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The map label for this gene is lgrA [H]

Identifier: 57238356

GI number: 57238356

Start: 1393364

End: 1394083

Strand: Direct

Name: lgrA [H]

Synonym: CJE1498

Alternate gene names: 57238356

Gene position: 1393364-1394083 (Clockwise)

Preceding gene: 57238355

Following gene: 57238357

Centisome position: 78.37

GC content: 26.53

Gene sequence:

>720_bases
ATGACTTACTCATATATTTACATCATAGGCACAGGCAAAGTAGCCAAAGAATGCCAAAAAATAGCTAATGATTTTTTCAG
ACAAGAAGTAAATTTTGTAAAAAATATAGAAAATTTAGATGATTTCTTTAAAAATCTTAAAAATTGCCTTATTATTAGTG
CTAATAATTTTTATATTTTCAAAAAAGAATGTATTCAAAAAAATACTATTATTAATTACCACAACGCATTATTGCCTTTT
CACAGAGGATGTAATGCACATATTTGGAGTATATGGGAAAACGATAAAAAAACAGGTATTACTTGGCATATGGTTAAAGA
AAGTATAGATACAGGTGATATTTTAGTTCAAAAAGAAATAAAACTTGATAATAATTGTACTGCTTTAAGTCTTTTAAACG
CTCAACACAAACTTGCTCTAACTTTATTTAGAGAAGCTTTAGAAATTTTAAAAAATAAAACTTTTAAAATGCAAATTTCA
GGGGGGGGGTATCACAAAAAATTATCTTTACCAAATAATGGTTATTTAGATCTTACTTGGAATAAAGAAAAAATTTCTCG
CTTTTTAAGAGCTATGGACTGCGGTGCCTTAAGCGGAGTTCCTAAAGCTAGATTAGAAATATTAGGTGAAGAAAGAGAAA
TTTTATTTTATGAAATCAATGAGCTTGACTTAATTTTAAATTTAAGCGATAATGTCATTTTAAAAATCACAAAGGAATAA

Upstream 100 bases:

>100_bases
AACTTTTTGTAGAAGAAAAAAATGAAAAAGCTATCTATTTTTATCAAAGGGCAGGTTTTTGTTTCAATAATATTAATTTA
AAATTTTATAGGAATTTTTA

Downstream 100 bases:

>100_bases
AATGCAAGAAATCAAACAATTTTTTATCAACATAGATAGAGCCGATATTGATGAAAGTATGAAAAATTTAGTAAATGAAG
ATCTTATTGATAGTATAGAT

Product: formyl transferase

Products: tetrahydrofolate; N-formylmethionyl-tRNA [C]

Alternate protein names: ATP-dependent valine/leucine adenylase; Val/LeuA; Valine/leucine activase; ATP-dependent glycine adenylase; GlyA; Glycine activase [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MTYSYIYIIGTGKVAKECQKIANDFFRQEVNFVKNIENLDDFFKNLKNCLIISANNFYIFKKECIQKNTIINYHNALLPF
HRGCNAHIWSIWENDKKTGITWHMVKESIDTGDILVQKEIKLDNNCTALSLLNAQHKLALTLFREALEILKNKTFKMQIS
GGGYHKKLSLPNNGYLDLTWNKEKISRFLRAMDCGALSGVPKARLEILGEEREILFYEINELDLILNLSDNVILKITKE

Sequences:

>Translated_239_residues
MTYSYIYIIGTGKVAKECQKIANDFFRQEVNFVKNIENLDDFFKNLKNCLIISANNFYIFKKECIQKNTIINYHNALLPF
HRGCNAHIWSIWENDKKTGITWHMVKESIDTGDILVQKEIKLDNNCTALSLLNAQHKLALTLFREALEILKNKTFKMQIS
GGGYHKKLSLPNNGYLDLTWNKEKISRFLRAMDCGALSGVPKARLEILGEEREILFYEINELDLILNLSDNVILKITKE
>Mature_238_residues
TYSYIYIIGTGKVAKECQKIANDFFRQEVNFVKNIENLDDFFKNLKNCLIISANNFYIFKKECIQKNTIINYHNALLPFH
RGCNAHIWSIWENDKKTGITWHMVKESIDTGDILVQKEIKLDNNCTALSLLNAQHKLALTLFREALEILKNKTFKMQISG
GGYHKKLSLPNNGYLDLTWNKEKISRFLRAMDCGALSGVPKARLEILGEEREILFYEINELDLILNLSDNVILKITKE

Specific function: Activates valine (or leucine, but much less frequently), and then glycine and catalyzes the formation of the peptide bond in the first step of peptide synthesis. This enzyme may also play a role in N-formylation of the first amino acid residue in the synt

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 acyl carrier domains [H]

Homologues:

None

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR002376
- InterPro:   IPR010060
- InterPro:   IPR006163
- InterPro:   IPR006162 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00551 Formyl_trans_N; PF00550 PP-binding [H]

EC number: 2.1.2.9 [C]

Molecular weight: Translated: 27683; Mature: 27551

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYSYIYIIGTGKVAKECQKIANDFFRQEVNFVKNIENLDDFFKNLKNCLIISANNFYIF
CCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEE
KKECIQKNTIINYHNALLPFHRGCNAHIWSIWENDKKTGITWHMVKESIDTGDILVQKEI
EHHHHCCCCEEEECCCCCHHCCCCCCEEEEEEECCCCCCEEEEEEHHCCCCCCEEEEEEE
KLDNNCTALSLLNAQHKLALTLFREALEILKNKTFKMQISGGGYHKKLSLPNNGYLDLTW
EECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCCCEEEEEE
NKEKISRFLRAMDCGALSGVPKARLEILGEEREILFYEINELDLILNLSDNVILKITKE
CHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEEEEECCEEEEEECCCCEEEEEECC
>Mature Secondary Structure 
TYSYIYIIGTGKVAKECQKIANDFFRQEVNFVKNIENLDDFFKNLKNCLIISANNFYIF
CEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCEEEE
KKECIQKNTIINYHNALLPFHRGCNAHIWSIWENDKKTGITWHMVKESIDTGDILVQKEI
EHHHHCCCCEEEECCCCCHHCCCCCCEEEEEEECCCCCCEEEEEEHHCCCCCCEEEEEEE
KLDNNCTALSLLNAQHKLALTLFREALEILKNKTFKMQISGGGYHKKLSLPNNGYLDLTW
EECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEECCCCCEEEEEE
NKEKISRFLRAMDCGALSGVPKARLEILGEEREILFYEINELDLILNLSDNVILKITKE
CHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCEEEEEEECCEEEEEECCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+; K+; Mg2+; Na+; NH4+ [C]

Kcat value (1/min): 1200 [C]

Specific activity: 1.14

Km value (mM): 0.013 {10-formyltetrahydrofolate}} 0.0006 {methionyl-tRNAfMet}} 0.00035 {methionyl-tRNAfMet}} 0.0013 {phenylalanyl-tRNAfMet}} 0.0014 {valyl-tRNAfMet}} [C]

Substrates: Formyltetrahydrofolate; L-methionyl-tRNA; H2O [C]

Specific reaction: Formyltetrahydrofolate + L-methionyl-tRNA + H2O =tetrahydrofolate + N-formylmethionyl-tRNA [C]

General reaction: Formyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA