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The map label for this gene is lacZ [H]

Identifier: 21241478

GI number: 21241478

Start: 838208

End: 839365

Strand: Direct

Name: lacZ [H]

Synonym: XAC0707

Alternate gene names: 21241478

Gene position: 838208-839365 (Clockwise)

Preceding gene: 21241477

Following gene: 21241479

Centisome position: 16.2

GC content: 66.93

Gene sequence:

>1158_bases
ATGCGCAGGCGTGAGTTCCTAGCCGGCAGCGTCTGCGCCTCATCGCTGCTTAGCGCGCCTGCGCTGGTCTCTGGCGCGGC
AGCCGCAGGCACCAGACGTGTCGGCACCAGCGCCAAGCCGCGTCCCAGGCCACAGGGCTCCGCGACAGATGCCGGCGGCC
AATGGCTGGATCTGGACGATGGGTGGCGCTTCCATGCCGGCGATGTGCCATTTCCGCGCCTGCTGGGGCAGGACGAGACG
TACGCCAATGGCAAGGCGGGCCGGGCCTGGGGCGCAGCGGCGCCGGAGTTCGACGATACCGGTTGGCGGCAGCTGCAAGT
GCCGCACGATTTTGTATTGGAGCAACCGGTGCGCGCCGACGCCAACGTCGCGCAAGGCTATCGCCCGCGCGGTATCGCCT
GGTACCGGCGCACGTTTCGGCTGGAAGAGGCAGTGCGCGACCAGGCGGTGGAACTGCGTCTGGACGGTGTCGCCAGCCAT
GCCACGGTGTGGGTCAACGGCATGCTGATGGCGCGCAGTTGGAGCGGCTACAACGGGCTGGTGATCGACATCACGCCCGT
CGCACGTTATGGCAATGCATTGAACTGCATCGCCATCCGCGTCGATGCCGAGGCAATGGATGGGTGGTGGTACGAAGGCG
GCGGCATCTACCGACACACCTGGCTGGTGCTGCGCGCGCCCCTGCATATCGCCGGCGACGGCCTGGCAGCCGTGCCGCGG
GAAGGCCCAGACGATGTTTGGCAGGTGCCGGTGCAGTTGGACCTTGTCAACAGCGGTGAACAGCCCGCTGATGCGTGGGT
CGAGGTCGTCTTGCGCGACACGCATGGCGCTGTCGTGGCGCATGGTGAGACACAACGACGCGTCGCTGCATTCGGCACGG
CACAGGCGGCGCTCAGCATCCAGTGCCGCAGCCGCAGCGCTGGAGCGTGGACACCCCCGTGCTGTATCGCCTGCAAGCCG
AAGTGCGCAGCGCGGATGGCCGCAGCGACCGTGCCGCATGCGAGGTCGGTTTTCGTAGCCTGCGCTTCGACGCTGATCAC
GGGTTTTTTCTCAACGGACGTCCGCTCAAGATCAAGGGCGTCTGCCTGCATCAGGACCATGCTGGCGTCGGCGTGGCGTT
GCCCGATGCGCTGCATGTGTTTCGTCTGCAGCGGCTGA

Upstream 100 bases:

>100_bases
AGTGAGGGCGACAGTGCGGTTTCGTTGGAAGCGTGCCATGCAGTGCTAGCAGTGCAGGCACCATGTGACGTGCATGCACT
GCAGTTGACCAGGCGCTGCC

Downstream 100 bases:

>100_bases
AATCGATTGGCTGCAACGCCATCCGTCTGCATCACGCAGTGGCGCCGGAACTGCTGCAGGCCTGCGACCGTCTCGGCATG
TTGGTGATGGCCGAGAACCG

Product: truncated beta-galactosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MRRREFLAGSVCASSLLSAPALVSGAAAAGTRRVGTSAKPRPRPQGSATDAGGQWLDLDDGWRFHAGDVPFPRLLGQDET
YANGKAGRAWGAAAPEFDDTGWRQLQVPHDFVLEQPVRADANVAQGYRPRGIAWYRRTFRLEEAVRDQAVELRLDGVASH
ATVWVNGMLMARSWSGYNGLVIDITPVARYGNALNCIAIRVDAEAMDGWWYEGGGIYRHTWLVLRAPLHIAGDGLAAVPR
EGPDDVWQVPVQLDLVNSGEQPADAWVEVVLRDTHGAVVAHGETQRRVAAFGTAQAALSIQCRSRSAGAWTPPCCIACKP
KCAARMAAATVPHARSVFVACASTLITGFFSTDVRSRSRASACIRTMLASAWRCPMRCMCFVCSG

Sequences:

>Translated_385_residues
MRRREFLAGSVCASSLLSAPALVSGAAAAGTRRVGTSAKPRPRPQGSATDAGGQWLDLDDGWRFHAGDVPFPRLLGQDET
YANGKAGRAWGAAAPEFDDTGWRQLQVPHDFVLEQPVRADANVAQGYRPRGIAWYRRTFRLEEAVRDQAVELRLDGVASH
ATVWVNGMLMARSWSGYNGLVIDITPVARYGNALNCIAIRVDAEAMDGWWYEGGGIYRHTWLVLRAPLHIAGDGLAAVPR
EGPDDVWQVPVQLDLVNSGEQPADAWVEVVLRDTHGAVVAHGETQRRVAAFGTAQAALSIQCRSRSAGAWTPPCCIACKP
KCAARMAAATVPHARSVFVACASTLITGFFSTDVRSRSRASACIRTMLASAWRCPMRCMCFVCSG
>Mature_385_residues
MRRREFLAGSVCASSLLSAPALVSGAAAAGTRRVGTSAKPRPRPQGSATDAGGQWLDLDDGWRFHAGDVPFPRLLGQDET
YANGKAGRAWGAAAPEFDDTGWRQLQVPHDFVLEQPVRADANVAQGYRPRGIAWYRRTFRLEEAVRDQAVELRLDGVASH
ATVWVNGMLMARSWSGYNGLVIDITPVARYGNALNCIAIRVDAEAMDGWWYEGGGIYRHTWLVLRAPLHIAGDGLAAVPR
EGPDDVWQVPVQLDLVNSGEQPADAWVEVVLRDTHGAVVAHGETQRRVAAFGTAQAALSIQCRSRSAGAWTPPCCIACKP
KCAARMAAATVPHARSVFVACASTLITGFFSTDVRSRSRASACIRTMLASAWRCPMRCMCFVCSG

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 41620; Mature: 41620

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRREFLAGSVCASSLLSAPALVSGAAAAGTRRVGTSAKPRPRPQGSATDAGGQWLDLDD
CCCHHHHHHHHHHHHHHCCCHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCC
GWRFHAGDVPFPRLLGQDETYANGKAGRAWGAAAPEFDDTGWRQLQVPHDFVLEQPVRAD
CCEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHCCCCCCC
ANVAQGYRPRGIAWYRRTFRLEEAVRDQAVELRLDGVASHATVWVNGMLMARSWSGYNGL
CHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEEEEEEEEEEECCCCCCE
VIDITPVARYGNALNCIAIRVDAEAMDGWWYEGGGIYRHTWLVLRAPLHIAGDGLAAVPR
EEEEECHHHCCCCEEEEEEEEECCCCCCEEECCCCEEEEEEEEEECCEEECCCCCEECCC
EGPDDVWQVPVQLDLVNSGEQPADAWVEVVLRDTHGAVVAHGETQRRVAAFGTAQAALSI
CCCCCCEECCEEEEEECCCCCHHHHHHEEEEECCCCEEEECCCCHHHHHHHCCCCEEEEE
QCRSRSAGAWTPPCCIACKPKCAARMAAATVPHARSVFVACASTLITGFFSTDVRSRSRA
EEECCCCCCCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SACIRTMLASAWRCPMRCMCFVCSG
HHHHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure
MRRREFLAGSVCASSLLSAPALVSGAAAAGTRRVGTSAKPRPRPQGSATDAGGQWLDLDD
CCCHHHHHHHHHHHHHHCCCHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCC
GWRFHAGDVPFPRLLGQDETYANGKAGRAWGAAAPEFDDTGWRQLQVPHDFVLEQPVRAD
CCEEECCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHCCCCCCC
ANVAQGYRPRGIAWYRRTFRLEEAVRDQAVELRLDGVASHATVWVNGMLMARSWSGYNGL
CHHHCCCCCCCHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEEEEEEEEEEECCCCCCE
VIDITPVARYGNALNCIAIRVDAEAMDGWWYEGGGIYRHTWLVLRAPLHIAGDGLAAVPR
EEEEECHHHCCCCEEEEEEEEECCCCCCEEECCCCEEEEEEEEEECCEEECCCCCEECCC
EGPDDVWQVPVQLDLVNSGEQPADAWVEVVLRDTHGAVVAHGETQRRVAAFGTAQAALSI
CCCCCCEECCEEEEEECCCCCHHHHHHEEEEECCCCEEEECCCCHHHHHHHCCCCEEEEE
QCRSRSAGAWTPPCCIACKPKCAARMAAATVPHARSVFVACASTLITGFFSTDVRSRSRA
EEECCCCCCCCCCEEEEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SACIRTMLASAWRCPMRCMCFVCSG
HHHHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA