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The map label for this gene is alaS [H]

Identifier: 57238291

GI number: 57238291

Start: 548051

End: 550579

Strand: Direct

Name: alaS [H]

Synonym: CJE0613

Alternate gene names: 57238291

Gene position: 548051-550579 (Clockwise)

Preceding gene: 57238295

Following gene: 57238290

Centisome position: 30.83

GC content: 34.12

Gene sequence:

>2529_bases
ATGGATATTAGAAAAGCTTATTTGGATTTTTTTGCATCAAAAGGACATGAGATAACCCCGTCAAGTCCTTTAGTTCCTGA
TGATGCAACTTTACTTTTTACCAATGCGGGCATGGTGCCTTTTAAAAGTATATTTACAGGAGAAATTCCACGCCCAAACC
CTCCACGCAAAACAAGCTGTCAAACTTGTATAAGAGCGGGTGGAAAGCACAATGACTTAGATAATGTAGGATATACCGCA
CGCCATCATACTTTTTTTGAAATGCTTGGGAATTTTAGTTTTGGGGATTATTTTAAAGAACAAGCCATAGCTTATGCTTG
GGAATTTGTAACTGAAGTTTTAAAACTACCTAAAGATAGACTTTATGTAACCGTGCATGAAAACGATGATGAAGCTTTTA
ATCTATGGCAAAAACACATTCAAAAAGAAAGAATTTACAAATTTGGCGATAAAGATAACTTCTGGCAAATGGGCGATACT
GGTCCTTGTGGTCCTTGTAGTGAGATTTTTTATGACCAAGGACAAGAGCATTTTAACTCAAGCGAAGATTATATGGGCGG
AGATGGAGATAGATTTTTAGAAATCTGGAATCTTGTTTTCATGCAATACGAAAGAAGTGCTGATGGAGTGCTTAGCCCAC
TGCCAAAGCCAAGCATTGATACAGGAATGGGGCTTGAAAGGGTAACAGCGATTAAAGAAGGTAAATTTAGCAATTTTGAT
AGCTCGCTTTTTATGCCAATCATCAATGAAATTTCAAAACTTTGTAATAAAACTTATGTTTATGAAAGCGGAGCAAGTTT
TAGAGTCATAGCTGATCATATAAGATCAAGTGTATTTTTGCTTGCACAAGGGGTAAGTTTTGATAAAGAAGGTAGAGGTT
ATGTTTTGCGTCGTATTTTGCGTCGTGCTTTAAGACATGGATATTTGCTAGGTTTTAAACAAGCTTTTATGTATAAACTA
GTTGATATTGTGTGCGATTTAATGGGCGGACATTATACCTATCTTAATGAGAAAAAAGACTTTATAAAAGAACAAATTCG
CCTTGAAGAAGAAAGATTTTTAAGCACTATAGAAAATGGGATTGAAATTTTTAATGAAGAGCTTAAAAACACTAAAGAAA
TTTTTAGCGGAGAAGTAGCCTTTAAGCTTTATGATACTTATGGTTTTCCGCTTGATTTAACGGCTGATATGCTAAGAGAA
AAAAATCTTAAAGTTGATGAAGAAAAATTTGAACTTTTCATGAATGAGCAAAAAGCACGCGCTAAGGCTTCTTGGAAAGG
AAGTGGAGATAAAACTGCAAGCGGAGATTTTAAAAACTTACTTGAAAAATTTGGAGAAAATCATTTCGTAGGTTATGAAA
AAGCAGAATGCGAAAGTAAAATTTTAGCCCTTTTAGATGAAGATTTTAAAGAAGTTTCCACCCTTAAAGATGCGGGTTGG
GTGATGCTTGAAAACACACCTTTTTATGCAACAAGTGGGGGGCAAAGTGCTGATAGTGGATTTATAGCTAAGCGTGAAGT
TTTAGATACGCAAAAGTTTTTTAATCTCAATCTTAGCTTTATAAAAGCAGGCGAAGAACTAAAAGTTAACGATATAGTTC
ATGCAAGAATCGATACAGAAAAAAGAGAGCAAATCGCACGCCACCACTCAGCCACTCATCTTTTACATCATGCTTTAAGA
GAAATTTTAGGTTCTCATGTAAGCCAAGCAGGATCTTTGGTTGAAAGCAATAAACTAAGATTTGATTTTACTCATCATAA
AGCTTTAAACAAAGAAGAGCTAGAAAGCATTGAAAAAAGAGTCAATAAAATGATTATAAACTCAAGCGAAGCTATCTTAG
AAAATATGCCTTTAGAAGAAGCTAAAAAAAGCGGTGCTATAGCATTGTTTAATGAAAAATACCAAGGCAATGTGCGTGTT
TTAACTCTAGGTGAAAGCAAAGAACTTTGCGGGGGAACTCATGTTAAAAATACTGCTCAAATAGGAAGTTTTTACATAGT
TAAAGAAAGTGGTGTGAGTGCTGGAGTTAGACGTATTGAAGCTGTGGTTTCTAAAGCGGCTTTAGAATTTGTAAAAAATC
AACTCGAAGAACTTTCTAAAGTAAAAGATGAACTTAAAAATAATGATATTTTAAGCGGGATTAAAAAGCTTAAAAATGAA
ATTTTAAGTTTAAAAAATGAGCTTAAAAATTCAAGTAAAACCGAGCTTGATTCTAAAAATATCCAAGGTGTTGAAATTTG
CGTAAAACGTATAGATAATGGCGATATTAAAGCTATGATTGATGATTTTAAAAACAAATTTGCTAAAGCGGTGATTTTAC
TTATCCAAGTAAAAGATGAAAAAATCACTTTAGCAGCTGGCGTTAAAGATGTGCCTTTAAAAGCAGGAGCTTTGGTTAAA
GAAGCGGCGCAAATTTTAGGTGGAAATGGTGGCGGAAGAGATGATTTTGCTACTGCAGGTGGAAAAGATTTAAGCAAAAT
CAATGAAGCTTTAAAACAAAGCTTAGAAACGATAGAAAAAGCACTTTAA

Upstream 100 bases:

>100_bases
AAATAAAATTAAATAAAAATGTTTATTGTATTTTAGTTTTACTTAATTTAAAGTAATTTTGGTATGATATGTTTTTATAA
AAATTTCTTTAGGAAAATTC

Downstream 100 bases:

>100_bases
TGTTAATTCTTGCTTCAAGTTCCATTTCAAGAGCTAACTTGCTTAAAACCGCAAAGATTGATTTTAGGCAAGTTAGTTTT
GATTATGATGAAAATTTAAA

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 842; Mature: 842

Protein sequence:

>842_residues
MDIRKAYLDFFASKGHEITPSSPLVPDDATLLFTNAGMVPFKSIFTGEIPRPNPPRKTSCQTCIRAGGKHNDLDNVGYTA
RHHTFFEMLGNFSFGDYFKEQAIAYAWEFVTEVLKLPKDRLYVTVHENDDEAFNLWQKHIQKERIYKFGDKDNFWQMGDT
GPCGPCSEIFYDQGQEHFNSSEDYMGGDGDRFLEIWNLVFMQYERSADGVLSPLPKPSIDTGMGLERVTAIKEGKFSNFD
SSLFMPIINEISKLCNKTYVYESGASFRVIADHIRSSVFLLAQGVSFDKEGRGYVLRRILRRALRHGYLLGFKQAFMYKL
VDIVCDLMGGHYTYLNEKKDFIKEQIRLEEERFLSTIENGIEIFNEELKNTKEIFSGEVAFKLYDTYGFPLDLTADMLRE
KNLKVDEEKFELFMNEQKARAKASWKGSGDKTASGDFKNLLEKFGENHFVGYEKAECESKILALLDEDFKEVSTLKDAGW
VMLENTPFYATSGGQSADSGFIAKREVLDTQKFFNLNLSFIKAGEELKVNDIVHARIDTEKREQIARHHSATHLLHHALR
EILGSHVSQAGSLVESNKLRFDFTHHKALNKEELESIEKRVNKMIINSSEAILENMPLEEAKKSGAIALFNEKYQGNVRV
LTLGESKELCGGTHVKNTAQIGSFYIVKESGVSAGVRRIEAVVSKAALEFVKNQLEELSKVKDELKNNDILSGIKKLKNE
ILSLKNELKNSSKTELDSKNIQGVEICVKRIDNGDIKAMIDDFKNKFAKAVILLIQVKDEKITLAAGVKDVPLKAGALVK
EAAQILGGNGGGRDDFATAGGKDLSKINEALKQSLETIEKAL

Sequences:

>Translated_842_residues
MDIRKAYLDFFASKGHEITPSSPLVPDDATLLFTNAGMVPFKSIFTGEIPRPNPPRKTSCQTCIRAGGKHNDLDNVGYTA
RHHTFFEMLGNFSFGDYFKEQAIAYAWEFVTEVLKLPKDRLYVTVHENDDEAFNLWQKHIQKERIYKFGDKDNFWQMGDT
GPCGPCSEIFYDQGQEHFNSSEDYMGGDGDRFLEIWNLVFMQYERSADGVLSPLPKPSIDTGMGLERVTAIKEGKFSNFD
SSLFMPIINEISKLCNKTYVYESGASFRVIADHIRSSVFLLAQGVSFDKEGRGYVLRRILRRALRHGYLLGFKQAFMYKL
VDIVCDLMGGHYTYLNEKKDFIKEQIRLEEERFLSTIENGIEIFNEELKNTKEIFSGEVAFKLYDTYGFPLDLTADMLRE
KNLKVDEEKFELFMNEQKARAKASWKGSGDKTASGDFKNLLEKFGENHFVGYEKAECESKILALLDEDFKEVSTLKDAGW
VMLENTPFYATSGGQSADSGFIAKREVLDTQKFFNLNLSFIKAGEELKVNDIVHARIDTEKREQIARHHSATHLLHHALR
EILGSHVSQAGSLVESNKLRFDFTHHKALNKEELESIEKRVNKMIINSSEAILENMPLEEAKKSGAIALFNEKYQGNVRV
LTLGESKELCGGTHVKNTAQIGSFYIVKESGVSAGVRRIEAVVSKAALEFVKNQLEELSKVKDELKNNDILSGIKKLKNE
ILSLKNELKNSSKTELDSKNIQGVEICVKRIDNGDIKAMIDDFKNKFAKAVILLIQVKDEKITLAAGVKDVPLKAGALVK
EAAQILGGNGGGRDDFATAGGKDLSKINEALKQSLETIEKAL
>Mature_842_residues
MDIRKAYLDFFASKGHEITPSSPLVPDDATLLFTNAGMVPFKSIFTGEIPRPNPPRKTSCQTCIRAGGKHNDLDNVGYTA
RHHTFFEMLGNFSFGDYFKEQAIAYAWEFVTEVLKLPKDRLYVTVHENDDEAFNLWQKHIQKERIYKFGDKDNFWQMGDT
GPCGPCSEIFYDQGQEHFNSSEDYMGGDGDRFLEIWNLVFMQYERSADGVLSPLPKPSIDTGMGLERVTAIKEGKFSNFD
SSLFMPIINEISKLCNKTYVYESGASFRVIADHIRSSVFLLAQGVSFDKEGRGYVLRRILRRALRHGYLLGFKQAFMYKL
VDIVCDLMGGHYTYLNEKKDFIKEQIRLEEERFLSTIENGIEIFNEELKNTKEIFSGEVAFKLYDTYGFPLDLTADMLRE
KNLKVDEEKFELFMNEQKARAKASWKGSGDKTASGDFKNLLEKFGENHFVGYEKAECESKILALLDEDFKEVSTLKDAGW
VMLENTPFYATSGGQSADSGFIAKREVLDTQKFFNLNLSFIKAGEELKVNDIVHARIDTEKREQIARHHSATHLLHHALR
EILGSHVSQAGSLVESNKLRFDFTHHKALNKEELESIEKRVNKMIINSSEAILENMPLEEAKKSGAIALFNEKYQGNVRV
LTLGESKELCGGTHVKNTAQIGSFYIVKESGVSAGVRRIEAVVSKAALEFVKNQLEELSKVKDELKNNDILSGIKKLKNE
ILSLKNELKNSSKTELDSKNIQGVEICVKRIDNGDIKAMIDDFKNKFAKAVILLIQVKDEKITLAAGVKDVPLKAGALVK
EAAQILGGNGGGRDDFATAGGKDLSKINEALKQSLETIEKAL

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=958, Percent_Identity=35.80375782881, Blast_Score=491, Evalue=1e-138,
Organism=Homo sapiens, GI38569417, Length=758, Percent_Identity=34.5646437994723, Blast_Score=409, Evalue=1e-114,
Organism=Escherichia coli, GI1789048, Length=862, Percent_Identity=47.9118329466357, Blast_Score=753, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=977, Percent_Identity=34.6980552712385, Blast_Score=484, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI17536681, Length=745, Percent_Identity=35.3020134228188, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324911, Length=760, Percent_Identity=39.3421052631579, Blast_Score=469, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24582809, Length=957, Percent_Identity=35.632183908046, Blast_Score=485, Evalue=1e-137,
Organism=Drosophila melanogaster, GI45552267, Length=957, Percent_Identity=35.632183908046, Blast_Score=485, Evalue=1e-137,
Organism=Drosophila melanogaster, GI24658214, Length=806, Percent_Identity=31.8858560794045, Blast_Score=323, Evalue=3e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 94921; Mature: 94921

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRKAYLDFFASKGHEITPSSPLVPDDATLLFTNAGMVPFKSIFTGEIPRPNPPRKTSC
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCCCCCCHHH
QTCIRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKEQAIAYAWEFVTEVLKLPKDR
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
LYVTVHENDDEAFNLWQKHIQKERIYKFGDKDNFWQMGDTGPCGPCSEIFYDQGQEHFNS
EEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHCCHHHCCC
SEDYMGGDGDRFLEIWNLVFMQYERSADGVLSPLPKPSIDTGMGLERVTAIKEGKFSNFD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
SSLFMPIINEISKLCNKTYVYESGASFRVIADHIRSSVFLLAQGVSFDKEGRGYVLRRIL
CHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
RRALRHGYLLGFKQAFMYKLVDIVCDLMGGHYTYLNEKKDFIKEQIRLEEERFLSTIENG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
IEIFNEELKNTKEIFSGEVAFKLYDTYGFPLDLTADMLREKNLKVDEEKFELFMNEQKAR
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
AKASWKGSGDKTASGDFKNLLEKFGENHFVGYEKAECESKILALLDEDFKEVSTLKDAGW
HHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCE
VMLENTPFYATSGGQSADSGFIAKREVLDTQKFFNLNLSFIKAGEELKVNDIVHARIDTE
EEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCEEHHHHEEECCCHH
KREQIARHHSATHLLHHALREILGSHVSQAGSLVESNKLRFDFTHHKALNKEELESIEKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCHHHHHHHHHH
VNKMIINSSEAILENMPLEEAKKSGAIALFNEKYQGNVRVLTLGESKELCGGTHVKNTAQ
HHHHHHCCHHHHHHCCCHHHHHCCCCEEEECCCCCCCEEEEEECCCHHHCCCCCCCCHHH
IGSFYIVKESGVSAGVRRIEAVVSKAALEFVKNQLEELSKVKDELKNNDILSGIKKLKNE
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ILSLKNELKNSSKTELDSKNIQGVEICVKRIDNGDIKAMIDDFKNKFAKAVILLIQVKDE
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEEECCC
KITLAAGVKDVPLKAGALVKEAAQILGGNGGGRDDFATAGGKDLSKINEALKQSLETIEK
EEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AL
CC
>Mature Secondary Structure
MDIRKAYLDFFASKGHEITPSSPLVPDDATLLFTNAGMVPFKSIFTGEIPRPNPPRKTSC
CCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCCCCCCCCCCHHH
QTCIRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYFKEQAIAYAWEFVTEVLKLPKDR
HHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
LYVTVHENDDEAFNLWQKHIQKERIYKFGDKDNFWQMGDTGPCGPCSEIFYDQGQEHFNS
EEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHCCHHHCCC
SEDYMGGDGDRFLEIWNLVFMQYERSADGVLSPLPKPSIDTGMGLERVTAIKEGKFSNFD
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCC
SSLFMPIINEISKLCNKTYVYESGASFRVIADHIRSSVFLLAQGVSFDKEGRGYVLRRIL
CHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHH
RRALRHGYLLGFKQAFMYKLVDIVCDLMGGHYTYLNEKKDFIKEQIRLEEERFLSTIENG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
IEIFNEELKNTKEIFSGEVAFKLYDTYGFPLDLTADMLREKNLKVDEEKFELFMNEQKAR
HHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
AKASWKGSGDKTASGDFKNLLEKFGENHFVGYEKAECESKILALLDEDFKEVSTLKDAGW
HHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCE
VMLENTPFYATSGGQSADSGFIAKREVLDTQKFFNLNLSFIKAGEELKVNDIVHARIDTE
EEEECCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCEEHHHHEEECCCHH
KREQIARHHSATHLLHHALREILGSHVSQAGSLVESNKLRFDFTHHKALNKEELESIEKR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHCCCHHHHHHHHHH
VNKMIINSSEAILENMPLEEAKKSGAIALFNEKYQGNVRVLTLGESKELCGGTHVKNTAQ
HHHHHHCCHHHHHHCCCHHHHHCCCCEEEECCCCCCCEEEEEECCCHHHCCCCCCCCHHH
IGSFYIVKESGVSAGVRRIEAVVSKAALEFVKNQLEELSKVKDELKNNDILSGIKKLKNE
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ILSLKNELKNSSKTELDSKNIQGVEICVKRIDNGDIKAMIDDFKNKFAKAVILLIQVKDE
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEEEECCC
KITLAAGVKDVPLKAGALVKEAAQILGGNGGGRDDFATAGGKDLSKINEALKQSLETIEK
EEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
AL
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA