Click here to switch to the map view.

The map label for this gene is ilvB [H]

Identifier: 57238230

GI number: 57238230

Start: 611208

End: 612908

Strand: Direct

Name: ilvB [H]

Synonym: CJE0677

Alternate gene names: 57238230

Gene position: 611208-612908 (Clockwise)

Preceding gene: 57238231

Following gene: 57238229

Centisome position: 34.38

GC content: 35.86

Gene sequence:

>1701_bases
ATGAAAGAGTTAAGCGGTTCGGCAATGATTTGCGAGGCTTTAAAAGAAGAAAATGTTAAAATTGTTTTTGGTTATCCTGG
AGGCGCTGCGCTTAATATTTATGATGAAATTTACAATCAAAAGTATTTTAAACATATTTTAGTGCGTCATGAACAAGCGG
CTTTGCATGCTGCTGATGCTTACGCAAGAATGAGCGGAGAAGTGGGCGTAGCTATAGTTACAAGCGGACCAGGTTTTACA
AATACTATTACAGGTCTAGCTACGGCTTATAGCGATTCTATCCCTTTGGTGTTGATTTCAGGGCAGGTGGCAAATTCTCT
TATTGGTACAGATGCTTTTCAAGAAATTGATGCAGTAGGCATTTCAAGACCTTGTGTAAAGCATAATTATTTAGTTACTT
GTATAGAAGAGTTTCCACGCATTTTAAAAGAGGCTTTTTATATAGCAAGAAGTGGTAGACCTGGGCCTGTGCATATAGAT
GTGCCAAAAGACGTAAGTGCAACTTTGGGACTTTGGGAATACTCTAAAGAAATTTCCATGAAGACTTATAAGCCCGTTTA
TAAGGGTAACTCAAAGCAAATTAAAAAATTTGCAGAGCTTTTAAAAGAAGCAAAAAGACCTTTGTTTTACTTAGGTGGAG
GTTGCATCAGTTCTAATGCAAGCGAGCAAATCAGAGAGCTTATAAAATTTACAAAAATTCCAGCAGTTGAAACTTTGATG
GCGCTTGGAACTTTAAGAAGTGATGATGTTTTTAATCTTAAAATGGCAGGCATGCATGGAAGCTATGCTGCTAATATGGC
TTTAAGTGAGTGCGATTTGCTTGTGAGTGTAGGAGCGAGATTTGATGATAGGATTACAGGAAAAACAAGTGAATTTGCTA
AACATGCGACTATAGTTCATGTTGATATTGATCCTAGTTCTATTTCAAAGATTATCAATGCGCATTATCCTATAGTAGGA
GATATTAAAGAAGTTCTTAAAGAGCTTTTAGAAGAGCTTAAAAAGGAAAATTTCAACACAACTTTTAAAGAATGGCACGA
AACTTTAAAGCGTTATAATGAACTTTATCCGCTTTCTTATGAAGATAGCAATGAGATTTTAAAACCGCAATGGGTGATTG
AAGAATGTGCGAAAATGGCTCCAGATGCAAGGATTATTACTGATGTGGGTCAGCATCAAATGTGGGTGGCACAGTTTTAT
CCTTTTAATTATCCAAGACAACTTGCAACAAGCGGAGGGCAAGGAACTATGGGATATTCTTTACCTGCTGCTTTGGGTGC
AAAGCTTGCTGTGGGTGAAGAAGTGGTGATTAATTTCGTAGGCGATGGATCGGTTTTAATGAATATCCAAGAGCTTATGA
CTGCTTATGAGTATGGTATAAAAACTATAAATATTATTTTAAATAATGCCTTTTTAGGTATGGTAAGACAATGGCAAAGT
ATGTTTTATAAAGAGCATTTTTCACAAACTGATTTAAGCACTCAGCCTGATTTTATCAAAATAGCTCAAGGTTTTGGTTG
TGAAGGTTATGAAATTTCAAACAAAGAAGAATTCATTCAAGCTTTTAGCCAAGCTTTAAAAAGTGATAAAACCTCTCTTT
TAAATGTAAAAATTGATCGCTTTGAAGATGTTTTACCTATGGTGCCTGCAGGAGGTGCGATTTATAATATGATTTTACCA
AAGGCAAAGGATAGACAATGA

Upstream 100 bases:

>100_bases
TAATGAAAGAAGCTAAAATAAAATTAGGTGCGAGTGTTGATGGCAAAAGACTTAATTTAGCACTTAAAGAGCTTTTACAA
TAATTTTTTGGAGTGTTATT

Downstream 100 bases:

>100_bases
GAAGGGTTTTATCGGTAATTGTTTTAAATGAGCATGGGGTTTTATCTCGTATAGTAGGACTTTTTTCAGGAAGAGGTTAT
AATATTGACTCGCTTACAGT

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 566; Mature: 566

Protein sequence:

>566_residues
MKELSGSAMICEALKEENVKIVFGYPGGAALNIYDEIYNQKYFKHILVRHEQAALHAADAYARMSGEVGVAIVTSGPGFT
NTITGLATAYSDSIPLVLISGQVANSLIGTDAFQEIDAVGISRPCVKHNYLVTCIEEFPRILKEAFYIARSGRPGPVHID
VPKDVSATLGLWEYSKEISMKTYKPVYKGNSKQIKKFAELLKEAKRPLFYLGGGCISSNASEQIRELIKFTKIPAVETLM
ALGTLRSDDVFNLKMAGMHGSYAANMALSECDLLVSVGARFDDRITGKTSEFAKHATIVHVDIDPSSISKIINAHYPIVG
DIKEVLKELLEELKKENFNTTFKEWHETLKRYNELYPLSYEDSNEILKPQWVIEECAKMAPDARIITDVGQHQMWVAQFY
PFNYPRQLATSGGQGTMGYSLPAALGAKLAVGEEVVINFVGDGSVLMNIQELMTAYEYGIKTINIILNNAFLGMVRQWQS
MFYKEHFSQTDLSTQPDFIKIAQGFGCEGYEISNKEEFIQAFSQALKSDKTSLLNVKIDRFEDVLPMVPAGGAIYNMILP
KAKDRQ

Sequences:

>Translated_566_residues
MKELSGSAMICEALKEENVKIVFGYPGGAALNIYDEIYNQKYFKHILVRHEQAALHAADAYARMSGEVGVAIVTSGPGFT
NTITGLATAYSDSIPLVLISGQVANSLIGTDAFQEIDAVGISRPCVKHNYLVTCIEEFPRILKEAFYIARSGRPGPVHID
VPKDVSATLGLWEYSKEISMKTYKPVYKGNSKQIKKFAELLKEAKRPLFYLGGGCISSNASEQIRELIKFTKIPAVETLM
ALGTLRSDDVFNLKMAGMHGSYAANMALSECDLLVSVGARFDDRITGKTSEFAKHATIVHVDIDPSSISKIINAHYPIVG
DIKEVLKELLEELKKENFNTTFKEWHETLKRYNELYPLSYEDSNEILKPQWVIEECAKMAPDARIITDVGQHQMWVAQFY
PFNYPRQLATSGGQGTMGYSLPAALGAKLAVGEEVVINFVGDGSVLMNIQELMTAYEYGIKTINIILNNAFLGMVRQWQS
MFYKEHFSQTDLSTQPDFIKIAQGFGCEGYEISNKEEFIQAFSQALKSDKTSLLNVKIDRFEDVLPMVPAGGAIYNMILP
KAKDRQ
>Mature_566_residues
MKELSGSAMICEALKEENVKIVFGYPGGAALNIYDEIYNQKYFKHILVRHEQAALHAADAYARMSGEVGVAIVTSGPGFT
NTITGLATAYSDSIPLVLISGQVANSLIGTDAFQEIDAVGISRPCVKHNYLVTCIEEFPRILKEAFYIARSGRPGPVHID
VPKDVSATLGLWEYSKEISMKTYKPVYKGNSKQIKKFAELLKEAKRPLFYLGGGCISSNASEQIRELIKFTKIPAVETLM
ALGTLRSDDVFNLKMAGMHGSYAANMALSECDLLVSVGARFDDRITGKTSEFAKHATIVHVDIDPSSISKIINAHYPIVG
DIKEVLKELLEELKKENFNTTFKEWHETLKRYNELYPLSYEDSNEILKPQWVIEECAKMAPDARIITDVGQHQMWVAQFY
PFNYPRQLATSGGQGTMGYSLPAALGAKLAVGEEVVINFVGDGSVLMNIQELMTAYEYGIKTINIILNNAFLGMVRQWQS
MFYKEHFSQTDLSTQPDFIKIAQGFGCEGYEISNKEEFIQAFSQALKSDKTSLLNVKIDRFEDVLPMVPAGGAIYNMILP
KAKDRQ

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=26.1565836298932, Blast_Score=162, Evalue=9e-40,
Organism=Homo sapiens, GI21361361, Length=591, Percent_Identity=24.0270727580372, Blast_Score=145, Evalue=1e-34,
Organism=Escherichia coli, GI87081685, Length=572, Percent_Identity=46.5034965034965, Blast_Score=544, Evalue=1e-156,
Organism=Escherichia coli, GI1790104, Length=558, Percent_Identity=43.010752688172, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1786717, Length=530, Percent_Identity=32.6415094339623, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1787096, Length=553, Percent_Identity=26.5822784810127, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1788716, Length=564, Percent_Identity=26.9503546099291, Blast_Score=172, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17531299, Length=562, Percent_Identity=26.3345195729537, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17531301, Length=562, Percent_Identity=26.3345195729537, Blast_Score=146, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=592, Percent_Identity=25.1689189189189, Blast_Score=144, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6323755, Length=584, Percent_Identity=40.5821917808219, Blast_Score=436, Evalue=1e-123,
Organism=Saccharomyces cerevisiae, GI6320816, Length=558, Percent_Identity=25.089605734767, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320123, Length=575, Percent_Identity=23.304347826087, Blast_Score=117, Evalue=7e-27,
Organism=Saccharomyces cerevisiae, GI6321524, Length=585, Percent_Identity=25.2991452991453, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323073, Length=504, Percent_Identity=25.9920634920635, Blast_Score=108, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323163, Length=586, Percent_Identity=24.4027303754266, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI19922626, Length=572, Percent_Identity=26.7482517482518, Blast_Score=169, Evalue=6e-42,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62959; Mature: 62959

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELSGSAMICEALKEENVKIVFGYPGGAALNIYDEIYNQKYFKHILVRHEQAALHAADA
CCCCCCCHHHHHHHHHCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YARMSGEVGVAIVTSGPGFTNTITGLATAYSDSIPLVLISGQVANSLIGTDAFQEIDAVG
HHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHCCHHHHHHHHCC
ISRPCVKHNYLVTCIEEFPRILKEAFYIARSGRPGPVHIDVPKDVSATLGLWEYSKEISM
CCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHCCC
KTYKPVYKGNSKQIKKFAELLKEAKRPLFYLGGGCISSNASEQIRELIKFTKIPAVETLM
HHCCHHHCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHH
ALGTLRSDDVFNLKMAGMHGSYAANMALSECDLLVSVGARFDDRITGKTSEFAKHATIVH
HHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCEEEEE
VDIDPSSISKIINAHYPIVGDIKEVLKELLEELKKENFNTTFKEWHETLKRYNELYPLSY
EECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC
EDSNEILKPQWVIEECAKMAPDARIITDVGQHQMWVAQFYPFNYPRQLATSGGQGTMGYS
CCCCCCCCHHHHHHHHHHHCCCCEEEHHCCCCCEEEEEECCCCCCHHHHCCCCCCCCCCC
LPAALGAKLAVGEEVVINFVGDGSVLMNIQELMTAYEYGIKTINIILNNAFLGMVRQWQS
CHHHHCCHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHH
MFYKEHFSQTDLSTQPDFIKIAQGFGCEGYEISNKEEFIQAFSQALKSDKTSLLNVKIDR
HHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEHHH
FEDVLPMVPAGGAIYNMILPKAKDRQ
HHHHHCCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKELSGSAMICEALKEENVKIVFGYPGGAALNIYDEIYNQKYFKHILVRHEQAALHAADA
CCCCCCCHHHHHHHHHCCEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YARMSGEVGVAIVTSGPGFTNTITGLATAYSDSIPLVLISGQVANSLIGTDAFQEIDAVG
HHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHCCHHHHHHHHCC
ISRPCVKHNYLVTCIEEFPRILKEAFYIARSGRPGPVHIDVPKDVSATLGLWEYSKEISM
CCCCCHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHCCC
KTYKPVYKGNSKQIKKFAELLKEAKRPLFYLGGGCISSNASEQIRELIKFTKIPAVETLM
HHCCHHHCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHH
ALGTLRSDDVFNLKMAGMHGSYAANMALSECDLLVSVGARFDDRITGKTSEFAKHATIVH
HHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCEEEEE
VDIDPSSISKIINAHYPIVGDIKEVLKELLEELKKENFNTTFKEWHETLKRYNELYPLSY
EECCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCC
EDSNEILKPQWVIEECAKMAPDARIITDVGQHQMWVAQFYPFNYPRQLATSGGQGTMGYS
CCCCCCCCHHHHHHHHHHHCCCCEEEHHCCCCCEEEEEECCCCCCHHHHCCCCCCCCCCC
LPAALGAKLAVGEEVVINFVGDGSVLMNIQELMTAYEYGIKTINIILNNAFLGMVRQWQS
CHHHHCCHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHH
MFYKEHFSQTDLSTQPDFIKIAQGFGCEGYEISNKEEFIQAFSQALKSDKTSLLNVKIDR
HHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEHHH
FEDVLPMVPAGGAIYNMILPKAKDRQ
HHHHHCCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]