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The map label for this gene is glgX [H]

Identifier: 21241204

GI number: 21241204

Start: 511906

End: 514068

Strand: Direct

Name: glgX [H]

Synonym: XAC0431

Alternate gene names: 21241204

Gene position: 511906-514068 (Clockwise)

Preceding gene: 21241203

Following gene: 21241205

Centisome position: 9.89

GC content: 64.12

Gene sequence:

>2163_bases
ATGGCCACTCGCAAGTTCACCCAGCGCTCGCGCATTCGCGAAGGCCGTCCCAACCCGCTCGGTGCCACCTGGGATGGGTT
GGGCGTCAACTTCGCGCTGTATTCGCGCAATGCCACCCGGGTGGAGCTGTGTCTGTTCGACGAACGTGGCCGCGAACAGG
AGCGCATTGCGCTCCCCGAATACACCGACGAGGTCTGGCACGGCTACCTGCCCGATGCACGCCCTGGCCAGTTGTACGGC
TACCGCGTACACGGCCCATATGCGCCCGATGCCGGGCACCGCTTCAATCACAATAAATTGTTGCTGGACCCGTATGCCAA
GCAGATCGTTGGCGAACTCAAATGGGCGCCGCACCTGTTCGGCTACACCATCGGTCATCGCGACAAGGATCTGAGCTTCG
ACCGCCGCGACAGCGCCGCGTTCATGCCCAAGTCTGCGGTGATCGACCCGGCTTTCACCTGGGGCCAGGACCGCCCGCCG
CAGACGCCGTGGAACCGCACGGTGATCTACGAAGCGCACGTACGCGGCCTGAGCATGTTGCACCCGGCGGTGCCGCCGGA
AGAGCGCGGCACCTTTTCGGCGCTGAAAACCGACGAATTGATCGACCACATCAGTTCGCTCGGCGTCACCGCAGTGGAAT
TGCTGCCGGTACATGCATTCGTCGACGATCAGTATCTGCTGGAAAAAGGCCTGCGCAATTACTGGGGCTACAACACGCTG
GGCTTTTTTGCCCCGCAGGCGCGTTACATGTCCACGCGCACCGTGGCCGAGTTCAAGCAAATGGTGGCGCGCCTGCATCA
CGCCGGCCTGGAAGTGCTGCTGGACGTGGTCTACAACCACACTGCCGAAGGCAACGAGCTCGGGCCCACGCTCTCGTTCA
AGGGGATCGACAACGCCAGCTATTACCGCCTGGCCGACGATCGCCGCTTCTACATCAACGATACCGGTACCGGTAACACC
TTCGACCTCACCAATGTCGGCGCGCTGCGCATGGTGATGGATTCGTTGCGTTACTGGGTACAGGAAATGCACGTGGACGG
CTTCCGTTTCGACCTGGCCAGCATCCTGGGCCGCGAACGCTATGGCTTCGATCCGTCCGGCAGTTTCCTGGATGCGGTGC
GCCAGGATCCGGTGCTCAGCCAGACCAAGTTGATCGCCGAGCCGTGGGATATCGGACCCGGCGGTTATCAGGTAGGCAAC
TTCCCGCCCGGCTGGGTGGAATGGAACGACAAGTTCCGCGACAACGTGCGCGCGTTCTGGCGCGGCGACGGCGGCCAGCT
GGCCGAGTTGGCCACGCGCCTCACCGGTTCTGCGGACCTGTTCGATCACAGCGGCCGCCGCCCAACCGCATCGGTCAATT
TCGTCACCGCGCATGACGGCTTCACCCTGCGCGATCTGGTCAGCTACGAAGGCAAGCACAACCTCGCCAATGGCGAAGAC
GGCAGGGACGGCAGCGACCACAACATCTCCTCCAACTATGGCGTGGAAGGCGAGACCAACGACCCGGCGATCAAGCAGCT
GCGTCGCCAGCAGATGCGCAACCTGCTCGCCACCTTGCTGTTGTCGCAGGGCACGCCGATGCTGCTGGCCGGCGATGAGT
TCGGCCATAGCCAGAACGGCAACAACAACGCGTATTGCCAGGACAACGAGCTGACCTGGATCGACTGGACCGCCGCCACC
AAGGCCGCAGCCGCCGACCAGGCCGCATTCGTGCGCCGATTGATCCGCATCCGGCAGCGCTACCCGCTGCTGCATCGCGC
GCGTTTCTTCGACGGCAAGTTCGACGAAGCGCTAGGCCTGAAGGATCTGACCTGGCTGGCGCCCAATGGCACCGAGATGG
ATGAAGCCGGCTGGCACGACCCGGAAGCACGCGCGCTGATGCTGCGTCTGGATGGTCGCTCGCCGACCACCGGCCTGCGC
GAGATTGCCGCCAACGTGACGCTGTTGATGCTGATCAATGCCGCCTCCACCGCCGTGTCGTTTACGCTGCCGGCCATGCA
CGACGAACATTGGCGCGTGCTGGTGGACACCGCACGCCACGGCGGCCGCGTGGTGGCCGGTGGCAGCGAATGGAAGGCCC
CGGCGCATTCGCTGACGCTGCTGGCCGTGGAGCGCGACCGCATCGTCAGCAGTGCGCGGATCGTCTCCGAAGGAGCGCGC
TGA

Upstream 100 bases:

>100_bases
TGCATGGGCCGGCCTGTTGCGGGCCGGGCCATGTGCTGCGGATCGCTCCGCCAGTGCAAAATCGCGCACCGTTTGCGTTC
ACCGTTCTAAGGAGTCACCG

Downstream 100 bases:

>100_bases
TGGCGAGGATGGCCTGATGGATGTCCTGTTGGCAGGGGCGACCGGCCTGGTCGGCAGGCACGTGCTGCAGCAATTGCTGG
GCGATACGCGTTGCACCGGC

Product: glycogen debranching enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 720; Mature: 719

Protein sequence:

>720_residues
MATRKFTQRSRIREGRPNPLGATWDGLGVNFALYSRNATRVELCLFDERGREQERIALPEYTDEVWHGYLPDARPGQLYG
YRVHGPYAPDAGHRFNHNKLLLDPYAKQIVGELKWAPHLFGYTIGHRDKDLSFDRRDSAAFMPKSAVIDPAFTWGQDRPP
QTPWNRTVIYEAHVRGLSMLHPAVPPEERGTFSALKTDELIDHISSLGVTAVELLPVHAFVDDQYLLEKGLRNYWGYNTL
GFFAPQARYMSTRTVAEFKQMVARLHHAGLEVLLDVVYNHTAEGNELGPTLSFKGIDNASYYRLADDRRFYINDTGTGNT
FDLTNVGALRMVMDSLRYWVQEMHVDGFRFDLASILGRERYGFDPSGSFLDAVRQDPVLSQTKLIAEPWDIGPGGYQVGN
FPPGWVEWNDKFRDNVRAFWRGDGGQLAELATRLTGSADLFDHSGRRPTASVNFVTAHDGFTLRDLVSYEGKHNLANGED
GRDGSDHNISSNYGVEGETNDPAIKQLRRQQMRNLLATLLLSQGTPMLLAGDEFGHSQNGNNNAYCQDNELTWIDWTAAT
KAAAADQAAFVRRLIRIRQRYPLLHRARFFDGKFDEALGLKDLTWLAPNGTEMDEAGWHDPEARALMLRLDGRSPTTGLR
EIAANVTLLMLINAASTAVSFTLPAMHDEHWRVLVDTARHGGRVVAGGSEWKAPAHSLTLLAVERDRIVSSARIVSEGAR

Sequences:

>Translated_720_residues
MATRKFTQRSRIREGRPNPLGATWDGLGVNFALYSRNATRVELCLFDERGREQERIALPEYTDEVWHGYLPDARPGQLYG
YRVHGPYAPDAGHRFNHNKLLLDPYAKQIVGELKWAPHLFGYTIGHRDKDLSFDRRDSAAFMPKSAVIDPAFTWGQDRPP
QTPWNRTVIYEAHVRGLSMLHPAVPPEERGTFSALKTDELIDHISSLGVTAVELLPVHAFVDDQYLLEKGLRNYWGYNTL
GFFAPQARYMSTRTVAEFKQMVARLHHAGLEVLLDVVYNHTAEGNELGPTLSFKGIDNASYYRLADDRRFYINDTGTGNT
FDLTNVGALRMVMDSLRYWVQEMHVDGFRFDLASILGRERYGFDPSGSFLDAVRQDPVLSQTKLIAEPWDIGPGGYQVGN
FPPGWVEWNDKFRDNVRAFWRGDGGQLAELATRLTGSADLFDHSGRRPTASVNFVTAHDGFTLRDLVSYEGKHNLANGED
GRDGSDHNISSNYGVEGETNDPAIKQLRRQQMRNLLATLLLSQGTPMLLAGDEFGHSQNGNNNAYCQDNELTWIDWTAAT
KAAAADQAAFVRRLIRIRQRYPLLHRARFFDGKFDEALGLKDLTWLAPNGTEMDEAGWHDPEARALMLRLDGRSPTTGLR
EIAANVTLLMLINAASTAVSFTLPAMHDEHWRVLVDTARHGGRVVAGGSEWKAPAHSLTLLAVERDRIVSSARIVSEGAR
>Mature_719_residues
ATRKFTQRSRIREGRPNPLGATWDGLGVNFALYSRNATRVELCLFDERGREQERIALPEYTDEVWHGYLPDARPGQLYGY
RVHGPYAPDAGHRFNHNKLLLDPYAKQIVGELKWAPHLFGYTIGHRDKDLSFDRRDSAAFMPKSAVIDPAFTWGQDRPPQ
TPWNRTVIYEAHVRGLSMLHPAVPPEERGTFSALKTDELIDHISSLGVTAVELLPVHAFVDDQYLLEKGLRNYWGYNTLG
FFAPQARYMSTRTVAEFKQMVARLHHAGLEVLLDVVYNHTAEGNELGPTLSFKGIDNASYYRLADDRRFYINDTGTGNTF
DLTNVGALRMVMDSLRYWVQEMHVDGFRFDLASILGRERYGFDPSGSFLDAVRQDPVLSQTKLIAEPWDIGPGGYQVGNF
PPGWVEWNDKFRDNVRAFWRGDGGQLAELATRLTGSADLFDHSGRRPTASVNFVTAHDGFTLRDLVSYEGKHNLANGEDG
RDGSDHNISSNYGVEGETNDPAIKQLRRQQMRNLLATLLLSQGTPMLLAGDEFGHSQNGNNNAYCQDNELTWIDWTAATK
AAAADQAAFVRRLIRIRQRYPLLHRARFFDGKFDEALGLKDLTWLAPNGTEMDEAGWHDPEARALMLRLDGRSPTTGLRE
IAANVTLLMLINAASTAVSFTLPAMHDEHWRVLVDTARHGGRVVAGGSEWKAPAHSLTLLAVERDRIVSSARIVSEGAR

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=185, Percent_Identity=27.5675675675676, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI2367229, Length=694, Percent_Identity=46.3976945244957, Blast_Score=595, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI17554896, Length=196, Percent_Identity=30.1020408163265, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI32564391, Length=196, Percent_Identity=30.1020408163265, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320826, Length=230, Percent_Identity=27.8260869565217, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 80895; Mature: 80763

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATRKFTQRSRIREGRPNPLGATWDGLGVNFALYSRNATRVELCLFDERGREQERIALPE
CCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCEECCC
YTDEVWHGYLPDARPGQLYGYRVHGPYAPDAGHRFNHNKLLLDPYAKQIVGELKWAPHLF
HHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCHHH
GYTIGHRDKDLSFDRRDSAAFMPKSAVIDPAFTWGQDRPPQTPWNRTVIYEAHVRGLSML
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHCCHHHH
HPAVPPEERGTFSALKTDELIDHISSLGVTAVELLPVHAFVDDQYLLEKGLRNYWGYNTL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEHEEEEHHHCCHHHHHHHHHHHHCCCCCH
GFFAPQARYMSTRTVAEFKQMVARLHHAGLEVLLDVVYNHTAEGNELGPTLSFKGIDNAS
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCC
YYRLADDRRFYINDTGTGNTFDLTNVGALRMVMDSLRYWVQEMHVDGFRFDLASILGRER
EEEEECCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHH
YGFDPSGSFLDAVRQDPVLSQTKLIAEPWDIGPGGYQVGNFPPGWVEWNDKFRDNVRAFW
CCCCCCCHHHHHHHCCCCHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCHHHHHCCEEEE
RGDGGQLAELATRLTGSADLFDHSGRRPTASVNFVTAHDGFTLRDLVSYEGKHNLANGED
ECCCCHHHHHHHHHCCCCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCCCCC
GRDGSDHNISSNYGVEGETNDPAIKQLRRQQMRNLLATLLLSQGTPMLLAGDEFGHSQNG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
NNNAYCQDNELTWIDWTAATKAAAADQAAFVRRLIRIRQRYPLLHRARFFDGKFDEALGL
CCCCEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHCCC
KDLTWLAPNGTEMDEAGWHDPEARALMLRLDGRSPTTGLREIAANVTLLMLINAASTAVS
CCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCEEEEEEEECCHHEEE
FTLPAMHDEHWRVLVDTARHGGRVVAGGSEWKAPAHSLTLLAVERDRIVSSARIVSEGAR
EEECCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCEEEEEEEEHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ATRKFTQRSRIREGRPNPLGATWDGLGVNFALYSRNATRVELCLFDERGREQERIALPE
CCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCEECCC
YTDEVWHGYLPDARPGQLYGYRVHGPYAPDAGHRFNHNKLLLDPYAKQIVGELKWAPHLF
HHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECHHHHHHHHHHCCCCHHH
GYTIGHRDKDLSFDRRDSAAFMPKSAVIDPAFTWGQDRPPQTPWNRTVIYEAHVRGLSML
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHCCHHHH
HPAVPPEERGTFSALKTDELIDHISSLGVTAVELLPVHAFVDDQYLLEKGLRNYWGYNTL
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEHEEEEHHHCCHHHHHHHHHHHHCCCCCH
GFFAPQARYMSTRTVAEFKQMVARLHHAGLEVLLDVVYNHTAEGNELGPTLSFKGIDNAS
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCC
YYRLADDRRFYINDTGTGNTFDLTNVGALRMVMDSLRYWVQEMHVDGFRFDLASILGRER
EEEEECCCEEEEECCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHH
YGFDPSGSFLDAVRQDPVLSQTKLIAEPWDIGPGGYQVGNFPPGWVEWNDKFRDNVRAFW
CCCCCCCHHHHHHHCCCCHHHHHHCCCCCCCCCCCEECCCCCCCCCCCCHHHHHCCEEEE
RGDGGQLAELATRLTGSADLFDHSGRRPTASVNFVTAHDGFTLRDLVSYEGKHNLANGED
ECCCCHHHHHHHHHCCCCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHCCCCCCCCCCCC
GRDGSDHNISSNYGVEGETNDPAIKQLRRQQMRNLLATLLLSQGTPMLLAGDEFGHSQNG
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCC
NNNAYCQDNELTWIDWTAATKAAAADQAAFVRRLIRIRQRYPLLHRARFFDGKFDEALGL
CCCCEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHHHCCC
KDLTWLAPNGTEMDEAGWHDPEARALMLRLDGRSPTTGLREIAANVTLLMLINAASTAVS
CCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCEEEEEEEECCHHEEE
FTLPAMHDEHWRVLVDTARHGGRVVAGGSEWKAPAHSLTLLAVERDRIVSSARIVSEGAR
EEECCCCCCCCEEEEEECCCCCEEEECCCCCCCCCCEEEEEEEEHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]