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The map label for this gene is proS [H]

Identifier: 57238257

GI number: 57238257

Start: 583652

End: 585361

Strand: Direct

Name: proS [H]

Synonym: CJE0647

Alternate gene names: 57238257

Gene position: 583652-585361 (Clockwise)

Preceding gene: 57238258

Following gene: 57238256

Centisome position: 32.83

GC content: 32.22

Gene sequence:

>1710_bases
ATGATGAGATTTACTAAATTTTATGCACCAAGTTTAAAAGAAGCGCCAAAAGATGCGAGTTTACCTAGTCATATTTTTTT
AACGCGTGCGGGTTTTGTAGAACAAATTGGTAGCGGACTTTATAATTTCTTGCCTTTGGGAAAAAGAGTTTTAGACAAGA
TTAAAAATATAGTTAAAGAAGAAATGGATAAGGCTGGAGCACAAGAAGTAAATCTTAGTTTTATCACTCCTGCAAGTTTG
TGGCAAGAAAGTGGGCGTTACAATGTTTTTGGAAAAGAACTTTTGCGTTTTAAAGACAGAAAAGAAAATGAATTTGTTTT
AGGTCCAACACATGAAGAAGCTATGCTTTCTTTGGTAAAAAACAAAATCACAAGCTATAAACAACTTCCTTTGCATTTAT
ATCAAATTGGACTTAAATTTCGTGATGAAGCAAGGCCTAGATTTGGGCTTTTAAGATGTCGTGAATTTCTAATGAAAGAT
GGATATAGTTTTCATGCTAATGAAGAAGATTTAGGGCGTGAGTTTGAGCTTATGTATAAAACTTATTCTCAAATTTTACA
AAGAATGGGGCTTGATTTTAGAGCAGTTGAAGCTGATAGTGGGGCTATTGGTGGAAGTGGCTCTAAGGAATTTATGGTTT
TAGCAAAAAATGGGGAAGATGATATTTTAATTTGTGAAAATTGTGATTATGCGGCTAATGTTGAAGCTGCAAAAAGAGCT
AAAAAAACTTGTCAAGATGAGCGTCCTGAAGCAAATTATGCAAGTAAATTTCACACTCCAAATATTAAAACTATAGATTC
TTTAGCACAATTTTTTAAAATCAATGCTTTTTATACGATTAAAGCAGTGGTAAAAAAAGCTATTTATGAAAATGAAAGCA
AATTAGTTGTGTTTTTTATAAGAGGAAGTGATGATTTGCAAGAGGTTAAGGCTCAAAATGCTTGCAGTGCCCTTGAACTA
GTCGATGCGAGCGAAGAAGAATTGGAAAAAGCTGGCTTAGTAGCTGGTTTTATAGGTTTTGTGGGATTAAAAGATATAGA
TTTTTATATAGATTTTGAGCTTGAAAACGAAAAGCAAATGATTATGGGTGCGAATGAAAAAGATTATCATTTAATCGGTA
TTGATGTTGTAAATTTAAATAAAGATCGTTTTAAAGATTTGATTGAAGTTAAAGAAGGGGATTGCTGTGTTAAATGTGGA
GCAAAATTAAAACAAAGTAAAGGTATAGAAGTAGGGCATATTTTTAAGCTAGGACAAAAATATTCCAAAGCGATGAATGC
AAATTTCTTAGATGAAAATGGCAAAAGTCAGCCTTTTTACATGGGATGTTATGGCATAGGTGTTTCACGCTTGTTGGCTG
TAGCTACTGAAGCAAGTCATGATGAAAAAGGATGTATTTGGAATAAAACTTTAGCTCCTTTTGTGCTTGAAATTATTGTT
TCAAATCTCAAAGATGAAAAGGCTTTAGAATTTGCTAATAAGCTTTATGAGGATTTAACTAATCTTGGATTAGAGGTATT
GCTTGATGATAGAAATGAGCGTTTTGGGGTTAAAATGAATGATTTTGAACTTATGGGCTTTCCTTATGCGTTAGTTGTTG
GTAAAGGATTAGAAAAGAATGAAATAGAGCTTATCCAAAGAGAAGGTTTGATAAAAGAACTTATAAAAACTGATGAACTT
ATGGAAATTCTTAAAAAGAAAGTGTTATGA

Upstream 100 bases:

>100_bases
CAAAGCGATACAGTGATTAATGCTATGCGTTATGTTTTTGATTTGCAAAATAATCTTGAAGGTTTAAATCAATATAAATG
TGAATTTGATATGGAGAATA

Downstream 100 bases:

>100_bases
ATTATAAATTAAGTTTAAGCCCTTTATTTGTACTTGAAATTATTGCAAGTATACTTTTTATAGTGTTTTTTGGTTTTGGT
AATTTTTTATTTTTCATACT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MMRFTKFYAPSLKEAPKDASLPSHIFLTRAGFVEQIGSGLYNFLPLGKRVLDKIKNIVKEEMDKAGAQEVNLSFITPASL
WQESGRYNVFGKELLRFKDRKENEFVLGPTHEEAMLSLVKNKITSYKQLPLHLYQIGLKFRDEARPRFGLLRCREFLMKD
GYSFHANEEDLGREFELMYKTYSQILQRMGLDFRAVEADSGAIGGSGSKEFMVLAKNGEDDILICENCDYAANVEAAKRA
KKTCQDERPEANYASKFHTPNIKTIDSLAQFFKINAFYTIKAVVKKAIYENESKLVVFFIRGSDDLQEVKAQNACSALEL
VDASEEELEKAGLVAGFIGFVGLKDIDFYIDFELENEKQMIMGANEKDYHLIGIDVVNLNKDRFKDLIEVKEGDCCVKCG
AKLKQSKGIEVGHIFKLGQKYSKAMNANFLDENGKSQPFYMGCYGIGVSRLLAVATEASHDEKGCIWNKTLAPFVLEIIV
SNLKDEKALEFANKLYEDLTNLGLEVLLDDRNERFGVKMNDFELMGFPYALVVGKGLEKNEIELIQREGLIKELIKTDEL
MEILKKKVL

Sequences:

>Translated_569_residues
MMRFTKFYAPSLKEAPKDASLPSHIFLTRAGFVEQIGSGLYNFLPLGKRVLDKIKNIVKEEMDKAGAQEVNLSFITPASL
WQESGRYNVFGKELLRFKDRKENEFVLGPTHEEAMLSLVKNKITSYKQLPLHLYQIGLKFRDEARPRFGLLRCREFLMKD
GYSFHANEEDLGREFELMYKTYSQILQRMGLDFRAVEADSGAIGGSGSKEFMVLAKNGEDDILICENCDYAANVEAAKRA
KKTCQDERPEANYASKFHTPNIKTIDSLAQFFKINAFYTIKAVVKKAIYENESKLVVFFIRGSDDLQEVKAQNACSALEL
VDASEEELEKAGLVAGFIGFVGLKDIDFYIDFELENEKQMIMGANEKDYHLIGIDVVNLNKDRFKDLIEVKEGDCCVKCG
AKLKQSKGIEVGHIFKLGQKYSKAMNANFLDENGKSQPFYMGCYGIGVSRLLAVATEASHDEKGCIWNKTLAPFVLEIIV
SNLKDEKALEFANKLYEDLTNLGLEVLLDDRNERFGVKMNDFELMGFPYALVVGKGLEKNEIELIQREGLIKELIKTDEL
MEILKKKVL
>Mature_569_residues
MMRFTKFYAPSLKEAPKDASLPSHIFLTRAGFVEQIGSGLYNFLPLGKRVLDKIKNIVKEEMDKAGAQEVNLSFITPASL
WQESGRYNVFGKELLRFKDRKENEFVLGPTHEEAMLSLVKNKITSYKQLPLHLYQIGLKFRDEARPRFGLLRCREFLMKD
GYSFHANEEDLGREFELMYKTYSQILQRMGLDFRAVEADSGAIGGSGSKEFMVLAKNGEDDILICENCDYAANVEAAKRA
KKTCQDERPEANYASKFHTPNIKTIDSLAQFFKINAFYTIKAVVKKAIYENESKLVVFFIRGSDDLQEVKAQNACSALEL
VDASEEELEKAGLVAGFIGFVGLKDIDFYIDFELENEKQMIMGANEKDYHLIGIDVVNLNKDRFKDLIEVKEGDCCVKCG
AKLKQSKGIEVGHIFKLGQKYSKAMNANFLDENGKSQPFYMGCYGIGVSRLLAVATEASHDEKGCIWNKTLAPFVLEIIV
SNLKDEKALEFANKLYEDLTNLGLEVLLDDRNERFGVKMNDFELMGFPYALVVGKGLEKNEIELIQREGLIKELIKTDEL
MEILKKKVL

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=235, Percent_Identity=41.7021276595745, Blast_Score=209, Evalue=7e-54,
Organism=Escherichia coli, GI1786392, Length=580, Percent_Identity=45.3448275862069, Blast_Score=491, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI115532348, Length=249, Percent_Identity=37.3493975903614, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI193203271, Length=116, Percent_Identity=32.7586206896552, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=536, Percent_Identity=33.3955223880597, Blast_Score=254, Evalue=2e-68,
Organism=Drosophila melanogaster, GI24656200, Length=252, Percent_Identity=37.3015873015873, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 64525; Mature: 64525

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRFTKFYAPSLKEAPKDASLPSHIFLTRAGFVEQIGSGLYNFLPLGKRVLDKIKNIVKE
CCCCCHHCCCCHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
EMDKAGAQEVNLSFITPASLWQESGRYNVFGKELLRFKDRKENEFVLGPTHEEAMLSLVK
HHHHCCCCEECEEEECHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHH
NKITSYKQLPLHLYQIGLKFRDEARPRFGLLRCREFLMKDGYSFHANEEDLGREFELMYK
HHHHHHHHCCHHHHHHCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
TYSQILQRMGLDFRAVEADSGAIGGSGSKEFMVLAKNGEDDILICENCDYAANVEAAKRA
HHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCHHHHHH
KKTCQDERPEANYASKFHTPNIKTIDSLAQFFKINAFYTIKAVVKKAIYENESKLVVFFI
HHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEEE
RGSDDLQEVKAQNACSALELVDASEEELEKAGLVAGFIGFVGLKDIDFYIDFELENEKQM
ECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCEE
IMGANEKDYHLIGIDVVNLNKDRFKDLIEVKEGDCCVKCGAKLKQSKGIEVGHIFKLGQK
EECCCCCCEEEEEEEEEECCHHHHHHHHHCCCCCCHHHCCCHHHHCCCCCHHHHHHHHHH
YSKAMNANFLDENGKSQPFYMGCYGIGVSRLLAVATEASHDEKGCIWNKTLAPFVLEIIV
HHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHH
SNLKDEKALEFANKLYEDLTNLGLEVLLDDRNERFGVKMNDFELMGFPYALVVGKGLEKN
HHCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEECCEEEECCCEEEEECCCCCCH
EIELIQREGLIKELIKTDELMEILKKKVL
HHHHHHHCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMRFTKFYAPSLKEAPKDASLPSHIFLTRAGFVEQIGSGLYNFLPLGKRVLDKIKNIVKE
CCCCCHHCCCCHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
EMDKAGAQEVNLSFITPASLWQESGRYNVFGKELLRFKDRKENEFVLGPTHEEAMLSLVK
HHHHCCCCEECEEEECHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHH
NKITSYKQLPLHLYQIGLKFRDEARPRFGLLRCREFLMKDGYSFHANEEDLGREFELMYK
HHHHHHHHCCHHHHHHCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
TYSQILQRMGLDFRAVEADSGAIGGSGSKEFMVLAKNGEDDILICENCDYAANVEAAKRA
HHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCCCCHHHHHH
KKTCQDERPEANYASKFHTPNIKTIDSLAQFFKINAFYTIKAVVKKAIYENESKLVVFFI
HHHHHCCCCCCCHHHHCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCEEEEEE
RGSDDLQEVKAQNACSALELVDASEEELEKAGLVAGFIGFVGLKDIDFYIDFELENEKQM
ECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEEEEEECCCCEE
IMGANEKDYHLIGIDVVNLNKDRFKDLIEVKEGDCCVKCGAKLKQSKGIEVGHIFKLGQK
EECCCCCCEEEEEEEEEECCHHHHHHHHHCCCCCCHHHCCCHHHHCCCCCHHHHHHHHHH
YSKAMNANFLDENGKSQPFYMGCYGIGVSRLLAVATEASHDEKGCIWNKTLAPFVLEIIV
HHHHHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHHHHHHHH
SNLKDEKALEFANKLYEDLTNLGLEVLLDDRNERFGVKMNDFELMGFPYALVVGKGLEKN
HHCCHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCEECCEEEECCCEEEEECCCCCCH
EIELIQREGLIKELIKTDELMEILKKKVL
HHHHHHHCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA