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The map label for this gene is rihA [H]

Identifier: 21241081

GI number: 21241081

Start: 366399

End: 367568

Strand: Reverse

Name: rihA [H]

Synonym: XAC0307

Alternate gene names: 21241081

Gene position: 367568-366399 (Counterclockwise)

Preceding gene: 77748521

Following gene: 21241080

Centisome position: 7.1

GC content: 65.47

Gene sequence:

>1170_bases
ATGCCTCACTCCATCGCCGGTGCAATGCCTTCAACCTTCCACGACAAAAACGCAATGACCGACCCGCACCTCCCCTCATC
CAGACGCCGCGTCATTCTCGAAGACGACATCGATGGCTTCACCCCCGCACAGCTGCTGCTGTTGCAGTCGCCCGACGTCG
AGGTGCTGGGTATCAGTGCCGTCAGCGGCAATATCTGGCGCGATGAGGTGCTGGCGCATACCTGCCGCCTGCTGGAACTG
GCTGGCCATCCGCAGATTCCGGTGCTTCCCGGCCCGGTGCATCCGCTGATCAACAGCGAGTTGGCCACCGAACGCTGGGA
AGCGCTGTACGGCAAACTCGTGTGGAAAGGCGCATGGACCAAGCACTGGGTGGACGGCGACACCGTGCAAAGCGCGCCGC
GCTATCACGCCCACGATGTGGTGCCCGATCTGGCATTGGGCAATCCCAGTGTGGTGCGTCCCTCAGACGAGCCGGCCGCG
TTGTTCATGCTGCGCATGGTGCGTCAGTACCCGGGCGAGGTCAGCATCATTGCCACTGGCCCGTTGACCAATCTGGCACT
GGCGCAATCGCTGGACCCGGCCTTTGCCACGCTGGCGCGCGAGCTGGTCTATATGGGTGGCAGCCTCAATCCGCGGCAGC
AACGCAACAGCGTGAGTGCGCAGCAATTCGCACGCGAATTCATCAATTCGCCGCGACGCGAGTTCAATATCCGCTGGGAC
CCCGAAGCGGCCAGCATCGTCATGCGCGCGCCGTGGCGCCGGATCACCATGGTGCCGGTCGACCCGTCCACCGCCACCGA
ACTCACCGCCGAGTTGCTGGCGCGCATGAGTGCGGCCGACACCACGATTGGCCACGCACTGCGCCGCCGCGAGCCGGGCT
TTCCGATGTGGGACGAACTGGCCACGGCCGTGTGGCTGCGCCCGGAATTGGCGAGGCATACGGAAACGCTGTACGTCGAC
ACCAACACTGCCTTCGATGCCGGGTACGGCGATATCTTGTCGTGGGCGCCGGGGTATCAGCCCGGGCTGGGCGAGCAGGC
GCAGACGGTGGTGCATGCGGTGGATGTGCCGGCATTGGAGCAGCTGCTGGTCGACCGCCTCACCGCGCCACAGCCGCCGG
CAGTGGGATCGCCCCTCCCCCTTCGCCCATCGGGACCTCGGTCCCCTTGA

Upstream 100 bases:

>100_bases
TAGCGCCGACAACGACACGACATATTCCACGTAGGAGCGCGCCCGAGCGCGAACGGACTTTACCGGTAACGCCCCGTCGC
GCGCAGGCACGCTCCTACAG

Downstream 100 bases:

>100_bases
TGGGGAGAGGGGACCGCAGGGCGGATGAGGGTGCGATAGCCGGTCGCCCTGCGACGCCGGTTGAGTAGGCGGCACTTTAC
GTGGAAGCAAATGCGTGACA

Product: nucleoside hydrolase

Products: cytosine; ribose; uracil [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MPHSIAGAMPSTFHDKNAMTDPHLPSSRRRVILEDDIDGFTPAQLLLLQSPDVEVLGISAVSGNIWRDEVLAHTCRLLEL
AGHPQIPVLPGPVHPLINSELATERWEALYGKLVWKGAWTKHWVDGDTVQSAPRYHAHDVVPDLALGNPSVVRPSDEPAA
LFMLRMVRQYPGEVSIIATGPLTNLALAQSLDPAFATLARELVYMGGSLNPRQQRNSVSAQQFAREFINSPRREFNIRWD
PEAASIVMRAPWRRITMVPVDPSTATELTAELLARMSAADTTIGHALRRREPGFPMWDELATAVWLRPELARHTETLYVD
TNTAFDAGYGDILSWAPGYQPGLGEQAQTVVHAVDVPALEQLLVDRLTAPQPPAVGSPLPLRPSGPRSP

Sequences:

>Translated_389_residues
MPHSIAGAMPSTFHDKNAMTDPHLPSSRRRVILEDDIDGFTPAQLLLLQSPDVEVLGISAVSGNIWRDEVLAHTCRLLEL
AGHPQIPVLPGPVHPLINSELATERWEALYGKLVWKGAWTKHWVDGDTVQSAPRYHAHDVVPDLALGNPSVVRPSDEPAA
LFMLRMVRQYPGEVSIIATGPLTNLALAQSLDPAFATLARELVYMGGSLNPRQQRNSVSAQQFAREFINSPRREFNIRWD
PEAASIVMRAPWRRITMVPVDPSTATELTAELLARMSAADTTIGHALRRREPGFPMWDELATAVWLRPELARHTETLYVD
TNTAFDAGYGDILSWAPGYQPGLGEQAQTVVHAVDVPALEQLLVDRLTAPQPPAVGSPLPLRPSGPRSP
>Mature_388_residues
PHSIAGAMPSTFHDKNAMTDPHLPSSRRRVILEDDIDGFTPAQLLLLQSPDVEVLGISAVSGNIWRDEVLAHTCRLLELA
GHPQIPVLPGPVHPLINSELATERWEALYGKLVWKGAWTKHWVDGDTVQSAPRYHAHDVVPDLALGNPSVVRPSDEPAAL
FMLRMVRQYPGEVSIIATGPLTNLALAQSLDPAFATLARELVYMGGSLNPRQQRNSVSAQQFAREFINSPRREFNIRWDP
EAASIVMRAPWRRITMVPVDPSTATELTAELLARMSAADTTIGHALRRREPGFPMWDELATAVWLRPELARHTETLYVDT
NTAFDAGYGDILSWAPGYQPGLGEQAQTVVHAVDVPALEQLLVDRLTAPQPPAVGSPLPLRPSGPRSP

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788486, Length=342, Percent_Identity=22.8070175438596, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786871, Length=341, Percent_Identity=25.5131964809384, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 42766; Mature: 42635

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHSIAGAMPSTFHDKNAMTDPHLPSSRRRVILEDDIDGFTPAQLLLLQSPDVEVLGISA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHEEEECCCCCEEEEEEE
VSGNIWRDEVLAHTCRLLELAGHPQIPVLPGPVHPLINSELATERWEALYGKLVWKGAWT
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCC
KHWVDGDTVQSAPRYHAHDVVPDLALGNPSVVRPSDEPAALFMLRMVRQYPGEVSIIATG
CCCCCCCHHHCCCCCCHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCEEEEECC
PLTNLALAQSLDPAFATLARELVYMGGSLNPRQQRNSVSAQQFAREFINSPRREFNIRWD
CCHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHCCCHHHCCCEEC
PEAASIVMRAPWRRITMVPVDPSTATELTAELLARMSAADTTIGHALRRREPGFPMWDEL
CHHHHEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ATAVWLRPELARHTETLYVDTNTAFDAGYGDILSWAPGYQPGLGEQAQTVVHAVDVPALE
HHHHHHCHHHHCCCEEEEEECCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHCHHHHH
QLLVDRLTAPQPPAVGSPLPLRPSGPRSP
HHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PHSIAGAMPSTFHDKNAMTDPHLPSSRRRVILEDDIDGFTPAQLLLLQSPDVEVLGISA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHEEEECCCCCEEEEEEE
VSGNIWRDEVLAHTCRLLELAGHPQIPVLPGPVHPLINSELATERWEALYGKLVWKGAWT
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCC
KHWVDGDTVQSAPRYHAHDVVPDLALGNPSVVRPSDEPAALFMLRMVRQYPGEVSIIATG
CCCCCCCHHHCCCCCCHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHHCCCCEEEEECC
PLTNLALAQSLDPAFATLARELVYMGGSLNPRQQRNSVSAQQFAREFINSPRREFNIRWD
CCHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHCCCHHHCCCEEC
PEAASIVMRAPWRRITMVPVDPSTATELTAELLARMSAADTTIGHALRRREPGFPMWDEL
CHHHHEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
ATAVWLRPELARHTETLYVDTNTAFDAGYGDILSWAPGYQPGLGEQAQTVVHAVDVPALE
HHHHHHCHHHHCCCEEEEEECCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHCHHHHH
QLLVDRLTAPQPPAVGSPLPLRPSGPRSP
HHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cytidine; H2O; uridine [C]

Specific reaction: cytidine + H2O = cytosine + ribose uridine + H2O = uracil + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA