Click here to switch to the map view.

The map label for this gene is ptrB [C]

Identifier: 21241399

GI number: 21241399

Start: 741741

End: 743834

Strand: Direct

Name: ptrB [C]

Synonym: XAC0628

Alternate gene names: 21241399

Gene position: 741741-743834 (Clockwise)

Preceding gene: 21241398

Following gene: 21241400

Centisome position: 14.33

GC content: 64.33

Gene sequence:

>2094_bases
ATGCCCAAGTTCGCCTCGATCTGCCTGGTTGCCGGCCTGATCACCGCTGGCGCCGTCTCTGCAGAGGAACCCCCCGTGTC
CAACGATCCCTACGCCTGGCTGGAAGATGTCACCGGCGCCAAGCCGCTGGATTGGGTCAAGACCCAGAACGCCAAGACCG
AGGCGCGGCTGGCCAGCACGCAGGCGTTCAAGCAGATGGAAACCAGCATCCGCGAAGTGCTGGATTCGGACGCCAAGATT
CCGGGCGTGCAGAAGATCGGCGCGTTCTACTACAACTTCTGGAAGGACAAGCAGCACGAGCGCGGCCTGTGGCGGCGCAC
CACGCTGGACGAGTACCGCAAGGCCACGCCCAAGTGGGAAACGGTGCTGGACCTGGATGTGCTCAACAAGGCCGAAGGCG
AAAACTGGGTGTGGCACGGTGCCGACTGCCTGCGCCCGGACTACCAGCGCTGCCTGATCGCGTTGTCGCGCGGCGGTGCC
GATGCCGATGTCACGCGCGAGTTCGATCTGGGCAGCAAGCAATGGGTCAAGGACGGCTTCTTCCGCCCCGAGTCCAAGGG
TGGGCTGGGCTGGATCGACCGGGACACTGTCTACGTGTTCACCGACTTCGGCGCCGGCAGCATGACCAGCTCCGGCTACC
CGCGCATCGCCAAGCAATGGAAGCGCGGCACGCCACTGACTGCCGCGACCGTGGTGTACGAAGGCAAGCCGACCGATATG
TACATCGCAGCCATGCATGACGACACGCCCGGATTCGAGCGCGATTTCGTCAGCCGCACGCTCGCCTTCTACAACGACGA
GCTGTATCTCAAGGGCGCCGACGGCACGCTGGTCAAGGTGGACGTGCCCAATTCGGCCAGCAAGTCGGTCAAGCGCCAGT
GGCTGACGCTGGAGCTGCGCGAACCGTGGACGGTCGGCGGCAAGACCTACAAGCCCGGCACCTTGCTGGCCACGCGTTTC
GACGATTTCATGGCAGGCAAACGCAACTTCGATGTGTTGTTCGAGCCCACCGACACCACCTCGCTGGCCGGCATGGCGTG
GACCAAGTCGCATCTGGTATTGAACGTGCTGGAAGACGTCAAGAACCGTCTGAGCGTGACCACACCCTCGCCCGATGGTT
GGAAAAAGAGCGCGTTTGTCGGTGCGCCGAGCTTCGGTACGGTGGATGTCGGCGCGGTGGATAGCGACGACAGCGACGCG
CTGTGGCTCACCGTCACCGATTATCTGACCCCCACCACCTTGTCGTGGGTGGACGTGGGCGCTGCGCCGCAGCCGCTCAA
GACCATGCCGGCGTTCTTCGATGCCGGCAAGGACACCATCGAGCAGCACTTCGCCACCAGCAAGGACGGCACCCGCGTGC
CGTATTTCCTGGTACGCCCGAAGGCCCTGAAGCTCGACGGCAGCGCGCCGACCTTGCTGTACGGCTACGGCGGTTTCGAA
ATTTCGATGACGCCCAATTACTCGGGCGGCCTGGGGCGCGCCTGGCTGGAGAAGGGCGGCGTCTACGTGGTGGCCAATAT
CCGCGGCGGTGGCGAATACGGCCCACGCTGGCACCAGGCCGCGCTCAAGCAGAATCGCCACAAGGCCTACGAGGACATGG
CCGCGGTGGCCAAGGATCTGGTGGCGCGCAAGATCACCTCGACCAAGCATCTGGGCGTGCAAGGCGGCAGCAATGGCGGC
CTGATGACCGGCAACATGCTCACCCAGTATCCGGAGCTGTTCGGCGCGGTGGTGGTGCAGGTGCCGCTGCTGGACATGAA
GCGCTACAGCCACCTGCTGGCCGGCGCCTCGTGGATGGCCGAATACGGCAACCCCGACACCGACGACTGGACGTTCATCC
AGACCTTCTCGCCGTACCACCTGTTCGATGCCAAGAAGACCTACCCACCGGTGATCTTCCTCACCTCCACGCGCGACGAC
CGCGTGCATCCCGGCCACGCGCGCAAGATGGCCGCAAAGATGATCGAGGCCGGCAAGGACGTGACCTATTACGAGAACAT
CGAAGGTGGCCATGGCGGTGCGGCCAATAACGCGCAGGCCGCGCACATGGCCGCGCTGGCGTATAGCTTCCTGTGGGAGC
GGTTGGCCGACTGA

Upstream 100 bases:

>100_bases
TCGCGTACGGCGACACAGGCGCGCGCAGCCTGACCTTCGGCAGGGTTGGCCGACGGGGCGCTGTGCTACGTTCCGGGCAT
TCGTTTTTCCGGAAACATTC

Downstream 100 bases:

>100_bases
GCGATCATTGCCGGTTGCGCTGCGTTGATCGATACCGGCCCGCTGGCAGTGCACAGCGGGCCGGATCGTTTGCGACCGGT
GGGTATTGCCGGTGTCTGGA

Product: prolyl oligopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 697; Mature: 696

Protein sequence:

>697_residues
MPKFASICLVAGLITAGAVSAEEPPVSNDPYAWLEDVTGAKPLDWVKTQNAKTEARLASTQAFKQMETSIREVLDSDAKI
PGVQKIGAFYYNFWKDKQHERGLWRRTTLDEYRKATPKWETVLDLDVLNKAEGENWVWHGADCLRPDYQRCLIALSRGGA
DADVTREFDLGSKQWVKDGFFRPESKGGLGWIDRDTVYVFTDFGAGSMTSSGYPRIAKQWKRGTPLTAATVVYEGKPTDM
YIAAMHDDTPGFERDFVSRTLAFYNDELYLKGADGTLVKVDVPNSASKSVKRQWLTLELREPWTVGGKTYKPGTLLATRF
DDFMAGKRNFDVLFEPTDTTSLAGMAWTKSHLVLNVLEDVKNRLSVTTPSPDGWKKSAFVGAPSFGTVDVGAVDSDDSDA
LWLTVTDYLTPTTLSWVDVGAAPQPLKTMPAFFDAGKDTIEQHFATSKDGTRVPYFLVRPKALKLDGSAPTLLYGYGGFE
ISMTPNYSGGLGRAWLEKGGVYVVANIRGGGEYGPRWHQAALKQNRHKAYEDMAAVAKDLVARKITSTKHLGVQGGSNGG
LMTGNMLTQYPELFGAVVVQVPLLDMKRYSHLLAGASWMAEYGNPDTDDWTFIQTFSPYHLFDAKKTYPPVIFLTSTRDD
RVHPGHARKMAAKMIEAGKDVTYYENIEGGHGGAANNAQAAHMAALAYSFLWERLAD

Sequences:

>Translated_697_residues
MPKFASICLVAGLITAGAVSAEEPPVSNDPYAWLEDVTGAKPLDWVKTQNAKTEARLASTQAFKQMETSIREVLDSDAKI
PGVQKIGAFYYNFWKDKQHERGLWRRTTLDEYRKATPKWETVLDLDVLNKAEGENWVWHGADCLRPDYQRCLIALSRGGA
DADVTREFDLGSKQWVKDGFFRPESKGGLGWIDRDTVYVFTDFGAGSMTSSGYPRIAKQWKRGTPLTAATVVYEGKPTDM
YIAAMHDDTPGFERDFVSRTLAFYNDELYLKGADGTLVKVDVPNSASKSVKRQWLTLELREPWTVGGKTYKPGTLLATRF
DDFMAGKRNFDVLFEPTDTTSLAGMAWTKSHLVLNVLEDVKNRLSVTTPSPDGWKKSAFVGAPSFGTVDVGAVDSDDSDA
LWLTVTDYLTPTTLSWVDVGAAPQPLKTMPAFFDAGKDTIEQHFATSKDGTRVPYFLVRPKALKLDGSAPTLLYGYGGFE
ISMTPNYSGGLGRAWLEKGGVYVVANIRGGGEYGPRWHQAALKQNRHKAYEDMAAVAKDLVARKITSTKHLGVQGGSNGG
LMTGNMLTQYPELFGAVVVQVPLLDMKRYSHLLAGASWMAEYGNPDTDDWTFIQTFSPYHLFDAKKTYPPVIFLTSTRDD
RVHPGHARKMAAKMIEAGKDVTYYENIEGGHGGAANNAQAAHMAALAYSFLWERLAD
>Mature_696_residues
PKFASICLVAGLITAGAVSAEEPPVSNDPYAWLEDVTGAKPLDWVKTQNAKTEARLASTQAFKQMETSIREVLDSDAKIP
GVQKIGAFYYNFWKDKQHERGLWRRTTLDEYRKATPKWETVLDLDVLNKAEGENWVWHGADCLRPDYQRCLIALSRGGAD
ADVTREFDLGSKQWVKDGFFRPESKGGLGWIDRDTVYVFTDFGAGSMTSSGYPRIAKQWKRGTPLTAATVVYEGKPTDMY
IAAMHDDTPGFERDFVSRTLAFYNDELYLKGADGTLVKVDVPNSASKSVKRQWLTLELREPWTVGGKTYKPGTLLATRFD
DFMAGKRNFDVLFEPTDTTSLAGMAWTKSHLVLNVLEDVKNRLSVTTPSPDGWKKSAFVGAPSFGTVDVGAVDSDDSDAL
WLTVTDYLTPTTLSWVDVGAAPQPLKTMPAFFDAGKDTIEQHFATSKDGTRVPYFLVRPKALKLDGSAPTLLYGYGGFEI
SMTPNYSGGLGRAWLEKGGVYVVANIRGGGEYGPRWHQAALKQNRHKAYEDMAAVAKDLVARKITSTKHLGVQGGSNGGL
MTGNMLTQYPELFGAVVVQVPLLDMKRYSHLLAGASWMAEYGNPDTDDWTFIQTFSPYHLFDAKKTYPPVIFLTSTRDDR
VHPGHARKMAAKMIEAGKDVTYYENIEGGHGGAANNAQAAHMAALAYSFLWERLAD

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=377, Percent_Identity=32.0954907161804, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI284172438, Length=226, Percent_Identity=25.2212389380531, Blast_Score=73, Evalue=9e-13,
Organism=Homo sapiens, GI284172431, Length=226, Percent_Identity=25.2212389380531, Blast_Score=73, Evalue=9e-13,
Organism=Homo sapiens, GI284172420, Length=226, Percent_Identity=25.2212389380531, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI284172413, Length=226, Percent_Identity=25.2212389380531, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI70778815, Length=226, Percent_Identity=25.2212389380531, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI108860686, Length=226, Percent_Identity=25.2212389380531, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI108860692, Length=223, Percent_Identity=24.6636771300448, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788150, Length=693, Percent_Identity=23.0880230880231, Blast_Score=122, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24583414, Length=374, Percent_Identity=32.620320855615, Blast_Score=189, Evalue=7e-48,
Organism=Drosophila melanogaster, GI221510989, Length=289, Percent_Identity=33.2179930795848, Blast_Score=162, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 77072; Mature: 76940

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS01072 SLH_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKFASICLVAGLITAGAVSAEEPPVSNDPYAWLEDVTGAKPLDWVKTQNAKTEARLAST
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHCCCCCHHHHHHHH
QAFKQMETSIREVLDSDAKIPGVQKIGAFYYNFWKDKQHERGLWRRTTLDEYRKATPKWE
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHCCCHHHCCHHHHHHCCCCHH
TVLDLDVLNKAEGENWVWHGADCLRPDYQRCLIALSRGGADADVTREFDLGSKQWVKDGF
EEEEHHHHHCCCCCCEEEECHHHCCHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHCCC
FRPESKGGLGWIDRDTVYVFTDFGAGSMTSSGYPRIAKQWKRGTPLTAATVVYEGKPTDM
CCCCCCCCCCEEECCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCE
YIAAMHDDTPGFERDFVSRTLAFYNDELYLKGADGTLVKVDVPNSASKSVKRQWLTLELR
EEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCHHHHHHHEEEEEEEC
EPWTVGGKTYKPGTLLATRFDDFMAGKRNFDVLFEPTDTTSLAGMAWTKSHLVLNVLEDV
CCEECCCCEECCCCEEEEHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
KNRLSVTTPSPDGWKKSAFVGAPSFGTVDVGAVDSDDSDALWLTVTDYLTPTTLSWVDVG
HHHEEECCCCCCCCCCCEEECCCCCCCEEECCCCCCCCCEEEEEEECCCCCCEEEEEECC
AAPQPLKTMPAFFDAGKDTIEQHFATSKDGTRVPYFLVRPKALKLDGSAPTLLYGYGGFE
CCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEECCEEEEECCCCCEEEEECCCEE
ISMTPNYSGGLGRAWLEKGGVYVVANIRGGGEYGPRWHQAALKQNRHKAYEDMAAVAKDL
EEECCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VARKITSTKHLGVQGGSNGGLMTGNMLTQYPELFGAVVVQVPLLDMKRYSHLLAGASWMA
HHHHHCCCCEECCCCCCCCCEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EYGNPDTDDWTFIQTFSPYHLFDAKKTYPPVIFLTSTRDDRVHPGHARKMAAKMIEAGKD
HCCCCCCCCEEEEEECCCEEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCC
VTYYENIEGGHGGAANNAQAAHMAALAYSFLWERLAD
CEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PKFASICLVAGLITAGAVSAEEPPVSNDPYAWLEDVTGAKPLDWVKTQNAKTEARLAST
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHCCCCCHHHHHHHH
QAFKQMETSIREVLDSDAKIPGVQKIGAFYYNFWKDKQHERGLWRRTTLDEYRKATPKWE
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHCCCHHHCCHHHHHHCCCCHH
TVLDLDVLNKAEGENWVWHGADCLRPDYQRCLIALSRGGADADVTREFDLGSKQWVKDGF
EEEEHHHHHCCCCCCEEEECHHHCCHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHCCC
FRPESKGGLGWIDRDTVYVFTDFGAGSMTSSGYPRIAKQWKRGTPLTAATVVYEGKPTDM
CCCCCCCCCCEEECCEEEEEEECCCCCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCE
YIAAMHDDTPGFERDFVSRTLAFYNDELYLKGADGTLVKVDVPNSASKSVKRQWLTLELR
EEEEEECCCCCHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCHHHHHHHEEEEEEEC
EPWTVGGKTYKPGTLLATRFDDFMAGKRNFDVLFEPTDTTSLAGMAWTKSHLVLNVLEDV
CCEECCCCEECCCCEEEEHHHHHHCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHH
KNRLSVTTPSPDGWKKSAFVGAPSFGTVDVGAVDSDDSDALWLTVTDYLTPTTLSWVDVG
HHHEEECCCCCCCCCCCEEECCCCCCCEEECCCCCCCCCEEEEEEECCCCCCEEEEEECC
AAPQPLKTMPAFFDAGKDTIEQHFATSKDGTRVPYFLVRPKALKLDGSAPTLLYGYGGFE
CCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEECCEEEEECCCCCEEEEECCCEE
ISMTPNYSGGLGRAWLEKGGVYVVANIRGGGEYGPRWHQAALKQNRHKAYEDMAAVAKDL
EEECCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VARKITSTKHLGVQGGSNGGLMTGNMLTQYPELFGAVVVQVPLLDMKRYSHLLAGASWMA
HHHHHCCCCEECCCCCCCCCEEECHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
EYGNPDTDDWTFIQTFSPYHLFDAKKTYPPVIFLTSTRDDRVHPGHARKMAAKMIEAGKD
HCCCCCCCCEEEEEECCCEEEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHCCCC
VTYYENIEGGHGGAANNAQAAHMAALAYSFLWERLAD
CEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.47 {tosyl-Lys-methyl} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]