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The map label for this gene is dnaN [H]

Identifier: 21240776

GI number: 21240776

Start: 1647

End: 2747

Strand: Direct

Name: dnaN [H]

Synonym: XAC0002

Alternate gene names: 21240776

Gene position: 1647-2747 (Clockwise)

Preceding gene: 21240775

Following gene: 21240777

Centisome position: 0.03

GC content: 62.49

Gene sequence:

>1101_bases
ATGCGTTTTACACTGCAGCGCGAAGCCTTCCTCAAACCATTGGCACAAGTGGTCAATGTGGTCGAACGCCGTCAAACCCT
GCCCGTCCTGGCCAACTTGCTGGTACAGGTCAAGGATGGACAGGTCTCGTTGACCGGCACCGACCTGGAAGTGGAAATGA
TCTCGCGCACGCTGGTGGAGGACGCGCAGGACGGCGAAACCACGATTCCGGCGCGCAAGCTGTTCGACATCCTGCGCGCC
CTGCCCGACGGCAGCCGCGTCACCATTTCGCAGACCGGCGACAAGGTCACTGTGCAAGCCGGGCGGAGCCGCTTCACCCT
GGCGACGTTGCCTTCCAACGATTTTCCGTCTGTGGACGAAGTCGAAGCGACCGAACGCGTCGTGGTGCCCGAAGCCGGGC
TCAAGGAGCTGATCGAGCGCACCGCGTTCGCCATGGCCCAGCAGGACGTGCGCTATTACCTCAATGGCCTGTTGTTCGAC
CTGCGCGACGGCTTGCTGCGCTGCGTGGCTACCGACGGCCATCGTCTGGCGCTATGTGAGATGGAGCTGGAGAAAGCCGG
TGGCGCCAAGCGCCAGATCATCGTGCCGCGCAAGGGCGTGACCGAGCTGCAGCGTTTGCTGGAAGGCGCCGATCGCGAGG
TCGAGCTGGAAGTGGGCCGCAGCCATATCCGCGTCAAGCGCGGTGATGTGACCTTTACCTCGAAGCTGATCGACGGGCGG
TTCCCGGACTACGAGGCAGTGATTCCGATCGGCGCCGATCGCGAGGTCAAGGTGGATCGTGAAGCGCTGCGCGCGTCGCT
ACAGCGTGCGGCCATCCTGTCCAACGAGAAGTACCGCGGTGTCCGTGTGGAAGTGTCGCCGGGTCAGCTGAAGATCAGCG
CGCATAACCCCGAGCAGGAGGAGGCGCAGGAAGAGATCGAGGCCGATACCAAGGTCGACGATCTGGCCATTGGCTTCAAC
GTCAACTATTTGCTCGACGCGCTGTCGGCATTGCGCGATGAGCACGTGGTGATCCAGCTGCGTGATGCCAATTCGTCTGC
ATTGGTGCGTGAGGCCAGCAGCGAGAAGTCGCGTCATGTGGTGATGCCGTTGCGTCTCTGA

Upstream 100 bases:

>100_bases
ACAAACAGTTATGGTGGTTATCCGCTGAAAACGGCCCTACCATCACCACCAAGCTTTTGATTTATTCCATATCTTTTAAA
GCATAGGGGCACGGAACCAC

Downstream 100 bases:

>100_bases
TATCTCGTTCCACGTGGAACACGGAAGCCCGGCAGATGCCGGGCTTTTTGTTTGCGCTGTTTCGTAGCTTGCTATGTGCA
GGGAGCTGTTGCGGCCAGGT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MRFTLQREAFLKPLAQVVNVVERRQTLPVLANLLVQVKDGQVSLTGTDLEVEMISRTLVEDAQDGETTIPARKLFDILRA
LPDGSRVTISQTGDKVTVQAGRSRFTLATLPSNDFPSVDEVEATERVVVPEAGLKELIERTAFAMAQQDVRYYLNGLLFD
LRDGLLRCVATDGHRLALCEMELEKAGGAKRQIIVPRKGVTELQRLLEGADREVELEVGRSHIRVKRGDVTFTSKLIDGR
FPDYEAVIPIGADREVKVDREALRASLQRAAILSNEKYRGVRVEVSPGQLKISAHNPEQEEAQEEIEADTKVDDLAIGFN
VNYLLDALSALRDEHVVIQLRDANSSALVREASSEKSRHVVMPLRL

Sequences:

>Translated_366_residues
MRFTLQREAFLKPLAQVVNVVERRQTLPVLANLLVQVKDGQVSLTGTDLEVEMISRTLVEDAQDGETTIPARKLFDILRA
LPDGSRVTISQTGDKVTVQAGRSRFTLATLPSNDFPSVDEVEATERVVVPEAGLKELIERTAFAMAQQDVRYYLNGLLFD
LRDGLLRCVATDGHRLALCEMELEKAGGAKRQIIVPRKGVTELQRLLEGADREVELEVGRSHIRVKRGDVTFTSKLIDGR
FPDYEAVIPIGADREVKVDREALRASLQRAAILSNEKYRGVRVEVSPGQLKISAHNPEQEEAQEEIEADTKVDDLAIGFN
VNYLLDALSALRDEHVVIQLRDANSSALVREASSEKSRHVVMPLRL
>Mature_366_residues
MRFTLQREAFLKPLAQVVNVVERRQTLPVLANLLVQVKDGQVSLTGTDLEVEMISRTLVEDAQDGETTIPARKLFDILRA
LPDGSRVTISQTGDKVTVQAGRSRFTLATLPSNDFPSVDEVEATERVVVPEAGLKELIERTAFAMAQQDVRYYLNGLLFD
LRDGLLRCVATDGHRLALCEMELEKAGGAKRQIIVPRKGVTELQRLLEGADREVELEVGRSHIRVKRGDVTFTSKLIDGR
FPDYEAVIPIGADREVKVDREALRASLQRAAILSNEKYRGVRVEVSPGQLKISAHNPEQEEAQEEIEADTKVDDLAIGFN
VNYLLDALSALRDEHVVIQLRDANSSALVREASSEKSRHVVMPLRL

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=367, Percent_Identity=50.6811989100817, Blast_Score=383, Evalue=1e-108,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40753; Mature: 40753

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFTLQREAFLKPLAQVVNVVERRQTLPVLANLLVQVKDGQVSLTGTDLEVEMISRTLVE
CCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEECCCCHHHHHHHHHHH
DAQDGETTIPARKLFDILRALPDGSRVTISQTGDKVTVQAGRSRFTLATLPSNDFPSVDE
CCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCC
VEATERVVVPEAGLKELIERTAFAMAQQDVRYYLNGLLFDLRDGLLRCVATDGHRLALCE
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEE
MELEKAGGAKRQIIVPRKGVTELQRLLEGADREVELEVGRSHIRVKRGDVTFTSKLIDGR
EEHHHCCCCCEEEEECCCCHHHHHHHHCCCCCEEEEEECCCEEEEEECCEEEEHHHHCCC
FPDYEAVIPIGADREVKVDREALRASLQRAAILSNEKYRGVRVEVSPGQLKISAHNPEQE
CCCCCEEEECCCCCCEECCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCHH
EAQEEIEADTKVDDLAIGFNVNYLLDALSALRDEHVVIQLRDANSSALVREASSEKSRHV
HHHHHHHCCCCCCCEEEECCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCCEE
VMPLRL
EEEECC
>Mature Secondary Structure
MRFTLQREAFLKPLAQVVNVVERRQTLPVLANLLVQVKDGQVSLTGTDLEVEMISRTLVE
CCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEECCCCHHHHHHHHHHH
DAQDGETTIPARKLFDILRALPDGSRVTISQTGDKVTVQAGRSRFTLATLPSNDFPSVDE
CCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCC
VEATERVVVPEAGLKELIERTAFAMAQQDVRYYLNGLLFDLRDGLLRCVATDGHRLALCE
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCEEEEEE
MELEKAGGAKRQIIVPRKGVTELQRLLEGADREVELEVGRSHIRVKRGDVTFTSKLIDGR
EEHHHCCCCCEEEEECCCCHHHHHHHHCCCCCEEEEEECCCEEEEEECCEEEEHHHHCCC
FPDYEAVIPIGADREVKVDREALRASLQRAAILSNEKYRGVRVEVSPGQLKISAHNPEQE
CCCCCEEEECCCCCCEECCHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCHH
EAQEEIEADTKVDDLAIGFNVNYLLDALSALRDEHVVIQLRDANSSALVREASSEKSRHV
HHHHHHHCCCCCCCEEEECCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHCCCCCCEE
VMPLRL
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]