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The map label for this gene is lacZ [H]

Identifier: 21241479

GI number: 21241479

Start: 839320

End: 841101

Strand: Direct

Name: lacZ [H]

Synonym: XAC0708

Alternate gene names: 21241479

Gene position: 839320-841101 (Clockwise)

Preceding gene: 21241478

Following gene: 21241480

Centisome position: 16.22

GC content: 65.77

Gene sequence:

>1782_bases
GTGGCGTTGCCCGATGCGCTGCATGTGTTTCGTCTGCAGCGGCTGAAATCGATTGGCTGCAACGCCATCCGTCTGCATCA
CGCAGTGGCGCCGGAACTGCTGCAGGCCTGCGACCGTCTCGGCATGTTGGTGATGGCCGAGAACCGCTTGTTCAATCCTG
CACCCGACTATGTGGCGTTGCTGCGCAACATGGTGCGGCGCCAGCGCAATCATCCCAGCGTGTTTCTGTGGTCGCTGCTC
AACGAAGAGCCGCTGCAAGGCACCGCCACCGGTTACGCGATCACGGCGCGCGCCGCCAGCGCGGTGCGGGCGCTCGACGA
CACGCGCCCAATCACCGCCGGCATGAACGATGGCATGTTCGCCACCGGCGGCGCGGCCGATGTGGTGGACGTGCTCGGTT
TCAACTATCGGCAGTACAACTACGACCGCGTGCATGCAGCGCGTCCGCGCACGCCGATGCTGTCCAGCGAAGACACCAGT
GCGTTTCAGACGCGTGGTGCGTGGTTCACCGATCTGGATGCGCACGTGGTGGCCGAAGACGACAGCCTGGCCGCGGACTG
GGGCAATACCCATCGCCGCGCCTGGCAGCTGATTGCCGAACGCCCGTTCGTCGCCGGCAGTTTCGTGTGGACCGGCTTCG
ATTATCGCGGGGAACCCACGCCGTTCGAATGGCCATCGGTGGGGGCGTTCTTCGGCATCATGGACCAATGCGGATTTGCC
AAGGGCGCCTATTGGCTGCGTCGCGCCTTATGGATCGACGATGCGCCGGTACTGCATCTGTTGCCACACTGGAACTGGCC
CGGCCGCGAAGGTCAGCCGATCAAGGTCATGGCGTTCTGCAATGCCGAGCAGGTGGAGTTGTGGCTCAACGGCCGCTCGC
TCGGGAAACAAGCGGTGGATCGTGCGCAATCGAATCAGTGGCAAGTGGACTACGCGCCGGGCGTGCTCGAAGCGGTGGCC
TGGCGTGGGGGACACGAAGTGGCGCGCACGCGCGTCGAAACCACCGGCGCTCCGGTCGCGCTCAAACTCACCCCCGACCG
CAGCGAACTGCACGGCGATGGCCGCGATGCGCAACCGGTGACGTTGGAAGCGGTGGACGCGCAGGGGCGCCACGTGCCCG
ATTGCAATGCACTGCTGACGCTGCAGATCAGCGGCGGCCGGCTGTTGGGTATCGGCAATGGCGACCCGAATTCGCATGCA
GCGGATCAAAGCACGCAGGTGGCGTTGTTCAACGGGCTGGCGCAGGCAATCGTGCAAGCCGGGAGCGGTGCGGGCGTGTT
GCGCCTGCAGGCAAGTAGCCCCGGATTGCGCGATGCAGTGCTTGATATCGGCTGCACGTCGGCGCCGCTGCCGGCATCGG
TGCCGATCGCGGCGCCAGTGATGGTGGTCGAAAGCTGGCGCCACACCGCTGCATTCGCGCAGCCGCCCGCACCGGACCTC
CCGCGCCGTCCTAACGACAACAACAGCTGGAGCAATACGTTGCCAGGCACCTTGGAAAGCGCCCCCGAACGTGCCGGCTA
TGTGTTGTATCGCAGTCAATTCACACCGTGGCAGGGCATGCAAACCCGCGGCGGCATACTCGACCTGGGCCGCCTCAGCG
GGCCGGCGCAGGTATTTCTGGACGGCCGCGCGGTCGCCCAGGCAGCGGCCGGAACACCGGTCACCGTGGCGCTTGCACCC
GCGGCCGGCGAGCGCACCCTGGCGGTGATTCTGCAGGTCGCTGCCGATCAGCCATTTGGATTCCACGACGTCGTCACCGT
GCATTATCGGAACCCGCCATGA

Upstream 100 bases:

>100_bases
TCGTAGCCTGCGCTTCGACGCTGATCACGGGTTTTTTCTCAACGGACGTCCGCTCAAGATCAAGGGCGTCTGCCTGCATC
AGGACCATGCTGGCGTCGGC

Downstream 100 bases:

>100_bases
CCTTTTCACTGTTTTCCTCATCGTCGCCTGCGCGCCTGTTCGCCGTGGTGCTGGGCGCGTTGCTCGCCACTCCCGTCTCC
GTGCTGGCGGCATCGCCCGC

Product: truncated beta-galactosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MALPDALHVFRLQRLKSIGCNAIRLHHAVAPELLQACDRLGMLVMAENRLFNPAPDYVALLRNMVRRQRNHPSVFLWSLL
NEEPLQGTATGYAITARAASAVRALDDTRPITAGMNDGMFATGGAADVVDVLGFNYRQYNYDRVHAARPRTPMLSSEDTS
AFQTRGAWFTDLDAHVVAEDDSLAADWGNTHRRAWQLIAERPFVAGSFVWTGFDYRGEPTPFEWPSVGAFFGIMDQCGFA
KGAYWLRRALWIDDAPVLHLLPHWNWPGREGQPIKVMAFCNAEQVELWLNGRSLGKQAVDRAQSNQWQVDYAPGVLEAVA
WRGGHEVARTRVETTGAPVALKLTPDRSELHGDGRDAQPVTLEAVDAQGRHVPDCNALLTLQISGGRLLGIGNGDPNSHA
ADQSTQVALFNGLAQAIVQAGSGAGVLRLQASSPGLRDAVLDIGCTSAPLPASVPIAAPVMVVESWRHTAAFAQPPAPDL
PRRPNDNNSWSNTLPGTLESAPERAGYVLYRSQFTPWQGMQTRGGILDLGRLSGPAQVFLDGRAVAQAAAGTPVTVALAP
AAGERTLAVILQVAADQPFGFHDVVTVHYRNPP

Sequences:

>Translated_593_residues
MALPDALHVFRLQRLKSIGCNAIRLHHAVAPELLQACDRLGMLVMAENRLFNPAPDYVALLRNMVRRQRNHPSVFLWSLL
NEEPLQGTATGYAITARAASAVRALDDTRPITAGMNDGMFATGGAADVVDVLGFNYRQYNYDRVHAARPRTPMLSSEDTS
AFQTRGAWFTDLDAHVVAEDDSLAADWGNTHRRAWQLIAERPFVAGSFVWTGFDYRGEPTPFEWPSVGAFFGIMDQCGFA
KGAYWLRRALWIDDAPVLHLLPHWNWPGREGQPIKVMAFCNAEQVELWLNGRSLGKQAVDRAQSNQWQVDYAPGVLEAVA
WRGGHEVARTRVETTGAPVALKLTPDRSELHGDGRDAQPVTLEAVDAQGRHVPDCNALLTLQISGGRLLGIGNGDPNSHA
ADQSTQVALFNGLAQAIVQAGSGAGVLRLQASSPGLRDAVLDIGCTSAPLPASVPIAAPVMVVESWRHTAAFAQPPAPDL
PRRPNDNNSWSNTLPGTLESAPERAGYVLYRSQFTPWQGMQTRGGILDLGRLSGPAQVFLDGRAVAQAAAGTPVTVALAP
AAGERTLAVILQVAADQPFGFHDVVTVHYRNPP
>Mature_592_residues
ALPDALHVFRLQRLKSIGCNAIRLHHAVAPELLQACDRLGMLVMAENRLFNPAPDYVALLRNMVRRQRNHPSVFLWSLLN
EEPLQGTATGYAITARAASAVRALDDTRPITAGMNDGMFATGGAADVVDVLGFNYRQYNYDRVHAARPRTPMLSSEDTSA
FQTRGAWFTDLDAHVVAEDDSLAADWGNTHRRAWQLIAERPFVAGSFVWTGFDYRGEPTPFEWPSVGAFFGIMDQCGFAK
GAYWLRRALWIDDAPVLHLLPHWNWPGREGQPIKVMAFCNAEQVELWLNGRSLGKQAVDRAQSNQWQVDYAPGVLEAVAW
RGGHEVARTRVETTGAPVALKLTPDRSELHGDGRDAQPVTLEAVDAQGRHVPDCNALLTLQISGGRLLGIGNGDPNSHAA
DQSTQVALFNGLAQAIVQAGSGAGVLRLQASSPGLRDAVLDIGCTSAPLPASVPIAAPVMVVESWRHTAAFAQPPAPDLP
RRPNDNNSWSNTLPGTLESAPERAGYVLYRSQFTPWQGMQTRGGILDLGRLSGPAQVFLDGRAVAQAAAGTPVTVALAPA
AGERTLAVILQVAADQPFGFHDVVTVHYRNPP

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=239, Percent_Identity=27.6150627615063, Blast_Score=73, Evalue=9e-13,
Organism=Drosophila melanogaster, GI62471735, Length=243, Percent_Identity=25.9259259259259, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19922582, Length=243, Percent_Identity=25.9259259259259, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24655438, Length=243, Percent_Identity=25.9259259259259, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 64253; Mature: 64122

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00608 GLYCOSYL_HYDROL_F2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALPDALHVFRLQRLKSIGCNAIRLHHAVAPELLQACDRLGMLVMAENRLFNPAPDYVAL
CCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHH
LRNMVRRQRNHPSVFLWSLLNEEPLQGTATGYAITARAASAVRALDDTRPITAGMNDGMF
HHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCEECCCCCCEE
ATGGAADVVDVLGFNYRQYNYDRVHAARPRTPMLSSEDTSAFQTRGAWFTDLDAHVVAED
ECCCHHHHHHHHCCCCEECCCCCEECCCCCCCCCCCCCCHHHHHCCCEEECCCCEEEECC
DSLAADWGNTHRRAWQLIAERPFVAGSFVWTGFDYRGEPTPFEWPSVGAFFGIMDQCGFA
CCCCCCCCCHHHHHHHHHHCCCCEECEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCC
KGAYWLRRALWIDDAPVLHLLPHWNWPGREGQPIKVMAFCNAEQVELWLNGRSLGKQAVD
HHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCEEEEEEECCHHHHHHHHH
RAQSNQWQVDYAPGVLEAVAWRGGHEVARTRVETTGAPVALKLTPDRSELHGDGRDAQPV
HHCCCCEEEEECCCHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCHHHHCCCCCCCCCE
TLEAVDAQGRHVPDCNALLTLQISGGRLLGIGNGDPNSHAADQSTQVALFNGLAQAIVQA
EEEEECCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GSGAGVLRLQASSPGLRDAVLDIGCTSAPLPASVPIAAPVMVVESWRHTAAFAQPPAPDL
CCCCCEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
PRRPNDNNSWSNTLPGTLESAPERAGYVLYRSQFTPWQGMQTRGGILDLGRLSGPAQVFL
CCCCCCCCCCCCCCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCEEEEE
DGRAVAQAAAGTPVTVALAPAAGERTLAVILQVAADQPFGFHDVVTVHYRNPP
CCHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCC
>Mature Secondary Structure 
ALPDALHVFRLQRLKSIGCNAIRLHHAVAPELLQACDRLGMLVMAENRLFNPAPDYVAL
CCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHH
LRNMVRRQRNHPSVFLWSLLNEEPLQGTATGYAITARAASAVRALDDTRPITAGMNDGMF
HHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHCCCCCEECCCCCCEE
ATGGAADVVDVLGFNYRQYNYDRVHAARPRTPMLSSEDTSAFQTRGAWFTDLDAHVVAED
ECCCHHHHHHHHCCCCEECCCCCEECCCCCCCCCCCCCCHHHHHCCCEEECCCCEEEECC
DSLAADWGNTHRRAWQLIAERPFVAGSFVWTGFDYRGEPTPFEWPSVGAFFGIMDQCGFA
CCCCCCCCCHHHHHHHHHHCCCCEECEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCC
KGAYWLRRALWIDDAPVLHLLPHWNWPGREGQPIKVMAFCNAEQVELWLNGRSLGKQAVD
HHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCEEEEEECCCEEEEEEECCHHHHHHHHH
RAQSNQWQVDYAPGVLEAVAWRGGHEVARTRVETTGAPVALKLTPDRSELHGDGRDAQPV
HHCCCCEEEEECCCHHHHHHHCCCHHHHHHHHCCCCCCEEEEECCCHHHHCCCCCCCCCE
TLEAVDAQGRHVPDCNALLTLQISGGRLLGIGNGDPNSHAADQSTQVALFNGLAQAIVQA
EEEEECCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHC
GSGAGVLRLQASSPGLRDAVLDIGCTSAPLPASVPIAAPVMVVESWRHTAAFAQPPAPDL
CCCCCEEEEECCCCCHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
PRRPNDNNSWSNTLPGTLESAPERAGYVLYRSQFTPWQGMQTRGGILDLGRLSGPAQVFL
CCCCCCCCCCCCCCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCCCCEEEEE
DGRAVAQAAAGTPVTVALAPAAGERTLAVILQVAADQPFGFHDVVTVHYRNPP
CCHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA