Click here to switch to the map view.

The map label for this gene is dapF [H]

Identifier: 57238549

GI number: 57238549

Start: 1602025

End: 1602774

Strand: Direct

Name: dapF [H]

Synonym: CJE1702

Alternate gene names: 57238549

Gene position: 1602025-1602774 (Clockwise)

Preceding gene: 57238548

Following gene: 57238550

Centisome position: 90.11

GC content: 32.27

Gene sequence:

>750_bases
GTGAAATTTTATAAATATTGTGCAAGTGGGAATGATTTTGTCATAACAAATGCTGATAGAAAAGAAGATAGAAGTGCTTT
AGCTAAAGAACTTTGCAATCGTTATGAGGGCATAGGTGGGGATGGCTTTATCGTGATATTGCCTCATGAAAAATACGACT
TTGAATGGGAATTTTATAATAACGATGGCTCAAGGGCTGCTATGTGTGGCAATGGCTCAAGGGCTGCGGCACATTTTGCA
CATCATATTAACAAAATAAATCCTAACATGAGTTTTTTAACCGGTGCAGGAATAATAAAAGCAAAGGTAAATCAAGATAA
AGTTGAAGTATCTTTAGGCAAAATAAAAAGCGTGCAAAATACTTTTGAAGAATTAGGAAAAACTTGGCAACTTTGCGACA
CTGGAGTTCCACATCTAGTGCATTTTTGTCAAAATTTAGATGAATTTGATACAATACTTTGTCAAAAAATGAGACAAAAA
TATAATGCCAATGTTAATTTTGTAAAAATTTTAGATGAAAATCATTTAAAAGTTCGTACCTATGAACGCGGAGTTGAAGA
TGAAACCTTAGCTTGTGGCACAGGAATGGGGGCTTGTTTTTATCTGGCATTTTTAAATAAAAAAGTACAAAATAAAGTTA
AAATAACTCCTAAAAGTGGTGAAGAGGTTGGGTTTACATATAAAAATGAAGAATTGTTTTTTGAAGGAAAGGTAAAGTAT
TGTTTTGAAGCCAATTATAATTTTTCTTAG

Upstream 100 bases:

>100_bases
AACTTAAAAAAGCAGATTTTATCATTAAAAATACTAATTCTTATGTCGATTTTAGGCAAGAATGTGTTAAAGTAATACAA
GAAATTTCTAAAGGAAAAAT

Downstream 100 bases:

>100_bases
TCTTTTTCTAATCCCACTTTTTGCTGATGATCTAAAAAATGGCTTTGGAGAGGAATATTACAAACTTGATATAGATCAAA
AAAGACAAATTTTTTTTATA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MKFYKYCASGNDFVITNADRKEDRSALAKELCNRYEGIGGDGFIVILPHEKYDFEWEFYNNDGSRAAMCGNGSRAAAHFA
HHINKINPNMSFLTGAGIIKAKVNQDKVEVSLGKIKSVQNTFEELGKTWQLCDTGVPHLVHFCQNLDEFDTILCQKMRQK
YNANVNFVKILDENHLKVRTYERGVEDETLACGTGMGACFYLAFLNKKVQNKVKITPKSGEEVGFTYKNEELFFEGKVKY
CFEANYNFS

Sequences:

>Translated_249_residues
MKFYKYCASGNDFVITNADRKEDRSALAKELCNRYEGIGGDGFIVILPHEKYDFEWEFYNNDGSRAAMCGNGSRAAAHFA
HHINKINPNMSFLTGAGIIKAKVNQDKVEVSLGKIKSVQNTFEELGKTWQLCDTGVPHLVHFCQNLDEFDTILCQKMRQK
YNANVNFVKILDENHLKVRTYERGVEDETLACGTGMGACFYLAFLNKKVQNKVKITPKSGEEVGFTYKNEELFFEGKVKY
CFEANYNFS
>Mature_249_residues
MKFYKYCASGNDFVITNADRKEDRSALAKELCNRYEGIGGDGFIVILPHEKYDFEWEFYNNDGSRAAMCGNGSRAAAHFA
HHINKINPNMSFLTGAGIIKAKVNQDKVEVSLGKIKSVQNTFEELGKTWQLCDTGVPHLVHFCQNLDEFDTILCQKMRQK
YNANVNFVKILDENHLKVRTYERGVEDETLACGTGMGACFYLAFLNKKVQNKVKITPKSGEEVGFTYKNEELFFEGKVKY
CFEANYNFS

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=271, Percent_Identity=25.0922509225092, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 28349; Mature: 28349

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFYKYCASGNDFVITNADRKEDRSALAKELCNRYEGIGGDGFIVILPHEKYDFEWEFYN
CCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEC
NDGSRAAMCGNGSRAAAHFAHHINKINPNMSFLTGAGIIKAKVNQDKVEVSLGKIKSVQN
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCCEEEHHHHHHHHHH
TFEELGKTWQLCDTGVPHLVHFCQNLDEFDTILCQKMRQKYNANVNFVKILDENHLKVRT
HHHHHCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEE
YERGVEDETLACGTGMGACFYLAFLNKKVQNKVKITPKSGEEVGFTYKNEELFFEGKVKY
ECCCCCCCEEECCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEECCEEEEECCEEE
CFEANYNFS
EEECCCCCC
>Mature Secondary Structure
MKFYKYCASGNDFVITNADRKEDRSALAKELCNRYEGIGGDGFIVILPHEKYDFEWEFYN
CCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEC
NDGSRAAMCGNGSRAAAHFAHHINKINPNMSFLTGAGIIKAKVNQDKVEVSLGKIKSVQN
CCCCEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCCEEEHHHHHHHHHH
TFEELGKTWQLCDTGVPHLVHFCQNLDEFDTILCQKMRQKYNANVNFVKILDENHLKVRT
HHHHHCCCHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEE
YERGVEDETLACGTGMGACFYLAFLNKKVQNKVKITPKSGEEVGFTYKNEELFFEGKVKY
ECCCCCCCEEECCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEECCEEEEECCEEE
CFEANYNFS
EEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA