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The map label for this gene is kgtP [H]

Identifier: 57238631

GI number: 57238631

Start: 1687671

End: 1688930

Strand: Direct

Name: kgtP [H]

Synonym: CJE1791

Alternate gene names: 57238631

Gene position: 1687671-1688930 (Clockwise)

Preceding gene: 57238630

Following gene: 57238633

Centisome position: 94.93

GC content: 33.17

Gene sequence:

>1260_bases
ATGCAAGAGCTTACTCGCACGAAAAAAATTCGCTCTATTATTGCTGCAAGTAGCGGAAATTTAGTAGAGTGGTTTGATTT
TTATATCTATGCTTTTACAGCAACTTATTTTGCTCATACTTTTTCAACTTCAGATAATCCTGTTATCCAGCAAATCAATG
CCTTTGGGGTTTTTGCAGCAGGGTTTTTTATGCGTCCTATAGGCTCTTGGCTTTTTGGCTCTTTAGCTGATAAAGTAGGT
CGTAAAAAATCTATGGTTATATCAGTGGTTTTAATGGCTTTGGGCTCTTTTATGATAGCAGCCTTACCAAGCAAAGATAC
AGTGGGCGATCTTGCTATTATCTTACTTTTAATAGCAAGACTCATTCAAGGTCTTAGTGTAGGCGGGGAGTATGGCATAG
CAGCTACTTATCTTTCAGAACTTGCTACAGAAGGAAAACGCGGTTTTTACTCATCTTTTCAGTATGTAACACTAATAGGT
GGTCAACTTTTGGCCGTTGCAAGCATTAGTATAATGTTTTTATTTTTTAGCATTGATGATATGAAAGATTATGCTTGGAG
AATACTTTTTGTAATAGGCGGAATTTTAGCACTAGGCTCGCTTTTTGTAAGAAAAATGATGAATGAAAGTGCTACAAAAC
TTCACCAGCATGAAGATCGTGGTACTTTAAAAGCATTATGCAAATCTTGGAAACCTTTTTTAATGGTGCTTGGGGTTACT
TCTGGAGGTTCTTTAGCTTTTTATACTATTACAACTTATACTAAAACTTTTATGGAAAATACGGGTATGGATAAAATGCT
TGTTAATAATCTTTTCTTAGGTGCTTTATTTATACTAATGATCATTCAACCTCTTTTTGGATATATAGGAGATAAAATAG
GACACAAACGATCTTTAATTGTATTTTGTATTTTGGCATTGGTAGGAATTTATCCTATTTTTTCACTTATAGCCAATAAC
GCACAAAATAATCCTTCTTTGACATTTATGTTAGTAGTATTATTGTTTGTTATTTTAAGTTTTTATACTTCAGTTGCTGG
GATTTTTAAAGCAAAACTTTTTCCTGAACATGTAAGAGCTTTAGGGACAGGTTTGGGTTATGCTATTTCAAATGCTGTTT
TTGGAGGATCTGCTCCTTGGGTTGCTTTACAATTTAAAAATGCAGGTATAGAAAATGGCTTTTTTATATATATAGCTATT
TTTACAATCATCATGCTTATAGCTACTTTATGTTTGCCTAAAAAATCAGAGCTTAATTAA

Upstream 100 bases:

>100_bases
TCGATATTTTTATCTTTTTTATTTCTCTTCTTTATTTTTAAATATATTAAACAAAAATCACACTATACTTAAATAAAAAT
CTAAAATTAAGGAAGGGTTT

Downstream 100 bases:

>100_bases
TTTTAACGCTTTTAAAGAAAGTGCCGAAAGTGCGGTACTTTCTAACTCTTTTAAGGTTTTAAATTCATAATTTTTATTTT
CATTGTTTAAAATTTGATGA

Product: alpha-ketoglutarate permease

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MQELTRTKKIRSIIAASSGNLVEWFDFYIYAFTATYFAHTFSTSDNPVIQQINAFGVFAAGFFMRPIGSWLFGSLADKVG
RKKSMVISVVLMALGSFMIAALPSKDTVGDLAIILLLIARLIQGLSVGGEYGIAATYLSELATEGKRGFYSSFQYVTLIG
GQLLAVASISIMFLFFSIDDMKDYAWRILFVIGGILALGSLFVRKMMNESATKLHQHEDRGTLKALCKSWKPFLMVLGVT
SGGSLAFYTITTYTKTFMENTGMDKMLVNNLFLGALFILMIIQPLFGYIGDKIGHKRSLIVFCILALVGIYPIFSLIANN
AQNNPSLTFMLVVLLFVILSFYTSVAGIFKAKLFPEHVRALGTGLGYAISNAVFGGSAPWVALQFKNAGIENGFFIYIAI
FTIIMLIATLCLPKKSELN

Sequences:

>Translated_419_residues
MQELTRTKKIRSIIAASSGNLVEWFDFYIYAFTATYFAHTFSTSDNPVIQQINAFGVFAAGFFMRPIGSWLFGSLADKVG
RKKSMVISVVLMALGSFMIAALPSKDTVGDLAIILLLIARLIQGLSVGGEYGIAATYLSELATEGKRGFYSSFQYVTLIG
GQLLAVASISIMFLFFSIDDMKDYAWRILFVIGGILALGSLFVRKMMNESATKLHQHEDRGTLKALCKSWKPFLMVLGVT
SGGSLAFYTITTYTKTFMENTGMDKMLVNNLFLGALFILMIIQPLFGYIGDKIGHKRSLIVFCILALVGIYPIFSLIANN
AQNNPSLTFMLVVLLFVILSFYTSVAGIFKAKLFPEHVRALGTGLGYAISNAVFGGSAPWVALQFKNAGIENGFFIYIAI
FTIIMLIATLCLPKKSELN
>Mature_419_residues
MQELTRTKKIRSIIAASSGNLVEWFDFYIYAFTATYFAHTFSTSDNPVIQQINAFGVFAAGFFMRPIGSWLFGSLADKVG
RKKSMVISVVLMALGSFMIAALPSKDTVGDLAIILLLIARLIQGLSVGGEYGIAATYLSELATEGKRGFYSSFQYVTLIG
GQLLAVASISIMFLFFSIDDMKDYAWRILFVIGGILALGSLFVRKMMNESATKLHQHEDRGTLKALCKSWKPFLMVLGVT
SGGSLAFYTITTYTKTFMENTGMDKMLVNNLFLGALFILMIIQPLFGYIGDKIGHKRSLIVFCILALVGIYPIFSLIANN
AQNNPSLTFMLVVLLFVILSFYTSVAGIFKAKLFPEHVRALGTGLGYAISNAVFGGSAPWVALQFKNAGIENGFFIYIAI
FTIIMLIATLCLPKKSELN

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=416, Percent_Identity=49.0384615384615, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI1790550, Length=419, Percent_Identity=29.5942720763723, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1788292, Length=390, Percent_Identity=30.5128205128205, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1789941, Length=400, Percent_Identity=29.5, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI87082404, Length=342, Percent_Identity=27.1929824561404, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082231, Length=226, Percent_Identity=27.8761061946903, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46031; Mature: 46031

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQELTRTKKIRSIIAASSGNLVEWFDFYIYAFTATYFAHTFSTSDNPVIQQINAFGVFAA
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GFFMRPIGSWLFGSLADKVGRKKSMVISVVLMALGSFMIAALPSKDTVGDLAIILLLIAR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LIQGLSVGGEYGIAATYLSELATEGKRGFYSSFQYVTLIGGQLLAVASISIMFLFFSIDD
HHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKDYAWRILFVIGGILALGSLFVRKMMNESATKLHQHEDRGTLKALCKSWKPFLMVLGVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEEC
SGGSLAFYTITTYTKTFMENTGMDKMLVNNLFLGALFILMIIQPLFGYIGDKIGHKRSLI
CCCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VFCILALVGIYPIFSLIANNAQNNPSLTFMLVVLLFVILSFYTSVAGIFKAKLFPEHVRA
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
LGTGLGYAISNAVFGGSAPWVALQFKNAGIENGFFIYIAIFTIIMLIATLCLPKKSELN
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQELTRTKKIRSIIAASSGNLVEWFDFYIYAFTATYFAHTFSTSDNPVIQQINAFGVFAA
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
GFFMRPIGSWLFGSLADKVGRKKSMVISVVLMALGSFMIAALPSKDTVGDLAIILLLIAR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
LIQGLSVGGEYGIAATYLSELATEGKRGFYSSFQYVTLIGGQLLAVASISIMFLFFSIDD
HHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MKDYAWRILFVIGGILALGSLFVRKMMNESATKLHQHEDRGTLKALCKSWKPFLMVLGVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEEEC
SGGSLAFYTITTYTKTFMENTGMDKMLVNNLFLGALFILMIIQPLFGYIGDKIGHKRSLI
CCCCEEEEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
VFCILALVGIYPIFSLIANNAQNNPSLTFMLVVLLFVILSFYTSVAGIFKAKLFPEHVRA
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
LGTGLGYAISNAVFGGSAPWVALQFKNAGIENGFFIYIAIFTIIMLIATLCLPKKSELN
HHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]