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The map label for this gene is pdhB [H]

Identifier: 21241216

GI number: 21241216

Start: 528796

End: 530313

Strand: Reverse

Name: pdhB [H]

Synonym: XAC0443

Alternate gene names: 21241216

Gene position: 530313-528796 (Counterclockwise)

Preceding gene: 21241217

Following gene: 21241215

Centisome position: 10.25

GC content: 70.03

Gene sequence:

>1518_bases
ATGAGCGACAACAAGAATTTCCACCTGCCCGACCTGGGCGAAGGCCTGCCCGACGCCACCATCGTCGAATGGTTCGTCAA
GGAGGGCGACACCGTGCGCCTGGACGACCCGCTGGTGTCGATGGAAACCGCCAAGGCGGTGGTCGAAGTGCCCTCGCCGT
TTTCCGGCACCGTGGTCAAGCTCGCCGGCGCTGCCGGGGATGTGATCGTGACCGGCTCGGTGCTGGCGCAGTTCGCGCTG
GATGCCTCGCAGCCGCAGCGTGCCGACGGGCAGGACACCGGTCATTCGCATGGCCCGGCGCCGACCCACTCCCCCACGCC
CAGCACCGGCGACAGTGCGGCCGGCCCCACTGCGCGCGTGGTCGCCTCCGACAACGGCGGCGAGATTGCCGATGCCGATG
CTGGTGATGAGGGCAGCGGCGATCGCGACGATGCCGGCACCGTGGTCGGTGCGATGCAGAGCTCCAACGCAGTGCAGAGC
GAGCAGGCCATCGCCGTCGGCGGCGTGCGCGCCATGCCGGTGGTGCGCGCGCTGGCGCGCAAGTTGCGCGTGGAGCTGGC
ACAGGTGCGCGCCACCGGGCCGGACGGCACGGTCACGCTGGCAGACGTAAAGCAGGCAGCGGCCGCCGGCACCGCGCAGC
CCTCACCCGGCGTGCAGGGCGCGCCGAGCTCTCCCGCCAACGGGAAACGCGGTCTGATCGGTGACGGTGGGAGAGCCGCA
GGGATCGCTGCCGACGGCAGGGATCGACCCGCAACAGCGGCCCTTCTTCCGTCTGCGGGAGAAGGTGCCCGCAGGGCGGA
TGTGGGCGGCGCCGCTGCCCGCTCGCCGCTCTCGGCCAGCGGCAAGCCGATGCGCACCCAGTCGCCCGGCATCAGCGCCA
AAGGCCAACCCGAACAGCTCAAGGGCGTGCGTCGCAACATGGCACGCGTGATGGCCGATGCACACACCAAGGTGGTGCCG
ACCACGCTCAACGACGATGCCGATCTGCACGCCTGGCAGCCCGGCAACGATGTCACCGTGCGTCTGGTGCGTGGCATCGT
GCGCGCCTGCCAGGCGGTGCCCGCACTCAATGCCTGGTTCGACGGCGACGCCCTCAGCCGCACCCTGCACCACCAGGTCG
ACATCGGCATTGCCGTGGATACCGAGGAAGGCTTGTTCGTGCCGGCCTTGCGCAATGCCGACATGCTGGACGCGCATGGC
ATCCGCGAAGGCGTCAACCGGCTGCGCCAGCAGGTGGAAAGCCGCAGCATCGCCGCCTCCGAACTGAGCGGCTACACCAT
CTCATTGTCCAACTTCGGCATGTTCGCCGGCCGCTACGCCACCCCGGTGGTGGTGCCGCCCTGCGTGGCGATCGTGGCCG
CCGGCCGCGCGCGCTACCAGCTCACCCCGGTGATGGGCGGGGTGGAAACCCACAAGGTGATGCCGCTGTCGCTGACCTTC
GATCACCGCGCCGCCACCGGCGGTGAAGCAGCGCGCTTCCTGCGCGCCTTGCTGGACGATCTGGCGTTGGCCAACTAG

Upstream 100 bases:

>100_bases
CCGAGCGTGCCGACGGCACGCAGGGCTGGCTGCCGGCCGCCGACCTTGAACTCCTCGACGACACCACGCCGCAGCTGCCT
GCCGCGCAAGAGAATCTGCC

Downstream 100 bases:

>100_bases
GCGCGCCTCACGAACGACTGCGCTCACCGCCAGGTGGGCGCAGCCGGCCTTGTATTTCCATCGCCACACGAACACGTTGG
CGGGTGCGGTGCCCTCGCCG

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MSDNKNFHLPDLGEGLPDATIVEWFVKEGDTVRLDDPLVSMETAKAVVEVPSPFSGTVVKLAGAAGDVIVTGSVLAQFAL
DASQPQRADGQDTGHSHGPAPTHSPTPSTGDSAAGPTARVVASDNGGEIADADAGDEGSGDRDDAGTVVGAMQSSNAVQS
EQAIAVGGVRAMPVVRALARKLRVELAQVRATGPDGTVTLADVKQAAAAGTAQPSPGVQGAPSSPANGKRGLIGDGGRAA
GIAADGRDRPATAALLPSAGEGARRADVGGAAARSPLSASGKPMRTQSPGISAKGQPEQLKGVRRNMARVMADAHTKVVP
TTLNDDADLHAWQPGNDVTVRLVRGIVRACQAVPALNAWFDGDALSRTLHHQVDIGIAVDTEEGLFVPALRNADMLDAHG
IREGVNRLRQQVESRSIAASELSGYTISLSNFGMFAGRYATPVVVPPCVAIVAAGRARYQLTPVMGGVETHKVMPLSLTF
DHRAATGGEAARFLRALLDDLALAN

Sequences:

>Translated_505_residues
MSDNKNFHLPDLGEGLPDATIVEWFVKEGDTVRLDDPLVSMETAKAVVEVPSPFSGTVVKLAGAAGDVIVTGSVLAQFAL
DASQPQRADGQDTGHSHGPAPTHSPTPSTGDSAAGPTARVVASDNGGEIADADAGDEGSGDRDDAGTVVGAMQSSNAVQS
EQAIAVGGVRAMPVVRALARKLRVELAQVRATGPDGTVTLADVKQAAAAGTAQPSPGVQGAPSSPANGKRGLIGDGGRAA
GIAADGRDRPATAALLPSAGEGARRADVGGAAARSPLSASGKPMRTQSPGISAKGQPEQLKGVRRNMARVMADAHTKVVP
TTLNDDADLHAWQPGNDVTVRLVRGIVRACQAVPALNAWFDGDALSRTLHHQVDIGIAVDTEEGLFVPALRNADMLDAHG
IREGVNRLRQQVESRSIAASELSGYTISLSNFGMFAGRYATPVVVPPCVAIVAAGRARYQLTPVMGGVETHKVMPLSLTF
DHRAATGGEAARFLRALLDDLALAN
>Mature_504_residues
SDNKNFHLPDLGEGLPDATIVEWFVKEGDTVRLDDPLVSMETAKAVVEVPSPFSGTVVKLAGAAGDVIVTGSVLAQFALD
ASQPQRADGQDTGHSHGPAPTHSPTPSTGDSAAGPTARVVASDNGGEIADADAGDEGSGDRDDAGTVVGAMQSSNAVQSE
QAIAVGGVRAMPVVRALARKLRVELAQVRATGPDGTVTLADVKQAAAAGTAQPSPGVQGAPSSPANGKRGLIGDGGRAAG
IAADGRDRPATAALLPSAGEGARRADVGGAAARSPLSASGKPMRTQSPGISAKGQPEQLKGVRRNMARVMADAHTKVVPT
TLNDDADLHAWQPGNDVTVRLVRGIVRACQAVPALNAWFDGDALSRTLHHQVDIGIAVDTEEGLFVPALRNADMLDAHGI
REGVNRLRQQVESRSIAASELSGYTISLSNFGMFAGRYATPVVVPPCVAIVAAGRARYQLTPVMGGVETHKVMPLSLTFD
HRAATGGEAARFLRALLDDLALAN

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=215, Percent_Identity=25.5813953488372, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI110671329, Length=226, Percent_Identity=29.2035398230088, Blast_Score=86, Evalue=8e-17,
Organism=Homo sapiens, GI203098816, Length=252, Percent_Identity=26.1904761904762, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI203098753, Length=252, Percent_Identity=26.1904761904762, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI31711992, Length=156, Percent_Identity=29.4871794871795, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1786946, Length=161, Percent_Identity=31.6770186335404, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1786305, Length=166, Percent_Identity=27.1084337349398, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25146366, Length=156, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17560088, Length=162, Percent_Identity=32.0987654320988, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17538894, Length=162, Percent_Identity=29.6296296296296, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17537937, Length=217, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320352, Length=155, Percent_Identity=30.9677419354839, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324258, Length=211, Percent_Identity=26.5402843601896, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24645909, Length=149, Percent_Identity=32.8859060402685, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24582497, Length=162, Percent_Identity=31.4814814814815, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI20129315, Length=164, Percent_Identity=31.0975609756098, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI18859875, Length=214, Percent_Identity=27.1028037383178, Blast_Score=65, Evalue=1e-10,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 51781; Mature: 51649

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNKNFHLPDLGEGLPDATIVEWFVKEGDTVRLDDPLVSMETAKAVVEVPSPFSGTVVK
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHEEECCCCCCCCEEE
LAGAAGDVIVTGSVLAQFALDASQPQRADGQDTGHSHGPAPTHSPTPSTGDSAAGPTARV
EECCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
VASDNGGEIADADAGDEGSGDRDDAGTVVGAMQSSNAVQSEQAIAVGGVRAMPVVRALAR
EEECCCCCCCCCCCCCCCCCCCCCCCCEEEEHHCCCCHHCCCEEEECCCHHHHHHHHHHH
KLRVELAQVRATGPDGTVTLADVKQAAAAGTAQPSPGVQGAPSSPANGKRGLIGDGGRAA
HHHHHHHHHEECCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GIAADGRDRPATAALLPSAGEGARRADVGGAAARSPLSASGKPMRTQSPGISAKGQPEQL
CEECCCCCCCCEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
KGVRRNMARVMADAHTKVVPTTLNDDADLHAWQPGNDVTVRLVRGIVRACQAVPALNAWF
HHHHHHHHHHHHHCCCEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCC
DGDALSRTLHHQVDIGIAVDTEEGLFVPALRNADMLDAHGIREGVNRLRQQVESRSIAAS
CCHHHHHHHHHHEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ELSGYTISLSNFGMFAGRYATPVVVPPCVAIVAAGRARYQLTPVMGGVETHKVMPLSLTF
HCCCCEEEECCCCEECCCCCCCEECHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEE
DHRAATGGEAARFLRALLDDLALAN
CCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDNKNFHLPDLGEGLPDATIVEWFVKEGDTVRLDDPLVSMETAKAVVEVPSPFSGTVVK
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHEEECCCCCCCCEEE
LAGAAGDVIVTGSVLAQFALDASQPQRADGQDTGHSHGPAPTHSPTPSTGDSAAGPTARV
EECCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
VASDNGGEIADADAGDEGSGDRDDAGTVVGAMQSSNAVQSEQAIAVGGVRAMPVVRALAR
EEECCCCCCCCCCCCCCCCCCCCCCCCEEEEHHCCCCHHCCCEEEECCCHHHHHHHHHHH
KLRVELAQVRATGPDGTVTLADVKQAAAAGTAQPSPGVQGAPSSPANGKRGLIGDGGRAA
HHHHHHHHHEECCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GIAADGRDRPATAALLPSAGEGARRADVGGAAARSPLSASGKPMRTQSPGISAKGQPEQL
CEECCCCCCCCEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHH
KGVRRNMARVMADAHTKVVPTTLNDDADLHAWQPGNDVTVRLVRGIVRACQAVPALNAWF
HHHHHHHHHHHHHCCCEEEEEECCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCC
DGDALSRTLHHQVDIGIAVDTEEGLFVPALRNADMLDAHGIREGVNRLRQQVESRSIAAS
CCHHHHHHHHHHEEEEEEEECCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ELSGYTISLSNFGMFAGRYATPVVVPPCVAIVAAGRARYQLTPVMGGVETHKVMPLSLTF
HCCCCEEEECCCCEECCCCCCCEECHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEE
DHRAATGGEAARFLRALLDDLALAN
CCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA