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The map label for this gene is flgJ [H]

Identifier: 161485714

GI number: 161485714

Start: 2310423

End: 2311601

Strand: Reverse

Name: flgJ [H]

Synonym: XAC1978

Alternate gene names: 161485714

Gene position: 2311601-2310423 (Counterclockwise)

Preceding gene: 21242723

Following gene: 21242721

Centisome position: 44.66

GC content: 67.09

Gene sequence:

>1179_bases
ATGCGTATCGCAGCCTCGCCCATTGATCTCAACCCGAGCACCAAGGCCGATCCCGCAAAGATCGACAAGGTCTCCCGTCA
GCTCGAAGGTCAGTTCGCGCAGATGCTGGTCAAGAGCATGCGCGATGCGAGCTCCGGCGACCCGATGTTTCCGGGCCAGA
ACCAGATGTTCCGCGAAATGTACGACCAGCAGATGGCCAAGGCGTTGACCGACGGCAAGGGGCTGGGTCTGTCGGCGATG
ATTTCCAAGCAGCTCAGTGGCGACACGGGCGGCCCGGCCTTGAACACTTCGTTGAACACCGCTGAAGCGGCCAAGGCGTA
CGCCCTGGTCGCCGGCAAGCGCGATGCCTCGTTGCCGCTGCCCGCCCGCGATGGTGCGGCCACCGGCACCACGACCAGCA
GCGTGGCGAAGGCGGCCCTGGGCGCAGGCAACCTGAGCGGCATCGGCATGAGCCAGGTGCTGGATCTGATTGCCGGGCGT
ACCGGCGCTGGCGAAGCCGGCAGCGACGATGCTGCCGCGCTGAGCTGGCCGTCGGCCAACGATCGCTGGGCCGACGTAGC
CGCCAGCGATGCGGCCGATGCCAATGCCGCGGTGAATGCGAGTGCCGCCAGCAGCGCCGCTGCCAGCCTGGGCGAGCGCA
CGCCGGAAGGCTTTGTCGCCAAGATCTGGACGCATGCGCAAAAAGCCGCGCGCGAACTGGGGGTGGACCCGCGCGCGCTG
GTGGCACAGGCCGCATTGGAAACCGGCTGGGGCCGTCGCGGCATCGGCAACGGTGGCGACTCCAACAACCTGTTCGGCAT
CAAGGCCACCGGCTGGAGCGGCGACAAGGTCACCACCGGCACCCACGAATACGTCAACGGCGTCAAGACCACCGAAACCG
CCGATTTCCGCGCCTATGGCTCGGCCGAGGAGAGCTTTGCCGACTACGTGCGGCTGCTGAAGAACAACAGCCGTTACCAG
ACCGCGCTGCAGGCCGGCACCGATATCAAGGGTTTTGCACGCGGCTTGCAACAAGCCGGGTACGCCACCGATCCGGGCTA
TGCGGCCAAGATCGCGGCGATCGCCAACGGCCCGACCATCGACCGTGCGGTGGCCGCCATCGGCAACGCGGCGGCCGATC
TGTCCAACCGCTATGCCAGTACCGCCGAGCCGGCCGGACTCGGCACCATCCGTCGCTGA

Upstream 100 bases:

>100_bases
GCAGTCAACGAAGTGGGCGCCGCGCCCGGCGACCTGGTGGCCATCCTGGAAGCGCTCAAGCAAGCCGGTGCGCTCACCGC
GGAGCTAGAGGTGATCTGAC

Downstream 100 bases:

>100_bases
GGTAAACGCTCATGTCCATCATGTCCACCGGGACCAGCGCGCTCATCGCCTTCCAACGGGCGTTGTCGACCGTTAGCCAT
AACGTCGCCAACATCAATAC

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MRIAASPIDLNPSTKADPAKIDKVSRQLEGQFAQMLVKSMRDASSGDPMFPGQNQMFREMYDQQMAKALTDGKGLGLSAM
ISKQLSGDTGGPALNTSLNTAEAAKAYALVAGKRDASLPLPARDGAATGTTTSSVAKAALGAGNLSGIGMSQVLDLIAGR
TGAGEAGSDDAAALSWPSANDRWADVAASDAADANAAVNASAASSAAASLGERTPEGFVAKIWTHAQKAARELGVDPRAL
VAQAALETGWGRRGIGNGGDSNNLFGIKATGWSGDKVTTGTHEYVNGVKTTETADFRAYGSAEESFADYVRLLKNNSRYQ
TALQAGTDIKGFARGLQQAGYATDPGYAAKIAAIANGPTIDRAVAAIGNAAADLSNRYASTAEPAGLGTIRR

Sequences:

>Translated_392_residues
MRIAASPIDLNPSTKADPAKIDKVSRQLEGQFAQMLVKSMRDASSGDPMFPGQNQMFREMYDQQMAKALTDGKGLGLSAM
ISKQLSGDTGGPALNTSLNTAEAAKAYALVAGKRDASLPLPARDGAATGTTTSSVAKAALGAGNLSGIGMSQVLDLIAGR
TGAGEAGSDDAAALSWPSANDRWADVAASDAADANAAVNASAASSAAASLGERTPEGFVAKIWTHAQKAARELGVDPRAL
VAQAALETGWGRRGIGNGGDSNNLFGIKATGWSGDKVTTGTHEYVNGVKTTETADFRAYGSAEESFADYVRLLKNNSRYQ
TALQAGTDIKGFARGLQQAGYATDPGYAAKIAAIANGPTIDRAVAAIGNAAADLSNRYASTAEPAGLGTIRR
>Mature_392_residues
MRIAASPIDLNPSTKADPAKIDKVSRQLEGQFAQMLVKSMRDASSGDPMFPGQNQMFREMYDQQMAKALTDGKGLGLSAM
ISKQLSGDTGGPALNTSLNTAEAAKAYALVAGKRDASLPLPARDGAATGTTTSSVAKAALGAGNLSGIGMSQVLDLIAGR
TGAGEAGSDDAAALSWPSANDRWADVAASDAADANAAVNASAASSAAASLGERTPEGFVAKIWTHAQKAARELGVDPRAL
VAQAALETGWGRRGIGNGGDSNNLFGIKATGWSGDKVTTGTHEYVNGVKTTETADFRAYGSAEESFADYVRLLKNNSRYQ
TALQAGTDIKGFARGLQQAGYATDPGYAAKIAAIANGPTIDRAVAAIGNAAADLSNRYASTAEPAGLGTIRR

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=139, Percent_Identity=46.0431654676259, Blast_Score=109, Evalue=3e-25,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR019301
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 40107; Mature: 40107

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAASPIDLNPSTKADPAKIDKVSRQLEGQFAQMLVKSMRDASSGDPMFPGQNQMFREM
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
YDQQMAKALTDGKGLGLSAMISKQLSGDTGGPALNTSLNTAEAAKAYALVAGKRDASLPL
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCC
PARDGAATGTTTSSVAKAALGAGNLSGIGMSQVLDLIAGRTGAGEAGSDDAAALSWPSAN
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEECCCCC
DRWADVAASDAADANAAVNASAASSAAASLGERTPEGFVAKIWTHAQKAARELGVDPRAL
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
VAQAALETGWGRRGIGNGGDSNNLFGIKATGWSGDKVTTGTHEYVNGVKTTETADFRAYG
HHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCHHHCC
SAEESFADYVRLLKNNSRYQTALQAGTDIKGFARGLQQAGYATDPGYAAKIAAIANGPTI
CHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHEEEEECCCCCHH
DRAVAAIGNAAADLSNRYASTAEPAGLGTIRR
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRIAASPIDLNPSTKADPAKIDKVSRQLEGQFAQMLVKSMRDASSGDPMFPGQNQMFREM
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
YDQQMAKALTDGKGLGLSAMISKQLSGDTGGPALNTSLNTAEAAKAYALVAGKRDASLPL
HHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCCCC
PARDGAATGTTTSSVAKAALGAGNLSGIGMSQVLDLIAGRTGAGEAGSDDAAALSWPSAN
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEECCCCC
DRWADVAASDAADANAAVNASAASSAAASLGERTPEGFVAKIWTHAQKAARELGVDPRAL
CCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
VAQAALETGWGRRGIGNGGDSNNLFGIKATGWSGDKVTTGTHEYVNGVKTTETADFRAYG
HHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCHHHCC
SAEESFADYVRLLKNNSRYQTALQAGTDIKGFARGLQQAGYATDPGYAAKIAAIANGPTI
CHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHEEEEECCCCCHH
DRAVAAIGNAAADLSNRYASTAEPAGLGTIRR
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]