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The map label for this gene is hsdM [H]

Identifier: 57238568

GI number: 57238568

Start: 1625194

End: 1626678

Strand: Direct

Name: hsdM [H]

Synonym: CJE1724

Alternate gene names: 57238568

Gene position: 1625194-1626678 (Clockwise)

Preceding gene: 57238567

Following gene: 57238570

Centisome position: 91.41

GC content: 30.03

Gene sequence:

>1485_bases
ATGCAAAATAAAGTCGATAAAATCACAGATATTCTAAGAAGAGATGATGGCATAAGTGGTGCTATGCACTATACAGAGCA
GATTGGCTGGATACTTTTTTTAAAATTTTTAGATGATTATGAGACAAATTTAAAAGATTTAGCGTTTTTAGATGGCAAAG
ATTATAAAAGCATTTTGCAAGAAAAATTTAGTTGGAGTGTTTGGGCAGCGCCTAAAAAAGATGGCAAACTTGATGTAAAA
AATGCTTTAAGTGGATCGGATTTGCTTGAATTTGTAAATAAAGAGCTTTTTCCTTATCTTAAAAATTTTAAAAATAATGA
TGATTTTAAAAGTATAGAGTATAAAATCGGTGGAATTTTTGAATTTATAGACAATCGCATAGCAAATGGACATACTTTAA
GAGAAGTGATAAATATCATTGATGAGATTAGTTTTAATAAAGAAGATGAAGTATTTGCCTTAGGTGAAGTTTATGAAAAA
CTTCTAAAAGATATGGGTAGCGATGGTGGAAATAGTGGAGAATTTTACACCCCTCGTCCTTTGATAAAGGCTATGGTGGA
AGTAATAGATCCTAAACCAAAAGAAAGAATTTATGATCCTTCTTGTGGTAGTTGTGGATTTTTAGTTGAGAGTTTTTTAC
ATATTTTATATAAAGATAGAACCAAAGGCAAAAAAGCAAATTTAAGCGTAGAAGAGCTTGAATTTTTAAAAAATGATGCT
CTTTTTGGTAAAGAAAAAACTCCGCTTAGCTACGCAATGGGCGTGATGAATATGATCTTGCACGAGGTAAAATCTCCAAA
TATCATCAAAACAAACACTCTAAATAAAAAAATCACAGACATAACCCAAAGCGAAAAATACGAAGTGATTTTGGCAAATC
CACCCTTTGGAGGTAAAGAAAAAGAACAAATTCAAGAAAATTTTCCTATAAAATCAAACGCTACTGAACTTTTGTTTTTA
CAACACATTTTAAAATCTTTAAAAAACAACGGAAGATGTGCGATCATCGTGCCTGAAGGTGTGCTTTTTCAAAATTCAAA
TGCCTTTGTGAGTGTAAAAAAAGATTTACTAGATGATTTTAATTTAGAATGCGTTTTGAGTTTGCCAAGTGGGGTATTTT
TGCCTTATAGTGCTGTTAAGACTAATGTGCTTTTTTTCTCTAAAGGTAAAAAATGTATTTGTGAAGGCGATGGAGTTTAT
TACTACGAACTTATACCGCCTTATAAACTTACTAAAAACAAGCCTTTAGAATACACACATTTTAAAGAATTTCTAAAGTG
CTACAAAGAAAGAAAAATCACTGCTAATTCTTGGCTTGTTAGCAAAAAAGAGCTTGAAGAAAGAAATTATGATTTAAGTG
CTAAAAATCCAAATGTAAAAGAAGAAAAAATCCTAAAAACTAGTGAAGAGATTTTAAATTCTTTAGAAGAAAATCTAAAA
ATACAACAAGAATATCTAAATGAGCTTAAAAGCATACTAAAATGA

Upstream 100 bases:

>100_bases
TTGTGAAAATTTAGGAATATTAGAGTGTATTAAAATGGGTAGAAGTAATTATTATATAAATATAGAACTTTTAAGACTTT
TTAGAAAAGGAATTTTTTAA

Downstream 100 bases:

>100_bases
TTGCAAGTTTTTTGATAAAAACTAATTTACAAATAACTTCTAAGCTCAATTTACAATAAATATTTATTGCTTGCATTCTA
CTTTTTAATATCATCAAGCA

Product: type I restriction-modification system subunit M

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MQNKVDKITDILRRDDGISGAMHYTEQIGWILFLKFLDDYETNLKDLAFLDGKDYKSILQEKFSWSVWAAPKKDGKLDVK
NALSGSDLLEFVNKELFPYLKNFKNNDDFKSIEYKIGGIFEFIDNRIANGHTLREVINIIDEISFNKEDEVFALGEVYEK
LLKDMGSDGGNSGEFYTPRPLIKAMVEVIDPKPKERIYDPSCGSCGFLVESFLHILYKDRTKGKKANLSVEELEFLKNDA
LFGKEKTPLSYAMGVMNMILHEVKSPNIIKTNTLNKKITDITQSEKYEVILANPPFGGKEKEQIQENFPIKSNATELLFL
QHILKSLKNNGRCAIIVPEGVLFQNSNAFVSVKKDLLDDFNLECVLSLPSGVFLPYSAVKTNVLFFSKGKKCICEGDGVY
YYELIPPYKLTKNKPLEYTHFKEFLKCYKERKITANSWLVSKKELEERNYDLSAKNPNVKEEKILKTSEEILNSLEENLK
IQQEYLNELKSILK

Sequences:

>Translated_494_residues
MQNKVDKITDILRRDDGISGAMHYTEQIGWILFLKFLDDYETNLKDLAFLDGKDYKSILQEKFSWSVWAAPKKDGKLDVK
NALSGSDLLEFVNKELFPYLKNFKNNDDFKSIEYKIGGIFEFIDNRIANGHTLREVINIIDEISFNKEDEVFALGEVYEK
LLKDMGSDGGNSGEFYTPRPLIKAMVEVIDPKPKERIYDPSCGSCGFLVESFLHILYKDRTKGKKANLSVEELEFLKNDA
LFGKEKTPLSYAMGVMNMILHEVKSPNIIKTNTLNKKITDITQSEKYEVILANPPFGGKEKEQIQENFPIKSNATELLFL
QHILKSLKNNGRCAIIVPEGVLFQNSNAFVSVKKDLLDDFNLECVLSLPSGVFLPYSAVKTNVLFFSKGKKCICEGDGVY
YYELIPPYKLTKNKPLEYTHFKEFLKCYKERKITANSWLVSKKELEERNYDLSAKNPNVKEEKILKTSEEILNSLEENLK
IQQEYLNELKSILK
>Mature_494_residues
MQNKVDKITDILRRDDGISGAMHYTEQIGWILFLKFLDDYETNLKDLAFLDGKDYKSILQEKFSWSVWAAPKKDGKLDVK
NALSGSDLLEFVNKELFPYLKNFKNNDDFKSIEYKIGGIFEFIDNRIANGHTLREVINIIDEISFNKEDEVFALGEVYEK
LLKDMGSDGGNSGEFYTPRPLIKAMVEVIDPKPKERIYDPSCGSCGFLVESFLHILYKDRTKGKKANLSVEELEFLKNDA
LFGKEKTPLSYAMGVMNMILHEVKSPNIIKTNTLNKKITDITQSEKYEVILANPPFGGKEKEQIQENFPIKSNATELLFL
QHILKSLKNNGRCAIIVPEGVLFQNSNAFVSVKKDLLDDFNLECVLSLPSGVFLPYSAVKTNVLFFSKGKKCICEGDGVY
YYELIPPYKLTKNKPLEYTHFKEFLKCYKERKITANSWLVSKKELEERNYDLSAKNPNVKEEKILKTSEEILNSLEENLK
IQQEYLNELKSILK

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=449, Percent_Identity=28.2850779510022, Blast_Score=158, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 56742; Mature: 56742

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNKVDKITDILRRDDGISGAMHYTEQIGWILFLKFLDDYETNLKDLAFLDGKDYKSILQ
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCHHHHHHHH
EKFSWSVWAAPKKDGKLDVKNALSGSDLLEFVNKELFPYLKNFKNNDDFKSIEYKIGGIF
HHHCCEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
EFIDNRIANGHTLREVINIIDEISFNKEDEVFALGEVYEKLLKDMGSDGGNSGEFYTPRP
HHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCHH
LIKAMVEVIDPKPKERIYDPSCGSCGFLVESFLHILYKDRTKGKKANLSVEELEFLKNDA
HHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
LFGKEKTPLSYAMGVMNMILHEVKSPNIIKTNTLNKKITDITQSEKYEVILANPPFGGKE
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCEEEEEECCCCCCCH
KEQIQENFPIKSNATELLFLQHILKSLKNNGRCAIIVPEGVLFQNSNAFVSVKKDLLDDF
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCEEEEHHHHHCCC
NLECVLSLPSGVFLPYSAVKTNVLFFSKGKKCICEGDGVYYYELIPPYKLTKNKPLEYTH
CCEEEEECCCCCCCCHHHHHHEEEEEECCCEEEECCCCEEEEEECCCEECCCCCCCCHHH
FKEFLKCYKERKITANSWLVSKKELEERNYDLSAKNPNVKEEKILKTSEEILNSLEENLK
HHHHHHHHHHCCCCHHHHEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IQQEYLNELKSILK
HHHHHHHHHHHHHC
>Mature Secondary Structure
MQNKVDKITDILRRDDGISGAMHYTEQIGWILFLKFLDDYETNLKDLAFLDGKDYKSILQ
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECCHHHHHHHH
EKFSWSVWAAPKKDGKLDVKNALSGSDLLEFVNKELFPYLKNFKNNDDFKSIEYKIGGIF
HHHCCEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
EFIDNRIANGHTLREVINIIDEISFNKEDEVFALGEVYEKLLKDMGSDGGNSGEFYTPRP
HHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHCCCCCCCCCCCCCCHH
LIKAMVEVIDPKPKERIYDPSCGSCGFLVESFLHILYKDRTKGKKANLSVEELEFLKNDA
HHHHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCC
LFGKEKTPLSYAMGVMNMILHEVKSPNIIKTNTLNKKITDITQSEKYEVILANPPFGGKE
CCCCCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCEEEEEECCCCCCCH
KEQIQENFPIKSNATELLFLQHILKSLKNNGRCAIIVPEGVLFQNSNAFVSVKKDLLDDF
HHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCCCEEEEHHHHHCCC
NLECVLSLPSGVFLPYSAVKTNVLFFSKGKKCICEGDGVYYYELIPPYKLTKNKPLEYTH
CCEEEEECCCCCCCCHHHHHHEEEEEECCCEEEECCCCEEEEEECCCEECCCCCCCCHHH
FKEFLKCYKERKITANSWLVSKKELEERNYDLSAKNPNVKEEKILKTSEEILNSLEENLK
HHHHHHHHHHCCCCHHHHEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
IQQEYLNELKSILK
HHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]