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The map label for this gene is pitA [C]

Identifier: 57238066

GI number: 57238066

Start: 1243068

End: 1244594

Strand: Direct

Name: pitA [C]

Synonym: CJE1328

Alternate gene names: 57238066

Gene position: 1243068-1244594 (Clockwise)

Preceding gene: 57238064

Following gene: 57238070

Centisome position: 69.92

GC content: 32.68

Gene sequence:

>1527_bases
ATGCAAAAAGACAACCTCATAGCTTTTGTTATTTTTATTATCAGTACTATCGCATTTGTTATTTGGGGATTTGGTTATAT
TTCGCAACATCAATTGATTCTTTTTATTCTTGCTTCTATATTTGGCATTTTTATGGCATTTAATATAGGTGGAAATGATG
TAGCCAATTCTTTTGGAACAAGCGTTGGCGCAAAAACAGTAACCATTAAACAAGCTTTAATTATAGCAGCTGTTTTTGAG
CTAAGCGGGGCAATCTTCGCAGGCGCAGAAGTTACTAAGACCATACGCAGCGGTATTGTTATTTTTCCAAATTCTTTAGA
TCCTATGCTTTTTGTTATCATTATGCTTGCTGCACTTTTAAGCTCTGGGGTTTGGATTTTTATTGCAACCAAGAAAGGCT
TACCTGTATCAACCACACACAGTATAGTTGGTGGGATTGTAGGTGCTAGTATTATGATGGGGCTTTTAAAATTTGATGGA
ATCCAAACACTCTCTATGGTTAAATGGAGTGAAATTTTAAGAATAGCTATTTCTTGGGTAGCTTCGCCTCTTTTAGGGGG
TATTGTAGCTTATATTATTTATTCATATATTGATAAGAAAATTTTAAAACCATCAGAAAAACTAAATGATGATTTGAAAA
ATATCAAAAAAGAAAGAAAAAAATTCAAAGAAGAATATTTTTTAAATTTAAAAACAAAATCCCAAGAAGAACAAATCAAA
GAACTTTCCGCTATAGCTTTGGATGAAGAAGAACAAGAAAATAATTTCTATAGAAATAAAATGAAGGAATTTAAAGAACA
AGAAAAAGATATAGATATTTACTCTATTTTAAAAACTCATATGCCAATCATTGCTTGTATTGCTGCTGCTATCATTTCGG
CAATGTTTTTATTTAAAGGTTTAAATAATGTTAGCACTTTGGATATTTTACAAAACTTTTGGATTATCGGAATTATAGGA
ACTATAAGTTATGTTGTAACATTTGCTATAGTCAAAATCGTTAAAAAAACAGAACTTAATAAAACTACTGATAGAATTTT
CTCATGGTTTCAAATATTTACAGCCTCTAGTTTTGCATTTTCACACGGAGCCAATGATATAGCCAATGCTATAGGTCCTT
TTGCAGCGATTTTAGATGTCTTAAAAAACGGCACAATTAACGCGACTTCTCCTGTACCTTTTGCTGCTTTAGCTATGTTT
GGAGTTGCTCTAGTTGTAGGACTTTGGTTTTTAGGTAAAGAAGTGATTACTACAGTGGGTTCAAAACTTGCAACCATACG
GCCTACTACTGGATTTTCTGCAGAACTTGGTGCAAGCATAGTTATACTCTTAGCTACACAATTTGGAATTCCTGTTAGCT
CCACCCATATACTCATAGGTGCTATCTTGGGTATAGGAGTGTATAATAAAAACGCAAATTGGATTATGATGAAGCCTATA
GGATTGGCTTGGATTATCACTTTACCAGCCGCTGGTATTATGGCAGCACTTGTATTTTTAGGATTTAAACTATCTTTAGG
AATTTAA

Upstream 100 bases:

>100_bases
ATAAAAAAATAATATAAATAAAAATTCCACAAAGACTAAAAAATAAAATTAGCAAATTTAAAGTAAATAAGTGTATAATT
AAAATAAAAAAGGGTTATTA

Downstream 100 bases:

>100_bases
GTAAAGCTTATTTTTCTAAAATATTTTTTCCTTTAATTATATGCATCTTAATCTTTCCAAAAAGTTTATCCTCAGAATAA
AGAGAACTTTTTGGAGCAAA

Product: phosphate transporter family protein

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MQKDNLIAFVIFIISTIAFVIWGFGYISQHQLILFILASIFGIFMAFNIGGNDVANSFGTSVGAKTVTIKQALIIAAVFE
LSGAIFAGAEVTKTIRSGIVIFPNSLDPMLFVIIMLAALLSSGVWIFIATKKGLPVSTTHSIVGGIVGASIMMGLLKFDG
IQTLSMVKWSEILRIAISWVASPLLGGIVAYIIYSYIDKKILKPSEKLNDDLKNIKKERKKFKEEYFLNLKTKSQEEQIK
ELSAIALDEEEQENNFYRNKMKEFKEQEKDIDIYSILKTHMPIIACIAAAIISAMFLFKGLNNVSTLDILQNFWIIGIIG
TISYVVTFAIVKIVKKTELNKTTDRIFSWFQIFTASSFAFSHGANDIANAIGPFAAILDVLKNGTINATSPVPFAALAMF
GVALVVGLWFLGKEVITTVGSKLATIRPTTGFSAELGASIVILLATQFGIPVSSTHILIGAILGIGVYNKNANWIMMKPI
GLAWIITLPAAGIMAALVFLGFKLSLGI

Sequences:

>Translated_508_residues
MQKDNLIAFVIFIISTIAFVIWGFGYISQHQLILFILASIFGIFMAFNIGGNDVANSFGTSVGAKTVTIKQALIIAAVFE
LSGAIFAGAEVTKTIRSGIVIFPNSLDPMLFVIIMLAALLSSGVWIFIATKKGLPVSTTHSIVGGIVGASIMMGLLKFDG
IQTLSMVKWSEILRIAISWVASPLLGGIVAYIIYSYIDKKILKPSEKLNDDLKNIKKERKKFKEEYFLNLKTKSQEEQIK
ELSAIALDEEEQENNFYRNKMKEFKEQEKDIDIYSILKTHMPIIACIAAAIISAMFLFKGLNNVSTLDILQNFWIIGIIG
TISYVVTFAIVKIVKKTELNKTTDRIFSWFQIFTASSFAFSHGANDIANAIGPFAAILDVLKNGTINATSPVPFAALAMF
GVALVVGLWFLGKEVITTVGSKLATIRPTTGFSAELGASIVILLATQFGIPVSSTHILIGAILGIGVYNKNANWIMMKPI
GLAWIITLPAAGIMAALVFLGFKLSLGI
>Mature_508_residues
MQKDNLIAFVIFIISTIAFVIWGFGYISQHQLILFILASIFGIFMAFNIGGNDVANSFGTSVGAKTVTIKQALIIAAVFE
LSGAIFAGAEVTKTIRSGIVIFPNSLDPMLFVIIMLAALLSSGVWIFIATKKGLPVSTTHSIVGGIVGASIMMGLLKFDG
IQTLSMVKWSEILRIAISWVASPLLGGIVAYIIYSYIDKKILKPSEKLNDDLKNIKKERKKFKEEYFLNLKTKSQEEQIK
ELSAIALDEEEQENNFYRNKMKEFKEQEKDIDIYSILKTHMPIIACIAAAIISAMFLFKGLNNVSTLDILQNFWIIGIIG
TISYVVTFAIVKIVKKTELNKTTDRIFSWFQIFTASSFAFSHGANDIANAIGPFAAILDVLKNGTINATSPVPFAALAMF
GVALVVGLWFLGKEVITTVGSKLATIRPTTGFSAELGASIVILLATQFGIPVSSTHILIGAILGIGVYNKNANWIMMKPI
GLAWIITLPAAGIMAALVFLGFKLSLGI

Specific function: Potential transporter for phosphate [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=160, Percent_Identity=35.625, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI5803173, Length=155, Percent_Identity=36.1290322580645, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17539280, Length=520, Percent_Identity=29.0384615384615, Blast_Score=225, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI71981576, Length=484, Percent_Identity=32.2314049586777, Blast_Score=209, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI25146401, Length=164, Percent_Identity=37.8048780487805, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI32566716, Length=161, Percent_Identity=39.1304347826087, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI17536725, Length=167, Percent_Identity=37.125748502994, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17557328, Length=219, Percent_Identity=27.3972602739726, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6319773, Length=162, Percent_Identity=41.9753086419753, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI21356511, Length=174, Percent_Identity=40.8045977011494, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 55320; Mature: 55320

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKDNLIAFVIFIISTIAFVIWGFGYISQHQLILFILASIFGIFMAFNIGGNDVANSFGT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
SVGAKTVTIKQALIIAAVFELSGAIFAGAEVTKTIRSGIVIFPNSLDPMLFVIIMLAALL
CCCCCHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHH
SSGVWIFIATKKGLPVSTTHSIVGGIVGASIMMGLLKFDGIQTLSMVKWSEILRIAISWV
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ASPLLGGIVAYIIYSYIDKKILKPSEKLNDDLKNIKKERKKFKEEYFLNLKTKSQEEQIK
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHH
ELSAIALDEEEQENNFYRNKMKEFKEQEKDIDIYSILKTHMPIIACIAAAIISAMFLFKG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LNNVSTLDILQNFWIIGIIGTISYVVTFAIVKIVKKTELNKTTDRIFSWFQIFTASSFAF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SHGANDIANAIGPFAAILDVLKNGTINATSPVPFAALAMFGVALVVGLWFLGKEVITTVG
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SKLATIRPTTGFSAELGASIVILLATQFGIPVSSTHILIGAILGIGVYNKNANWIMMKPI
CHHEEECCCCCCCHHHHHHHHHEEHHHCCCCCCCHHHHHHHHHHHCEEECCCCEEEECCC
GLAWIITLPAAGIMAALVFLGFKLSLGI
CCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQKDNLIAFVIFIISTIAFVIWGFGYISQHQLILFILASIFGIFMAFNIGGNDVANSFGT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCC
SVGAKTVTIKQALIIAAVFELSGAIFAGAEVTKTIRSGIVIFPNSLDPMLFVIIMLAALL
CCCCCHHHHHHHHHHHHHHHHCCCHHCCHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHH
SSGVWIFIATKKGLPVSTTHSIVGGIVGASIMMGLLKFDGIQTLSMVKWSEILRIAISWV
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
ASPLLGGIVAYIIYSYIDKKILKPSEKLNDDLKNIKKERKKFKEEYFLNLKTKSQEEQIK
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHH
ELSAIALDEEEQENNFYRNKMKEFKEQEKDIDIYSILKTHMPIIACIAAAIISAMFLFKG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LNNVSTLDILQNFWIIGIIGTISYVVTFAIVKIVKKTELNKTTDRIFSWFQIFTASSFAF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SHGANDIANAIGPFAAILDVLKNGTINATSPVPFAALAMFGVALVVGLWFLGKEVITTVG
HCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SKLATIRPTTGFSAELGASIVILLATQFGIPVSSTHILIGAILGIGVYNKNANWIMMKPI
CHHEEECCCCCCCHHHHHHHHHEEHHHCCCCCCCHHHHHHHHHHHCEEECCCCEEEECCC
GLAWIITLPAAGIMAALVFLGFKLSLGI
CCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]