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The map label for this gene is gatA [H]

Identifier: 21241080

GI number: 21241080

Start: 364456

End: 365868

Strand: Reverse

Name: gatA [H]

Synonym: XAC0306

Alternate gene names: 21241080

Gene position: 365868-364456 (Counterclockwise)

Preceding gene: 21241081

Following gene: 21241079

Centisome position: 7.07

GC content: 71.76

Gene sequence:

>1413_bases
GTGCTGCGCGGGCACGCGCAGCTGCTGGCCACCTTCCATGGGCCGCCTGCAACGGCGCATGAGGGCGGCGGCCCACGCGG
CCAGATCGCCTCCATCGTCAGCACTACGCGCAGCCAGCCCACACTGGCCGCGCGACTGGCCACCCAGACGCTGACACGCA
TCAACCAGGCCAACGACCGCCTGTGCGCCATTACCCGCCTGCTGCCTGCGCGTGCCGCGGCCGATGCCGCGCGCGTGCAG
GCTGCGCTTGCCGGTGGCAGCGACGGTGGCGCGTTGGCCGGCGTGCCATTCGTGGTCAAGGACCTGTTCGATGTGGCCGG
GCAGGTCACCACCGCCGGCGCCGCAGTGCGTGCGCATTGCCCGCCTGCCAGCCGCGATGCAGCGGTGGTGCAACGGCTCG
GCGAAGCCGGCGCAGTGTTGGTGGGTACCGCCAACATGGACGAATTCGCGTACGGCTTCGCCACGGTCAATGCGCATTAC
GGCACGACTGCCAATCCGCACGATGACCAGCACCTGGCGGGCGGCTCATCGGGCGGCTCGGCGGCAGCGGTGGCCGCCGG
GCTGGTGCCGTTCGCGCTTGGCTCGGACACCAACGGCTCCATCCGCGTGCCGGCCGCGCTGTGCGGCATCTACGGCCTGC
GCCCCACGCACGGCGCCGTGCCCCTGGATGGGGTGTTCCCATTTGTCGATGCACTGGATGTGGTCGGCCCATTCGCCACC
TCGGTGGCCGACCTGCGCTGCGTGTACGACGTACTGCGTGGGCAGCCGCTGCCTTCGTGCCATGCCGGCAAGCTGCGCGT
CGCGCGGTTGGGCGGCTGGTTCCAGCGCAATCTGGATCCGGATCTGGAGGCCGGGCTGCAGGCCCTGCTCGCCGCCTGCA
ACAGCCTTGCGTCGATCGACCTGCCCGAGGCCGAACGCGCACGCGCCGCGGCCTTCGTCCTGACCGCTGCCGAAGGCGGG
CATCGTCACCGCAGCGCGCTCACCGCGCACGGCGCGCAGTTCGACCCGGCCACCCGCGACCGCCTGCTTGCCGGCTTGCA
GCTGCCTGCCAGCGCGGTGGCCGATGCGCACCGCTTCGCGCAGTGGTTTGCCGATGCCATGCACGCGCTGTGGGACCAGG
TGGATGTGCTCATCGCGCCGGCCACTCCCTGCGTGGCGCCACGCATCGACCAGGACACCATCCAGATCGACGGTTTGCCC
GTCTCCGCGCGTGCGAATCTGGGTATTTTCACCCAGCCACTCGGCCTGGCCGCCTGCCCGGTGCTGGCCGCACCGTTGCC
GCGTCCCGGGCGCCTGCCACTGGGCGTGCAACTGATCGCCGCGCCCGGCCGGGAAGACCGCCTGTTTGCGCTGGCCGCGC
AACTCGAGCGCGACGGCCTGCTCGCCTTCAGCCCGCCGCCGGAGCCACGCTGA

Upstream 100 bases:

>100_bases
CTCGAATCACAAATCCCCAATCCCCAAATCCCCGCTCCCCAACAGGTGATCCATGCAACTCCCAGCGCAGTGGCAGGCCG
GCGTCGATCGCAATACCGAA

Downstream 100 bases:

>100_bases
TGCTGCAGACCCTGCGCGCGCGCCGCGGCATGGTGGTCGCACCGCATCATCTTGCCGCACAGGCTGGACGCGATGTCTTG
CGCGACGGTGGCACTGCCAT

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MLRGHAQLLATFHGPPATAHEGGGPRGQIASIVSTTRSQPTLAARLATQTLTRINQANDRLCAITRLLPARAAADAARVQ
AALAGGSDGGALAGVPFVVKDLFDVAGQVTTAGAAVRAHCPPASRDAAVVQRLGEAGAVLVGTANMDEFAYGFATVNAHY
GTTANPHDDQHLAGGSSGGSAAAVAAGLVPFALGSDTNGSIRVPAALCGIYGLRPTHGAVPLDGVFPFVDALDVVGPFAT
SVADLRCVYDVLRGQPLPSCHAGKLRVARLGGWFQRNLDPDLEAGLQALLAACNSLASIDLPEAERARAAAFVLTAAEGG
HRHRSALTAHGAQFDPATRDRLLAGLQLPASAVADAHRFAQWFADAMHALWDQVDVLIAPATPCVAPRIDQDTIQIDGLP
VSARANLGIFTQPLGLAACPVLAAPLPRPGRLPLGVQLIAAPGREDRLFALAAQLERDGLLAFSPPPEPR

Sequences:

>Translated_470_residues
MLRGHAQLLATFHGPPATAHEGGGPRGQIASIVSTTRSQPTLAARLATQTLTRINQANDRLCAITRLLPARAAADAARVQ
AALAGGSDGGALAGVPFVVKDLFDVAGQVTTAGAAVRAHCPPASRDAAVVQRLGEAGAVLVGTANMDEFAYGFATVNAHY
GTTANPHDDQHLAGGSSGGSAAAVAAGLVPFALGSDTNGSIRVPAALCGIYGLRPTHGAVPLDGVFPFVDALDVVGPFAT
SVADLRCVYDVLRGQPLPSCHAGKLRVARLGGWFQRNLDPDLEAGLQALLAACNSLASIDLPEAERARAAAFVLTAAEGG
HRHRSALTAHGAQFDPATRDRLLAGLQLPASAVADAHRFAQWFADAMHALWDQVDVLIAPATPCVAPRIDQDTIQIDGLP
VSARANLGIFTQPLGLAACPVLAAPLPRPGRLPLGVQLIAAPGREDRLFALAAQLERDGLLAFSPPPEPR
>Mature_470_residues
MLRGHAQLLATFHGPPATAHEGGGPRGQIASIVSTTRSQPTLAARLATQTLTRINQANDRLCAITRLLPARAAADAARVQ
AALAGGSDGGALAGVPFVVKDLFDVAGQVTTAGAAVRAHCPPASRDAAVVQRLGEAGAVLVGTANMDEFAYGFATVNAHY
GTTANPHDDQHLAGGSSGGSAAAVAAGLVPFALGSDTNGSIRVPAALCGIYGLRPTHGAVPLDGVFPFVDALDVVGPFAT
SVADLRCVYDVLRGQPLPSCHAGKLRVARLGGWFQRNLDPDLEAGLQALLAACNSLASIDLPEAERARAAAFVLTAAEGG
HRHRSALTAHGAQFDPATRDRLLAGLQLPASAVADAHRFAQWFADAMHALWDQVDVLIAPATPCVAPRIDQDTIQIDGLP
VSARANLGIFTQPLGLAACPVLAAPLPRPGRLPLGVQLIAAPGREDRLFALAAQLERDGLLAFSPPPEPR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=485, Percent_Identity=26.3917525773196, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI222831590, Length=455, Percent_Identity=27.4725274725275, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI166795287, Length=149, Percent_Identity=35.5704697986577, Blast_Score=66, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=396, Percent_Identity=27.7777777777778, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17537465, Length=475, Percent_Identity=26.7368421052632, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17556264, Length=181, Percent_Identity=34.2541436464088, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=450, Percent_Identity=25.3333333333333, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71990152, Length=155, Percent_Identity=34.8387096774194, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=162, Percent_Identity=37.037037037037, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17556278, Length=162, Percent_Identity=37.037037037037, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=382, Percent_Identity=31.413612565445, Blast_Score=117, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6323950, Length=393, Percent_Identity=24.9363867684478, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320448, Length=169, Percent_Identity=31.3609467455621, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648113, Length=473, Percent_Identity=32.3467230443975, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21356731, Length=411, Percent_Identity=28.2238442822384, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24652985, Length=479, Percent_Identity=24.0083507306889, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19922090, Length=479, Percent_Identity=24.0083507306889, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24652981, Length=479, Percent_Identity=24.0083507306889, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24652983, Length=479, Percent_Identity=24.0083507306889, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24644968, Length=245, Percent_Identity=29.7959183673469, Blast_Score=96, Evalue=6e-20,
Organism=Drosophila melanogaster, GI45550774, Length=252, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648435, Length=252, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648437, Length=252, Percent_Identity=28.5714285714286, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI161078093, Length=481, Percent_Identity=23.9085239085239, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24648441, Length=406, Percent_Identity=24.1379310344828, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648439, Length=406, Percent_Identity=24.1379310344828, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 48332; Mature: 48332

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRGHAQLLATFHGPPATAHEGGGPRGQIASIVSTTRSQPTLAARLATQTLTRINQANDR
CCCCCEEHEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCH
LCAITRLLPARAAADAARVQAALAGGSDGGALAGVPFVVKDLFDVAGQVTTAGAAVRAHC
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECC
PPASRDAAVVQRLGEAGAVLVGTANMDEFAYGFATVNAHYGTTANPHDDQHLAGGSSGGS
CCCCCHHHHHHHHCCCCEEEEECCCCHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCC
AAAVAAGLVPFALGSDTNGSIRVPAALCGIYGLRPTHGAVPLDGVFPFVDALDVVGPFAT
HHHHHHHHHHHEECCCCCCCEECHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
SVADLRCVYDVLRGQPLPSCHAGKLRVARLGGWFQRNLDPDLEAGLQALLAACNSLASID
HHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHCCC
LPEAERARAAAFVLTAAEGGHRHRSALTAHGAQFDPATRDRLLAGLQLPASAVADAHRFA
CCCHHHHHEEEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
QWFADAMHALWDQVDVLIAPATPCVAPRIDQDTIQIDGLPVSARANLGIFTQPLGLAACP
HHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEECCEEEECCCCCCEEECCCHHHHHH
VLAAPLPRPGRLPLGVQLIAAPGREDRLFALAAQLERDGLLAFSPPPEPR
HHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEECCCCCCC
>Mature Secondary Structure
MLRGHAQLLATFHGPPATAHEGGGPRGQIASIVSTTRSQPTLAARLATQTLTRINQANDR
CCCCCEEHEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCH
LCAITRLLPARAAADAARVQAALAGGSDGGALAGVPFVVKDLFDVAGQVTTAGAAVRAHC
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECC
PPASRDAAVVQRLGEAGAVLVGTANMDEFAYGFATVNAHYGTTANPHDDQHLAGGSSGGS
CCCCCHHHHHHHHCCCCEEEEECCCCHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCCC
AAAVAAGLVPFALGSDTNGSIRVPAALCGIYGLRPTHGAVPLDGVFPFVDALDVVGPFAT
HHHHHHHHHHHEECCCCCCCEECHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
SVADLRCVYDVLRGQPLPSCHAGKLRVARLGGWFQRNLDPDLEAGLQALLAACNSLASID
HHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHCCCCCCHHHHHHHHHHHHHHHHCCC
LPEAERARAAAFVLTAAEGGHRHRSALTAHGAQFDPATRDRLLAGLQLPASAVADAHRFA
CCCHHHHHEEEEEEEECCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHH
QWFADAMHALWDQVDVLIAPATPCVAPRIDQDTIQIDGLPVSARANLGIFTQPLGLAACP
HHHHHHHHHHHHCCEEEEECCCCCCCCCCCCCEEEECCEEEECCCCCCEEECCCHHHHHH
VLAAPLPRPGRLPLGVQLIAAPGREDRLFALAAQLERDGLLAFSPPPEPR
HHHCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA