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The map label for this gene is gfa

Identifier: 21241506

GI number: 21241506

Start: 875317

End: 875892

Strand: Direct

Name: gfa

Synonym: XAC0735

Alternate gene names: 21241506

Gene position: 875317-875892 (Clockwise)

Preceding gene: 21241505

Following gene: 21241507

Centisome position: 16.91

GC content: 60.59

Gene sequence:

>576_bases
ATGACTAGCGTGTCGATTCATCCTTCGGTAGATGGTGGTGTTGCGCGAGGCGGCGCAGAAGGTTTTCAAGGCGGCACGCT
TGAATGCCACTGCGCCAGCGACAAGGTGACCGTCGAGGTGTCTGCGCAGACCGCGCATAACCATGCTTGCGGTTGCAGTA
AATGCTGGAAACCCGATGGCGCGAAGTTCTCGGTCGTGGCGGTGGTTCCGCGCGACAACGTCGAGGTCACCGCCCACCAG
GAAAAGTTGAAGATCGTCGACGAAAGCGCGACGATCCAGCGCCATGCGTGCACCGGTTGCGGCGTACACCTGTACGGGCG
GATCGAAAACAAGGACCATGCGTTCTACGGTCTGGATTTTGTGCACACCGAGTTGTCGAAACAACAGGGCTGGTCGGCAC
CGGGCTTTGCCGCGTTCGTCTCGTCGATCATCGAAACCGGCACGCCGCCGGAGCAGATGGATGAGGTACGCGCGCGTCTG
ACCGAACTGGGGTTGCCGCCCTACGACTGCCTCTCCCCAGCGTTGATGGATGCCCTGTCGGCCCATGTGGCACGCAAGAA
GGGTCTGTTGCATTGA

Upstream 100 bases:

>100_bases
GCAAGTCGATCCGCACCGTGATCCATTTCTGACCAGGCGGCCAGGAGCGGCGCGCGGCCGCTTCCGCGCACGTAGTACCC
GACACTCCGGGAGGGGCATG

Downstream 100 bases:

>100_bases
TGGTCCAACCCTGCAACCGGCGATCCGGCTGCAGGGTTTTCTCTTCGTAAGGAATCTCATGGAACGTATCGAACACCGCG
CCTGCTTCGGCGGCTGGCAG

Product: glutathione-dependent formaldehyde-activating enzyme

Products: NA

Alternate protein names: S-(hydroxymethyl)glutathione synthase

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MTSVSIHPSVDGGVARGGAEGFQGGTLECHCASDKVTVEVSAQTAHNHACGCSKCWKPDGAKFSVVAVVPRDNVEVTAHQ
EKLKIVDESATIQRHACTGCGVHLYGRIENKDHAFYGLDFVHTELSKQQGWSAPGFAAFVSSIIETGTPPEQMDEVRARL
TELGLPPYDCLSPALMDALSAHVARKKGLLH

Sequences:

>Translated_191_residues
MTSVSIHPSVDGGVARGGAEGFQGGTLECHCASDKVTVEVSAQTAHNHACGCSKCWKPDGAKFSVVAVVPRDNVEVTAHQ
EKLKIVDESATIQRHACTGCGVHLYGRIENKDHAFYGLDFVHTELSKQQGWSAPGFAAFVSSIIETGTPPEQMDEVRARL
TELGLPPYDCLSPALMDALSAHVARKKGLLH
>Mature_190_residues
TSVSIHPSVDGGVARGGAEGFQGGTLECHCASDKVTVEVSAQTAHNHACGCSKCWKPDGAKFSVVAVVPRDNVEVTAHQE
KLKIVDESATIQRHACTGCGVHLYGRIENKDHAFYGLDFVHTELSKQQGWSAPGFAAFVSSIIETGTPPEQMDEVRARLT
ELGLPPYDCLSPALMDALSAHVARKKGLLH

Specific function: Catalyzes the condensation of formaldehyde and glutathione to S-hydroxymethylglutathione

COG id: COG3791

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gfa family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GFA_XANAC (Q8PPF1)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641088.1
- ProteinModelPortal:   Q8PPF1
- SMR:   Q8PPF1
- GeneID:   1154806
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0735
- NMPDR:   fig|190486.1.peg.732
- HOGENOM:   HBG294467
- OMA:   AQTAHNH
- ProtClustDB:   PRK05417
- BioCyc:   XAXO190486:XAC0735-MONOMER
- BRENDA:   4.4.1.22
- HAMAP:   MF_00723
- InterPro:   IPR014185
- InterPro:   IPR006913
- InterPro:   IPR011057
- PIRSF:   PIRSF033318
- TIGRFAMs:   TIGR02820

Pfam domain/function: PF04828 GFA; SSF51316 Mss4_like

EC number: =4.4.1.22

Molecular weight: Translated: 20393; Mature: 20262

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVSIHPSVDGGVARGGAEGFQGGTLECHCASDKVTVEVSAQTAHNHACGCSKCWKPDG
CCCEECCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCC
AKFSVVAVVPRDNVEVTAHQEKLKIVDESATIQRHACTGCGVHLYGRIENKDHAFYGLDF
CCEEEEEEECCCCEEEEEECCEEEEECCCCHHHHHCCCCCCEEEEEEECCCCCEEEEHHH
VHTELSKQQGWSAPGFAAFVSSIIETGTPPEQMDEVRARLTELGLPPYDCLSPALMDALS
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AHVARKKGLLH
HHHHHHCCCCC
>Mature Secondary Structure 
TSVSIHPSVDGGVARGGAEGFQGGTLECHCASDKVTVEVSAQTAHNHACGCSKCWKPDG
CCEECCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCCCCCCCCCCC
AKFSVVAVVPRDNVEVTAHQEKLKIVDESATIQRHACTGCGVHLYGRIENKDHAFYGLDF
CCEEEEEEECCCCEEEEEECCEEEEECCCCHHHHHCCCCCCEEEEEEECCCCCEEEEHHH
VHTELSKQQGWSAPGFAAFVSSIIETGTPPEQMDEVRARLTELGLPPYDCLSPALMDALS
HHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AHVARKKGLLH
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217