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The map label for this gene is cca

Identifier: 21241488

GI number: 21241488

Start: 854150

End: 855382

Strand: Reverse

Name: cca

Synonym: XAC0717

Alternate gene names: 21241488

Gene position: 855382-854150 (Counterclockwise)

Preceding gene: 21241489

Following gene: 77748547

Centisome position: 16.53

GC content: 66.34

Gene sequence:

>1233_bases
ATGAAGATCTATCTTGTTGGCGGCGCAGTCCGCGATGCATTGCTTGAGCAGCCGGCTGGCGACCGCGACTGGGTGGTGGT
CGGCGCCGATCAGGCGCAGATGGAGGCGCAGGGGTTCAAGCCGGTCGGCAAGGATTTCCCGGTGTTTCTGCACCCGCGCA
GTGGCGAGGAATACGCACTGGCGCGTACCGAGCGCAAATCCGGCCGCGGATATCGCGGCTTCGTGGTGGACGCCGACCCA
TCGGTGACCCTGGAAGAAGACCTGCTGCGCCGCGACTTCACCATCAACGCGATCGCGCGCGATGAGGACACGGGCCAATT
GTTCGACCCGTACAACGGTGTGCGTGATCTGCAGGCGCGCGTATTGCGGCATGTCGGTCCGGCGTTCATCGAAGATCCTG
TGCGTGTATTGCGTGCCGCGCGCTTCATGGCGCGGCTGGCCCCGCTGGGCTTTTCGCTCGCTGCTGAAACTGCGGCATTG
ATGCGGGACATGGCCGCCGGCGGCGAACTCGACAGCCTGGTGCCGGAGCGCGTGTGGCAGGAGCTGCGGCGCGCATTGAG
TTGCGCGCAGCCCTCGGCGTTCCTGCGCACGCTGCACGATGCCGATGCGCTGCGCGTCATCCTGCCGGAAGTCGATGCGC
TGTACGGCGTGCCGCAGCGCGCCGATTTCCATCCGGAGGTCGACACGGGCATTCACCAGGAAATGGTCAGCGATATAGCG
GCACGTCTGGCGCCCGGCGATGCGCTGGTGGGCTTTGCTGCACTGACCCACGACCTGGGTAAGGCGCTCACGCCGCAAGC
GCAATGGCCGCGACATGTCATGCACGAGCAACGCGGCGTTGCGCCATTGCAGGCGCTGTGCGAGCGGCTCAAGGTGCCGC
AGGACTTTCGGCAACTTGCCATCATCGCCTGCCGCGAACACTTGAACGTGCATCGCCTGGCCGAGCTCCGCGACCGCACC
CTGCACGAGCTGCTGGTGCGCTGCGATGCGTTTCGCCGGCCGGAGCGTATCGCGCAGCTGGCACTGGTCTGCGAAGCAGA
CAAGCGTGGCCGTCTGGGCAGTGAAGAAGCCGCGTACCCGCAGGGCGAAGCGCTCAAACGGCTGCACGCCGCCGCACTGG
CGATCAATGCGCGGGATCTGGCGGCCGAGGGTCTGCAAGGGCCGCAGATCGGCGAGGCACTGACGAAGGCGCGCATTGCC
GCGATTGCGGCAGCGCGAAACACTGGTGCATAG

Upstream 100 bases:

>100_bases
CGTCCATGAGCGCGTTAGCGGCGTCCACGCCGCCAATGGTCCCGCAAGCAGCCGGGAGAGCGCACAGACAGGCGCATGAT
TGCTCGGGCATCATGCGCTG

Downstream 100 bases:

>100_bases
CGACGTCTCGCGCGCACCGTTTTCTGCATGCAACAGGATGCGGCAACGTTAGAAAGAGCGACATTGCAGCGAACCGCACG
GCTGACGGAATCTGCCCTTC

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MKIYLVGGAVRDALLEQPAGDRDWVVVGADQAQMEAQGFKPVGKDFPVFLHPRSGEEYALARTERKSGRGYRGFVVDADP
SVTLEEDLLRRDFTINAIARDEDTGQLFDPYNGVRDLQARVLRHVGPAFIEDPVRVLRAARFMARLAPLGFSLAAETAAL
MRDMAAGGELDSLVPERVWQELRRALSCAQPSAFLRTLHDADALRVILPEVDALYGVPQRADFHPEVDTGIHQEMVSDIA
ARLAPGDALVGFAALTHDLGKALTPQAQWPRHVMHEQRGVAPLQALCERLKVPQDFRQLAIIACREHLNVHRLAELRDRT
LHELLVRCDAFRRPERIAQLALVCEADKRGRLGSEEAAYPQGEALKRLHAAALAINARDLAAEGLQGPQIGEALTKARIA
AIAAARNTGA

Sequences:

>Translated_410_residues
MKIYLVGGAVRDALLEQPAGDRDWVVVGADQAQMEAQGFKPVGKDFPVFLHPRSGEEYALARTERKSGRGYRGFVVDADP
SVTLEEDLLRRDFTINAIARDEDTGQLFDPYNGVRDLQARVLRHVGPAFIEDPVRVLRAARFMARLAPLGFSLAAETAAL
MRDMAAGGELDSLVPERVWQELRRALSCAQPSAFLRTLHDADALRVILPEVDALYGVPQRADFHPEVDTGIHQEMVSDIA
ARLAPGDALVGFAALTHDLGKALTPQAQWPRHVMHEQRGVAPLQALCERLKVPQDFRQLAIIACREHLNVHRLAELRDRT
LHELLVRCDAFRRPERIAQLALVCEADKRGRLGSEEAAYPQGEALKRLHAAALAINARDLAAEGLQGPQIGEALTKARIA
AIAAARNTGA
>Mature_410_residues
MKIYLVGGAVRDALLEQPAGDRDWVVVGADQAQMEAQGFKPVGKDFPVFLHPRSGEEYALARTERKSGRGYRGFVVDADP
SVTLEEDLLRRDFTINAIARDEDTGQLFDPYNGVRDLQARVLRHVGPAFIEDPVRVLRAARFMARLAPLGFSLAAETAAL
MRDMAAGGELDSLVPERVWQELRRALSCAQPSAFLRTLHDADALRVILPEVDALYGVPQRADFHPEVDTGIHQEMVSDIA
ARLAPGDALVGFAALTHDLGKALTPQAQWPRHVMHEQRGVAPLQALCERLKVPQDFRQLAIIACREHLNVHRLAELRDRT
LHELLVRCDAFRRPERIAQLALVCEADKRGRLGSEEAAYPQGEALKRLHAAALAINARDLAAEGLQGPQIGEALTKARIA
AIAAARNTGA

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789436, Length=407, Percent_Identity=54.5454545454545, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI87081691, Length=255, Percent_Identity=28.2352941176471, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_XANAC (Q8PPG9)

Other databases:

- EMBL:   AE008923
- RefSeq:   NP_641070.1
- ProteinModelPortal:   Q8PPG9
- SMR:   Q8PPG9
- GeneID:   1154788
- GenomeReviews:   AE008923_GR
- KEGG:   xac:XAC0717
- NMPDR:   fig|190486.1.peg.714
- HOGENOM:   HBG635063
- OMA:   GHGQKGL
- ProtClustDB:   PRK10885
- BioCyc:   XAXO190486:XAC0717-MONOMER
- BRENDA:   2.7.7.21
- BRENDA:   2.7.7.25
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 45064; Mature: 45064

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 138-138 BINDING 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYLVGGAVRDALLEQPAGDRDWVVVGADQAQMEAQGFKPVGKDFPVFLHPRSGEEYAL
CEEEEECHHHHHHHHHCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEEECCCCCCHHH
ARTERKSGRGYRGFVVDADPSVTLEEDLLRRDFTINAIARDEDTGQLFDPYNGVRDLQAR
HHHHHHCCCCCEEEEEECCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
VLRHVGPAFIEDPVRVLRAARFMARLAPLGFSLAAETAALMRDMAAGGELDSLVPERVWQ
HHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ELRRALSCAQPSAFLRTLHDADALRVILPEVDALYGVPQRADFHPEVDTGIHQEMVSDIA
HHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
ARLAPGDALVGFAALTHDLGKALTPQAQWPRHVMHEQRGVAPLQALCERLKVPQDFRQLA
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCHHHHHHH
IIACREHLNVHRLAELRDRTLHELLVRCDAFRRPERIAQLALVCEADKRGRLGSEEAAYP
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
QGEALKRLHAAALAINARDLAAEGLQGPQIGEALTKARIAAIAAARNTGA
CHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKIYLVGGAVRDALLEQPAGDRDWVVVGADQAQMEAQGFKPVGKDFPVFLHPRSGEEYAL
CEEEEECHHHHHHHHHCCCCCCCEEEECCCHHHHHHCCCCCCCCCCCEEEECCCCCCHHH
ARTERKSGRGYRGFVVDADPSVTLEEDLLRRDFTINAIARDEDTGQLFDPYNGVRDLQAR
HHHHHHCCCCCEEEEEECCCCCCHHHHHHHCCCEEEEEECCCCCCCCCCCCCHHHHHHHH
VLRHVGPAFIEDPVRVLRAARFMARLAPLGFSLAAETAALMRDMAAGGELDSLVPERVWQ
HHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ELRRALSCAQPSAFLRTLHDADALRVILPEVDALYGVPQRADFHPEVDTGIHQEMVSDIA
HHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHH
ARLAPGDALVGFAALTHDLGKALTPQAQWPRHVMHEQRGVAPLQALCERLKVPQDFRQLA
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCCHHHHHHH
IIACREHLNVHRLAELRDRTLHELLVRCDAFRRPERIAQLALVCEADKRGRLGSEEAAYP
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
QGEALKRLHAAALAINARDLAAEGLQGPQIGEALTKARIAAIAAARNTGA
CHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217