGAACTGCGCCGGCGCGG
TCGCTTCCGCCAGACGTATCGCGATGGCGAGACGCTGCGCGAACGGCTGTTCGGCGCAGGCCAGGCACGCTTGCCGGACG
ATCATTTCGGTGCGCGTTATCGCGACCCCGCCAATCTCAAGGTGCCACCGCCGCCGCTGCGGTTTCCGGCGCCGTTGGCG
GCAGCCGGGAGCGGCGGATGA

Upstream 100 bases:

>100_bases
GGCGATTGAACGGTCGTCGTCCACGCGAGGCGTGGTTCGCCGGCACCACATCCCAGAGTGCGGCCGGCGCAGATGCGAGC
GTGTCGACGGGAGCGACTGC

Downstream 100 bases:

>100_bases
AGGCGGCGCTCGGCGTGGCGTTGGCGGTGCTGGCACTGGCCGGCTGTCGCCAGCAACCCGCGGCGTCGGCGGGTGCCGCC
GGCACCGCTCCGCGCCAGGG

Product: monooxygenase

Products: an aldehyde; SO3; H2O; FMN [C]

Alternate protein names: NTA monooxygenase component A; NTA-MO A [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSGNADPKQLHVNLFEMNCVSHIVHGMWAHPDNNRHRFNDLEYWTELAQLLEYGTFDGIFLADVIGAYDSFGHGAKAALE
EAVQIPSNDPLLLIPAMAQVTRHLGFGATFSTTYEPPFAFARRMSTLDHLTRGRVGWNIVTSYLPNAARNFGLEGEVPHD
QRYEIADEYLDVLYKLWEGSWDDDAVIQDRERRIYTDPSKVRQIDHHGAYFKVAGPHLSQPSPQRTPLLFQASGSAAGLE
FAARHAEVVFTGGLTAADVRANIAKTRARVEAQGRDPRAIRFVVQAGVIVGRTDAEVAAKLDSYRALMSVRGGLVHAQSD
IDLLAYPRDLVIGEIVGGRGKGSASGIGRGVLAGFDPQWTVGQVLTRLEAFDRERYFVAGTPTGVADTIERWLDEDDIDG
INLRQYLSFETARDFIELVVPELRRRGRFRQTYRDGETLRERLFGAGQARLPDDHFGARYRDPANLKVPPPPLRFPAPLA
AAGSGG

Sequences:

>Translated_486_residues
MSGNADPKQLHVNLFEMNCVSHIVHGMWAHPDNNRHRFNDLEYWTELAQLLEYGTFDGIFLADVIGAYDSFGHGAKAALE
EAVQIPSNDPLLLIPAMAQVTRHLGFGATFSTTYEPPFAFARRMSTLDHLTRGRVGWNIVTSYLPNAARNFGLEGEVPHD
QRYEIADEYLDVLYKLWEGSWDDDAVIQDRERRIYTDPSKVRQIDHHGAYFKVAGPHLSQPSPQRTPLLFQASGSAAGLE
FAARHAEVVFTGGLTAADVRANIAKTRARVEAQGRDPRAIRFVVQAGVIVGRTDAEVAAKLDSYRALMSVRGGLVHAQSD
IDLLAYPRDLVIGEIVGGRGKGSASGIGRGVLAGFDPQWTVGQVLTRLEAFDRERYFVAGTPTGVADTIERWLDEDDIDG
INLRQYLSFETARDFIELVVPELRRRGRFRQTYRDGETLRERLFGAGQARLPDDHFGARYRDPANLKVPPPPLRFPAPLA
AAGSGG
>Mature_485_residues
SGNADPKQLHVNLFEMNCVSHIVHGMWAHPDNNRHRFNDLEYWTELAQLLEYGTFDGIFLADVIGAYDSFGHGAKAALEE
AVQIPSNDPLLLIPAMAQVTRHLGFGATFSTTYEPPFAFARRMSTLDHLTRGRVGWNIVTSYLPNAARNFGLEGEVPHDQ
RYEIADEYLDVLYKLWEGSWDDDAVIQDRERRIYTDPSKVRQIDHHGAYFKVAGPHLSQPSPQRTPLLFQASGSAAGLEF
AARHAEVVFTGGLTAADVRANIAKTRARVEAQGRDPRAIRFVVQAGVIVGRTDAEVAAKLDSYRALMSVRGGLVHAQSDI
DLLAYPRDLVIGEIVGGRGKGSASGIGRGVLAGFDPQWTVGQVLTRLEAFDRERYFVAGTPTGVADTIERWLDEDDIDGI
NLRQYLSFETARDFIELVVPELRRRGRFRQTYRDGETLRERLFGAGQARLPDDHFGARYRDPANLKVPPPPLRFPAPLAA
AGSGG

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ntaA/snaA/soxA(dszA) monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1787166, Length=246, Percent_Identity=28.4552845528455, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1787247, Length=223, Percent_Identity=27.8026905829596, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011251
- InterPro:   IPR016048
- InterPro:   IPR016215 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: 1.14.14.5 [C]

Molecular weight: Translated: 53891; Mature: 53760

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGNADPKQLHVNLFEMNCVSHIVHGMWAHPDNNRHRFNDLEYWTELAQLLEYGTFDGIF
CCCCCCCHHEEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHH
LADVIGAYDSFGHGAKAALEEAVQIPSNDPLLLIPAMAQVTRHLGFGATFSTTYEPPFAF
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCEECCCCCCHHHH
ARRMSTLDHLTRGRVGWNIVTSYLPNAARNFGLEGEVPHDQRYEIADEYLDVLYKLWEGS
HHHHHHHHHHHCCCCCHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
WDDDAVIQDRERRIYTDPSKVRQIDHHGAYFKVAGPHLSQPSPQRTPLLFQASGSAAGLE
CCCCHHHHCCCCCEECCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCCHH
FAARHAEVVFTGGLTAADVRANIAKTRARVEAQGRDPRAIRFVVQAGVIVGRTDAEVAAK
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCEEEECCCHHHHHH
LDSYRALMSVRGGLVHAQSDIDLLAYPRDLVIGEIVGGRGKGSASGIGRGVLAGFDPQWT
HHHHHHHHHHCCCEEEECCCCEEEECCHHHHEEHHCCCCCCCCCCCCCCCCCCCCCCCCC
VGQVLTRLEAFDRERYFVAGTPTGVADTIERWLDEDDIDGINLRQYLSFETARDFIELVV
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
PELRRRGRFRQTYRDGETLRERLFGAGQARLPDDHFGARYRDPANLKVPPPPLRFPAPLA
HHHHHCCHHHHHHCCHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCC
AAGSGG
CCCCCC
>Mature Secondary Structure 
SGNADPKQLHVNLFEMNCVSHIVHGMWAHPDNNRHRFNDLEYWTELAQLLEYGTFDGIF
CCCCCCHHEEEEHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHH
LADVIGAYDSFGHGAKAALEEAVQIPSNDPLLLIPAMAQVTRHLGFGATFSTTYEPPFAF
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCEECCCCCCHHHH
ARRMSTLDHLTRGRVGWNIVTSYLPNAARNFGLEGEVPHDQRYEIADEYLDVLYKLWEGS
HHHHHHHHHHHCCCCCHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
WDDDAVIQDRERRIYTDPSKVRQIDHHGAYFKVAGPHLSQPSPQRTPLLFQASGSAAGLE
CCCCHHHHCCCCCEECCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCCHH
FAARHAEVVFTGGLTAADVRANIAKTRARVEAQGRDPRAIRFVVQAGVIVGRTDAEVAAK
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCEEEECCCHHHHHH
LDSYRALMSVRGGLVHAQSDIDLLAYPRDLVIGEIVGGRGKGSASGIGRGVLAGFDPQWT
HHHHHHHHHHCCCEEEECCCCEEEECCHHHHEEHHCCCCCCCCCCCCCCCCCCCCCCCCC
VGQVLTRLEAFDRERYFVAGTPTGVADTIERWLDEDDIDGINLRQYLSFETARDFIELVV
HHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
PELRRRGRFRQTYRDGETLRERLFGAGQARLPDDHFGARYRDPANLKVPPPPLRFPAPLA
HHHHHCCHHHHHHCCHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCC
AAGSGG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: an alkanesulfonate; O2; FMNH2 [C]

Specific reaction: an alkanesulfonate + O2 + FMNH2 = an aldehyde + SO3 + H2O + FMN [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]