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The map label for this gene is ptmA [H]

Identifier: 57238378

GI number: 57238378

Start: 1411670

End: 1412440

Strand: Direct

Name: ptmA [H]

Synonym: CJE1521

Alternate gene names: 57238378

Gene position: 1411670-1412440 (Clockwise)

Preceding gene: 57238377

Following gene: 57238379

Centisome position: 79.4

GC content: 34.11

Gene sequence:

>771_bases
ATGCTTGAAAATAAAATCATCTTTGTAGCAGGAGCTTGTGGGCGTATAGGTAAAGCGCTTTGTCAAAAAATACTTCTTAG
CAAAGGTATCCCTATACTCGCAGATATCAACAAAGAACGCTTAAATAAATTGCAAGAAAATTTAGAAACAAATTTTAAAA
CAAAACTTTTAAGCTTAGAACTTGATATCACTAAACAAGAAAGTTTACAAATTGCCCTTCAAAAAAGTCAAGAAAGATAC
GGCAAAATCGATGCTTTTGTTAACTCAAGCTATCCTTTTGGCAAAGATTGGGGTAAAACGCCTTATTATGAACTCAAATA
CGAACAAATTTGTGAAAGTTTAAATTTACATTTAGCAGGCTTTATGCTAGCCGCTCAAGAATTTGTGAAATTTTTTAAAC
AACAAGGCCATGGTAATATCATCAATCTTAGCTCCATCATGGGAGTTTATGCGCCAAAATTTGAAAACTATGAAGGTACT
TCCATGCAAAGCTCTTTAGAATATAGCGTAATAAAAGCAGGGATTAATCACATGAGCTCTTGGCTAGCTAAAGAGCTTTT
TAATCAAAACATACGCGTTAATACCCTAGCAAGCGGGGGAATTTTAGACAATCAAAACGAACTTTTTTTAAAAGCTTATA
GAAAATGTTGTGCAAGCAAGGGTATGTTAGATGCTGATGATATATGTGGAACTTTGGTGTTTTTACTTAGCGATGAGAGT
AAATTTATCACAGGGCAAACCTTAGTAGTAGATGATGGATGGGGCTTATGA

Upstream 100 bases:

>100_bases
TAATGGATGAAAGCACAGCTTTTGATGTAGATAGCGAGCTTGATTTTAAAATCGTAGAGTTTTTAATCTCTTTAAAAAAT
TTATCGCCAAAGGATTTTTA

Downstream 100 bases:

>100_bases
TGACCTTCACCCCTACCCAAAAAGAACTCTTTAACAAAAACATTGAGGCTTTAAGTAATATTCTTTTAAAAGAAAGTTTA
AAAGAAATTAAATCAAGTAA

Product: flagellin modification protein A

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MLENKIIFVAGACGRIGKALCQKILLSKGIPILADINKERLNKLQENLETNFKTKLLSLELDITKQESLQIALQKSQERY
GKIDAFVNSSYPFGKDWGKTPYYELKYEQICESLNLHLAGFMLAAQEFVKFFKQQGHGNIINLSSIMGVYAPKFENYEGT
SMQSSLEYSVIKAGINHMSSWLAKELFNQNIRVNTLASGGILDNQNELFLKAYRKCCASKGMLDADDICGTLVFLLSDES
KFITGQTLVVDDGWGL

Sequences:

>Translated_256_residues
MLENKIIFVAGACGRIGKALCQKILLSKGIPILADINKERLNKLQENLETNFKTKLLSLELDITKQESLQIALQKSQERY
GKIDAFVNSSYPFGKDWGKTPYYELKYEQICESLNLHLAGFMLAAQEFVKFFKQQGHGNIINLSSIMGVYAPKFENYEGT
SMQSSLEYSVIKAGINHMSSWLAKELFNQNIRVNTLASGGILDNQNELFLKAYRKCCASKGMLDADDICGTLVFLLSDES
KFITGQTLVVDDGWGL
>Mature_256_residues
MLENKIIFVAGACGRIGKALCQKILLSKGIPILADINKERLNKLQENLETNFKTKLLSLELDITKQESLQIALQKSQERY
GKIDAFVNSSYPFGKDWGKTPYYELKYEQICESLNLHLAGFMLAAQEFVKFFKQQGHGNIINLSSIMGVYAPKFENYEGT
SMQSSLEYSVIKAGINHMSSWLAKELFNQNIRVNTLASGGILDNQNELFLKAYRKCCASKGMLDADDICGTLVFLLSDES
KFITGQTLVVDDGWGL

Specific function: Required for biosynthesis of LAH modification in the post-translational modification of Campylobacter coli flagellin [H]

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI40254992, Length=260, Percent_Identity=28.4615384615385, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1787335, Length=256, Percent_Identity=26.5625, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789208, Length=269, Percent_Identity=25.2788104089219, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788459, Length=203, Percent_Identity=24.6305418719212, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17555706, Length=261, Percent_Identity=26.8199233716475, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25147288, Length=262, Percent_Identity=27.0992366412214, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17560676, Length=267, Percent_Identity=22.8464419475655, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323882, Length=259, Percent_Identity=25.8687258687259, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324126, Length=267, Percent_Identity=25.0936329588015, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28571526, Length=265, Percent_Identity=26.7924528301887, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24644339, Length=263, Percent_Identity=27.3764258555133, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21357041, Length=267, Percent_Identity=26.5917602996255, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI23397609, Length=263, Percent_Identity=24.3346007604563, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24639444, Length=261, Percent_Identity=24.1379310344828, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 28707; Mature: 28707

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENKIIFVAGACGRIGKALCQKILLSKGIPILADINKERLNKLQENLETNFKTKLLSLE
CCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCHHHEEEEEE
LDITKQESLQIALQKSQERYGKIDAFVNSSYPFGKDWGKTPYYELKYEQICESLNLHLAG
ECCCHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCCCCCCEECCHHHHHHHHCHHHHH
FMLAAQEFVKFFKQQGHGNIINLSSIMGVYAPKFENYEGTSMQSSLEYSVIKAGINHMSS
HHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
WLAKELFNQNIRVNTLASGGILDNQNELFLKAYRKCCASKGMLDADDICGTLVFLLSDES
HHHHHHHCCCCEEEEEECCCEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCC
KFITGQTLVVDDGWGL
CEEECCEEEEECCCCC
>Mature Secondary Structure
MLENKIIFVAGACGRIGKALCQKILLSKGIPILADINKERLNKLQENLETNFKTKLLSLE
CCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCHHHEEEEEE
LDITKQESLQIALQKSQERYGKIDAFVNSSYPFGKDWGKTPYYELKYEQICESLNLHLAG
ECCCHHHHHHHHHHHHHHHHCCHHHEECCCCCCCCCCCCCCCEECCHHHHHHHHCHHHHH
FMLAAQEFVKFFKQQGHGNIINLSSIMGVYAPKFENYEGTSMQSSLEYSVIKAGINHMSS
HHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
WLAKELFNQNIRVNTLASGGILDNQNELFLKAYRKCCASKGMLDADDICGTLVFLLSDES
HHHHHHHCCCCEEEEEECCCEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHEECCCC
KFITGQTLVVDDGWGL
CEEECCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8825781 [H]