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The map label for this gene is gsh1

Identifier: 21241499

GI number: 21241499

Start: 867580

End: 868944

Strand: Direct

Name: gsh1

Synonym: XAC0728

Alternate gene names: NA

Gene position: 867580-868944 (Clockwise)

Preceding gene: 21241496

Following gene: 21241502

Centisome position: 16.76

GC content: 64.69

Gene sequence:

>1365_bases
TTGTCGAGTCCTAGCCACGTTGCCGAAACGCCGATCACCGAACGTGCGGAGCTGGCCCAGGTGCTGGCTTCCGGCGAGAA
GCCGAGTGCGGACTGGCGGATCGGCACCGAGCACGAGAAGTTCGGCTTCCAGTTGGATGACCTGCGTGCGCCCACTTTCG
ACGGTGCACGCGGTATCGAGGCCTTGCTGACCGGTCTGACCCGTTTCGGCTGGGAGCCGGTCCAGGAAAACGGCCGCACC
ATCGCGCTGCTGCGCGACGGCGCATCGGTCACGCTGGAGCCGGCCGGACAGCTGGAGCTCTCCGGCGCGGCGGTGGAAAC
CCTGCACCATACGTGCGTGGAAACCGGTACCCATCTGGACGAAGTGGCGCAGGTCGCCGGCGAGTTGCAGCTGGGCTTTC
TGGGCATGGGCTTCCAGCCCAAGTGGCGTCGCGACCAGATGCCGTGGATGCCCAAGGGCCGCTACCAGATCATGAAGAAC
TACATGCCCAAGGTCGGCTCGCTCGGCCTGGACATGATGACGCGCACCTGCACGGTGCAGGTCAACCTGGATTACGCCAC
CGAAGCGGACATGGTGAAGAAGTTCCGAGTGTCGCTGGCGTTGCAGCCAATCGCCACCGCGTTGTTCGCCGATTCGCCGT
TCACCGACGGCAAGCCGAACGGCTATCTCAGCTACCGCTCGCACATCTGGACCGACACCGACGGCGATCGCACCGGCATG
CTGGATTTCGTGTTCGAAGACGGCTTCGGCTACGACCGCTACGTCGATTACCTGCTCGATGTGCCGATGTATTTTTCCTA
CCGCGATGGCGTGTATCACGACGCCAGCGGGCAGAGCTTCCGCGATTTCATGCAGGGCAAGCTGCCTGCGCTGCCGGGCG
CGTTGCCGACATTGCGCGATTGGTCCGACCACATGACCACCGCATTTCCGGAAGTGCGTTTGAAGAAGTATCTGGAAATG
CGCGGTGCCGATGCCGGCCCCTGGGACCGGTTGTGCGCGTTGTCGGCGTTCTGGGTCGGCCTGCTGTACGACCAGACCGC
GCTGGATGCGGCCTGGGACCTGGTCAAGGATTTCAGCCCGACCGAGCGGCACGCGCTGCGCGACGGCGTGCCCAAGCATG
CGCTGGGCCTGCCGTTCCGCAATGGCACCGCGCGCGATCTGGCAGTGGAGGCGGTCAACATCGCGCGCGAAGGCCTGCGT
CGCCGCGCACGCCTGAATCGCGACGGGCAGGACGAAACCGGCTTTCTGGATGTGATCGCCGAGATCGCCGAAAGCGGCGT
CACCGCTGCCGAACGCAAGCTGGCGCTGTATCACGGCGCATGGAACGGCGATATCGACCGCGTGTTCCGCGAGTTCGCTT
ACTGA

Upstream 100 bases:

>100_bases
GGCGGCAATTCTTTCCATGCCCTGCGGTTATAGTGCGGGCCTTGCGGTGGCAGGGGAGTATCCCCACATCGTCCCACCCC
TTTCGTTTGCGAGCCTGCCC

Downstream 100 bases:

>100_bases
GCGCGCCTTGGCCGGTTGCCAGCGCGTGTTGGCGCTGGCGATCCGGTGGGCGTCGGTGATGGCTGGCGGCGCCGGTGTCG
CCGCGTCAGCCGATGTAGCC

Product: glutamate-cysteine ligase

Products: ADP; phosphate; gamma-L-glutamyl-L-cysteine

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein; Glutamate-Cysteine Ligase GSHA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSSPSHVAETPITERAELAQVLASGEKPSADWRIGTEHEKFGFQLDDLRAPTFDGARGIEALLTGLTRFGWEPVQENGRT
IALLRDGASVTLEPAGQLELSGAAVETLHHTCVETGTHLDEVAQVAGELQLGFLGMGFQPKWRRDQMPWMPKGRYQIMKN
YMPKVGSLGLDMMTRTCTVQVNLDYATEADMVKKFRVSLALQPIATALFADSPFTDGKPNGYLSYRSHIWTDTDGDRTGM
LDFVFEDGFGYDRYVDYLLDVPMYFSYRDGVYHDASGQSFRDFMQGKLPALPGALPTLRDWSDHMTTAFPEVRLKKYLEM
RGADAGPWDRLCALSAFWVGLLYDQTALDAAWDLVKDFSPTERHALRDGVPKHALGLPFRNGTARDLAVEAVNIAREGLR
RRARLNRDGQDETGFLDVIAEIAESGVTAAERKLALYHGAWNGDIDRVFREFAY

Sequences:

>Translated_454_residues
MSSPSHVAETPITERAELAQVLASGEKPSADWRIGTEHEKFGFQLDDLRAPTFDGARGIEALLTGLTRFGWEPVQENGRT
IALLRDGASVTLEPAGQLELSGAAVETLHHTCVETGTHLDEVAQVAGELQLGFLGMGFQPKWRRDQMPWMPKGRYQIMKN
YMPKVGSLGLDMMTRTCTVQVNLDYATEADMVKKFRVSLALQPIATALFADSPFTDGKPNGYLSYRSHIWTDTDGDRTGM
LDFVFEDGFGYDRYVDYLLDVPMYFSYRDGVYHDASGQSFRDFMQGKLPALPGALPTLRDWSDHMTTAFPEVRLKKYLEM
RGADAGPWDRLCALSAFWVGLLYDQTALDAAWDLVKDFSPTERHALRDGVPKHALGLPFRNGTARDLAVEAVNIAREGLR
RRARLNRDGQDETGFLDVIAEIAESGVTAAERKLALYHGAWNGDIDRVFREFAY
>Mature_453_residues
SSPSHVAETPITERAELAQVLASGEKPSADWRIGTEHEKFGFQLDDLRAPTFDGARGIEALLTGLTRFGWEPVQENGRTI
ALLRDGASVTLEPAGQLELSGAAVETLHHTCVETGTHLDEVAQVAGELQLGFLGMGFQPKWRRDQMPWMPKGRYQIMKNY
MPKVGSLGLDMMTRTCTVQVNLDYATEADMVKKFRVSLALQPIATALFADSPFTDGKPNGYLSYRSHIWTDTDGDRTGML
DFVFEDGFGYDRYVDYLLDVPMYFSYRDGVYHDASGQSFRDFMQGKLPALPGALPTLRDWSDHMTTAFPEVRLKKYLEMR
GADAGPWDRLCALSAFWVGLLYDQTALDAAWDLVKDFSPTERHALRDGVPKHALGLPFRNGTARDLAVEAVNIAREGLRR
RARLNRDGQDETGFLDVIAEIAESGVTAAERKLALYHGAWNGDIDRVFREFAY

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.2

Molecular weight: Translated: 50734; Mature: 50603

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPSHVAETPITERAELAQVLASGEKPSADWRIGTEHEKFGFQLDDLRAPTFDGARGIE
CCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCCHHHHCCCHHHCCCCCCCCHHHHH
ALLTGLTRFGWEPVQENGRTIALLRDGASVTLEPAGQLELSGAAVETLHHTCVETGTHLD
HHHHHHHHHCCCHHHCCCCEEEEEECCCEEEECCCCCEEECCHHHHHHHHHHHHCCCCHH
EVAQVAGELQLGFLGMGFQPKWRRDQMPWMPKGRYQIMKNYMPKVGSLGLDMMTRTCTVQ
HHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHEEEEEEE
VNLDYATEADMVKKFRVSLALQPIATALFADSPFTDGKPNGYLSYRSHIWTDTDGDRTGM
EEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCEECCCCCCCCCCC
LDFVFEDGFGYDRYVDYLLDVPMYFSYRDGVYHDASGQSFRDFMQGKLPALPGALPTLRD
EEEEEECCCCHHHHHHHHHCCCCEEEECCCCEECCCCHHHHHHHCCCCCCCCCCCCCHHH
WSDHMTTAFPEVRLKKYLEMRGADAGPWDRLCALSAFWVGLLYDQTALDAAWDLVKDFSP
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TERHALRDGVPKHALGLPFRNGTARDLAVEAVNIAREGLRRRARLNRDGQDETGFLDVIA
HHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
EIAESGVTAAERKLALYHGAWNGDIDRVFREFAY
HHHHCCCHHHHHEEEEEECCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SSPSHVAETPITERAELAQVLASGEKPSADWRIGTEHEKFGFQLDDLRAPTFDGARGIE
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEECCCHHHHCCCHHHCCCCCCCCHHHHH
ALLTGLTRFGWEPVQENGRTIALLRDGASVTLEPAGQLELSGAAVETLHHTCVETGTHLD
HHHHHHHHHCCCHHHCCCCEEEEEECCCEEEECCCCCEEECCHHHHHHHHHHHHCCCCHH
EVAQVAGELQLGFLGMGFQPKWRRDQMPWMPKGRYQIMKNYMPKVGSLGLDMMTRTCTVQ
HHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHEEEEEEE
VNLDYATEADMVKKFRVSLALQPIATALFADSPFTDGKPNGYLSYRSHIWTDTDGDRTGM
EEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCEECCCCCCCCCCC
LDFVFEDGFGYDRYVDYLLDVPMYFSYRDGVYHDASGQSFRDFMQGKLPALPGALPTLRD
EEEEEECCCCHHHHHHHHHCCCCEEEECCCCEECCCCHHHHHHHCCCCCCCCCCCCCHHH
WSDHMTTAFPEVRLKKYLEMRGADAGPWDRLCALSAFWVGLLYDQTALDAAWDLVKDFSP
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TERHALRDGVPKHALGLPFRNGTARDLAVEAVNIAREGLRRRARLNRDGQDETGFLDVIA
HHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
EIAESGVTAAERKLALYHGAWNGDIDRVFREFAY
HHHHCCCHHHHHEEEEEECCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; L-cysteine

Specific reaction: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA