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The map label for this gene is murE [H]

Identifier: 57238652

GI number: 57238652

Start: 1706947

End: 1708230

Strand: Direct

Name: murE [H]

Synonym: CJE1813

Alternate gene names: 57238652

Gene position: 1706947-1708230 (Clockwise)

Preceding gene: 57238651

Following gene: 57238653

Centisome position: 96.01

GC content: 32.48

Gene sequence:

>1284_bases
GTGAAATTAAAACTTGAAAATTCATTCATTACAGATAACACCTTAGAGTGTGAAAAAGAATGCTTTTTTTTGCAAACCAC
ACAAAATGCTAAATTTCACGCTCAAGCCTTAGAAAAAGGTGCAAAAATCATTGATGTAAATGAGTGTAAAAAACTTTTAA
AAATCGATGAAAAGATTCAAATCATAGGAATCACAGGAACCAATGGCAAAACCACAACCGCAGCAGCGATTTATTCTATC
TTATTGGATTTAGGTTATAAGTGTGGGCTTTGTGGGACAAGAGGAGCTTTTATCAACGATGAACAAATCGATGAAAAATC
CTTAACCACCTCCCCTATTCTTAAAACCTTAGAATACCTACAAATAGCCACTCAAAAAAAATGTGATTTTTTCATCATGG
AAGTAAGCTCACACGCCTTAATTCAAAACCGCATCGAAGGCTTAAATTTTGCTGCTAAAATTTTTACCAATATCACTCAA
GATCATTTAGATTTTCACGGTACTTTTGAAAATTATAAAGAAGCAAAAGAACTTTTTTTTACCGATGAGAGTTTAAAATT
TATCAACAAAGATGCTTTGGCTATTAAATTTAATGTGCGTAATGCTTTTACTTATGGCATAGAAAATCCTGCTTTATATC
AAATCAAAGCTTATTCTTTAGAAGAAGGCATTAGTGCTATTGTAACAAATAAAAATCAAACTTTTCACATAGACTCGCCC
TTGCTAGGACTTTTTAATCTTTACAATCTTCTAGTTGCTAGTGCTTGTGTGAATGAACTCGTAAAGCCTGATTTAAAAGA
CTTGGAAAAAGCTATCAGTGGATTTGGCGGGGTTTGTGGCAGGGTAGAACAAGTAGCAAAAGGTGTGATTGTAGATTTTG
CTCATACGCCTGATGGCATTGAAAAAGTGCTTGACACTCTTAAAAATAAAAAACTCATCGTTGTTTTTGGAGCAGGTGGA
GATAGAGATAAAACCAAACGCCCTTTAATGGGAAAAATCGTCGAACATTTTGCAAAAATTGCTATCATTACAAGTGACAA
TCCACGCAGCGAAGAACCAAAAACCATCATGGAAGAAATTTTATCAGGCTTTACTAAAAAAGAAAAAGTTTTAATGATAG
AAGATAGAAAAGAAGCTATAAAAAAAGCTTTAGAACTAAAAGAAAATGACGATTTAGTCGTTATTTTAGGCAAAGGTGAT
GAAACTACTCAAGAAATTAAAGGGATAAAATATCCTTTTAGTGATAAAGTAGTTGTTAATGAAATTTTAAAAAATCAAGG
ATAA

Upstream 100 bases:

>100_bases
TATAAAGATATGACTTTAAATTATATAGAAAATGTTATGCCTCATTTTTGGGCAAATGATAGATTTATCAAACTCCAAGA
AAAACTCATGGGATTTAAAA

Downstream 100 bases:

>100_bases
TCAATGTTTGAAAATATGGATTTTTCTAAAATGGGTGAACTTTTAAATCAAGTTCAAGAAAAAGCAAAAAATATAGAACT
AGAACTTGCAAATCGTGAAT

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MKLKLENSFITDNTLECEKECFFLQTTQNAKFHAQALEKGAKIIDVNECKKLLKIDEKIQIIGITGTNGKTTTAAAIYSI
LLDLGYKCGLCGTRGAFINDEQIDEKSLTTSPILKTLEYLQIATQKKCDFFIMEVSSHALIQNRIEGLNFAAKIFTNITQ
DHLDFHGTFENYKEAKELFFTDESLKFINKDALAIKFNVRNAFTYGIENPALYQIKAYSLEEGISAIVTNKNQTFHIDSP
LLGLFNLYNLLVASACVNELVKPDLKDLEKAISGFGGVCGRVEQVAKGVIVDFAHTPDGIEKVLDTLKNKKLIVVFGAGG
DRDKTKRPLMGKIVEHFAKIAIITSDNPRSEEPKTIMEEILSGFTKKEKVLMIEDRKEAIKKALELKENDDLVVILGKGD
ETTQEIKGIKYPFSDKVVVNEILKNQG

Sequences:

>Translated_427_residues
MKLKLENSFITDNTLECEKECFFLQTTQNAKFHAQALEKGAKIIDVNECKKLLKIDEKIQIIGITGTNGKTTTAAAIYSI
LLDLGYKCGLCGTRGAFINDEQIDEKSLTTSPILKTLEYLQIATQKKCDFFIMEVSSHALIQNRIEGLNFAAKIFTNITQ
DHLDFHGTFENYKEAKELFFTDESLKFINKDALAIKFNVRNAFTYGIENPALYQIKAYSLEEGISAIVTNKNQTFHIDSP
LLGLFNLYNLLVASACVNELVKPDLKDLEKAISGFGGVCGRVEQVAKGVIVDFAHTPDGIEKVLDTLKNKKLIVVFGAGG
DRDKTKRPLMGKIVEHFAKIAIITSDNPRSEEPKTIMEEILSGFTKKEKVLMIEDRKEAIKKALELKENDDLVVILGKGD
ETTQEIKGIKYPFSDKVVVNEILKNQG
>Mature_427_residues
MKLKLENSFITDNTLECEKECFFLQTTQNAKFHAQALEKGAKIIDVNECKKLLKIDEKIQIIGITGTNGKTTTAAAIYSI
LLDLGYKCGLCGTRGAFINDEQIDEKSLTTSPILKTLEYLQIATQKKCDFFIMEVSSHALIQNRIEGLNFAAKIFTNITQ
DHLDFHGTFENYKEAKELFFTDESLKFINKDALAIKFNVRNAFTYGIENPALYQIKAYSLEEGISAIVTNKNQTFHIDSP
LLGLFNLYNLLVASACVNELVKPDLKDLEKAISGFGGVCGRVEQVAKGVIVDFAHTPDGIEKVLDTLKNKKLIVVFGAGG
DRDKTKRPLMGKIVEHFAKIAIITSDNPRSEEPKTIMEEILSGFTKKEKVLMIEDRKEAIKKALELKENDDLVVILGKGD
ETTQEIKGIKYPFSDKVVVNEILKNQG

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=360, Percent_Identity=35.5555555555556, Blast_Score=201, Evalue=6e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 47706; Mature: 47706

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKLENSFITDNTLECEKECFFLQTTQNAKFHAQALEKGAKIIDVNECKKLLKIDEKIQ
CEEEECCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCEEEEHHHHHHHHCCCCEEE
IIGITGTNGKTTTAAAIYSILLDLGYKCGLCGTRGAFINDEQIDEKSLTTSPILKTLEYL
EEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHH
QIATQKKCDFFIMEVSSHALIQNRIEGLNFAAKIFTNITQDHLDFHGTFENYKEAKELFF
HHHHCCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
TDESLKFINKDALAIKFNVRNAFTYGIENPALYQIKAYSLEEGISAIVTNKNQTFHIDSP
CCHHHHHHCCCEEEEEEEECCEEEECCCCCEEEEEEEECHHCCCCEEEECCCCEEEECCH
LLGLFNLYNLLVASACVNELVKPDLKDLEKAISGFGGVCGRVEQVAKGVIVDFAHTPDGI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHCCEEEEECCCCHHH
EKVLDTLKNKKLIVVFGAGGDRDKTKRPLMGKIVEHFAKIAIITSDNPRSEEPKTIMEEI
HHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHH
LSGFTKKEKVLMIEDRKEAIKKALELKENDDLVVILGKGDETTQEIKGIKYPFSDKVVVN
HHCCCCCCEEEEEECHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHCCCCCCCCCHHHHH
EILKNQG
HHHHCCC
>Mature Secondary Structure
MKLKLENSFITDNTLECEKECFFLQTTQNAKFHAQALEKGAKIIDVNECKKLLKIDEKIQ
CEEEECCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCEEEEHHHHHHHHCCCCEEE
IIGITGTNGKTTTAAAIYSILLDLGYKCGLCGTRGAFINDEQIDEKSLTTSPILKTLEYL
EEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHHHHH
QIATQKKCDFFIMEVSSHALIQNRIEGLNFAAKIFTNITQDHLDFHGTFENYKEAKELFF
HHHHCCCCCEEEEECCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHC
TDESLKFINKDALAIKFNVRNAFTYGIENPALYQIKAYSLEEGISAIVTNKNQTFHIDSP
CCHHHHHHCCCEEEEEEEECCEEEECCCCCEEEEEEEECHHCCCCEEEECCCCEEEECCH
LLGLFNLYNLLVASACVNELVKPDLKDLEKAISGFGGVCGRVEQVAKGVIVDFAHTPDGI
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHHHHHCCEEEEECCCCHHH
EKVLDTLKNKKLIVVFGAGGDRDKTKRPLMGKIVEHFAKIAIITSDNPRSEEPKTIMEEI
HHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHH
LSGFTKKEKVLMIEDRKEAIKKALELKENDDLVVILGKGDETTQEIKGIKYPFSDKVVVN
HHCCCCCCEEEEEECHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHCCCCCCCCCHHHHH
EILKNQG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10688204 [H]