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The map label for this gene is dctA [H]

Identifier: 57238064

GI number: 57238064

Start: 1240825

End: 1242123

Strand: Direct

Name: dctA [H]

Synonym: CJE1326

Alternate gene names: 57238064

Gene position: 1240825-1242123 (Clockwise)

Preceding gene: 57238046

Following gene: 57238066

Centisome position: 69.79

GC content: 33.49

Gene sequence:

>1299_bases
GTGATTATAGGCATTATTACAGGTATTGTACTTGGCTATACAGATAAAGAATTAGCCATTGCTAGCAAACCAGGAGTTGA
TTATTTTATAGGTGCTTTAAAGGTTCTTATAGGCCCTATTATTTTTGTTACTTTGGTTTTGGGCATCATTAGTCTTGAAA
GTTTAAAAAAAGTAGGTAGCATTGGTGCTAAAGCGGTTATTTACTTTGAAGTTGTAAGTACTTTAGCACTTGCTATAGGT
ATTTTTATGGCAAATGTTATGCAACCAGGCCATGGAATGAATCTTGATCCAAGTCAACTTGATACAAAAAGCGTTCAAAA
ATACATTTCTCAAACCACAGAAGTAAGTGCGAGCTCTGAGATTATGCATATTTTAAAAGATGCTATGCCTACTGATATTA
TCACACCTTTTACAGAAGGAAAAACCATACAAGTTTTAGTTATTGCTATAATTACTGCTTTGATTATTTCTTTAATGAGA
ATAGAAGACAAACAGGCAATTCAAAGAGTTTTTGAAGTGGTACAAAATTTTGTTTTTAAAATTCTACAAATCATTATGTA
TTTTAGTCCTATTGCCGCCTTTTCAGCAATGGCTGTACTCATAGCTCAATACGGAATTGGCTCTTTGATCAACTTAGCAT
ATTTACTTTTAGTAATGCTGATTTCATGTCTTGTTTTTATATTTGGAATCTTAGGTCTGATTTGTTATTTTGCAAAAGTA
AATATCTTCAAATTTATGAGATTTATCTCAAGAGAAGTATTGATCGTTTTTGCAACCAGTTCAAGCGAGTCTGCACTTGC
TCCTTTAATGAGAAAACTAGAAAAAGCAGGACTTTCTAAAGCAACAGTCGGACTTGTTTTACCAACGGGATATAGTTTTA
ATCTTGATTGTACAAATATTTATCTAGCTATGAGCTTAATCTTTCTAGCACAAGCTTTCAATGTAAATTTAAGTTTAGCA
CACGAAATCAGTATTTTAATAGTATTAATGATAGCCTCAAAAGGTGCAGTAGGTGTAACTGGATCAGGTTTTATAGTGCT
TGGAAGTACGCTTGCGGCTCTAGGAAATATGGAAATTTCAGAAGCAAATGCAACCTTAGCACAAGTTCTACCAGTAGCTG
CTATAGGTGTTCTTTTAGGTGTTGATAAATTTATGTCTGAAATGAGAGCGGTTGGAAATCTTTGCGGAAATTCAGTAGCA
GCTTTAATCGTTGCTATCTGGGATAAACAAATTGATTGGGAAAAATTCCGCTACGCTATGGACAATCCAGAAAAATTTCA
TAATGCTGGAATGAACTAA

Upstream 100 bases:

>100_bases
AGATCAAAAGGAGTGAATAAACTTGGAAACTTTAAGTTAAACTTTAGCTGAAAGAAAACCGCCTTTATATAAACGTATTA
TCAAAAGTCTTGGATTTTGG

Downstream 100 bases:

>100_bases
TGAGTTCTTTGGAGGAAATCATATCGATTAACCTCCTCTAAAGTATTATCTTTTTTTCTAAATTGCTTTAAAAAACTTTC
ATTGAAATTAAAACTAAATC

Product: C4-dicarboxylate transport protein

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MIIGIITGIVLGYTDKELAIASKPGVDYFIGALKVLIGPIIFVTLVLGIISLESLKKVGSIGAKAVIYFEVVSTLALAIG
IFMANVMQPGHGMNLDPSQLDTKSVQKYISQTTEVSASSEIMHILKDAMPTDIITPFTEGKTIQVLVIAIITALIISLMR
IEDKQAIQRVFEVVQNFVFKILQIIMYFSPIAAFSAMAVLIAQYGIGSLINLAYLLLVMLISCLVFIFGILGLICYFAKV
NIFKFMRFISREVLIVFATSSSESALAPLMRKLEKAGLSKATVGLVLPTGYSFNLDCTNIYLAMSLIFLAQAFNVNLSLA
HEISILIVLMIASKGAVGVTGSGFIVLGSTLAALGNMEISEANATLAQVLPVAAIGVLLGVDKFMSEMRAVGNLCGNSVA
ALIVAIWDKQIDWEKFRYAMDNPEKFHNAGMN

Sequences:

>Translated_432_residues
MIIGIITGIVLGYTDKELAIASKPGVDYFIGALKVLIGPIIFVTLVLGIISLESLKKVGSIGAKAVIYFEVVSTLALAIG
IFMANVMQPGHGMNLDPSQLDTKSVQKYISQTTEVSASSEIMHILKDAMPTDIITPFTEGKTIQVLVIAIITALIISLMR
IEDKQAIQRVFEVVQNFVFKILQIIMYFSPIAAFSAMAVLIAQYGIGSLINLAYLLLVMLISCLVFIFGILGLICYFAKV
NIFKFMRFISREVLIVFATSSSESALAPLMRKLEKAGLSKATVGLVLPTGYSFNLDCTNIYLAMSLIFLAQAFNVNLSLA
HEISILIVLMIASKGAVGVTGSGFIVLGSTLAALGNMEISEANATLAQVLPVAAIGVLLGVDKFMSEMRAVGNLCGNSVA
ALIVAIWDKQIDWEKFRYAMDNPEKFHNAGMN
>Mature_432_residues
MIIGIITGIVLGYTDKELAIASKPGVDYFIGALKVLIGPIIFVTLVLGIISLESLKKVGSIGAKAVIYFEVVSTLALAIG
IFMANVMQPGHGMNLDPSQLDTKSVQKYISQTTEVSASSEIMHILKDAMPTDIITPFTEGKTIQVLVIAIITALIISLMR
IEDKQAIQRVFEVVQNFVFKILQIIMYFSPIAAFSAMAVLIAQYGIGSLINLAYLLLVMLISCLVFIFGILGLICYFAKV
NIFKFMRFISREVLIVFATSSSESALAPLMRKLEKAGLSKATVGLVLPTGYSFNLDCTNIYLAMSLIFLAQAFNVNLSLA
HEISILIVLMIASKGAVGVTGSGFIVLGSTLAALGNMEISEANATLAQVLPVAAIGVLLGVDKFMSEMRAVGNLCGNSVA
ALIVAIWDKQIDWEKFRYAMDNPEKFHNAGMN

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI4827012, Length=476, Percent_Identity=23.109243697479, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI169790839, Length=465, Percent_Identity=23.2258064516129, Blast_Score=102, Evalue=6e-22,
Organism=Homo sapiens, GI194239697, Length=470, Percent_Identity=25.1063829787234, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI66773030, Length=412, Percent_Identity=23.5436893203883, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI40254478, Length=438, Percent_Identity=24.6575342465753, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI21314632, Length=434, Percent_Identity=23.7327188940092, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI262359914, Length=429, Percent_Identity=21.9114219114219, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI5032093, Length=402, Percent_Identity=24.3781094527363, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI223468566, Length=238, Percent_Identity=26.4705882352941, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI223468564, Length=238, Percent_Identity=26.4705882352941, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1789947, Length=418, Percent_Identity=45.4545454545455, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI1790514, Length=420, Percent_Identity=33.0952380952381, Blast_Score=248, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI17537407, Length=442, Percent_Identity=26.0180995475113, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI71983099, Length=439, Percent_Identity=23.9179954441913, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71983106, Length=439, Percent_Identity=23.9179954441913, Blast_Score=101, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71996953, Length=486, Percent_Identity=23.6625514403292, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI193206505, Length=461, Percent_Identity=24.295010845987, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI193206654, Length=444, Percent_Identity=23.6486486486486, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24583025, Length=441, Percent_Identity=22.2222222222222, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17137668, Length=441, Percent_Identity=22.2222222222222, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24583023, Length=441, Percent_Identity=22.2222222222222, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281360483, Length=457, Percent_Identity=23.6323851203501, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17137666, Length=457, Percent_Identity=23.6323851203501, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281360481, Length=457, Percent_Identity=23.6323851203501, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46586; Mature: 46586

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHHHCCCCC
>Mature Secondary Structure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CCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA