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The map label for this gene is prfB [H]

Identifier: 57238478

GI number: 57238478

Start: 1531165

End: 1532262

Strand: Direct

Name: prfB [H]

Synonym: CJE1628

Alternate gene names: 57238478

Gene position: 1531165-1532262 (Clockwise)

Preceding gene: 57238475

Following gene: 57238480

Centisome position: 86.13

GC content: 36.89

Gene sequence:

>1098_bases
ATGGATAATTACGAATTTAGCGAACTTTTAAAAACTTTAAAAAACAAAGTCGGCAACATAGCCTCCATCATCAAGCCTGA
AAATATCCAAACAAGGCTTAAAGAAATCGAAGAGCTTGAAAATTCTCCTTCTTTTTGGAGTGATGTTAAACAAGCAGGAG
TCATAGGTAAAGAAAAAACTAAAATCACCAATCTACTTAAAAACTATGAAAATGCTTTTAATGCTCTAAATGACGCAAGC
GAACTTTTCGATCTGGCTAATAGCGAAAACGATACAGAAACACTTGAAGCTTTATTTAATGACGCACCAAAACTTGAAGA
TACCATCACAAGTCTTGAAATTTCCATGCTTTTAAGCGGGGAAAATGATGGTAAAAATGCCATTGTTTCCATTCACCCAG
GTGCAGGTGGAACGGAAAGTAATGACTGGGCAAGCATACTTTATAGAATGTATTTAAGATTTTGTGAAAGAGAAGGTTTT
AAAGTCGAAACTTTAGACTTTCAAGAAGGCGAAGAAGCAGGACTTAAAGATGTGAGCTTTTTAGTTAAAGGAGAAAATGC
TTATGGTTATTTGAAGGCTGAAAATGGTATCCATCGTTTGGTAAGAACTTCTCCTTTTGATAGTGCAGGACGCCGTCATA
CAAGCTTTTCAAGTGTTATGGTAAGCCCTGAACTTGATGATGATATAGAAATTGAAATCGAAGAAAAAGACATAAGAATA
GATTATTACAGAGCAAGCGGTGCAGGCGGACAACATGTCAATAAAACAGAATCGGCTGTGAGAATCACGCATTTTCCAAC
CGGCATAGTCGTACAATGCCAAAATGACAGAAGTCAGCACAAAAACAAAGCCACCGCTTTTAAAATGCTAAAATCTCGCC
TTTATGAACTTGAACTCATGAAACAACAAGATAGTGCTAATACTGGAGAAAAAAGCGAGATAGGTTGGGGGCATCAAATC
CGTTCTTATGTGCTTTTCCCTTATCAGCAAGTCAAAGACAATCGCAGCGGAGAAGCTTTTTCTCAAGTGGATAATATCCT
TGATGGGGATATCAAAAAAATGATAGAAGGCGTTTTAATTTCCTTAAAAGCGGAGTAA

Upstream 100 bases:

>100_bases
TAAAGACATAAGATAAAGTTTTGACATTTTTTTCCTATATTAAAGTTTTCTTTTGATATAATTTTTGACTTTAAAAATAT
AATTATAGCAAAGAAATTTT

Downstream 100 bases:

>100_bases
CAGGCTTAAAAATAAGTAATTTCTGCAAAATGTCGGTATAAAAAAATTAGAAAGATAGCGAGATTTTCCCGCTTGTTTTA
TTATCTTGCATAAAACCTAA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MDNYEFSELLKTLKNKVGNIASIIKPENIQTRLKEIEELENSPSFWSDVKQAGVIGKEKTKITNLLKNYENAFNALNDAS
ELFDLANSENDTETLEALFNDAPKLEDTITSLEISMLLSGENDGKNAIVSIHPGAGGTESNDWASILYRMYLRFCEREGF
KVETLDFQEGEEAGLKDVSFLVKGENAYGYLKAENGIHRLVRTSPFDSAGRRHTSFSSVMVSPELDDDIEIEIEEKDIRI
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKATAFKMLKSRLYELELMKQQDSANTGEKSEIGWGHQI
RSYVLFPYQQVKDNRSGEAFSQVDNILDGDIKKMIEGVLISLKAE

Sequences:

>Translated_365_residues
MDNYEFSELLKTLKNKVGNIASIIKPENIQTRLKEIEELENSPSFWSDVKQAGVIGKEKTKITNLLKNYENAFNALNDAS
ELFDLANSENDTETLEALFNDAPKLEDTITSLEISMLLSGENDGKNAIVSIHPGAGGTESNDWASILYRMYLRFCEREGF
KVETLDFQEGEEAGLKDVSFLVKGENAYGYLKAENGIHRLVRTSPFDSAGRRHTSFSSVMVSPELDDDIEIEIEEKDIRI
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKATAFKMLKSRLYELELMKQQDSANTGEKSEIGWGHQI
RSYVLFPYQQVKDNRSGEAFSQVDNILDGDIKKMIEGVLISLKAE
>Mature_365_residues
MDNYEFSELLKTLKNKVGNIASIIKPENIQTRLKEIEELENSPSFWSDVKQAGVIGKEKTKITNLLKNYENAFNALNDAS
ELFDLANSENDTETLEALFNDAPKLEDTITSLEISMLLSGENDGKNAIVSIHPGAGGTESNDWASILYRMYLRFCEREGF
KVETLDFQEGEEAGLKDVSFLVKGENAYGYLKAENGIHRLVRTSPFDSAGRRHTSFSSVMVSPELDDDIEIEIEEKDIRI
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKATAFKMLKSRLYELELMKQQDSANTGEKSEIGWGHQI
RSYVLFPYQQVKDNRSGEAFSQVDNILDGDIKKMIEGVLISLKAE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=271, Percent_Identity=35.7933579335793, Blast_Score=165, Evalue=6e-41,
Organism=Homo sapiens, GI34577120, Length=323, Percent_Identity=31.2693498452012, Blast_Score=148, Evalue=7e-36,
Organism=Homo sapiens, GI166795305, Length=172, Percent_Identity=38.3720930232558, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI2367172, Length=335, Percent_Identity=49.8507462686567, Blast_Score=330, Evalue=6e-92,
Organism=Escherichia coli, GI1787462, Length=247, Percent_Identity=40.8906882591093, Blast_Score=198, Evalue=5e-52,
Organism=Caenorhabditis elegans, GI17542784, Length=320, Percent_Identity=28.125, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=311, Percent_Identity=31.1897106109325, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI19921226, Length=238, Percent_Identity=38.2352941176471, Blast_Score=157, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 41070; Mature: 41070

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNYEFSELLKTLKNKVGNIASIIKPENIQTRLKEIEELENSPSFWSDVKQAGVIGKEKT
CCCCCHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHH
KITNLLKNYENAFNALNDASELFDLANSENDTETLEALFNDAPKLEDTITSLEISMLLSG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHEEEEEEEEC
ENDGKNAIVSIHPGAGGTESNDWASILYRMYLRFCEREGFKVETLDFQEGEEAGLKDVSF
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEE
LVKGENAYGYLKAENGIHRLVRTSPFDSAGRRHTSFSSVMVSPELDDDIEIEIEEKDIRI
EEECCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHEEECCCCCCCEEEEEECCCEEE
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKATAFKMLKSRLYELELM
EEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
KQQDSANTGEKSEIGWGHQIRSYVLFPYQQVKDNRSGEAFSQVDNILDGDIKKMIEGVLI
HHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHE
SLKAE
EEECC
>Mature Secondary Structure
MDNYEFSELLKTLKNKVGNIASIIKPENIQTRLKEIEELENSPSFWSDVKQAGVIGKEKT
CCCCCHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHH
KITNLLKNYENAFNALNDASELFDLANSENDTETLEALFNDAPKLEDTITSLEISMLLSG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHEEEEEEEEC
ENDGKNAIVSIHPGAGGTESNDWASILYRMYLRFCEREGFKVETLDFQEGEEAGLKDVSF
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEE
LVKGENAYGYLKAENGIHRLVRTSPFDSAGRRHTSFSSVMVSPELDDDIEIEIEEKDIRI
EEECCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCHHHEEECCCCCCCEEEEEECCCEEE
DYYRASGAGGQHVNKTESAVRITHFPTGIVVQCQNDRSQHKNKATAFKMLKSRLYELELM
EEEEECCCCCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
KQQDSANTGEKSEIGWGHQIRSYVLFPYQQVKDNRSGEAFSQVDNILDGDIKKMIEGVLI
HHHCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHE
SLKAE
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA