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The map label for this gene is yfjR [H]

Identifier: 21241096

GI number: 21241096

Start: 383622

End: 384500

Strand: Direct

Name: yfjR [H]

Synonym: XAC0322

Alternate gene names: 21241096

Gene position: 383622-384500 (Clockwise)

Preceding gene: 21241095

Following gene: 21241097

Centisome position: 7.41

GC content: 68.26

Gene sequence:

>879_bases
ATGCCGATAGGATTTCTGGGCTTGGGCACGATGGGCCTGCCGATGGCGCACAACCTGCTGCGTGGCGGGTTCGCGTTGAG
CGTGTGGAACCGGTCGCCCGAACGCGCTCACTCGCTGCGCGATGCGGGCGCCACGGTGGTGGAGCAACCGCGCGATGCCG
CGCACGGCCCGCTGCTGTTTTCGATGCTGGCCGACGACAACGCAGTGCGCCAGACCTTGCTGGAGCGTGGCGTGCTGGAT
GCGCTGCCGGCGGGCAGCGTGCACGTCAACATGGCCACCATTTCGGTGGCGCTGGCACATGAGTTGACCGCCCTGCATGC
CGAGCGCGGTGTGGCCTACGTGGCGGCGCCGGTGCTTGGGCGTGTGGAGGTGGCCGAAGCCGGCAATCTCAATATTCTGG
CCGCCGGCGATGAAGCGGCCGTGGCCAGGGTGCAGCCGATGTTCGATGCGCTGGGGCAGAAGACCTGGTACATCGGGCGC
GCACCGGAGCAGGCCAATGCAGTGAAGCTGGCTGCCAACTTCTGCCTGGCCAGCGCCATCGGCGCCATGGCCGAAGCCAG
TGCGCTGGCACGCGGTCATGGCGTGGATGCGACGCAGTTTCTGGGCATGCTCACCAGCACCCTGTTCGCCGCGCCCGCCT
ACCAGGGCTACGGCCGCCTGATCATGCAGCGCGAATACCTGCCGGCTGGTTTCACCGCCACGCTGGGCCGCAAGGACGTG
GACCTGGCCATCCAGGCTGGCGCCGACAAGCAGGTGCCGATGCCATTGGGCGAATTGCTGCGTGCAGGGCTGGACGAGGC
CATTGCGCATGGCGACGGCCACGCAGACTGGGCGGTACTGGCGGAAGTCTCGGCGCGGCGCGCAGGGCAGGTAGCCTGA

Upstream 100 bases:

>100_bases
TCACTCCGGCACCGCAATACCTGCGTTGCGAACCGCGCGATGCGCGGGGCGTGTTCGCCAACTGATTTGCACCTGACGTC
ATACCGAACAAGGACACCGC

Downstream 100 bases:

>100_bases
TCACCATTGCGGAACTCGGATTGAGTCCTGCGCAAGGCACTTCGCTTCACGCCAAGGCGGCCCACCGCTTGCTCACCTAA
ACATGCAGCAGACCTTCTAA

Product: dehydrogenase

Products: (R)-Glycerate; Nicotinamide adenine dinucleotide [C]

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MPIGFLGLGTMGLPMAHNLLRGGFALSVWNRSPERAHSLRDAGATVVEQPRDAAHGPLLFSMLADDNAVRQTLLERGVLD
ALPAGSVHVNMATISVALAHELTALHAERGVAYVAAPVLGRVEVAEAGNLNILAAGDEAAVARVQPMFDALGQKTWYIGR
APEQANAVKLAANFCLASAIGAMAEASALARGHGVDATQFLGMLTSTLFAAPAYQGYGRLIMQREYLPAGFTATLGRKDV
DLAIQAGADKQVPMPLGELLRAGLDEAIAHGDGHADWAVLAEVSARRAGQVA

Sequences:

>Translated_292_residues
MPIGFLGLGTMGLPMAHNLLRGGFALSVWNRSPERAHSLRDAGATVVEQPRDAAHGPLLFSMLADDNAVRQTLLERGVLD
ALPAGSVHVNMATISVALAHELTALHAERGVAYVAAPVLGRVEVAEAGNLNILAAGDEAAVARVQPMFDALGQKTWYIGR
APEQANAVKLAANFCLASAIGAMAEASALARGHGVDATQFLGMLTSTLFAAPAYQGYGRLIMQREYLPAGFTATLGRKDV
DLAIQAGADKQVPMPLGELLRAGLDEAIAHGDGHADWAVLAEVSARRAGQVA
>Mature_291_residues
PIGFLGLGTMGLPMAHNLLRGGFALSVWNRSPERAHSLRDAGATVVEQPRDAAHGPLLFSMLADDNAVRQTLLERGVLDA
LPAGSVHVNMATISVALAHELTALHAERGVAYVAAPVLGRVEVAEAGNLNILAAGDEAAVARVQPMFDALGQKTWYIGRA
PEQANAVKLAANFCLASAIGAMAEASALARGHGVDATQFLGMLTSTLFAAPAYQGYGRLIMQREYLPAGFTATLGRKDVD
LAIQAGADKQVPMPLGELLRAGLDEAIAHGDGHADWAVLAEVSARRAGQVA

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=285, Percent_Identity=29.8245614035088, Blast_Score=111, Evalue=7e-25,
Organism=Homo sapiens, GI40556376, Length=258, Percent_Identity=30.6201550387597, Blast_Score=105, Evalue=4e-23,
Organism=Escherichia coli, GI1786719, Length=284, Percent_Identity=30.6338028169014, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI145693186, Length=283, Percent_Identity=28.2685512367491, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790315, Length=295, Percent_Identity=31.1864406779661, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17557316, Length=265, Percent_Identity=26.4150943396226, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24655230, Length=296, Percent_Identity=27.7027027027027, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922568, Length=296, Percent_Identity=27.7027027027027, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28574115, Length=264, Percent_Identity=24.2424242424242, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.60 [C]

Molecular weight: Translated: 30453; Mature: 30322

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIGFLGLGTMGLPMAHNLLRGGFALSVWNRSPERAHSLRDAGATVVEQPRDAAHGPLLF
CCEEEEECCCCCHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCHHHHCCCCCCCCCEEE
SMLADDNAVRQTLLERGVLDALPAGSVHVNMATISVALAHELTALHAERGVAYVAAPVLG
HHHHCCHHHHHHHHHCCHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEHHHHC
RVEVAEAGNLNILAAGDEAAVARVQPMFDALGQKTWYIGRAPEQANAVKLAANFCLASAI
CEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHH
GAMAEASALARGHGVDATQFLGMLTSTLFAAPAYQGYGRLIMQREYLPAGFTATLGRKDV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHCHHHHEEECCCCCCCCEECCCCCCC
DLAIQAGADKQVPMPLGELLRAGLDEAIAHGDGHADWAVLAEVSARRAGQVA
EEEEECCCCCCCCCCHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PIGFLGLGTMGLPMAHNLLRGGFALSVWNRSPERAHSLRDAGATVVEQPRDAAHGPLLF
CEEEEECCCCCHHHHHHHHHCCEEEEEECCCHHHHHHHHHCCCHHHHCCCCCCCCCEEE
SMLADDNAVRQTLLERGVLDALPAGSVHVNMATISVALAHELTALHAERGVAYVAAPVLG
HHHHCCHHHHHHHHHCCHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEEEHHHHC
RVEVAEAGNLNILAAGDEAAVARVQPMFDALGQKTWYIGRAPEQANAVKLAANFCLASAI
CEEEECCCCEEEEECCCHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHH
GAMAEASALARGHGVDATQFLGMLTSTLFAAPAYQGYGRLIMQREYLPAGFTATLGRKDV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHCHHHHEEECCCCCCCCEECCCCCCC
DLAIQAGADKQVPMPLGELLRAGLDEAIAHGDGHADWAVLAEVSARRAGQVA
EEEEECCCCCCCCCCHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Hydroxy-3-oxopropanoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: 2-Hydroxy-3-oxopropanoate + H+ + Nicotinamide adenine dinucleotide - reduced --> (R)-Glycerate + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]